Anatomy and ultrastructure of a Rhododendron root–fungus association

1980 ◽  
Vol 58 (23) ◽  
pp. 2421-2433 ◽  
Author(s):  
T. A. Peterson ◽  
W. C. Mueller ◽  
L. Englander
Keyword(s):  
Fungal Infection ◽  
Secondary Growth ◽  
Cortical Layer ◽  
Electron Microscopic ◽  
Cortical Cells ◽  
Host Cytoplasm ◽  
Hair Roots ◽  
Short Period ◽  
Infected Cells ◽  
Hyphal Coils

Light and electron microscopic investigations of the roots of Rhododendron and other ericaceous plants growing in the vicinity of Clavaria fruiting structures showed a fungal infection consistently associated with the epidermal and cortical cells of the "hair roots." Uninfected hair roots consisted of an epidermis and a one cell thick cortical layer surrounding the stele. Secondary growth in the stele and formation of a cork layer by division of the pericycle caused the cortex and epidermis to slough as the root matured. The structure of the infected hair roots was similar except for the presence of fungus in epidermal and cortical cells. As judged by the appearance of septa, at least two fungi were involved, one with dolipore septa that formed hyphal coils in the infected cells, and one with septa associated with Woronin bodies that occurred as single hyphal strands. Hyphae were found penetrating the cells from the exterior of the root and also passing from cell to cell. No correlation between fungal infection and the phenolic content of the cells could be made. Dissolution of both the fungal and host cytoplasm appeared to occur as the cells were sloughed. It appears that the fungus–root relationship is complex and is limited in duration to a short period of time during the development of the hair roots.

Structure and host–actinomycete interactions in developing root nodules of Comptonia peregrina

1978 ◽  
Vol 56 (5) ◽  
pp. 502-531 ◽  
Author(s):  
William Newcomb ◽  
R. L. Peterson ◽  
Dale Callaham ◽  
John G. Torrey
Keyword(s):  
Host Cell ◽  
Root Nodules ◽  
Host Cells ◽  
Electron Microscopic ◽  
Cortical Cells ◽  
Host Cytoplasm ◽  
Transmission Electron ◽  
Microscopic Studies ◽  
Host Plasma Membrane ◽  
Soil Actinomycete

Correlated fluorescence, bright-field, transmission electron, and scanning electron microscopic studies were made on developing root nodules of Comptonia peregrina (L.) Coult. (Myricaceae) produced by a soil actinomycete which invades the root and establishes a symbiosis leading to fixation of atmospheric dinitrogen. After entering the host via a root hair infection, the hyphae of the endophyte perforate root cortical cells by local degradation of host cell walls and penetration of the host cytoplasm. The intracellular hyphae are always surrounded by host plasma membrane and a thick polysaccharide material termed the capsule. (For convenience, term intracellular refers to the endophyte being inside a Comptonia cell as distinguished from being intercellular, i.e.. between host cells, even though the former is actually extracellular as the endophyte is separated from the host cytoplasm by the host plasmalemma.) Numerous profiles of vesiculate rough endoplasmic reticulum (RER) occur near the growing hyphae. Although the capsule shows a positive Thiery reaction indicating its polysaccharide nature, the fibrillar contents of the RER do not, leaving uncertain whether the capsule results from polymers derived from the RER. Amyloplasts of the cortical cells lose their starch deposits during hyphal proliferation. The hyphae branch extensively in specific layers of the cortex, penetrating much of the host cytoplasm. At this stage, hyphal ends become swollen and form septate club-shaped vesicles within the periphery of the host cells. Lipid-like inclusions and Thiery-positive particles, possibly glycogen, are observed in the hyphae at this time. Associated with hyphal development is an increase in average host cell volume, although nuclear volume appears to remain constant. Concomitant with vesicle maturation, the mitochondrial population increases sharply, suggesting a possible relationship to vesicle function. The intimate interactions between host and endophyte during development of the symbiotic relationship are emphasized throughout.

A correlated light and electron microscopic study of symbiotic growth and differentiation in Pisum sativum root nodules

1976 ◽  
Vol 54 (18) ◽  
pp. 2163-2186 ◽  
Author(s):  
William Newcomb
Keyword(s):  
Electron Microscopy ◽  
Pisum Sativum ◽  
Rhizobium Leguminosarum ◽  
Root Nodules ◽  
Light And Electron Microscopy ◽  
Infection Thread ◽  
Electron Microscopic ◽  
Cortical Cells ◽  
Garden Pea ◽  
Host Cytoplasm

Plants of the garden pea Pisum sativum cv. Little Marvel were grown in aeroponic culture to facilitate observations and microscopy and were inoculated with Rhizobium leguminosarum, and nodules were sampled at five weekly intervals for light and electron microscopy. The invasion of the cortical cells by the infection thread, the structure of the infection thread, and the release of bacteria from it into the host cytoplasm and the subsequent symbiotic growth and differentiation of the two organisms are described in detail. The fine structure of the nodule is correlated with light microscopic observations and morphogenesis. A restriction in the use of the term 'vesicle' is proposed because of the current multiple and confusing usage of the term. The loss of the nodule meristem and its morphogenetic significance are discussed.

A comparative cytological analysis of fungal endophytes in the sporophyte rhizomes and vascularized gametophytes of Tmesipteris and Psilotum

2005 ◽  
Vol 83 (11) ◽  
pp. 1443-1456 ◽  
Author(s):  
Jeffrey G. Duckett ◽  
Roberto Ligrone
Keyword(s):  
Fungal Endophytes ◽  
Vascular Anatomy ◽  
Arbuscular Mycorrhizas ◽  
Wall Material ◽  
Electron Microscopic ◽  
Cortical Cells ◽  
Vascular Elements ◽  
Vessel Elements ◽  
Perforation Plates ◽  
Hyphal Coils

This article describes the results of a light and electron microscopic study of the fungal endophytes and vascular anatomy in the rhizomes and gametophytes of Tmesipteris and Psilotum. The parenchymatous cortical cells of the rhizomes and subterranean gametophytes of Tmesipteris and Psilotum contain intracellular aseptate glomeromycotean fungi resembling the “Paris-type” of arbuscular mycorrhizas found in seed plants. The fungi differentiate into multinucleate vesicles and hyphal coils, both containing bacteria-like structures and accumulating lipid masses and crystals as they age. After several cycles of infection in the same cell, degenerate hyphae form amorphous masses encased by host wall material. Nearly identical host–fungus cytology between the autotrophic sporophytes and the heterotrophic gametophytes suggests that these psilophyte associations are exploitative of the fungus in both generations. Following the description of tracheids nearly 60 years ago in the gametophytes of Psilotum, vascular elements are described for the first time in the haploid generation of Tmesipteris. Close similarities between the water- and food-conducting elements in both generations, viz. vessel elements with scalariform perforation plates and sieve cells with refractive spherules and lacking callose at all stages in their develoment, add support to the homologous theory of the alternations of generations. Mitochondrial aggregations, cross-linked by small electron-opaque rods, are common in the stelar cells of both generations and appear to be a unique feature of the psilophyte clade.

Dynamics of arbuscule development and degeneration in onion, bean, and tomato with reference to vesicular–arbuscular mycorrhizae in grasses

1989 ◽  
Vol 67 (8) ◽  
pp. 2505-2513 ◽  
Author(s):  
Tom Alexander ◽  
Ronald Toth ◽  
Rose Meier ◽  
Hans Christian Weber
Keyword(s):  
Host Cell ◽  
Cell Volume ◽  
Arbuscular Mycorrhizae ◽  
Arbuscular Mycorrhizal Fungus ◽  
Mycorrhizal Fungus ◽  
Arbuscular Mycorrhizal ◽  
Electron Microscopic ◽  
Cortical Cells ◽  
Host Cytoplasm ◽  
Vesicular Arbuscular

A quantitative light and electron microscopic study of developing and degenerating arbuscules of the vesicular–arbuscular mycorrhizal fungus Glomus fasciculatum in onion, bean, and tomato was carried out to estimate three parameters during the colonization cycle and to compare these parameters with those in maize, oats, and wheat. The parameters are (i) Vv(a,c) the fraction of the host cell volume (c) occupied by the arbuscule (a); (ii) VV(cy,c) the fraction of the host cell volume occupied by host cytoplasm (cy); and (iii) SV(p,c) the ratio of the surface area of the host protoplast (p) to the volume of the whole host cell. Uninfected cortical cells contained 3.4% cytoplasm in onion, 3.1% in bean, and 3.5% in tomato. In cells with mature arbuscules, cytoplasm increased to 9.9% in onion, 14.2% in bean, and 13.6% in tomato. Cells with mature arbuscules contained 11.4% fungus in onion, 20.3% in bean, and 20.5% in tomato. The initial SV(p,c) in onion was 0.10 μm2/μm3 and in bean and tomato 0.11 μm2/μm3. This increased to 0.37 μm2/μm3 in onion, 0.82 μm2/μm3 in bean, and 0.54 μm2/μm3 in tomato by the time arbuscules were mature. Development of the arbuscule was estimated to take 2.5 days and occupied 33% of the total cycle time. The variation seen across host species can be used as an indicator of fungal and (or) host control for each parameter. Arbuscular parameters of onion were compared with those obtained by other authors.

Further Observations on the Virus of Epizootic Diarrhea of Infant Mice. An Electron Microscopic Study

1967 ◽  
Vol 25 ◽  
pp. 110-111
Author(s):  
W. G. Banfield ◽  
G. Kasnic ◽  
J. H. Blackwell
Keyword(s):  
Electron Microscope ◽  
Infected Cell ◽  
Microscopic Study ◽  
Intestinal Epithelium ◽  
Ultrastructural Study ◽  
Osmium Tetroxide ◽  
Lead Citrate ◽  
Electron Microscopic ◽  
Virus Particles ◽  
Infected Cells

An ultrastructural study of the intestinal epithelium of mice infected with the agent of epizootic diarrhea of infant mice (EDIM virus) was first performed by Adams and Kraft. We have extended their observations and have found developmental forms of the virus and associated structures not reported by them.Three-day-old NLM strain mice were infected with EDIM virus and killed 48 to 168 hours later. Specimens of bowel were fixed in glutaraldehyde, post fixed in osmium tetroxide and embedded in epon. Sections were stained with uranyl magnesium acetate followed by lead citrate and examined in an updated RCA EMU-3F electron microscope.The cells containing virus particles (infected) are at the tips of the villi and occur throughout the intestine from duodenum through colon. All developmental forms of the virus are present from 48 to 168 hours after infection. Figure 1 is of cells without virus particles and figure 2 is of an infected cell. The nucleus and cytoplasm of the infected cells appear clearer than the cells without virus particles.

scholarly journals Zika virus replication in glioblastoma cells: electron microscopic tomography shows 3D arrangement of endoplasmic reticulum, replication organelles, and viral ribonucleoproteins

2021 ◽  
Author(s):  
Johannes Wieland ◽  
Stefan Frey ◽  
Ulrich Rupp ◽  
Sandra Essbauer ◽  
Rüdiger Groß ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Line ◽  
Zika Virus ◽  
Structural Changes ◽  
Electron Tomography ◽  
Glioblastoma Cells ◽  
Electron Microscopic ◽  
N Protein ◽  
Ribonucleoprotein Complexes ◽  
Infected Cells

AbstractStructural changes of two patient-derived glioblastoma cell lines after Zika virus infection were investigated using scanning transmission electron tomography on high-pressure-frozen, freeze-substituted samples. In Zika-virus-infected cells, Golgi structures were barely visible under an electron microscope, and viral factories appeared. The cytosol outside of the viral factories resembled the cytosol of uninfected cells. The viral factories contained largely deranged endoplasmic reticulum (ER), filled with many so-called replication organelles consisting of a luminal vesicle surrounded by the ER membrane. Viral capsids were observed in the vicinity of the replication organelles (cell line #12537 GB) or in ER cisternae at large distance from the replication organelles (cell line #15747 GB). Near the replication organelles, we observed many about 100-nm-long filaments that may represent viral ribonucleoprotein complexes (RNPs), which consist of the RNA genome and N protein oligomers. In addition, we compared Zika-virus-infected cells with cells infected with a phlebovirus (sandfly fever Turkey virus). Zika virions are formed in the ER, whereas phlebovirus virions are assembled in the Golgi apparatus. Our findings will help to understand the replication cycle in the virus factories and the building of the replication organelles in glioblastoma cells.

scholarly journals ELECTRON MICROSCOPIC STUDY ON ENLARGED CELLS OF RED SEA BREAM, Pagrus major INFECTED BY THE RED SEA BREAM IRIDOVIRUS (RSIV, GENUS Megalocytivirus, FAMILY Iridoviridae)

2009 ◽  
Vol 4 (1) ◽  
pp. 53
Author(s):  
Ketut Mahardika
Keyword(s):  
Electron Microscope ◽  
Inclusion Body ◽  
Red Sea ◽  
Virus Assembly ◽  
Infected Fish ◽  
Sea Bream ◽  
Red Sea Bream ◽  
Electron Microscopic ◽  
Target Organs ◽  
Infected Cells

Most histopathologycal studies of the red sea bream iridovirus (RSIV) disease in red sea bream have been performed by studying enlarged cells as well as necrotized cells in the spleen and other organs. These enlarged cells have been named as inclusion body bearing cells (IBCs). However, few information is available about detail of ultrastructural features of IBCs produced in the target organs of RSIV-infected fish. In the present study, details of ultrastructural features of IBCs that were produced in the spleen tissue of naturally RSIV-infected red sea bream were investigated under electron microscope. Under electron microscope, RSIV-infected red sea bream had the presence of two types of IBCs: typical IBCs allowing virus assembly within viral assembly site (VAS), and atypical IBCs which degenerate organelles without virus assembly. Other infected-cells were observed as necrotized cells forming intracytoplasmic VAS with large numbers of virions, but without the formation of the distinct inclusion body. Morphogenesis steps on RSIV-infected red sea bream were observed as filamentous-filed virions, partially-filled virions and complete virions with 145-150 nm in size. These findings confirmed that RSIV-infected red sea bream were characterized by formation of typical and atypical IBCs as well as necrotized cells.

Morphological and biochemical changes in peanut nodules during photosynthate stress

1992 ◽  
Vol 38 (6) ◽  
pp. 526-533 ◽  
Author(s):  
A. B. M. Siddique ◽  
A. K. Bal
Keyword(s):  
Nitrogen Fixation ◽  
Root Nodules ◽  
Ultrastructural Level ◽  
Malate Synthase ◽  
Morphological Evidence ◽  
Lipid Bodies ◽  
Biochemical Status ◽  
Short Period ◽  
Infected Cells ◽  
Ultrastructural Damage

Nitrogen fixation in legume root nodules is believed to be supported by the supply of photosynthate of the current photoperiod. However, in peanut nodules, prolonged periods of darkness or detopping do not disrupt nitrogen fixation for at least 48 h. During this period, nodule oleosomes (lipid bodies) have been shown to decrease in number within the infected cells, and it has been suggested that lipids from oleosomes are mobilized to maintain the energy and carbon requirements of the nitrogen-fixing nodules. We present morphological evidence, at the ultrastructural level, for the utilization of oleosomes during photosynthate stress. The biochemical status of the nodule has also been assessed and correlated with ultrastructure. For comparison cowpea nodules were used that totally lacked oleosomes. In peanut nodules leghemoglobin and total protein remained unchanged along with integrated ultrastructure on nodule cells for 48 h, whereas in cowpea a decline in proteins with ultrastructural damage became apparent within a very short period of photosynthate stress. In peanut nodules empty or partially empty oleosomes were taken as evidence for their utilization during the stress period. Key words: N2 fixation, photosynthate stress, lipid bodies, catalase, malate synthase, peanut nodule, β-oxidation.

scholarly journals Characterization of the Coronavirus Mouse Hepatitis Virus Strain A59 Small Membrane Protein E

2000 ◽  
Vol 74 (5) ◽  
pp. 2333-2342 ◽  
Author(s):  
Martin J. B. Raamsman ◽  
Jacomine Krijnse Locker ◽  
Alphons de Hooge ◽  
Antoine A. F. de Vries ◽  
Gareth Griffiths ◽  
...  
Keyword(s):  
Virus Strain ◽  
Hepatitis Virus ◽  
Expression System ◽  
Mouse Hepatitis Virus ◽  
Viral Envelope ◽  
Membrane Structures ◽  
Electron Microscopic ◽  
E Protein ◽  
Infected Cells ◽  
Virus Expression

ABSTRACT The small envelope (E) protein has recently been shown to play an essential role in the assembly of coronaviruses. Expression studies revealed that for formation of the viral envelope, actually only the E protein and the membrane (M) protein are required. Since little is known about this generally low-abundance virion component, we have characterized the E protein of mouse hepatitis virus strain A59 (MHV-A59), an 83-residue polypeptide. Using an antiserum to the hydrophilic carboxy terminus of this otherwise hydrophobic protein, we found that the E protein was synthesized in infected cells with similar kinetics as the other viral structural proteins. The protein appeared to be quite stable both during infection and when expressed individually using a vaccinia virus expression system. Consistent with the lack of a predicted cleavage site, the protein was found to become integrated in membranes without involvement of a cleaved signal peptide, nor were any other modifications of the polypeptide observed. Immunofluorescence analysis of cells expressing the E protein demonstrated that the hydrophilic tail is exposed on the cytoplasmic side. Accordingly, this domain of the protein could not be detected on the outside of virions but appeared to be inside, where it was protected from proteolytic degradation. The results lead to a topological model in which the polypeptide is buried within the membrane, spanning the lipid bilayer once, possibly twice, and exposing only its carboxy-terminal domain. Finally, electron microscopic studies demonstrated that expression of the E protein in cells induced the formation of characteristic membrane structures also observed in MHV-A59-infected cells, apparently consisting of masses of tubular, smooth, convoluted membranes. As judged by their colabeling with antibodies to E and to Rab-1, a marker for the intermediate compartment and endoplasmic reticulum, the E protein accumulates in and induces curvature into these pre-Golgi membranes where coronaviruses have been shown earlier to assemble by budding.

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