miR-106B-25 Cluster expression: a comparative human and canine osteosarcoma study

BackgroundOsteosarcoma (OS) is the most common primary malignant bone tumour in dogs and human beings, characterised by similar genetic and clinical features. With the aim to define similarities and differences in the biological aspects involved in OS progression, a comparative study was performed to create a model to improve patient outcome.MethodsFirst, the expression of microRNAs (miRNAs) belonging to the cluster miR-106b-25 (miR-106b, miR-25 and miR-93-5p) in human and canine OS tissue was compared.ResultsmiR-25 and miR-106b presented a variable expression not significantly different from the corresponding normal bone, while miR-93-5p expression was increased in all OS specimens, with higher levels in the canine subset compared with human. Accordingly, its target p21 presented a weaker and less homogeneous immunostaining distribution in the canine group. Given the high expression of miR-93-5p in all OS specimens, the functional response of human 143B and canine DAN OS cells to miRNA inhibition was evaluated. Although p21 expression increased after miR-93-5p inhibition both at mRNA and protein level, a more significant cell response in terms of proliferation and apoptosis was seen in canine OS cells.ConclusionsIn conclusion, canine OS tissue and cell line presented higher expression levels of miR-93-5p than human OS. In addition, the introduction of miR-93-5p inhibitor caused a cell response in 143B and DAN that differed for the more intense functional impact in the canine OS cell line.

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Aberrant Type C Virus Production in a Cell Line Derived from Balb/3T3

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100094693 ◽

1972 ◽

Vol 30 ◽

pp. 286-287

Author(s):

N. Savage ◽

A. Hackett

Keyword(s):

Electron Microscopy ◽

Cell Line ◽

Leukemia Virus ◽

Morphological Characteristics ◽

Virus Production ◽

Oncogenic Viruses ◽

Endogenous Virus ◽

Virus Particles ◽

Moloney Leukemia Virus ◽

A Cell

A cell line, UC1-B, which was derived from Balb/3T3 cells, maintains the same morphological characteristics of the non-transformed parental culture, and shows no evidence of spontaneous virus production. Survey by electron microscopy shows that the cell line consists of spindle-shaped cells with no unusual features and no endogenous virus particles.UC1-B cells respond to Moloney leukemia virus (MLV) infection by a change in morphology and growth pattern which is typical of cells transformed by sarcoma virus. Electron microscopy shows that the cells are now variable in shape (rounded, rhomboid, and spindle), and each cell type has some microvilli. Virtually all (90%) of the cells show virus particles developing at the cell surface and within the cytoplasm. Maturing viruses, typical of the oncogenic viruses, are found along with atypical tubular forms in the same cell.

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Establishment of a cell line (HCC-M) from a human hepatocellular carcinoma

International Journal of Cancer ◽

10.1002/ijc.2910320202 ◽

1983 ◽

Vol 32 (2) ◽

pp. 141-146 ◽

Cited By ~ 35

Author(s):

Tetsu Watanabe ◽

Toshio Morizane ◽

Kanji Tsuchimoto ◽

Yasutaka Inagaki ◽

Yoshio Munakata ◽

...

Keyword(s):

Hepatocellular Carcinoma ◽

Cell Line ◽

Human Hepatocellular Carcinoma ◽

A Cell

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2,4-Dichlorophenol and MCPA in a V79 Test

Alternatives to Laboratory Animals ◽

10.1177/026119298801500314 ◽

1988 ◽

Vol 15 (3) ◽

pp. 245-250

Author(s):

Geirid Fiskesjö

Keyword(s):

Chinese Hamster ◽

Chinese Hamster Cell ◽

Test System ◽

Similar Degree ◽

Human Beings ◽

Industrial Chemicals ◽

Allium Test ◽

Chinese Hamster Cell Line ◽

A Cell ◽

Mutagenic Effects

Two industrial chemicals, 2,4-dichlorophenol and 4-chloro-2-methylphenoxyacetic acid (MCPA), which have no toxic effects on the Chinese hamster cell line V79 alone, were tested for toxicity and mutagenicity in a cell-mediated test, where mixed-function oxidase (MFO) enzymes are active in the metabolism of xenobiotics. For 2,4-dichlorophenol, a dose-dependent toxicity as well as a slight mutagenicity could be shown when oxygenation enzymes were present. A similar degree of toxicity in a plant test system (the Allium test) indicates a similar risk of damage from exposure to dichlorophenol treatments in both these systems. MCPA did not induce any toxic or mutagenic effects at the concentrations tested. These results were not in agreement with previous results in plant material, where MCPA was clearly toxic at relatively low doses. However, since chlorophenols have been found in plants sprayed with phenoxyacetic acids, further investigations should be performed concerning potential risk to human beings.

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Human acute leukemia cell line with the t(4;11) chromosomal rearrangement exhibits B lineage and monocytic characteristics

Blood ◽

10.1182/blood.v65.1.21.21 ◽

1985 ◽

Vol 65 (1) ◽

pp. 21-31 ◽

Cited By ~ 194

Author(s):

RC Stong ◽

SJ Korsmeyer ◽

JL Parkin ◽

DC Arthur ◽

JH Kersey

Keyword(s):

Acute Leukemia ◽

Cell Line ◽

Lymphoblastic Leukemia ◽

Leukemia Cell ◽

Leukemic Cells ◽

Leukemia Cell Line ◽

Terminal Deoxynucleotidyl Transferase ◽

Immunoglobulin Gene ◽

A Cell ◽

B Lineage

Abstract A cell line, designated RS4;11, was established from the bone marrow of a patient in relapse with an acute leukemia that was characterized by the t(4;11) chromosomal abnormality. The cell line and the patient's fresh leukemic cells both had the t(4;11)(q21;q23) and an isochromosome for the long arm of No. 7. Morphologically, all cells were lymphoid in appearance. Ultrastructurally and cytochemically, approximately 30% of the cells possessed myeloid features. The cells were strongly positive for terminal deoxynucleotidyl transferase. They were HLA-DR positive and expressed surface antigens characteristic for B lineage cells, including those detected by anti-B4, BA-1, BA-2, and PI153/3. Immunoglobulin gene analysis revealed rearrangements of the heavy chain and kappa chain genes. The cells lacked the common acute lymphoblastic leukemia antigen and antigenic markers characteristic of T lineage cells. The cells reacted with the myeloid antibody 1G10 but not with other myeloid monoclonal antibodies. Treatment with 12-O-tetradecanoyl- phorbol-13-acetate induced a monocyte-like phenotype demonstrated by cytochemical, functional, immunologic, and electron microscopic studies. The expression of markers of both early lymphoid and early myeloid cells represents an unusual phenotype and suggests that RS4;11 represents a cell with dual lineage capabilities. To our knowledge, RS4;11 is the first cell line established from t(4;11)-associated acute leukemia.

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