Dispersive viscosurgical devices demonstrate greater efficacy in protecting corneal endothelium in vitro

ObjectiveDuring phacoemulsification, the corneal endothelium is protected by an ophthalmic viscosurgical device (OVD). In this in vitro study, we assessed six different OVDs for their effectiveness in protecting the corneal endothelium.Methods and analysisPhacoemulsification was performed in cadaver eyes of young pigs. Five syringe units of six different OVDs were tested (Healon EndoCoat, Viscoat, Methylvisc, Healon, Healon GV, ProVisc). After surgery, the area of endothelium coated with OVD was determined in relation to the total endothelial surface. Additionally, an endothelial cell count was obtained. As a control, an endothelial cell count was obtained from freshly trephined corneas. Statistical analysis was performed using the Mann-Whitney U test and the Spearman correlation.ResultsThe least postoperative endothelial coating and cell count were observed in the cohesive OVDs while the dispersive OVDs showed statistically significant higher values. Healon EndoCoat and Viscoat yielded a coating area of 86 (85–92)% and 85 (85-90)%, respectively. Endothelial cell count was highest in the two dispersive groups with 4065 (3928–4088) cells/mm2 (Methylvisc) and 4032 (4015–4115) cells/mm2 (Viscoat). Endothelial coating area and endothelial cell count correlated statistically significantly.ConclusionDispersive OVDs from this study showed greater adherence to the endothelial surface than the cohesive ones. Furthermore, postoperative endothelial cell counts of corneas treated with dispersive OVDs were higher than of corneas treated with cohesive OVDs. Our in vitro results suggest that dispersive OVDs protect the corneal endothelium better during phacoemulsification than cohesive OVDs.

Download Full-text

Comparison between the protective effect of Ophthalmic Viscoelastics on the corneal endothelium in phacoemulsification

QJM ◽

10.1093/qjmed/hcaa058.028 ◽

2020 ◽

Vol 113 (Supplement_1) ◽

Author(s):

S M A Saleh ◽

R M F Elghazawy ◽

A I Elawamry ◽

R G Zaki

Keyword(s):

Visual Acuity ◽

Endothelial Cell ◽

Protective Effect ◽

Cell Count ◽

Corneal Endothelium ◽

Hydroxypropyl Methylcellulose ◽

Methyl Cellulose ◽

Endothelial Cell Count ◽

Significant Difference ◽

Group 2

Abstract Background Phacoemulsification is one of the most common surgical interventions done worldwide; the ultrasound power used to emulsify and remove the cataractous lens is hazardous to corneal endothelium and can lead to permenant damage. Thats why ophthalmic viscoelastics have been developed and used to protect the corneal endothelium and ensure safety of intraocualr surgeries. Objective To study the effect of different concentrations of methyl cellulose on the corneal endothelium during phacoemulsification using specular microscopy. Patients and Methods Sixty patients with Immature Senile Cataract (IMSC) are equally distributed into two groups according to the concentration of Hydroxypropyl Methylcellulose (HPMC) used during phacoemulsification. In the first group HPMC 2% was used and in the second group HPMC 2.4% was used. Preoperative and one month postoperative examination was done including visual acuity testing, slit lamp examination using Topcon SL-3C, intraocular pressure measurement using Goldmann Applanation Tonometer (GAT), fundus examination, corneal edndothelium assessment by Tomey Specular Microscope EM-3000. Results Preoperatively, no significant difference was observed in age, sex, visual acuity, introcular pressure and endothelial cell count among the two groups. Postoperatively, the two groups had a significant decrease in endothelial cell count, but the decrease was less in group 2 using Methyl cellulose 2.4% (13.494%) than in group 1 using Methyl cellulose 2% (14.515%).There was an equal and significant increase in visual acuity in the two groups. Discussion Many studies have been done to compare the efficacy of different viscoelastics (OVDs) in the protection of corneal endothelium during phacoemulsification. In our study we compared the protective effect of two different concentrations of HydroxyPropyl MethylCellulose (2% &2.4%) on the corneal endothelium during phacoemulsification. We compared the demographic data as regarding age and sex of the patients in the two groups, also we compared the preoperative endothelial cell count in the two groups using Specualr Microscopy and the result showed no significant difference between the 2 groups. The endothelial cell loss was comparable in the 2 groups, in group 1 it was 362.267 ± 52.020 (14.515 % ± 1.458) and in group 2 it was 335.667 ± 21.170 (13.494% ±0.667) with slightly better protection in the second group. So these results shows that HPMC 2.4% gives better protection than HPMC 2% on corneal endothelium during phacoemulsification on terms of the postoperative endothelial cell count and the endothelial loss after phacoemulsification. Many studies have been done to compare the efficacy and the protective effect of different viscoelastics during phacoemulsification for example the studies done by Miller et al, Maar et al, Vajpayee et al and Storr-Paulsen et al, these studies showed results similar to our study as regards the protective effect of methyl cellulose with its different concentrations on the corneal endothelium during phacoemulsification, so both concentrations (2% and 2.4%) can be used safely and effectively in phacoemulsification to decrease the endothelial loss and ensure patients safety. Conclusion Methyl cellulose 2.4% and Methyl cellulose 2% were comparable in their ability to protect the corneal endothelium during phacoemulsification, in general both viscoelastics can be efficiently and safely used in performing phacoemulsification.

Download Full-text

Corneal Endothelial Change after Phacoemulsification

Journal of Enam Medical College ◽

10.3329/jemc.v6i3.29679 ◽

2016 ◽

Vol 6 (3) ◽

pp. 134-39

Author(s):

Md Abdus Salam Siddique

Keyword(s):

Endothelial Cell ◽

Cell Count ◽

Cell Loss ◽

Modern Method ◽

Endothelial Cell Count ◽

Cell Counts ◽

Before And After ◽

The Mean ◽

Specular Microscope

Background: Phacoemulsification is the modern method of sight restoring surgery for cataract and it is becoming popular day by day. We planned this study to find out any significant cell loss during this procedure leading to corneal endothelial decompression.Objective: To compare the preoperative and postoperative cell counts after phacoemulsification surgery.Materials and Methods: This prospective study was carried out at the National Institute of Ophthalmology and Hospital, Dhaka from July to September, 2008 to see the corneal endothelial change after phacoemulsification. A total of 60 cases with lental opacity, who underwent phacoemulsification surgery, were studied with specular microscope. The endothelial cell count was measured with specular microscope before and after cataract surgery. Postoperative cases were followed up to one and half months after surgery. A single group of patients were studied and their preoperative and postoperative values were compared. The inclusion and exclusion criteria were maintained properly.Results: The mean preoperative endothelial cell count was 2745.35 ± 395.27 (SD) per mm2 which gradually decreased to 2545.23 ± 348.83 (SD) per mm2 at the end of one and half months after surgery; mean cell loss was 200.12 ± 46.44 (7.29%).Conclusion: This study reveals that a moderate loss of endothelial cell after phacoemulsification does not hamper the corneal transparency and visual function as depicted from visual acuity records in follow-up periods, provided the endothelium is healthy and the number is above the threshold limit.J Enam Med Col 2016; 6(3): 134-139

Download Full-text

Polymegathism, Pleomorphism, and Endothelial Cell Count after Selective Laser Trabeculoplasty

Biology and Medicine ◽

10.4172/0974-8369.1000343 ◽

2016 ◽

Vol 08 (07) ◽

Cited By ~ 2

Author(s):

N Kurysheva ◽

E Shatalova

Keyword(s):

Endothelial Cell ◽

Cell Count ◽

Selective Laser Trabeculoplasty ◽

Endothelial Cell Count ◽

Laser Trabeculoplasty

Download Full-text

Endothelial Cell Migration was Impaired by Irradiation-Induced Inhibition of SHP-2 in Radiotherapy: an In vitro Study

Journal of Radiation Research ◽

10.1269/jrr.10071 ◽

2011 ◽

Vol 52 (3) ◽

pp. 320-328 ◽

Cited By ~ 3

Author(s):

Xiangpeng ZHENG ◽

Sumathy MOHAN ◽

Randal A. OTTO ◽

Mohan NATARAJAN

Keyword(s):

Cell Migration ◽

Endothelial Cell ◽

In Vitro Study ◽

Vitro Study ◽

Endothelial Cell Migration

Download Full-text

Circulating endothelial cell count: a reliable marker of endothelial damage in patients undergoing hematopoietic stem cell transplantation

Bone Marrow Transplantation ◽

10.1038/bmt.2017.194 ◽

2017 ◽

Vol 52 (12) ◽

pp. 1637-1642 ◽

Cited By ~ 11

Author(s):

C Almici ◽

C Skert ◽

B Bruno ◽

A Bianchetti ◽

R Verardi ◽

...

Keyword(s):

Stem Cell ◽

Endothelial Cell ◽

Hematopoietic Stem Cell Transplantation ◽

Stem Cell Transplantation ◽

Cell Count ◽

Cell Transplantation ◽

Endothelial Damage ◽

Hematopoietic Stem ◽

Endothelial Cell Count ◽

Reliable Marker

Download Full-text

A quantitative in vitro study of fibroblast and endothelial cell migration in response to serum and wound fluid

Journal of Surgical Research ◽

10.1016/s0022-4804(83)80011-0 ◽

1983 ◽

Vol 35 (3) ◽

pp. 249-258 ◽

Cited By ~ 19

Author(s):

S.U. Orredson ◽

D.R. Knighton ◽

H. Scheuenstuhl ◽

T.K. Hunt

Keyword(s):

Cell Migration ◽

Endothelial Cell ◽

In Vitro Study ◽

Vitro Study ◽

Endothelial Cell Migration ◽

Wound Fluid

Download Full-text

Potential Use of Silica Nanoparticles for the Microbial Stabilisation of Wine: An In Vitro Study Using Oenococcus oeni as a Model

Foods ◽

10.3390/foods9091338 ◽

2020 ◽

Vol 9 (9) ◽

pp. 1338

Author(s):

Kamila Pachnowska ◽

Krzysztof Cendrowski ◽

Xymena Stachurska ◽

Paweł Nawrotek ◽

Adrian Augustyniak ◽

...

Keyword(s):

In Vitro Study ◽

Oenococcus Oeni ◽

Chemical Oxidation ◽

Alternative Methods ◽

Bacterial Cells ◽

Silica Nanospheres ◽

Cell Counts ◽

Transmission Electron ◽

Phosphate Buffered Saline

The emerging trend towards the reduction of SO2 in winemaking has created a need to look for alternative methods to ensure the protection of wine against the growth of undesired species of microorganisms and to safely remove wine microorganisms. This study describes the possible application of silica nanospheres as a wine stabilisation agent, with Oenococcus oeni (DSM7008) as a model strain. The experiment was conducted firstly on model solutions of phosphate-buffered saline and 1% glucose. Their neutralising effect was tested under stirring with the addition of SiO2 (0.1, 0.25, and 0.5 mg/mL). Overall, the highest concentration of nanospheres under continuous stirring resulted in the greatest decrease in cell counts. Transmission electron microscope (TEM) and scanning electron microscopy (SEM) analyses showed extensive damage to the bacterial cells after stirring with silica nanomaterials. Then, the neutralising effect of 0.5 mg/mL SiO2 was tested in young red wine under stirring, where cell counts were reduced by over 50%. The obtained results suggest that silica nanospheres can serve as an alternative way to reduce or substitute the use of sulphur dioxide in the microbial stabilisation of wine. In addition, further aspects of following investigations should focus on the protection against enzymatic and chemical oxidation of wine.

Download Full-text