scholarly journals P2.21 Feasibility study for the development of a rapid lateral flow point of care test for congenital syphilis

2017 ◽  
Author(s):  
John Deutsch ◽  
Yetunda F Fakile ◽  
Ellen Kersh ◽  
Javan Esfandiari ◽  
Ardianne Cruz
Keyword(s):  
Feasibility Study ◽  
Congenital Syphilis ◽  
Point Of Care ◽  
Lateral Flow ◽  
Point Of Care Test

scholarly journals Comparative Analysis of the Euroimmun CXCL13 Enzyme-Linked Immunosorbent Assay and the ReaScan Lateral Flow Immunoassay for Diagnosis of Lyme Neuroborreliosis

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Katharina Ziegler ◽  
Anca Rath ◽  
Christoph Schoerner ◽  
Renate Meyer ◽  
Thomas Bertsch ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Roc Curve ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lyme Neuroborreliosis ◽  
Diagnostic Tools ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
European Federation ◽  
Point Of Care Test

ABSTRACT Diagnosis of Lyme neuroborreliosis (LNB) is challenging, as long as Borrelia-specific intrathecal antibodies are not yet detectable. The chemokine CXCL13 is elevated in the cerebrospinal fluid (CSF) of LNB patients. Here, we compared the performances of the Euroimmun CXCL13 enzyme-linked immunosorbent assay (CXCL13 ELISA) and the ReaScan CXCL13 lateral flow immunoassay (CXCL13 LFA), a rapid point-of-care test, to support the diagnosis of LNB. In a dual-center case-control study, CSF samples from 90 patients (34 with definite LNB, 10 with possible LNB, and 46 with other central nervous system [CNS] diseases [non-LNB group]) were analyzed with the CXCL13 ELISA and the CXCL13 LFA. Classification of patients followed the European Federation of Neurological Societies (EFNS) guidelines on LNB. The CXCL13 ELISA detected elevated CXCL13 levels in all patients with definite LNB (median, 1,409 pg/ml) compared to the non-LNB controls (median, 20.7 pg/ml; P < 0.0001), with a sensitivity of 100% and a specificity of 84.8% (cutoff value, 78.6 pg/ml; area under the receiver operating characteristic [ROC] curve, 0.93). Similarly, the CXCL13 LFA yielded elevated CXCL13 levels in 31 patients with definite LNB (median arbitrary value, 223.5) compared to the non-LNB control patients (median arbitrary value, 0; P < 0.0001) and had a sensitivity and specificity of 91.2% and 93.5%, respectively (cutoff arbitrary value, 22.5; area under the ROC curve, 0.94). The correlation between the CXCL13 levels obtained by ELISA and LFA was strong (Spearman correlation coefficient r = 0.89; P < 0.0001). The CXCL13 ELISA and the CXCL13 LFA are comparable diagnostic tools for the detection of CXCL13 in the CSF of patients with definite LNB. The advantage of the CXCL13 LFA is the shorter time to result.

scholarly journals Method for the elucidation of LAMP products captured on lateral flow strips in a point of care test for HPV 16

2020 ◽  
Vol 412 (24) ◽  
pp. 6199-6209
Author(s):  
Lena Landaverde ◽  
Winnie Wong ◽  
Gabriela Hernandez ◽  
Andy Fan ◽  
Catherine Klapperich
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Hpv 16 ◽  
Point Of Care Test ◽  
Lateral Flow Strips

scholarly journals A new lateral flow assay to detect sIL-2R during T-cell mediated rejection after kidney transplantation

The Analyst ◽  
2021 ◽  
Author(s):  
Lisa K. Seiler ◽  
Rebecca Jonczyk ◽  
Patrick Lindner ◽  
Ncog Linh Phung ◽  
Christine S. Falk ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
T Cell ◽  
Point Of Care ◽  
High Specificity ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Point Of Care Test ◽  
Specificity And Sensitivity ◽  
Kidney Rejection ◽  
Acute Kidney Rejection

In this work a novel point of care test to detect sIL-2R during acute kidney rejection with high specificity and sensitivity was developed.

Diagnostic ability of salivary matrix metalloproteinase‐9 lateral flow test point‐of‐care test for periodontitis

2020 ◽  
Vol 47 (11) ◽  
pp. 1354-1361
Author(s):  
Hyun‐Duck Kim ◽  
Chang‐Soo Lee ◽  
Hyun‐Jae Cho ◽  
Sumin Jeon ◽  
Young‐Nim Choi ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Point Of Care ◽  
Test Point ◽  
Matrix Metalloproteinase 9 ◽  
Lateral Flow ◽  
Diagnostic Ability ◽  
Point Of Care Test ◽  
Flow Test ◽  
Lateral Flow Test ◽  
Metalloproteinase 9

A new point-of-care test for the diagnosis of infectious diseases based on multiplex lateral flow immunoassays

The Analyst ◽  
2019 ◽  
Vol 144 (8) ◽  
pp. 2460-2466 ◽  
Author(s):  
Hanbi Kim ◽  
Doo-Ryeon Chung ◽  
Minhee Kang
Keyword(s):  
Infectious Diseases ◽  
Point Of Care ◽  
Lateral Flow ◽  
Point Of Care Test

Multiplex lateral flow immunoassays for outstanding POCT of infectious diseases.

scholarly journals Evaluation of a Rapid Lateral Flow Point-of-Care Test for Detection of Cryptosporidium

2016 ◽  
Vol 95 (4) ◽  
pp. 840-841 ◽  
Author(s):  
Molly E. Fleece ◽  
Jack Heptinstall ◽  
Shaila S. Khan ◽  
Mamum Kabir ◽  
Joel Herbein ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Point Of Care Test

scholarly journals Upconverting phosphor technology-based lateral flow assay for the rapid and sensitive detection of anti-Trichinella spiralis IgG antibodies in pig serum

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Jian Li ◽  
Jing Ding ◽  
Xiao-Lei Liu ◽  
Bin Tang ◽  
Xue Bai ◽  
...  
Keyword(s):  
Trichinella Spiralis ◽  
Point Of Care ◽  
Cross Reactivity ◽  
Lateral Flow ◽  
Sensitive Detection ◽  
Parasitic Infections ◽  
Igg Antibodies ◽  
Point Of Care Test ◽  
Rabbit Igg ◽  
Pig Serum

Abstract Background Trichinella spiralis is a zoonotic food-borne parasite. A disease caused by infection with T. spiralis is called trichinellosis in humans. It is important to investigate the epidemic situation and the surveillance of herds and then prevent infection in humans. Therefore, this study is to develop a rapid and sensitive diagnostic method for on-site test in domestic and wild animals. Methods Upconverting phosphor nanoparticles (UCNPs), an excellent optical label, were conjugated with the excretory-secretory (ES) antigens from T. spiralis muscle larvae (ML) or goat anti-rabbit IgG, and a lateral flow (LF) assay based on these probes (UCNPs-ES/goat anti-rabbit IgG) was developed for the rapid and sensitive detection of anti-T. spiralis IgG antibodies in pig serum. The assay is named the UPT-LF-ES assay. In addition, the probes were characterized, and the assay was optimized. A cut-off threshold of the assay was also identified by using 169 known negative pig samples. Performance of the assay to T. spiralis with different infective numbers, cross-reactivity with other parasitic infections, the single-blinded experiment, and coincidence were evaluated with the assay. Results The UPT-LF-ES assay was successfully constructed and optimized based on the probes of UCNPs-ES/goat anti-rabbit IgG. In the pigs infected with 100, 1000, and 10,000 ML, positive results were first presented at 35 days post-infection (dpi), 30 dpi, and 25 dpi, respectively. The assay had no cross-reaction with other parasitic infections. A single-blinded experiment indicated that the sensitivity and specificity of the UPT-LF-ES assay were 100% and 100%, respectively, the area under the receiver operating characteristic (ROC) curve was 1.000. In addition, the value detected by the UPT-LF-ES assay was significantly different between positive and negative samples. Moreover, compared with the “gold standard” magnetic stirrer method, the coincidence rate of the UPT-LF-ES assay was 87.27%, and the kappa (K) coefficient was 0.7454, showing a substantial agreement. Conclusions The UPT-LF-ES assay is a useful point-of-care test (POCT) with T. spiralis in the detection of pig, which contributes to preventing human trichinellosis. Graphical Abstract

scholarly journals Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

2011 ◽  
Vol 5 (11) ◽  
pp. 786-791 ◽  
Author(s):  
Ayman Marei ◽  
Ghada Boghdadi ◽  
Nahla Abdel-Hamed ◽  
Rasha Hessin ◽  
Theresia Abdoel ◽  
...  
Keyword(s):  
Point Of Care ◽  
Public Health Problem ◽  
Laboratory Diagnosis ◽  
Diagnostic Value ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Human Brucellosis ◽  
Major Public Health Problem ◽  
Serum Samples ◽  
Point Of Care Test

Introduction: Brucellosis is a major public health problem in Egypt. The Brucella IgM/IgG lateral flow assay was developed as a point-of-care test for the diagnosis of human brucellosis. The aim of this study was to assess the diagnostic value of the lateral flow assay for use in Egypt. Methodology: Fifty samples of patients who presented with clinical suspicion of brucellosis over a one-year period were collected.  All samples were subjected to the Brucella IgM/IgG lateral flow assay, serum agglutination test (SAT), rose bengal RB Test (RB), 2- mercapteoethanol (2-ME), culture and PCR. SAT, 2- ME, culture and PCR were retested after the end of the treatment. Results: Culture and SAT confirmed the diagnosis of brucellosis in twenty patients.  While 90% of the samples were positive by SAT, only 30% and 85% were positive by culture and PCR respectively. The sensitivity of the lateral flow assay calculated for the Brucella IgM/IgG was 95% and specificity was 97%. Conclusion: These data show that the lateral flow assay is more suitable for diagnosis of brucellosis in Egypt than culture and SAT.  Application of the PCR on serum samples collected during follow-up revealed that the DNA of the pathogen was yet not completely cleared almost 60 days after the start of treatment with doxycycline and ciprofloxacin.

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