scholarly journals Effect of pH on the motility and virulence of Treponema pallidum (Nichols) and Treponema paraluis-cuniculi in vitro under anaerobic conditions.

1980 ◽  
Vol 56 (4) ◽  
pp. 269-269
Author(s):  
S Graves ◽  
T Gotv ◽  
F Trewartha
Keyword(s):  
Treponema Pallidum ◽  
Anaerobic Conditions ◽  
Effect Of Ph

Examination of various cell culture techniques for co-incubation of virulent Treponema pallidum (Nichols I strain) under anaerobic conditions

1976 ◽  
Vol 4 (4) ◽  
pp. 360-371
Author(s):  
P L Sandok ◽  
S T Knight ◽  
H M Jenkin
Keyword(s):  
Cell Culture ◽  
Mammalian Cells ◽  
Culture Medium ◽  
Treponema Pallidum ◽  
Dark Field ◽  
Anaerobic Conditions ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Dark Field Microscopy

Treponema pallidum (Nichols virulent) was incubated with and without cells in cell culture medium reduced to -275 mV Ecal, pH 7.3, under deoxygenated conditions. Five to ten percent of the treponemes attached to cells and remained motile for at least 120 h in cell-treponeme systems of co-incubation. Virulent treponemes could be detected after 120 to 144 h in the supernatant fluids of cell-treponeme co-incubation cultures and in cell-free tubes containing medium harvested from aerobically cultivated mammalian cells. Medium supplemented with ox serum ultrafiltrate, pyruvate, and sodium thioglycolate and gas mixtures containing H2 and CO2 enhanced treponemal survival. Increases in treponemal numbers were observed using dark-field microscopy but were not substantiated using the rabbit lesion test. Continuous passage of the treponeme was not achieved in vitro.

scholarly journals P074 In vitro and in vivo efficacy of linezolid on Treponema pallidum, the syphilis agent

2021 ◽  
Author(s):  
L Giacani ◽  
A Haynes ◽  
M Vall Mayans ◽  
M Ubals Cazorla ◽  
C Nieto ◽  
...  
Keyword(s):  
Treponema Pallidum ◽  
In Vivo Efficacy

scholarly journals In Vitro Cultivation of the Syphilis Spirochete Treponema pallidum

2021 ◽  
Vol 1 (2) ◽  
Author(s):  
Diane G. Edmondson ◽  
Steven J. Norris
Keyword(s):  
Treponema Pallidum ◽  
In Vitro Cultivation

scholarly journals Effect of pH on Fermentation Characteristics and Protein Degradation by Rumen Microorganisms In Vitro

1982 ◽  
Vol 65 (8) ◽  
pp. 1457-1464 ◽  
Author(s):  
J.D. Erfle ◽  
R.J. Boila ◽  
R.M. Teather ◽  
S. Mahadevan ◽  
F.D. Sauer
Keyword(s):  
Protein Degradation ◽  
Rumen Microorganisms ◽  
Effect Of Ph

Lethal photosensitization of Porphyromonas gingivalis by their endogenous porphyrins under anaerobic conditions: An in vitro study

2013 ◽  
Vol 10 (4) ◽  
pp. 677-682 ◽  
Author(s):  
Christopher K. Hope ◽  
Jonathan A. Hindley ◽  
Zeeshan Khan ◽  
Elbert de Josselin de Jong ◽  
Susan M. Higham
Keyword(s):  
Porphyromonas Gingivalis ◽  
In Vitro Study ◽  
Vitro Study ◽  
Anaerobic Conditions

In vitro metabolism of rebaudioside B, D, and M under anaerobic conditions: Comparison with rebaudioside A

2014 ◽  
Vol 68 (2) ◽  
pp. 259-268 ◽  
Author(s):  
Sidd Purkayastha ◽  
George Pugh ◽  
Barry Lynch ◽  
Ashley Roberts ◽  
David Kwok ◽  
...  
Keyword(s):  
In Vitro Metabolism ◽  
Rebaudioside A ◽  
Anaerobic Conditions

scholarly journals Anaerobic Conditions Induce Expression of Polysaccharide Intercellular Adhesin in Staphylococcus aureus and Staphylococcus epidermidis

2001 ◽  
Vol 69 (6) ◽  
pp. 4079-4085 ◽  
Author(s):  
Sarah E. Cramton ◽  
Martina Ulrich ◽  
Friedrich Götz ◽  
Gerd Döring
Keyword(s):  
Staphylococcus Aureus ◽  
Staphylococcus Epidermidis ◽  
Biofilm Formation ◽  
Extracellular Polysaccharide ◽  
Growth Conditions ◽  
Anaerobic Conditions ◽  
Anaerobic Environment ◽  
Specific Mrna

ABSTRACT Products of the intercellular adhesion (ica) operon in Staphylococcus aureus and Staphylococcus epidermidis synthesize a linear β-1,6-linked glucosaminylglycan. This extracellular polysaccharide mediates bacterial cell-cell adhesion and is required for biofilm formation, which is thought to increase the virulence of both pathogens in association with prosthetic biomedical implants. The environmental signal(s) that triggers ica gene product and polysaccharide expression is unknown. Here we demonstrate that anaerobic in vitro growth conditions lead to increased polysaccharide expression in both S. aureus and S. epidermidis, although the regulation is less stringent inS. epidermidis. Anaerobiosis also dramatically stimulates ica-specific mRNA expression inica- and polysaccharide-positive strains of both S. aureus and S. epidermidis.These data suggest a mechanism whereby ica gene expression and polysaccharide production may act as a virulence factor in an anaerobic environment in vivo.

scholarly journals The effect of pH and ionic strength of dissolution media on in-vitro release of two model drugs of different solubilities from HPMC matrices

2013 ◽  
Vol 111 ◽  
pp. 384-391 ◽  
Author(s):  
Kofi Asare-Addo ◽  
Barbara R. Conway ◽  
Hassan Larhrib ◽  
Marina Levina ◽  
Ali R. Rajabi-Siahboomi ◽  
...  
Keyword(s):  
Ionic Strength ◽  
In Vitro Release ◽  
Effect Of Ph ◽  
Vitro Release

Reductive Activation of Carbon Tetrachloride by Human Haemoglobin

1993 ◽  
Vol 21 (1) ◽  
pp. 57-64
Author(s):  
Roberta Ferrara ◽  
Michela Rezzadore ◽  
Stefano Cazzaro ◽  
Roberto Tolando ◽  
Maurizio Manno
Keyword(s):  
Carbon Monoxide ◽  
Carbon Tetrachloride ◽  
Anaerobic Incubation ◽  
Reductive Activation ◽  
Anaerobic Conditions ◽  
Prosthetic Group ◽  
Experimental Conditions ◽  
Human Haemoglobin ◽  
Reductive Metabolism

The reductive metabolism of carbon tetrachloride (CC14) by human haemoglobin (Hb) was observed in vitro by absolute absorption spectra recorded under anaerobic conditions. The following results were obtained: 1) a decrease of the 430nm peak typical of free reduced Hb (Hb2+); 2) the formation of a shoulder of absorbance, attributable to the production of a complex between Hb2+ and a metabolite of CC14 carbon monoxide (Hb-CO); and 3) the oxidation of some Hb2+ to methaemoglobin (Hb3+). The concentration of these three forms — Hb2+, Hb-CO and Hb3+ — during anaerobic incubation of Hb with CC14 was calculated algebraically from the absolute spectra. CO production was then calculated from the concentration of Hb-CO, using a suitable calibration curve. Interestingly, under identical experimental conditions, a substrate-dependent loss of Hb-derived haem, but not of Hb itself nor of haem-derived porphyrin fluorescence, was measured. Preliminary HPLC studies to clarify the discrepancy and, in particular, the role and fate of the haem group, showed two substrate-dependent modified haem products. The results indicate that human Hb is able to catalyse the reductive activation of CCl4, and suggest that, during the process, its prosthetic group haem may be modified by CC14 metabolites to products which maintain a tetrapyrrolic structure but are unable to react with pyridine.

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