scholarly journals Stilbene synthase gene expression causes changes in flower colour and male sterility in tobacco

1997 ◽  
Vol 11 (3) ◽  
pp. 489-498 ◽  
Author(s):  
Regina Fischer ◽  
Ina Budde ◽  
Rudiger Hain
Keyword(s):  
Gene Expression ◽  
Male Sterility ◽  
Flower Colour ◽  
Stilbene Synthase ◽  
Stilbene Synthase Gene

scholarly journals Molecular Basis of Ergosterol-Induced Protection of Grape Against Botrytis cinerea: Induction of Type I LTP Promoter Activity, WRKY, and Stilbene Synthase Gene Expression

2006 ◽  
Vol 19 (10) ◽  
pp. 1103-1112 ◽  
Author(s):  
Laurent Laquitaine ◽  
Eric Gomès ◽  
Julie François ◽  
Chloé Marchive ◽  
Stéphanie Pascal ◽  
...  
Keyword(s):  
Gene Expression ◽  
Botrytis Cinerea ◽  
Molecular Basis ◽  
Stilbene Synthase ◽  
Gus Activity ◽  
Cell Suspensions ◽  
Type I ◽  
Lipid Transfer ◽  
Tobacco Plants ◽  
Stilbene Synthase Gene

Type I lipid transfer proteins (LTPs) are basic, 9-kDa cystein-rich proteins believed to be involved in plant defense mechanisms. A 2,100-bp fragment containing the coding region of Vitis vinifera lipid transfer protein 1 (VvLTP1) and 1,420-bp of its promoter region was isolated by screening a grape genomic library. In silico analysis revealed several putative, defense-related, cis-regulatory elements such as W- and MYB-boxes, involved in the binding of WRKY and MYB transcription factors, respectively. The 5′-truncated versions of the VvLTP1 promoter were generated, cloned in front of the β-glucuronidase (GUS) reporter gene, and introduced in tobacco plants and grapevine cell suspensions using Agrobacterium spp. Single MYB- and the W-boxes identified on the 0.250-kbp fragment were sufficient to induce GUS activity in transgenic tobacco plants after transient expression of MYB and WRKY. Ergosterol, a nonspecific fungal elicitor, induced GUS activity in transgenic grapevine cell suspensions transformed with the 1,420- and 750-bp promoter containing a palindromic arrangement of two Wboxes but not the 650- or 250-bp fragment, where only one W-box was present. Moreover, ergosterol triggered WRKY, VvLTP1, and stilbene synthase gene expression in grape plantlets and enhanced protection against Botrytis cinerea. The molecular basis of ergosterol-induced protection is discussed.

scholarly journals The grapevine R2R3-type MYB transcription factor VdMYB1 positively regulates defense responses by activating the stilbene synthase gene 2 (VdSTS2)

2019 ◽  
Vol 19 (1) ◽  
Author(s):  
Yihe Yu ◽  
Dalong Guo ◽  
Guirong Li ◽  
Yingjun Yang ◽  
Guohai Zhang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcription Factor ◽  
Transcriptional Activation ◽  
Molecular Mechanisms ◽  
Plant Protection ◽  
Defense Responses ◽  
Stilbene Synthase ◽  
Myb Transcription Factor ◽  
Wide Range ◽  
Grapevine Leaves

Abstract Background Resveratrol is a naturally occurring plant stilbene that exhibits a wide range of valuable biological and pharmacological properties. Although the beneficial effects of trans-resveratrol to human health and plant protection against fungal pathogens and abiotic stresses are well-established, yet little is known about the molecular mechanisms regulating stilbene biosynthesis in plant defense progress. Results Here, we cloned and identified the Chinese wild grape (Vitis davidii) R2R3-MYB transcription factor VdMYB1, which activates defense responses against invading pathogen. VdMYB1 transcripts were significantly upregulated after inoculation with the grapevine powdery mildew fungus Erysiphe necator (Schw.) Burr. Transient expression analysis using onion epidermal cells and Arabidopsis thaliana protoplasts showed that VdMYB1 was localized in the nucleus. Yeast one-hybrid assays revealed that VdMYB1 acts as a transcriptional activator. Grapevine leaves transiently overexpressing VdMYB1 showed a lower number of fungal conidiophores compared with wild-type leaves. Overexpression of VdMYB1 in grapevine leaves did not alter the expression of genes in salicylic acid- and jasmonate-dependent pathways, but affected the expression of stilbene synthase (STS) genes, key regulators of flavonoid metabolism. Results of electrophoretic mobility shift assays and in vivo transcriptional activation assays showed that VdMYB1 binds to the MYB binding site (MYBBS) in the STS2 gene promoter, thus activating STS2 transcription. In heterologous expression assays using tobacco leaves, VdMYB1 activated STS2 gene expression and increased the accumulation of resveratrol. Conclusions Our study showed that VdMYB1 activates STS2 gene expression to positively regulate defense responses, and increases the content of resveratrol in leaves.

The molecular regulation of stilbene phytoalexin biosynthesis in Vitis vinifera during grape berry development

2000 ◽  
Vol 27 (7) ◽  
pp. 723 ◽  
Author(s):  
Anthony J. Bais ◽  
Peter J. Murphy ◽  
Ian B. Dry
Keyword(s):  
Gene Expression ◽  
Vitis Vinifera ◽  
Stilbene Synthase ◽  
Grape Berry ◽  
Molecular Regulation ◽  
Vitis Vinifera L ◽  
Uv Treatment ◽  
Grape Berries ◽  
Uv Induction ◽  
Phytoalexin Biosynthesis

The molecular regulation of stilbene phytoalexin biosynthesis in developing Vitis vinifera L. grape berries was investigated using a UV induction system. Berries were collected at 1, 5, 10 and 16 weeks post-flowering from the cultivars Shiraz, Semillon, Cabernet Sauvignon and Chardonnay and the skins analysed for resveratrol production following irradiation with UV-C light. The rate and maximal level of resveratrol accumulation increased markedly in berries sampled from 1–5 weeks post-flowering and then dramatically declined in maturing berries sampled from 10–16 weeks post-flowering in all cultivars. In berries sampled at 1 and 5 weeks post-flowering, maximal levels of resveratrol accumulation were recorded at incubation periods of 24 and 48 h respectively whereas maximal resveratrol levels were not recorded in week 16 berry skins until 72 h after UV-treatment. Gene expression analysis indicated that stilbene synthase (STS) mRNA accumulated within 4–8 h of UV treatment in berries sampled at 1 and 5 weeks post-flowering, but did not increase in week 16 berries until 24–48 h following UV-irradiation. Furthermore, the overall level of STS gene expression declined in berries sampled 10–16 weeks post-flowering. The results demonstrate that inducible stilbene accumulation in ripening grape berries is highly regulated at the level of STS gene transcription. This decline in inducible STS gene expression may be a major factor contributing to the increased susceptibility of ripening grape berries to Botrytis cinerea infection.

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