The ultrastructure of zoospores of Phytophthora megasperma var. sojae

1968 ◽  
Vol 46 (1) ◽  
pp. 37-41 ◽  
Author(s):  
H. H. Ho ◽  
K. Zachariah ◽  
C. J. Hickman

The zoospore of Phytophthora megasperma var. sojae is bounded by a unit membrane. Ribosomes, mitochondria, and vesicles with amorphous or crystalline contents occur throughout the zoospore with the exception of the groove region which is characterized by the presence of microtubules, transversely striated elements of unknown nature, and a large vacuole. The flagella, with typical 9 + 2 fibril arrangement, are inserted on a small ridge which lies across the groove. Conspicuous rough endoplasmic reticulum is present in the form of bands around the nucleus and in the cytoplasm. Interspersed amongst the vesicles, just below the surface of the zoospore, are some bodies resembling microbodies or spherosomes.


Author(s):  
T. N. Tahmisian ◽  
E. J. Ainsworth

Studies are in progress to characterize a transmissible leukemia and the causal agent which is presumably viral. The neoplasm, originally isolated from an aged irradiated B6CF1, mouse, has been transmitted in irradiated (200 R; 250 kVp X rays) weanling mice by intraperitoneal injection of millipore filtered (0.45μ) supernatant from spleen cell suspensions. At 22 days after filtrate injection in syngeneic mice, spleen, lymph nodes, and thymus were removed and prepared for electron microscopy to determine the presence of virus and morphology of viral development.The ultrastructure of cells from the above organs showed viral infection by a murine type leukemia virus with many “C” type buds 90 to 110nm formed in the cisternae of the rough endoplasmic reticulum (Fig. 1). The limiting membrane of the “C” type virus, apparently isolating it from the cytoplasm, is the unit membrane from the rough endoplasmic reticulum. The viral membrane is not invariably intact.



Author(s):  
R. A. Turner ◽  
A. E. Rodin ◽  
D. K. Roberts

There have been many reports which establish a relationship between the pineal and sexual structures, including gonadal hypertrophy after pinealectomy, and gonadal atrophy after injection of pineal homogenates or of melatonin. In order to further delineate this relationship the pineals from 5 groups of female rats were studied by electron microscopy:ControlsPregnant ratsAfter 4 weekly injections of 0.1 mg. estradiol benzoate.After 8 daily injections of 150 mcgm. melatonin (pineal hormone).After 8 daily injections of 3 mg. serotonin (melatonin precursor).No ultrastructural differences were evident between the control, and the pregnancy and melatonin groups. However, the estradiol injected animals exhibited a marked increase in the amount and size of rough endoplasmic reticulum within the pineal cells.



Author(s):  
V.J. Montpetit ◽  
S. Dancea ◽  
L. Tryphonas ◽  
D.F. Clapin

Very large doses of pyridoxine (vitamin B6) are neurotoxic in humans, selectively affecting the peripheral sensory nerves. We have undertaken a study of the morphological and biochemical aspects of pyridoxine neurotoxicity in an animal model system. Early morphological changes in dorsal root ganglia (DRG) associated with pyridoxine megadoses include proliferation of neurofilaments, ribosomes, rough endoplasmic reticulum, and Golgi complexes. We present in this report evidence of the formation of unique aggregates of microtubules and membranes in the proximal processes of DRG which are induced by high levels of pyridoxine.





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