Optimization of the experimental conditions for macrolide antibiotics in high performance liquid chromatography by using response surface methodology and determination of tylosin in milk samples

2011 ◽  
Vol 66 (9) ◽  
pp. 838-847 ◽  
Author(s):  
Senem Şanli ◽  
İsmail M. Palabiyik ◽  
Nurullah Şanli ◽  
Zeynep B. Guzel-Seydim ◽  
Güleren Alsancak
Keyword(s):  
Response Surface Methodology ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Response Surface ◽  
High Performance ◽  
Macrolide Antibiotics ◽  
Experimental Conditions ◽  
Milk Samples

Dispersive liquid–liquid microextraction for preconcentration and determination of phenytoin in real samples using response surface methodology-high performance liquid chromatography

RSC Advances ◽  
2014 ◽  
Vol 4 (107) ◽  
pp. 62190-62196 ◽  
Author(s):  
Eslam Pourbasheer ◽  
Samira Sadafi ◽  
Mohammad Reza Ganjali ◽  
Maryam Abbasghorbani
Keyword(s):  
Response Surface Methodology ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Response Surface ◽  
High Performance ◽  
Real Samples ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

In the present study, dispersive liquid–liquid microextraction (DLLME) was developed for preconcentration and determination of phenytoin in real samples by high performance liquid chromatography (HPLC).

Simultaneous determination of five macrolide antibiotics in meat by high-performance liquid chromatography

1998 ◽  
Vol 812 (1-2) ◽  
pp. 295-302 ◽  
Author(s):  
Masakazu Horie ◽  
Koichi Saito ◽  
Rie Ishii ◽  
Terumitu Yoshida ◽  
Yukari Haramaki ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Macrolide Antibiotics

scholarly journals Salting-out assisted liquid–liquid extraction coupled with high-performance liquid chromatography for the determination of vitamin D3 in milk samples

2019 ◽  
Vol 6 (8) ◽  
pp. 190952 ◽  
Author(s):  
Nur Hidayah Sazali ◽  
Anas Alshishani ◽  
Bahruddin Saad ◽  
Ker Yin Chew ◽  
Moi Me Chong ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Vitamin D3 ◽  
High Performance ◽  
Liquid Extraction ◽  
Sample Treatment ◽  
Salting Out ◽  
Milk Samples ◽  
Liquid Liquid Extraction

In this study, salting-out assisted liquid–liquid extraction (SALLE) as a simple and efficient extraction technique followed by high-performance liquid chromatography (HPLC) was employed for the determination of vitamin D3 in milk samples. The sample treatment is based on the use of water-miscible acetonitrile as the extractant and acetonitrile phase separation under high-salt conditions. Under the optimum conditions, acetonitrile and ammonium sulfate were used as the extraction solvent and salting-out agent, respectively. The vitamin D3 extract was separated using Hypersil ODS (250x i.d 4.6 mm, 5 µm) HPLC column that was coupled with diode array detector. Vitamin D2 was used as internal standard (IS) to offset any variations in chromatographic conditions. The vitamin D3 and the IS were eluted in 18 min. Good linearity ( r 2 > 0.99) was obtained within the range of 25–600 ng g −1 with the limit of detection of 15 ng g −1 and limit of quantification of 25 ng g −1 . The validated method was applied for the determination of vitamin D3 in milk samples. The recoveries for spiked samples were from 94.4 to 113.5%.

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