132 Targeted Resuscitation Using Echocardiography in a Military Ebola Virus Disease Treatment Unit

Abstract Background Rates of bacteremia in Ebola virus disease (EVD) are not currently known. Given the potential for secondary bacterial infection during acute EVD, current treatment guidelines recommend empiric use of broad-spectrum antibiotics. We sought to determine rates of bacteremia among patients evaluated for EVD at the ELWA-3 Ebola Treatment Unit in Monrovia, Liberia during the 2013–16 West Africa epidemic. Methods Deidentified blood samples and matched clinical data from 235 Ebola virus (EBOV)-positive patients and 102 EBOV-negative patients were evaluated under a University of Liberia Pacific Institute for Research and Evaluation IRB-approved protocol. 0.2 mL aliquots of frozen whole blood samples collected at triage, prior to the administration of antibiotics, were inoculated into BD BACTEC Peds Plus bottles and incubated under aerobic conditions in a BD BACTEC FX40 for 5 days in the National Institute of Allergy and Infectious Disease Biosafety Level 4 laboratory in Hamilton, MT. Positive samples were sub-cultured on nonselective sheep blood agar and chocolate agar and pure colonies were selected for identification by 16S sequencing and by matrix assisted laser desorption ionization time-of-flight mass spectrometry. Results No difference in rates of bacteremia was detected among EBOV-positive vs. EBOV-negative patients – 3.8% and 3.9%, respectively. Predominant isolates included Staphylococcus epidermidis and other coagulase-negative staphylococci, thought consistent with contaminants. Pathogenic species included Staphylococcus aureus and possibly Paenibacillus spp. Conclusion These data suggest that bacteremia does not commonly complicate EVD. However, as both prolonged sample storage and low culture volume may negatively affect sensitivity, additional molecular analyses are needed to support this conclusion. The Intramural Research Program of the National Institutes of Health supported this work. Disclosures All authors: No reported disclosures.

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A Systematic Review of Computational Drug Discovery, Development, and Repurposing for Ebola Virus Disease Treatment

Molecules ◽

10.3390/molecules22101777 ◽

2017 ◽

Vol 22 (10) ◽

pp. 1777 ◽

Cited By ~ 16

Author(s):

James Schuler ◽

Matthew Hudson ◽

Diane Schwartz ◽

Ram Samudrala

Keyword(s):

Systematic Review ◽

Drug Discovery ◽

Ebola Virus ◽

Virus Disease ◽

Ebola Virus Disease ◽

Disease Treatment ◽

Computational Drug Discovery

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Post-Ebola Syndrome among Ebola Virus Disease Survivors in Montserrado County, Liberia 2016

BioMed Research International ◽

10.1155/2018/1909410 ◽

2018 ◽

Vol 2018 ◽

pp. 1-8 ◽

Cited By ~ 21

Author(s):

Himiede W. Wilson ◽

Maame Amo-Addae ◽

Ernest Kenu ◽

Olayinka Stephen Ilesanmi ◽

Donne K. Ameme ◽

...

Keyword(s):

Ebola Virus ◽

Psychological Symptoms ◽

Virus Disease ◽

Clinical Care ◽

Adult Population ◽

Cross Sectional Study ◽

Ebola Virus Disease ◽

Cross Sectional ◽

Treatment Unit ◽

Health History

Introduction. An increased number of survivors have emerged from the 2014 West African Ebola Virus Disease (EVD) epidemic. Post-Ebola Syndrome (PES) is a group of physical and psychological symptoms affecting EVD survivors. This study aimed to estimate the prevalence of PES among EVD survivors in Montserrado County, Liberia. Method. A cross-sectional study design was conducted to determine the prevalence of PES, types, onset, and duration among survivors. Survivors in Montserrado County were recruited using multistage sampling methods. Quantitative data was collected using semistructured questionnaire. Variables were collected on EVD survivors demographics, pre- and post-Ebola health history. Result. Prevalence of Post-Ebola Syndrome was estimated to be 90% (242/268). PES was experienced by 67% (162/242) females. PES occurred mainly in the adult population between ages 25-34, 35% (84/242). The commonest symptoms were reported from the following systems of the human body: neurological system (eyes problem, headache, sleep disorder, and unusual tiredness) and musculoskeletal system (abdominal pain, chest pain, and joints pains). The onset of PES occurred between the first 1-12 weeks after being discharged from a treatment unit. Conclusion. Prevalence of PES is high. Clinical care for survivors should be strengthened.

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