The role of the MEK/ERK pathway in regulation of HDACI-induced senescence of transformed rat embryo fibroblasts

2014 ◽  
Vol 8 (5) ◽  
pp. 374-383
Author(s):  
E. Yu. Kochetkova ◽  
T. V. Bykova ◽  
S. G. Zubova ◽  
T. V. Pospelova
Keyword(s):  
Erk Pathway ◽  
Rat Embryo ◽  
Embryo Fibroblasts

scholarly journals The oncogene BRAFV600E is associated with a high risk of recurrence and less differentiated papillary thyroid carcinoma due to the impairment of Na+/I− targeting to the membrane

2006 ◽  
Vol 13 (1) ◽  
pp. 257-269 ◽  
Author(s):  
G Riesco-Eizaguirre ◽  
P Gutiérrez-Martínez ◽  
M A García-Cabezas ◽  
M Nistal ◽  
P Santisteban
Keyword(s):  
High Risk ◽  
Papillary Thyroid Carcinoma ◽  
Thyroid Carcinoma ◽  
Prognostic Impact ◽  
Erk Pathway ◽  
Papillary Thyroid ◽  
Thyroid Cells ◽  
Genetic Event ◽  
Total Body

The oncogene BRAFV600E is the most frequent genetic event in papillary thyroid carcinoma (PTC) but its prognostic impact still remains to be elucidated. We evaluated a representative series of 67 individuals with PTC who underwent total thyroidectomy. BRAF-positive tumours correlated with early recurrences (32% vs 7.6%; P=0.02) during a median postoperative follow-up period of 3 years. Interestingly, within the recurrences, a significant majority had negative radioiodine (131I) total body scans, predicting a poorer outcome as treatment with 131I is not effective. This last observation led us to investigate the role of BRAFV600E and the MEK-ERK pathway in thyroid dedifferentiation, particularly in Na+/I− symporter (NIS) impairment, as this thyroid-specific plasma membrane glycoprotein mediates active transport of I− into the thyroid follicular cells. A subset of 60 PTC samples was evaluated for NIS immunoreactivity and, accordingly, we confirmed a significant low NIS expression and impaired targeting to membranes in BRAF-positive samples (3.5% vs 30%; P=0.005). Furthermore, experiments with differentiated PCCl3 thyroid cells demonstrated that transient expression of BRAFV600E sharply impaired both NIS expression and targeting to membrane and, surprisingly, this impairment was not totally dependent on the MEK-ERK pathway. We have concluded that BRAFV600E is a new prognostic factor in PTC that correlates with a high risk of recurrences and less differentiated tumours due to the loss of NIS-mediated 131I uptake.

The role of the map kinase ERK pathway in the antipsychotic actions of clozapine

2003 ◽  
Vol 60 (1) ◽  
pp. 309
Author(s):  
J.L. Browning ◽  
K.A. Young ◽  
L.A. Holcomb ◽  
D.H. Jones ◽  
P.B. Hicks
Keyword(s):  
Map Kinase ◽  
Erk Pathway

scholarly journals Rapid catabolism of tyrosine-O-sulphated proteins and the formation of free tyrosine O-sulphate as an end product in rat embryo fibroblasts

1987 ◽  
Vol 243 (2) ◽  
pp. 555-559 ◽  
Author(s):  
M C Liu ◽  
M Suiko ◽  
F Lipmann
Keyword(s):  
Incubation Medium ◽  
Fresh Medium ◽  
Rat Embryo ◽  
Time Points ◽  
Rate Of Increase ◽  
Embryo Fibroblasts

Rat embryo fibroblasts, line 3Y1, were prelabelled for 24 h with [35S]sulphate and incubated in fresh medium without [35S]sulphate. A rapid efflux of the overall 35S-labelled compounds from the cells into the medium was observed. After 9 h of incubation, about 50% of the total 35S radioactivity appeared in the medium and up to 84.3% did so at the end of a 48 h incubation. Determination of [35S]sulphated macromolecules present in both the cell-associated and the incubation-medium fractions at different time points during incubation indicated that the majority of the 35S-labelled compounds released from the cells were low-Mr products derived from digestion of the [35S]sulphated macromolecules. Further analysis for tyrosine-O-[35S]sulphated proteins, which constituted only a small fraction of the overall [35S]sulphated macromolecules, showed that, after 9 h of incubation, there was a 65% decrease in the cell-associated fraction, and only 16.4% remained after 48 h. During that time, an amount equivalent to 20.7% of the cell-associated tyrosine-O-[35S]sulphated proteins originally present was released into the medium. Free tyrosine O-[35S]sulphate was generated in the cells and excreted into the incubation medium. Its rate of increase with time, however, was slow, and could account for only 12.4% of the tyrosine-O-[35S]sulphated proteins catabolized at the end of the 48 h incubation.

The mRNA 5' cap-binding protein, eIF-4E, cooperates with v-myc or E1A in the transformation of primary rodent fibroblasts

1992 ◽  
Vol 12 (3) ◽  
pp. 1234-1238
Author(s):  
A Lazaris-Karatzas ◽  
N Sonenberg
Keyword(s):  
Signal Transduction ◽  
Binding Protein ◽  
Signal Transduction Pathway ◽  
Transduction Pathway ◽  
Rat Embryo ◽  
Present Evidence ◽  
P21 Ras ◽  
Embryo Fibroblasts ◽  
Common Signal

We present evidence that eIF-4E, the mRNA 5' cap-binding protein, cooperates with two immortalizing oncogenes, v-myc and E1A, to cause transformation of rat embryo fibroblasts. eIF-4E alone can transform rat embryo fibroblasts when selection is applied. The pattern of transformation by eIF-4E is similar to that of p21 Ras, raising the possibility that eIF-4E shares a common signal transduction pathway with p21 Ras.

scholarly journals Transferrin Uptake by Cultured Rat Embryo Fibroblasts

2005 ◽  
Vol 115 (3) ◽  
pp. 611-618 ◽  
Author(s):  
Jean-Noël OCTAVE ◽  
Yves-Jacques SCHNEIDER ◽  
André TROUET ◽  
Robert R. CRICHTON
Keyword(s):  
Rat Embryo ◽  
Embryo Fibroblasts ◽  
Transferrin Uptake
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