Fluorescence Reagents for Labelling of Biomolecules. Part III. Study of the Reactions of 2- and 4-Substituted 9-Isothiocyanatoacridines with Glycine
1994 ◽
Vol 59
(7)
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pp. 1682-1690
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Keyword(s):
H Nmr
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Kinetics of nucleophilic addition reaction of 2- and 4-substituted 9-isothiocyanatoacridines I - VII with glycine in buffered aqueous dimethylformamide has been studied. The addition products, N-(9-acridinylthiocarbamoyl)glycines VIII - XIV, were characterized by IR, UV, 1H NMR, mass and fluorescence spectra. Derivatives VIII, X and XII exhibited higher fluorescence intensity than the starting isothiocyanates; the highest fluorescence was found for the unsubstituted compound X. The reaction mechanism is discussed on the basis of properties of the reaction products and kinetic characteristics.
1975 ◽
Vol 40
(9)
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pp. 2838-2844
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Keyword(s):
1994 ◽
Vol 59
(12)
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pp. 2632-2640
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1986 ◽
Vol 108
(10)
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pp. 2517-2527
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Keyword(s):
1976 ◽
Vol 17
(36)
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pp. 3109-3112
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