scholarly journals Rubella: a method for rapid diagnosis of a recent infection by demonstration of the IgM antibodies.

BMJ ◽  
1968 ◽  
Vol 1 (5586) ◽  
pp. 221-223 ◽  
Author(s):  
T. Vesikari ◽  
A. Vaheri
Keyword(s):  
Rapid Diagnosis ◽  
Recent Infection ◽  
Igm Antibodies

scholarly journals Evaluation of the TGS TA system for the detection of anti-Toxoplasma antibodies

2017 ◽  
Vol 32 (1) ◽  
Author(s):  
Olivia Arpino ◽  
Annalisa Cianflone ◽  
Maria Teresa Manco ◽  
Alessia Paganini ◽  
Massimo De Paschale ◽  
...  
Keyword(s):  
High Avidity ◽  
Past Infection ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Igg Avidity ◽  
Avidity Test ◽  
Group 3 ◽  
Group 2 ◽  
Group 1

<em>Background and aims.</em> The aim of the present study was to evaluate the new chemiluminescence TGS TA system of Technogenetics (Milan, Italy) for detecting anti-Toxoplasma IgG and IgM antibodies and IgG avidity. The TGS TA system was compared with our chemiluminescence routinely used system, LIAISON XL, supplied by Diasorin (Saluggia, Italy), for the detection of IgG and IgM antibodies. Only in positive IgM samples (retrospective study) and for the IgG avidity (if existent), TGS TA system was compared to an Enzyme Linked Fluorescent Assay (ELFA) test (VIDAS, BioMérieux, Marcy-l’Étoile, France). <br /><em>Materials and methods</em>. Three hundred and one sera samples, from women who came to our centre for the routine follow up pregnancy, were examined with the TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 106 were non-immune women (Group 1), 100 were pregnant with past infection (Group 2) and 95 were pregnant with positive or equivocal IgM (82 with positive IgG and 13 with negative IgG) (Group 3). <br /><em>Results</em>. The overall concordance of the IgG results between LIAISON XL and TGS TA was 99.3%: 100% in Group 1, 98% in Group 2 and 100% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 93.9%: 100% in Group 1, 94% in Group 2 and 82.8% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the ELFA and TGS TA tests was 81.7%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 92.7%: 100% in Group 1, 92.0% in Group 2 and 78.9% in Group 3. <br /><em>Conclusions</em>. The overall concordance of IgG antibodies is excellent for both protocols while for IgM antibodies is very high in the first group and lower in the third group, due to the presence of non-specific IgM subjects in this group. The TGS TA avidity test seems to predict ealier the maturation of the IgG compared to the ELFA test since many samples with low avidity with the ELFA were seen with moderate avidity with TGS TA and all those with borderline avidity with the ELFA were seen with high avidity with TGS TA. This system shows to be a valuable tool with overall good clinical correlation and able to clearly identify nonspecific subjects, those with a non-recent infection.

scholarly journals Evaluation of the TGS TA system for the detection of anti-rubella antibodies

2017 ◽  
Vol 32 (2) ◽  
Author(s):  
Olivia Arpino ◽  
Annalisa Cianflone ◽  
Maria Teresa Manco ◽  
Alessia Paganini ◽  
Massimo De Paschale ◽  
...  
Keyword(s):  
Elisa Test ◽  
Childbearing Age ◽  
Past Infection ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Igg Avidity ◽  
Group 3 ◽  
Group 2 ◽  
Sera Samples ◽  
Group 1

<p><em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting antirubella IgG and IgM antibodies and IgG avidity. TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and IgM antibodies. Only in positive IgM samples (retrospective study), TGS TA system was compared to an ELFA IgM test and with an ELISA test for the IgG avidity (if existent).<br /><em>Materials and methods:</em> Two hundred and seventy six sera samples from women were examined with TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 112 were of childbearing age and non-immune women (Group 1), 106 were pregnant with past infection or vaccinated (Group 2) and 49 were pregnant with positive or equivocal IgM (Group 3). <br /><em>Results</em>: The overall concordance of the IgG results between LIAISON XL and TGS TA was 93.3%: 86.6% in Group 1, 97.2% in Group 2 and 100% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 89.0%: 100% in Group 1, 100% in Group 2 and 35.6% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the ELISA and TGS TA tests was 85.7%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 97.4%: 86.6% in Group 1, 97.2% in Group 2 and 85.7% in Group 3. <br /><em>Conclusions</em>: TGA TS system shows to be a valuable tool with overall good clinical correlation and able to clearly identify nonspecific subjects, those with a non-recent infection or those who are vaccinated. The TGS TA test also seems to be especially sensitive in indicating vaccinated subjects with low IgG levels as immune.</p>

scholarly journals Equine seroprevalence of West Nile virus antibodies in the UK in 2019

2020 ◽  
Vol 13 (1) ◽  
Author(s):  
Arran J. Folly ◽  
Elisabeth S. L. Waller ◽  
Fiona McCracken ◽  
Lorraine M. McElhinney ◽  
Helen Roberts ◽  
...  
Keyword(s):  
West Nile Virus ◽  
Rna Virus ◽  
Clinical History ◽  
Immunoglobulin M ◽  
Serum Samples ◽  
West Nile ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Epidemiological Surveys ◽  
The Uk

Abstract Background West Nile virus (WNV) is a single-stranded RNA virus that can cause neurological disease in both humans and horses. Due to the movement of competent vectors and viraemic hosts, WNV has repeatedly emerged globally and more recently in western Europe. Within the UK, WNV is a notifiable disease in horses, and vaccines against the virus are commercially available. However, there has been no investigation into the seroprevalence of WNV in the UK equine population to determine the extent of vaccination or to provide evidence of recent infection. Methods Equine serum samples were obtained from the Animal and Plant Health Agency’s equine testing service between August and November 2019. A total of 988 serum samples were selected for horses resident in South East England. WNV seroprevalence was determined using two enzyme-linked immunosorbent assays (ELISAs) to detect total flavivirus antibodies and WNV-specific immunoglobulin M (IgM) antibodies. Positive IgM results were investigated by contacting the submitting veterinarian to establish the clinical history or evidence of prior vaccination of the horses in question. Results Within the cohort, 274 samples tested positive for flavivirus antibodies, of which two subsequently tested positive for WNV-specific IgM antibodies. The follow-up investigation established that both horses had been vaccinated prior to serum samples being drawn, which resulted in an IgM-positive response. All the samples that tested positive by competition ELISA were from horses set to be exported to countries where WNV is endemic. Consequently, the positive results were likely due to previous vaccination. In contrast, 714 samples were seronegative, indicating that the majority of the UK equine population may be susceptible to WNV infection. Conclusions There was no evidence for cryptic WNV infection in a cohort of horses sampled in England in 2019. All IgM-seropositive cases were due to vaccination; this should be noted for future epidemiological surveys in the event of a disease outbreak, as it is not possible to distinguish vaccinated from infected horses without knowledge of their clinical histories.

scholarly journals Anti-Hepatitis A Virus Immunoglobulin M Antibodies in Urine Samples for Rapid Diagnosis of Outbreaks

2003 ◽  
Vol 10 (3) ◽  
pp. 492-494 ◽  
Author(s):  
Licel de los Angeles Rodríguez Lay ◽  
Osmany Larralde Díaz ◽  
Raiza Martínez Casanueva ◽  
Aidonis Gutiérrez Moreno
Keyword(s):  
Hepatitis A ◽  
Hepatitis A Virus ◽  
Rapid Diagnosis ◽  
Immunoglobulin M ◽  
Urine Samples ◽  
Test Results ◽  
Serum Samples ◽  
Igm Antibodies ◽  
Urine And Serum

ABSTRACT The main goal of this study was to test the feasibility of using urine for diagnosing hepatitis A virus (HAV) infections. A correlation of 90.78% between the test results of urine and serum samples was obtained. Four outbreaks of hepatitis A were confirmed by testing only urine samples. The levels of anti-HAV immunoglobulin M (IgM) antibodies in urine samples remained stable during 6 months of storage at −70°C but decreased when the samples were stored at 4°C. The results of tests of samples obtained 2 and 6 months after infection suggested that IgM levels decline more rapidly in urine than in serum.

Seroepidemiology of TORCH Infections among 1.7 Million Women of Childbearing Age in Rural China: A Population-Based Cross-Sectional Study

2021 ◽  
Author(s):  
Xiaosong Qin ◽  
Shikun Zhang ◽  
Hongbo Liu ◽  
Guixue Cheng ◽  
Yong Liu ◽  
...  
Keyword(s):  
Rural Women ◽  
Rural China ◽  
Eastern Region ◽  
Prevalence Rates ◽  
Women Of Childbearing Age ◽  
Childbearing Age ◽  
Recent Infection ◽  
Cross Sectional ◽  
Infected People ◽  
Igm Antibodies

We conducted a national seroepidemiological study of the TORCH (Toxoplasma gondii [TOX], Rubella [RV], Cytomegalovirus [CMV], and Herpes Simplex Virus) in rural women to provided updated baseline data on TORCH prevalence. A total of 1,541,329 women of childbearing age were gathered from 2010 to 2012 in China. Of these, 858,072 women were tested positive for anti-RV IgG antibodies, 602,251 women were tested positive for anti-CMV antibodies, and 40,055 women were tested positive for anti-TOX antibodies. TORCH prevalence was highest among young adults (aged 25–34 years; P < 0.0001). A total of 69,220 women (4.49%) had received RV vaccination, of whom 49,988 (72.2%) had vaccine-acquired immunity. Of 1,541,329 women, 6,107 (0.40%) tested positive for anti-TOX IgM antibodies and 6,646 (0.43%) tested positive for anti-CMV IgM antibodies, suggesting the presence of TOX and CMV infections. TORCH markers were all more prevalent in the eastern region of China than in the central or western regions (all P < 0.0001). Prevalence rates related to all recent infection markers of TOX and CMV increased with increasing age in all regions (P < 0.0001). TORCH prevalence rates were found to be lower than previously published rates. This may be attributed to improvements in living standards and health habits in China. However, considering that the decrease in prevalence has led to an increase in the number of susceptible people, and the partial immunity caused by some pathogenic infections still leave infected people at risk of reinfection, strengthened vaccination and health education is essential to improve the quality of life of the Chinese population.

scholarly journals SEROLOGICAL MARKERS OF VIRAL, SYPHILITIC AND TOXOPLASMIC INFECTION IN CHILDREN AND TEENAGERS WITH NEPHROTIC SYNDROME: CASE SERIES FROM MATO GROSSO STATE, BRAZIL

2014 ◽  
Vol 56 (6) ◽  
pp. 499-504 ◽  
Author(s):  
Silvania França da Silva Soares ◽  
Teresinha Lermen Donatti ◽  
Francisco José Dutra Souto
Keyword(s):  
Nephrotic Syndrome ◽  
Hepatitis C ◽  
Herpes Simplex ◽  
Treponema Pallidum ◽  
Clinical Signs ◽  
Case Series ◽  
Serological Markers ◽  
Recent Infection ◽  
Mato Grosso ◽  
Igm Antibodies

Some infections can be the cause of secondary nephrotic syndrome. The aim of this study was to describe the experience of a Renal Disease Reference Clinic from Central Brazil, in which serological markers of some infectious agents are systematically screened in children with nephrotic syndrome. Data were obtained from the assessment of medical files of all children under fifteen years of age, who matched nephrotic syndrome criteria. Subjects were tested for IgG and IgM antibodies against T. gondii and cytomegalovirus; antibodies against Herpes simplex, hepatitis C virus and HIV; and surface antigen (HBsAg) of hepatitis B virus. The VDRL test was also performed. 169 cases were studied. The median age on the first visit was 44 months and 103 (60.9%) patients were male. Anti-CMV IgG and IgM were found in 70.4% and 4.1%, respectively. IgG and IgM against Toxoplasma gondii were present in 32.5% and 5.3%, respectively. Two patients were positive for HBsAg, but none showed markers for HIV, hepatitis C, or Treponema pallidum. IgG and IgM against herpes simplex virus were performed on 54 patients, of which 48.1% and 22.2% were positive. IgM antibodies in some children with clinical signs of recent infection suggest that these diseases may play a role in the genesis of nephrotic syndrome.

scholarly journals Avidity of IgG antibodies against excreted/secreted antigens of Toxoplasma gondii: immunological marker for acute recent toxoplasmosis

2008 ◽  
Vol 41 (2) ◽  
pp. 142-147 ◽  
Author(s):  
Patrícia Regina Barboza Araújo ◽  
Antonio Walter Ferreira
Keyword(s):  
Toxoplasma Gondii ◽  
High Avidity ◽  
Igg Antibodies ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Immunological Marker ◽  
Specific Reactivity ◽  
Secreted Antigens ◽  
Acquired Toxoplasmosis ◽  
Single Serum Sample

Detection of anti-toxoplasma IgM antibodies has frequently been used as a serological marker for diagnosing recently acquired toxoplasmosis. However, the persistence of these antibodies in some patients has complicated the interpretation of serological results when toxoplasmosis is suspected. The purpose of the present study was to evaluate the avidity of IgG antibodies against excreted/secreted antigens of Toxoplasma gondii by means of immunoblot, to establish a profile for acute recent infection in a single serum sample and confirm the presence of residual IgM antibodies obtained in automated assays. When we evaluated the avidity of IgG antibodies against excreted/secreted antigens of Toxoplasma gondii by means of immunoblot, we observed phase-specific reactivity, i.e. cases of acute recent toxoplasmosis presented low avidity and cases of non-acute recent toxoplasmosis presented high avidity towards the 30kDa protein fraction, which probably corresponds to the SAG-1 surface antigen. Our results suggest that the avidity of IgG antibodies against excreted/secreted antigens of Toxoplasma gondii is an important immunological marker for distinguishing between recent infections and for determining the presence of residual IgM antibodies obtained from automated assays.

scholarly journals Evaluation of the TGS TA system for the detection of anti-cytomegalovirus antibodies

2017 ◽  
Vol 32 (2) ◽  
Author(s):  
Annalisa Cianflone ◽  
Maria Teresa Manco ◽  
Olivia Arpino ◽  
Alessia Paganini ◽  
Massimo De Paschale ◽  
...  
Keyword(s):  
Fluorescent Assay ◽  
Infection Group ◽  
Past Infection ◽  
Recent Infection ◽  
Igm Antibodies ◽  
Igg Avidity ◽  
Group 3 ◽  
Group 2 ◽  
Sera Samples ◽  
Group 1

<em>Background and aims:</em> The aim of the present study was to evaluate the new Technogenetics TGS TA system for detecting anti-Cytomegalovirus IgG and IgM antibodies and IgG avidity. The TGS TA system was compared with our routinely used system, LIAISON XL, for the detection of IgG and IgM antibodies. Only in positive IgM samples, TGS TA system was compared to an enzyme linked fluorescent assay (ELFA) test (VIDAS, BioMérieux, Marcy-l’Étoile, France) and with LIAISON XL system for the IgG avidity (if possible). <br /><em>Materials and methods:</em> Three hundred sera samples from pregnant women were examined with the TGS TA system and divided in 3 groups according to IgG and IgM screening LIAISON XL tests: 102 were non-immune women (Group 1), 98 were pregnant with past infection (Group 2) and 100 were pregnant with positive or equivocal IgM (95 with positive IgG and 5 with negative IgG) (Group 3). <br /><em>Results:</em> The overall concordance of the IgG results between LIAISON XL and TGS TA was 98.3%: 97.1% in Group 1, 100% in Group 2 and 98.0% in Group 3. The overall concordance of the IgM results between LIAISON XL and TGS TA was 92.1%: 100% in Group 1, 99.0% in Group 2 and 70.1% in Group 3. In Group 3, the concordance between the results of the IgG avidity with the LIAISON XL and TGS TA tests was 87.4%. Comparing the clinical diagnosis obtained with our protocol and that of the TGS TA system, the overall concordance was 94.3%: 97.1% in Group 1, 99.0% in Group 2 and 87.0% in Group 3. <br /><em>Conclusions</em>: In conclusion, the overall clinical concordance between the LIAISON XL/VIDAS protocol and the TGS TA system is excellent. TGA TA system shows to be a valuable tool able to clearly identify non-specific subjects, those with a non-recent infection and classify as either recent or past infection half of the subjects with undetermined infection with our protocol.

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