scholarly journals Laboratory diagnosis of human brucellosis in Egypt and persistence of the pathogen following treatment

2011 ◽  
Vol 5 (11) ◽  
pp. 786-791 ◽  
Author(s):  
Ayman Marei ◽  
Ghada Boghdadi ◽  
Nahla Abdel-Hamed ◽  
Rasha Hessin ◽  
Theresia Abdoel ◽  
...  
Keyword(s):  
Point Of Care ◽  
Public Health Problem ◽  
Laboratory Diagnosis ◽  
Diagnostic Value ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Human Brucellosis ◽  
Major Public Health Problem ◽  
Serum Samples ◽  
Point Of Care Test

Introduction: Brucellosis is a major public health problem in Egypt. The Brucella IgM/IgG lateral flow assay was developed as a point-of-care test for the diagnosis of human brucellosis. The aim of this study was to assess the diagnostic value of the lateral flow assay for use in Egypt. Methodology: Fifty samples of patients who presented with clinical suspicion of brucellosis over a one-year period were collected.  All samples were subjected to the Brucella IgM/IgG lateral flow assay, serum agglutination test (SAT), rose bengal RB Test (RB), 2- mercapteoethanol (2-ME), culture and PCR. SAT, 2- ME, culture and PCR were retested after the end of the treatment. Results: Culture and SAT confirmed the diagnosis of brucellosis in twenty patients.  While 90% of the samples were positive by SAT, only 30% and 85% were positive by culture and PCR respectively. The sensitivity of the lateral flow assay calculated for the Brucella IgM/IgG was 95% and specificity was 97%. Conclusion: These data show that the lateral flow assay is more suitable for diagnosis of brucellosis in Egypt than culture and SAT.  Application of the PCR on serum samples collected during follow-up revealed that the DNA of the pathogen was yet not completely cleared almost 60 days after the start of treatment with doxycycline and ciprofloxacin.

scholarly journals A new lateral flow assay to detect sIL-2R during T-cell mediated rejection after kidney transplantation

The Analyst ◽  
2021 ◽  
Author(s):  
Lisa K. Seiler ◽  
Rebecca Jonczyk ◽  
Patrick Lindner ◽  
Ncog Linh Phung ◽  
Christine S. Falk ◽  
...  
Keyword(s):  
Kidney Transplantation ◽  
T Cell ◽  
Point Of Care ◽  
High Specificity ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Point Of Care Test ◽  
Specificity And Sensitivity ◽  
Kidney Rejection ◽  
Acute Kidney Rejection

In this work a novel point of care test to detect sIL-2R during acute kidney rejection with high specificity and sensitivity was developed.

scholarly journals Comparative Study of the Accuracy of Different Techniques for the Laboratory Diagnosis of Schistosomiasis Mansoni in Areas of Low Endemicity in Barra Mansa City, Rio de Janeiro State, Brazil

2015 ◽  
Vol 2015 ◽  
pp. 1-16 ◽  
Author(s):  
Maria Cristina Carvalho Espírito-Santo ◽  
Mónica Viviana Alvarado-Mora ◽  
Pedro Luiz Silva Pinto ◽  
Maria Carmen Arroyo Sanchez ◽  
Emmanuel Dias-Neto ◽  
...  
Keyword(s):  
Public Health Problem ◽  
Laboratory Diagnosis ◽  
Epidemiological Survey ◽  
Enzyme Linked Immunosorbent Assay ◽  
Major Public Health Problem ◽  
Serum Samples ◽  
Current Standard ◽  
Helminth Infections ◽  
Indirect Immunofluorescence Technique ◽  
Precipitin Test

Schistosomiasis constitutes a major public health problem, with an estimated 200 million people infected worldwide. Many areas of Brazil show low endemicity of schistosomiasis, and the current standard parasitological techniques are not sufficiently sensitive to detect the low-level helminth infections common in areas of low endemicity (ALEs). This study compared the Kato-Katz (KK); Hoffman, Pons, and Janer (HH); enzyme-linked immunosorbent assay- (ELISA-) IgG and ELISA-IgM; indirect immunofluorescence technique (IFT-IgM); and qPCR techniques for schistosomiasis detection in serum and fecal samples, using the circumoval precipitin test (COPT) as reference. An epidemiological survey was conducted in a randomized sample of residents from five neighborhoods of Barra Mansa, RJ, with 610 fecal and 612 serum samples. ELISA-IgM (21.4%) showed the highest positivity and HH and KK techniques were the least sensitive (0.8%). All techniques except qPCR-serum showed high accuracy (82–95.5%), differed significantly from COPT in positivityP<0.05, and showed poor agreement with COPT. Medium agreement was seen with ELISA-IgG (Kappa = 0.377) and IFA (Kappa = 0.347). Parasitological techniques showed much lower positivity rates than those by other techniques. We suggest the possibility of using a combination of laboratory tools for the diagnosis of schistosomiasis in ALEs.

scholarly journals BRUCELLOSIS

2018 ◽  
Vol 25 (12) ◽  
pp. 1828-1832
Author(s):  
Ali Faraz ◽  
Syed Yousaf Kazmi ◽  
Muhammad Asad Farhan ◽  
Usama Bin Ghaffar ◽  
Sajid Hussain ◽  
...  
Keyword(s):  
Saudi Arabia ◽  
Public Health Problem ◽  
Risk Groups ◽  
Agglutination Test ◽  
Human Brucellosis ◽  
Major Public Health Problem ◽  
Seroprevalence Rate ◽  
Serum Samples ◽  
Cross Sectional ◽  
Nomadic Life

Background: Human brucellosis is a zoonotic infectious disease transmitted from domesticated animals to humans. It remains a major public health problem in Saudi Arabia where 7% of the population still maintains a nomadic life style with domestication of animals. Objectives: This study aims to discover the prevalence of brucellosis and Brucella among patients attending a district government hospital in Majmaah, kingdom of Saudi Arabia. Period: 2 years (from 1st February 2016 to 31st January 2018). Design: Cross sectional descriptive. Settings: King Khalid Hospital, Al Majmaah, Saudi Arabia. Materials (Patients) & Methods: Total 1098 serum samples from the patients with clinical suspicion of brucellosis were screened with rapid slide agglutination test (crescent diagnostics Jeddah) for Brucella antibodies and later confirmed by Serum Tube Agglutination Test. Main Outcome Measures: The result of our study showed that the prevalence of brucellosis among patients attending our study is 9.1%. Results: 100 individuals were found to be seropositive with titers ≥1:160. The result of our study showed that the prevalence of brucellosis among patients attending our study is 9.1%The Majority of the patients presented to the medical OPD and orthopedic unit of the hospital. The prevalence of disease in males was found to be higher as compared to females in the current studied group. The age group 21- 40 years, was found to be far more susceptible to this infection. Majority presented with fever and musculoskeletal complaints. The total seroprevalence rate calculated for the patients attending our hospital is 26.50%. Conclusion: Frequent serological surveillance should be carried out in areas that are endemic. Screening of risk groups, imported animals and household members of active brucellosis must be undertaken. This is a crucial epidemiological move allowing for timely diagnosis and control of disease. Limitations: Our prevalence rate represents a specific segment of the population (i.e., those attending the hospital) and not thegeneral population. 

scholarly journals Evaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosis

2020 ◽  
Author(s):  
Richard Kwizera ◽  
Denis Omali ◽  
Kiiza Tadeo ◽  
John Kasibante ◽  
Morris K. Rutakingirwa ◽  
...  
Keyword(s):  
Predictive Value ◽  
Diagnostic Performance ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Sub Saharan Africa ◽  
Serum Samples ◽  
Cryptococcal Antigen ◽  
Asymptomatic Patients ◽  
Sub Saharan

Background: Cryptococcal meningitis is a leading cause of meningitis in sub-Saharan Africa. Given the need for rapid point of care testing, we evaluated the diagnostic performance of the Dynamiker cryptococcal antigen (CrAg) lateral flow assay (LFA). Methods: We assessed the diagnostic performance of the Dynamiker CrAg-LFA compared to the IMMY CrAg-LFA as the reference standard. We tested 150 serum, 115 plasma, 100 cerebrospinal fluid (CSF) samples from HIV patients with symptomatic meningitis and 113 serum samples from patients with suspected asymptomatic cryptococcal antigenemia. Results: Compared to the IMMY CrAg-LFA, sensitivity of Dynamiker CrAg-LFA was 98% in serum, 100% in plasma, 100% in CSF from symptomatic patients and 96% in serum from asymptomatic patients. Specificity was 66% in serum, 61% in plasma, 91% in CSF from symptomatic patients, and 86% in serum from asymptomatic patients. The positive predictive value was 85% in serum, 82% in plasma, 96% in CSF from symptomatic patients, and 69% in serum from asymptomatic patients. The negative predictive value was 94% in serum, 100% in plasma, 100% in CSF from symptomatic patients, and 99% in serum from asymptomatic patients. The inter-assay reproducibility was 100% across the four sample types with no observed discordant results when Dynamiker CrAg-LFA was tested in duplicate. However, a high number of false positives were observed on serum of symptomatic patients (11%), serum of asymptomatic patients (11%) and plasma of symptomatic patients (14%). Conclusion: The Dynamiker CrAg-LFA had excellent sensitivity but poor specificity, particularly when tested on serum and plasma.

Evaluation of a commercially available, point-of-care Coccidioides antibody lateral flow assay to aid in rapid diagnosis of coccidioidomycosis in dogs

2019 ◽  
Vol 58 (3) ◽  
pp. 328-332 ◽  
Author(s):  
Sallianne Schlacks ◽  
Polina Vishkautsan ◽  
Christine Butkiewicz ◽  
Lisa Shubitz
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Reference Laboratory ◽  
Serum Samples ◽  
Percent Agreement ◽  
Life Threatening Illness ◽  
Life Threatening ◽  
Paired Serum ◽  
High Level

Abstract Coccidioidomycosis in dogs can range from mild respiratory disease or vague, chronic malaise to acute, severe life-threatening illness. The diagnosis of coccidioidomycosis in dogs is based on clinical presentation and serology. Spherule identification is not typical because of low numbers of organisms in specimens, and the invasive nature of sampling tissues and lungs. Conventional serological assays require samples to be submitted to a reference laboratory and results take several days to one week. The sōna Coccidioides Antibody Lateral Flow Assay (LFA) (IMMY Diagnostics) is a rapid, bench-side test used for detection of Coccidioides antibodies that is available and FDA-cleared for use in humans but has not been evaluated in dogs. The goal of this study was to compare the LFA to conventional agar gel immunodiffusion (AGID). Paired serum samples were collected for screening by the LFA and submitted to a commercial reference laboratory for AGID screen and titer. Of 56 paired serum samples analyzed, 30 were positive and 26 were negative on the sōna Coccidioides antibody LFA. The overall percentage agreement plus 95% confidence interval (CI) was 87.5% (76.20–93.99). Positive percent agreement was 89.7% (73.38–96.65) and negative percent agreement was 85.2% (67.25–94.36). The kappa coefficient to assess agreement was 0.749 (95% CI, 0.576–0.923), which is interpreted as good agreement between the tests (&gt;70%). The sōna Coccidioides antibody LFA provided rapid, point-of-care results with a high level of agreement to standard AGID serology in dogs clinically suspected to have coccidioidomycosis, and may aid in diagnosis of coccidioidomycosis in dogs.

A rapid point-of-care test for dengue virus-1 based on a lateral flow assay with a near-infrared fluorescent dye

2018 ◽  
Vol 456 ◽  
pp. 23-27 ◽  
Author(s):  
Lin Chen ◽  
Huagui Wang ◽  
Tongsheng Guo ◽  
Chaohui Xiao ◽  
Licheng Liu ◽  
...  
Keyword(s):  
Dengue Virus ◽  
Near Infrared ◽  
Point Of Care ◽  
Fluorescent Dye ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Point Of Care Test

Evaluation of a lateral flow assay–based IFN-γ release assay as a point-of-care test for the diagnosis of latent tuberculosis infection

2021 ◽  
Author(s):  
Hoon Hee Lee ◽  
Dong Hwan Choi ◽  
Jeong-Ran Kim ◽  
Young Gyun Kim ◽  
Kyung-Wook Jo ◽  
...  
Keyword(s):  
Latent Tuberculosis Infection ◽  
Point Of Care ◽  
Latent Tuberculosis ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Tuberculosis Infection ◽  
Point Of Care Test ◽  
Release Assay ◽  
Ifn Γ

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM).The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

A Point of Care Lateral Flow Assay for Rapid and Colorimetric Detection of Interleukin 6 and Perspectives in Bedside Diagnostics

2020 ◽  
Author(s):  
Carla Eiras
Keyword(s):  
Interleukin 6 ◽  
Limit Of Detection ◽  
Inflammatory Diseases ◽  
Point Of Care ◽  
Colorimetric Detection ◽  
Lateral Flow ◽  
Lateral Flow Assay ◽  
Aggregation Assay ◽  
Before And After ◽  
Bedside Diagnosis

Interleukin-6 (IL-6) is a multifunctional cytokine and high bloodstream levels of which have been associated with severe inflammatory diseases, such as dengue fever, sepsis, various cancers, and visceral leishmaniasis (VL). Rapid tests for the quantification of IL-6 would be of great assistance for the bedside diagnosis and treatment of diseases such as VL. We have developed a lateral flow assay (LFA) for rapid and colorimetric IL-6 detection, consisting of anti-IL-6 antibodies conjugated to gold nanoparticles (AuNPs). The optimal concentration of anti-IL-6 used in the conjugate was determined to be 800.0 μg/mL, based on an aggregation assay using LFA. A linear relationship between IL-6 standard concentration and color intensity was observed after 20 min, with a linear range between 1.25 ng/mL and 9,000 ng/mL. The limit of detection for this method was estimated at a t0.38 ng/mL. The concentration of IL-6 in five patients with severe VL was measured using LFA, and the results were consistent with those obtained using the cytometric bead array (CBA) method. A thorough analysis of the LFA membranes’ surface morphology, before and after sample contact, was performed using atomic force microscopy (AFM). The prototype described here is still being tested and improved, but this LFA will undoubtedly be of great help in the clinical quantification of IL-6.

scholarly journals Comparative Analysis of the Euroimmun CXCL13 Enzyme-Linked Immunosorbent Assay and the ReaScan Lateral Flow Immunoassay for Diagnosis of Lyme Neuroborreliosis

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Katharina Ziegler ◽  
Anca Rath ◽  
Christoph Schoerner ◽  
Renate Meyer ◽  
Thomas Bertsch ◽  
...  
Keyword(s):  
Immunosorbent Assay ◽  
Roc Curve ◽  
Point Of Care ◽  
Lateral Flow ◽  
Lyme Neuroborreliosis ◽  
Diagnostic Tools ◽  
Lateral Flow Immunoassay ◽  
Enzyme Linked Immunosorbent Assay ◽  
European Federation ◽  
Point Of Care Test

ABSTRACT Diagnosis of Lyme neuroborreliosis (LNB) is challenging, as long as Borrelia-specific intrathecal antibodies are not yet detectable. The chemokine CXCL13 is elevated in the cerebrospinal fluid (CSF) of LNB patients. Here, we compared the performances of the Euroimmun CXCL13 enzyme-linked immunosorbent assay (CXCL13 ELISA) and the ReaScan CXCL13 lateral flow immunoassay (CXCL13 LFA), a rapid point-of-care test, to support the diagnosis of LNB. In a dual-center case-control study, CSF samples from 90 patients (34 with definite LNB, 10 with possible LNB, and 46 with other central nervous system [CNS] diseases [non-LNB group]) were analyzed with the CXCL13 ELISA and the CXCL13 LFA. Classification of patients followed the European Federation of Neurological Societies (EFNS) guidelines on LNB. The CXCL13 ELISA detected elevated CXCL13 levels in all patients with definite LNB (median, 1,409 pg/ml) compared to the non-LNB controls (median, 20.7 pg/ml; P < 0.0001), with a sensitivity of 100% and a specificity of 84.8% (cutoff value, 78.6 pg/ml; area under the receiver operating characteristic [ROC] curve, 0.93). Similarly, the CXCL13 LFA yielded elevated CXCL13 levels in 31 patients with definite LNB (median arbitrary value, 223.5) compared to the non-LNB control patients (median arbitrary value, 0; P < 0.0001) and had a sensitivity and specificity of 91.2% and 93.5%, respectively (cutoff arbitrary value, 22.5; area under the ROC curve, 0.94). The correlation between the CXCL13 levels obtained by ELISA and LFA was strong (Spearman correlation coefficient r = 0.89; P < 0.0001). The CXCL13 ELISA and the CXCL13 LFA are comparable diagnostic tools for the detection of CXCL13 in the CSF of patients with definite LNB. The advantage of the CXCL13 LFA is the shorter time to result.

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