Introgressive hybridization between Brahea dulcis and Brahea nitida (Arecaceae) in Mexico: evidence from morphological and PCR–RAPD patterns

Gene introgression usually results from spontaneous hybridization among closely related species in sympatric populations. This phenomenon has recently become a frequent topic of interest in evolutionary and conservation biology. A palm species complex occurring in Mexico formed by two native species, Brahea dulcis (Kunth) C. Martius and Brahea nitida André, and their putative hybrids, was analyzed with morphological and molecular markers in four hybrid zones and two pure sites for each parental species. Our results showed that the closeness of the hybrids to an allopatric site of either parental species (B. nitida, mesic habitat, and B. dulcis, xeric habitat) is directly related to their morphological and genetic similarity. Also, B. dulcis differed significantly from B. nitida in all the examined characters. Morphological and molecular data were highly coincident and supported the hypothesized hybridization. Hybrid phenotypes were morphologically parent-like for 46.4% of traits, intermediate for 33.2% of traits, and transgressive for 10.0% of traits. Thus, individual hybrids appear to be a mosaic of parent-like, intermediate, and transgressive phenotypes. Our results suggest that B. nitida has been genetically assimilated by B. dulcis in Xochiapala, and in the remaining hybrid zones a unidirectional pattern of gene flow towards B. nitida exists. Therefore, hybridization may threaten the conservation status of species through genetic assimilation and may confound the ability to distinguish among taxa.

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A new species of the Ranunculaceae from southwestern China based on morphological and molecular data

Phytotaxa ◽

10.11646/phytotaxa.283.1.2 ◽

2016 ◽

Vol 283 (1) ◽

pp. 43 ◽

Cited By ~ 1

Author(s):

BING LIU ◽

YUN-QING HAO ◽

YONG YANG ◽

TIAN-TIAN SUN ◽

YANG-JUN LAI ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Conservation Status ◽

Molecular Data ◽

Southwestern China ◽

Leaf Margin ◽

Molecular Systematic ◽

Field Investigations ◽

Morphological And Molecular Data ◽

A New Species

Based on field investigations, morphological and molecular systematic studies, a new species, Caltha dysosmoides (Ranunculaceae) from southwestern China is described. It differs from all other known congeneric species by the densely dentate leaf margin, the pendulous pedicels, the scarlet flowers, the compressed filaments, and the triangular connective. To better understand taxonomy of this new species, phylogenetic analyses were conducted using a combined dataset from nrITS, plastid trnL-trnF region, and atpB-rbcL spacer. The results indicate that Caltha is monophyletic and C. dysosmoides is sister to C. sinogracilis. The conservation status of the new species is categorized as CR based on IUCN criteria.

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Variability, host range, delimitation and neotypification of Amanita simulans (Amanita section Vaginatae): collections associated with Helianthemum grasslands, and epitypification of A. lividopallescens

Phytotaxa ◽

10.11646/phytotaxa.280.1.1 ◽

2016 ◽

Vol 280 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

ALFREDO VIZZINI ◽

MIRCA ZOTTI ◽

MIDO TRAVERSO ◽

ENRICO ERCOLE ◽

PIERRE-ARTHUR MOREAU ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Morphological Variability ◽

Taxonomic Status ◽

Morphological Characters ◽

Molecular Data ◽

Phylogenetic Position ◽

Extensive Discussion ◽

Closely Related Species ◽

Colour Form ◽

Morphological And Molecular Data

Several collections of Amanita species from section Vaginatae have been reported to be strictly associated with Helianthemum plants growing in grasslands, a still largely under-explored ectomycorrhizal habitat. The main aim of this study was to investigate the taxonomic status and phylogenetic position of strictly Helianthemum-associated Amanita sect. Vaginatae taxa, informally named Amanita “helianthemicola”. Collections from Italy, England and France were included in this study. The morphologically closely related species complex A. lividopallescens was also examined. Analyses were carried out based on both morphological and molecular data (phylogenetic analysis of the nrITS sequences). All investigated Amanita collections, which are strictly associated with Helianthemum nummularium, turned out to be conspecific with A. simulans. Amanita simulans was recently described from Sardinia (Italy) from Populus nigra habitats based on morphological characters only. As the holotype of A. simulans was lost, a neotype is designated here based on a voucher from the original collecting area. Amanita simulans is re-described, and an extensive discussion on the morphological variability, host species range, distribution and related taxa is provided. Amanita lividopallescens was confirmed as a good species, and re-delimited based on our phylogenetic analysis; moreover, it was epitypified with a recent and well-documented collection from Corse (France). Amanita stenospora is a synonym of A. lividopallescens, being a colour form of the latter without taxonomic value.

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First Guatemalan record of natural hybridisation between Neotropical species of the Lady’s Slipper orchid (Orchidaceae, Cypripedioideae)

PeerJ ◽

10.7717/peerj.4162 ◽

2017 ◽

Vol 5 ◽

pp. e4162 ◽

Cited By ~ 5

Author(s):

Dariusz L. Szlachetko ◽

Marta Kolanowska ◽

Fred Muller ◽

Jay Vannini ◽

Joanna Rojek ◽

...

Keyword(s):

Ecological Niche Modeling ◽

Parental Species ◽

Signal Sequence ◽

Conservation Status ◽

Hybrid Zones ◽

Maternal Line ◽

Modeling Analysis ◽

Sierra Madre ◽

Natural Hybridisation ◽

Slipper Orchid

The first natural hybrid in the sectionIrapeanaof the orchid genusCypripediumis described and illustrated based on Guatemalan material. A molecular evaluation of the discovery is provided. Specimens with intermediate flowers betweenC. irapeanumandC. dickinsonianumwithin ITS andXdhsequences have the signal sequence of both these species. The analysis of plastid sequences indicated that the maternal line isC. irapeanum. Information about the ecology, embryology and conservation status of the novelty is given, together with a distribution map of its parental species,C. irapeanumandC. dickinsonianum. A discussion of the hybridization betweenCypripediumspecies is presented. The potential hybrid zones between the representatives ofCypripediumsectionIrapeanawhich were estimated based on the results of ecological niche modeling analysis are located in the Maya Highlands (C. dickinsonianumandC. irapeanum) and the eastern part of Southern Sierra Madre (C. molleandC. irapeanum). Moreover, all threeCypripediumspecies could inhabit Cordillera Neovolcánica according to the obtained models; however, it should be noticed that this region is well-distanced from the edges of the known geographical range ofC. molle.

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Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the “Crangonyx floridanus complex”

Zootaxa ◽

10.11646/zootaxa.4691.4.1 ◽

2019 ◽

Vol 4691 (4) ◽

pp. 301-332 ◽

Cited By ~ 1

Author(s):

ANDREW G. CANNIZZARO ◽

THOMAS R. SAWICKI

Keyword(s):

New Species ◽

Related Species ◽

Species Delimitation ◽

Species Complex ◽

Morphological Characteristics ◽

Molecular Data ◽

Closely Related Species ◽

Leon County ◽

Two New Species ◽

Morphological And Molecular Data

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses.

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Cymbidium motuoense (Orchidaceae; Epidendroideae), a new species from China: evidence from morphological and molecular data

Phytotaxa ◽

10.11646/phytotaxa.509.1.5 ◽

2021 ◽

Vol 509 (1) ◽

Author(s):

WENQI HU ◽

QINGHAI ZHANG ◽

GUIZHEN CHEN ◽

MENGJIA ZHU ◽

XIA YU ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Plastid Dna ◽

Molecular Data ◽

Natural Hybrid ◽

Closely Related Species ◽

Orchid Species ◽

Molecular Analyses ◽

Morphological And Molecular Data ◽

A New Species

This study describes a new orchid species, Cymbidium motuoense, from Xizang, China based on morphological and molecular analyses. Several unique morphological features distinguish this new orchid from all other species of Cymbidium. Based on its morphology, this orchid is similar to C. tracyanum but differs in its yellow-green flower with dark purple-red spots on the veins, oblong-elliptic sepals, side lobes of the lip with reddish brown hairs along the veins and disc with three purple-red lamellae. Phylogenetic analyses based on plastid DNA (matK and rbcL) supported C. motuoense as a new species. However, the low support in nuclear ribosomal ITS tree provide the possibility that the new orchid may be a natural hybrid of C. tracyanum and C. iridioides or another closely related species.

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The “Cobra-preta” of São Tomé Island, Gulf of Guinea, is a new species of Naja Laurenti, 1768 (Squamata: Elapidae)

Zootaxa ◽

10.11646/zootaxa.4324.1.7 ◽

2017 ◽

Vol 4324 (1) ◽

pp. 121 ◽

Cited By ~ 7

Author(s):

LUIS M. P. CERÍACO ◽

MARIANA P. MARQUES ◽

ANDREAS SCHMITZ ◽

AARON M. BAUER

Keyword(s):

New Species ◽

Conservation Status ◽

Molecular Data ◽

Oceanic Islands ◽

Gulf Of Guinea ◽

Biogeographic History ◽

History Of ◽

Morphological And Molecular Data ◽

Taxonomic Groups ◽

A New Species

The Cobra-Preta (black snake in Portuguese) of Sao Tomé Island in the Gulf of Guinea has historically been referred to as Naja (Boulengerina) melanoleuca (Squamata: Elapidae). Its presence on the island has been traditionally explained as an introduction from the mainland by Portuguese settlers, supposedly to control the rat population. This explanation has been widely accepted by local authorities and even international conservation agencies. The taxonomic identity of this snake has remained undisputed by all taxonomists who have published about it, with the exception of L. Capocaccia in 1961. Arguments supporting the human introduction hypothesis are weak and are contradicted by historical, morphological and molecular data. Further, the biogeographic history of the Gulf of Guinea oceanic islands and recent insights on the taxonomic identity and evolutionary history of other taxonomic groups occurring there suggest that the Cobra-Preta, in fact, represents a distinct lineage of the melanoleuca group, endemic to São Tomé. We here describe the Cobra Preta as a new species. The new species differs from N. (B.) melanoleuca, its sister species, by a distinct coloration ventral pattern and the type of contact of the sublingual scales. Data on the toxicology, distribution, ecology, folklore and conservation status of the new species are presented.

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Croton calcareus: a new species of dragon’s blood (Euphorbiaceae) from dry forest in the state of Chiapas, Mexico

European Journal of Taxonomy ◽

10.5852/ejt.2020.657 ◽

2020 ◽

Author(s):

Leticia Mateo-Ramírez ◽

Ricarda Riina

Keyword(s):

New Species ◽

Conservation Status ◽

Species Conservation ◽

Molecular Data ◽

The State ◽

Dry Forest ◽

Small Tree ◽

Dragon’S Blood ◽

Morphological And Molecular Data ◽

A New Species

We describe Croton calcareus Riina & Mateo-Ram. sp. nov., a new species in Croton section Cyclostigma (dragon’s blood trees) from the state of Chiapas (Mexico). This species is a small tree growing in dry forest on calcareous substrates. Both morphological and molecular data support C. calcareus sp. nov. as a new species closely related to C. redolens, another dry forest taxon from northern Venezuela. We provide illustrations, a distribution map and suggestions for species conservation status. The new species along with Croton draco are the only known representatives of C. section Cyclostigma occurring in Mexico.

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Morphological ontogeny and molecular analyses of geographic strains of two closely related Neoseiulus species (Acari: Phytoseiidae)

Acarologia ◽

10.24349/acarologia/20214440 ◽

2021 ◽

Vol 61 (2) ◽

pp. 432-452

Author(s):

Mohamed W. Negm ◽

Tomoko Matsuda ◽

Takumi Kayukawa ◽

Chyi-Chen Ho ◽

Yu-Tzu Hsu ◽

...

Keyword(s):

Ribosomal Dna ◽

Related Species ◽

Molecular Data ◽

Predatory Mites ◽

Nuclear Ribosomal Dna ◽

Immature Stages ◽

Closely Related Species ◽

Molecular Analyses ◽

Neoseiulus Longispinosus ◽

Morphological And Molecular Data

The predatory mites, Neoseiulus longispinosus (Evans) and N. womersleyi (Schicha) (Phytoseiidae) are two morphologically close species. Although they can be mainly separated by the length of the dorsal seta S5, misidentifications may occur. The present study investigated various strains of the two closely related species collected from Japan, Taiwan and/or Thailand using a combination of morphological and molecular data. We described and illustrated the adults and immature stages. We also sequenced the 28S region of nuclear ribosomal DNA to determine whether these strains represent different species. The two species could be clearly separated based on the length of seta S5 and the ratio of setae Z5/S5. In a 28S tree, the examined strains also formed separate clades with 100% bootstrap values. We conclude that a combination of different methods is often necessary to precisely distinguish these closely related taxa.

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Aegla buenoi n. sp. (Decapoda: Anomura): first record of aeglid crab from Cinzas River basin, Brazil

Zootaxa ◽

10.11646/zootaxa.5005.3.4 ◽

2021 ◽

Vol 5005 (3) ◽

pp. 291-303

Author(s):

INGRID COSTA MARÇAL ◽

FERNANDA POLLI PÁEZ ◽

LENICE SOUZA-SHIBATTA ◽

SILVIA HELENA SOFIA ◽

GUSTAVO MONTEIRO TEIXEIRA

Keyword(s):

New Species ◽

River Basin ◽

Related Species ◽

First Record ◽

Molecular Data ◽

New Taxon ◽

Diagnostic Features ◽

Closely Related Species ◽

Morphological And Molecular Data ◽

A New Species

A new species of freshwater anomuran crab, Aegla buenoi n. sp., is described. The new taxon was collected from two streams within the Cinzas River basin, Paraná state, Brazil. We used morphological and molecular data (COI mtDNA) to distinguish the new species from its congeners. Aegla buenoi n. sp. is differentiated by morphological diagnostic features of the cephalothorax, chelipeds, second abdominal epimeron, and uropods. Molecular results confirm the separation of A. buenoi n. sp. from closely related species (A. castro Schmitt, 1942, A. lata Bond-Buckup & Buckup, 1994, and A. jacutinga Marçal & Teixeira, 2020). Hence, our study increases the known diversity of aeglids and reports the first species of Aegla from the Cinzas River basin.

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Morphological and molecular data show Synostemon trachyspermus (Phyllanthaceae, Phyllantheae) to be a heterogeneous species assemblage

Australian Systematic Botany ◽

10.1071/sb16008 ◽

2016 ◽

Vol 29 (3) ◽

pp. 218 ◽

Cited By ~ 3

Author(s):

Ian R. H. Telford ◽

Kanchana Pruesapan ◽

Peter C. van Welzen ◽

Jeremy J. Bruhl

Keyword(s):

Western Australia ◽

Sequence Data ◽

Conservation Status ◽

Molecular Data ◽

New Combination ◽

Species Assemblage ◽

Dna Sequence Data ◽

The North ◽

Morphological Studies ◽

Morphological And Molecular Data

Synostemon trachyspermus (F.Muell.) I.Telford & Pruesapan (Phyllanthaceae, Phyllantheae) is shown, by morphological studies and phylogenetic analysis using nrITS DNA sequence data, to be a heterogeneous species assemblage of four species. Phyllanthus rhytidospermus F.Muell. ex Müll.Arg., with a new combination provided as Synostemon rhytidospermus (F.Muell. ex Müll.Arg.) I.Telford & Pruesapan, and Sauropus hubbardii Airy Shaw, with a new combination as Synostemon hubbardii (Airy Shaw) I.Telford & Pruesapan, are re-instated as species. Phyllanthus arnhemicus S.Moore is lectotypified and placed in synonomy under Synostemon lissocarpus (S.Moore) I.Telford & Pruesapan, which is the new combination provided for Phyllanthus lissocarpus S.Moore (syn. Sauropus lissocarpus (S.Moore) Airy Shaw). Synostemon umbrosus I.Telford & J.J.Bruhl, a rare endemic from the Kimberley, Western Australia, is named as new. The newly described S. hamersleyensis I.Telford & Naaykens, endemic to the Pilbara, Western Australia, and the north-eastern Queensland endemic Sauropus aphyllus J.T.Hunter & J.J.Bruhl are shown to be closely related; the new combination Synostemon aphyllus (J.T.Hunter & J.J.Bruhl) I.Telford & Pruesapan is provided for the latter. Sauropus sp. A of Flora of the Kimberley Region, previously included within S. trachyspermus sens.lat., shows a more distant relationship and is named as Synostemon judithae I.Telford & J.J.Bruhl. Notes on distribution, habitat, phenology, conservation status, photomicrographs of seeds and a key to identification of the species are provided.

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