Kinetics of iron transport into the leaf symplast during recovery from iron stress

1983 ◽  
Vol 61 (9) ◽  
pp. 2496-2499 ◽  
Author(s):  
Terry Forrest Young ◽  
Norman Terry

The iron concentrations of chloroplasts (isolated by nonaqueous methods) and of chloroplast lamellae (isolated by aqueous procedures) were determined and used to investigate the kinetics of iron transport across the plasmalemma into the leaf symplast during the recovery of sugar beet plants from iron stress due to iron deprivation. Iron concentrations in chloroplasts isolated by the nonaqueous procedure were greatly reduced during iron stress and recovered to over 200% of control levels within 24 h. Iron concentrations in lamellae from aqueous isolations increased significantly within 24 h of resupply and reached values in excess of control levels in 48–72 h. These results indicate that a substantial quantity of the iron that entered the leaf blade during the first 24–48 h of iron resupply crossed the plasmalemma. The effect appeared to be specific to iron because there was no evidence that manganese, zinc, and copper were transported into chloroplasts and lamellae in enhanced amounts.


1984 ◽  
Vol 62 (2) ◽  
pp. 207-210 ◽  
Author(s):  
Terry F. Young ◽  
Norman Terry

Cobalt treatment of iron-stressed sugar beet plants resulted in leaf cobalt concentrations nearly three times as high as in cobalt-treated control plants. When iron-stressed plants were treated with cobalt and iron together, cobalt was transported into leaves in preference to iron. The transport system in iron-stressed sugar beet appears to be relatively specific for iron and cobalt because iron-stressed plants supplied with manganese, zinc, and copper did not transport these elements in similarly enhanced amounts. Measurements of metal contents of chloroplasts isolated nonaqueously showed that both cobalt and iron were readily transported into chloroplasts (and therefore the leaf symplast) within 48 h. The implications of these results on the mechanism of iron transport across the root plasmalemma are discussed.



2014 ◽  
pp. 228-231 ◽  
Author(s):  
Maciej Wojtczak ◽  
Aneta Antczak-Chrobot ◽  
Edyta Chmal-Fudali ◽  
Agnieszka Papiewska

The aim of the study is to evaluate the kinetics of the synthesis of dextran and other bacterial metabolites as markers of microbiological contamination of sugar beet.



1979 ◽  
Vol 30 (6) ◽  
pp. 741 ◽  
Author(s):  
WG Jones ◽  
KF Walker

The accumulation of iron, manganese, zinc and cadmium by freshwater mussels in the River Murray, South Australia, and their response to changes in environmental iron concentrations are considered. Metal loads varied markedly between individuals from the same population. The variability is accounted for partly by systematic relationships between metal loads and body weight and age, but not sex. The distribution of metals between the major organs is discussed, but the analysis of separate organs showed no advantage for biological monitoring. Comparisons between iron concentrations in river water and in mussels showed no clear correspondence. The study suggests that V. ambiguus may not be a good short-term monitor of iron, but still may have potential as a long-term and site-comparison monitor of metals. once inherent variability is taken into account.



2004 ◽  
Vol 34 (5) ◽  
pp. 505-512 ◽  
Author(s):  
I.-E. Baciu ◽  
H.-J. Jördening


2018 ◽  
Vol 47 (20) ◽  
pp. 6954-6964
Author(s):  
James M. Harrington ◽  
Manu M. Mysore ◽  
Alvin L. Crumbliss

Catalysis of iron(iii) exchange between two chelators by a bidentate siderophore mimic is discussed in the context of iron homeostasis.



2005 ◽  
Vol 288 (5) ◽  
pp. G964-G971 ◽  
Author(s):  
James F. Collins ◽  
Christina A. Franck ◽  
Kris V. Kowdley ◽  
Fayez K. Ghishan

We sought to identify novel genes involved in intestinal iron absorption by inducing iron deficiency in rats during postnatal development from the suckling period through adulthood. We then performed comparative gene chip analyses (RAE230A and RAE230B chips; Affymetrix) with cRNA derived from duodenal mucosa. Real-time PCR was used to confirm changes in gene expression. Genes encoding the apical iron transport-related proteins [ divalent metal transporter 1 (DMT1) and duodenal cytochrome b] were strongly induced at all ages studied, whereas increases in mRNA encoding the basolateral proteins iron-regulated gene 1 and hephaestin were observed only by real-time PCR. In addition, transferrin receptor 1 and heme oxygenase 1 were induced. We also identified induction of novel genes not previously associated with intestinal iron transport. The Menkes copper ATPase (ATP7a) and metallothionein were strongly induced at all ages studied, suggesting increased copper absorption by enterocytes during iron deficiency. We also found significantly increased liver copper levels in 7- to 12-wk-old iron-deficient rats. Also upregulated at most ages examined were the sodium-dependent vitamin C transporter, tripartite motif protein 27, aquaporin 4, lipocalin-interacting membrane receptor, and the breast cancer-resistance protein (ABCG2). Some genes also showed decreased expression with iron deprivation, including several membrane transporters, metabolic enzymes, and genes involved in the oxidative stress response. We speculate that dietary iron deprivation leads to increased intestinal copper absorption via DMT1 on the brush-border membrane and the Menkes copper ATPase on the basolateral membrane. These findings may thus explain copper loading in the iron-deficient state. We also demonstrate that many other novel genes may be differentially regulated in the setting of iron deprivation.





2007 ◽  
Vol 9 (3) ◽  
pp. 317-327 ◽  
Author(s):  
LUIS M. VACCAREZZA ◽  
JOSÉ L. LOMBARDI ◽  
JORGE CHIRIFE


2012 ◽  
Vol 20 (4) ◽  
pp. 2472-2483 ◽  
Author(s):  
Arzu Y. Dursun ◽  
Ozlem Tepe ◽  
Gülşad Uslu ◽  
Gülbeyi Dursun ◽  
Yusuf Saatci


2002 ◽  
Vol 33 (19-20) ◽  
pp. 3635-3651 ◽  
Author(s):  
Kuldip Kumar ◽  
Carl J. Rosen ◽  
Satish C. Gupta


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