Isoenzyme and protein patterns of isolates of Cryphonectria cubensis differing in virulence

1984 ◽  
Vol 62 (8) ◽  
pp. 1756-1762 ◽  
Author(s):  
A. C. Alfenas ◽  
R. Jeng ◽  
M. Hubbes
Keyword(s):  
Gel Electrophoresis ◽  
Soluble Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Patterns ◽  
Eucalyptus Pellita

Isoenzyme and protein patterns of five isolates of Cryphonectria cubensis were determined by polyacrylamide gel electrophoresis. Differences in esterase, peroxidase, phosphoglucomutase, and hexokinase were detected between the Hawaiian (HW) isolate and the Brasilian (BR22) and African (AF100) isolates. These differences coincided with the virulence of the isolates tested on Eucalyptus pellita. The same tendency was also observed in their soluble protein patterns.

Optimizing soluble protein extraction and two-dimensional polyacrylamide gel electrophoresis quality for extremophile ciliates

2008 ◽  
Vol 29 (11) ◽  
pp. 2411-2412 ◽  
Author(s):  
Lorenzo Fulgentini ◽  
Roberto Marangoni ◽  
Giuliano Colombetti
Keyword(s):  
Gel Electrophoresis ◽  
Soluble Protein ◽  
Protein Extraction ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Two Dimensional

The comparison of seed protein patterns within the genusArachis by polyacrylamide gel electrophoresis

1983 ◽  
Vol 25 (4) ◽  
pp. 266-273 ◽  
Author(s):  
Eva Klozová ◽  
Jiřina Švachulová ◽  
J. Smartt ◽  
E. Hadač ◽  
Věra Turková ◽  
...  
Keyword(s):  
Gel Electrophoresis ◽  
Seed Protein ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Patterns

Colinearity of protein patterns in polyacrylamide gel electrophoresis

1972 ◽  
Vol 3 (18) ◽  
pp. 657-664 ◽  
Author(s):  
Drasko Serman ◽  
Nikola Skreb
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Polyacrylamide Gel ◽  
Protein Patterns

Relationships among species of Verticillium: protein composition of spores and mycelium

1971 ◽  
Vol 49 (8) ◽  
pp. 1293-1297 ◽  
Author(s):  
Gilles Pelletier ◽  
Robert Hall
Keyword(s):  
Gel Electrophoresis ◽  
Polyacrylamide Gel Electrophoresis ◽  
Morphological Differentiation ◽  
Protein Composition ◽  
Soluble Proteins ◽  
Polyacrylamide Gel ◽  
Close Similarity ◽  
Protein Patterns ◽  
Electrophoresis Of Proteins

Buffer-soluble proteins extracted from six morphologically different isolates of Verticillium were separated by polyacrylamide gel-electrophoresis. Protein patterns from the six isolates were different from one another whether extracts were prepared from conidia, from young colonies composed of mycelium and conidia, or from 6-day-old mycelium. However, the nature of the patterns, and therefore the degree of differences among species patterns, was influenced by the types of cells from which the extracts were prepared.Patterns of proteins from V. tricorpus, V. nigrescens, and an isolate of uncertain identity (isolate 2) which produced chlamydospores and dark mycelium were clearly different from one another whether extracts were prepared from conidia or mycelium. In contrast, conidia of V. albo-atrum, of V. dahliae, and of an isolate which did not produce pigmented structures produced very similar patterns which differed by only a few protein bands. This close similarity of patterns supports the view that V. albo-atrum and V. dahliae are genetically closely related.The protein composition of conidia differed from that of mycelium. In V. albo-atrum, spore extracts contained at least three proteins not detected in mycelium extracts. Differences between spores and mycelium were even greater in V. nigrescens and isolate 2. Analysis of V. dahliae showed differences between spores, 3-day-old mycelium, and 6-day-old mycelium.Our results support the view that gel-electrophoresis of proteins is useful as a taxonomic tool provided attention is given to the degree of morphological differentiation of the materials to be compared.

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