High-affinity phosphate absorption is independent of the root cell wall in Pisum sativum

1987 ◽  
Vol 65 (7) ◽  
pp. 1504-1508 ◽  
Author(s):  
Daniel D. Lefebvre ◽  
David T. Clarkson
Keyword(s):  
Cell Wall ◽  
Phosphate Uptake ◽  
Osmotic Shock ◽  
Protoplast Isolation ◽  
Bathing Solution ◽  
Concentration Increase ◽  
Root Cells ◽  
High Affinity ◽  
Phosphate Influx ◽  
Root Cell Wall

The high affinity for phosphate influx by pea root cells was not significantly altered by osmotic shock, plasmolysis, or the preparation of free protoplasts. The Km of normal roots was 7.74 ± 2.14 μM. The Vmax for phosphate uptake was reduced to 64% by osmotic shock and to 38 and 23% by plasmolysis and protoplast isolation, respectively. Efflux analysis indicated that there was a concentration increase for phosphate in the root free space over that of the bathing solution. This apparent binding does not appear to be involved in the uptake affinity of phosphate.

scholarly journals The Craterostigma plantagineum protein kinase CpWAK1 interacts with pectin and integrates different environmental signals in the cell wall

Planta ◽  
2021 ◽  
Vol 253 (5) ◽  
Author(s):  
Peilei Chen ◽  
Valentino Giarola ◽  
Dorothea Bartels
Keyword(s):  
Cell Wall ◽  
Stress Responses ◽  
Expression Profiles ◽  
Phylogenetic Analyses ◽  
Cell Wall Protein ◽  
Biological Processes ◽  
Environmental Signals ◽  
High Affinity ◽  
Craterostigma Plantagineum ◽  
Receptor Kinases

Abstract Main conclusion The cell wall protein CpWAK1 interacts with pectin, participates in decoding cell wall signals, and induces different downstream responses. Abstract Cell wall-associated protein kinases (WAKs) are transmembrane receptor kinases. In the desiccation-tolerant resurrection plant Craterostigma plantagineum, CpWAK1 has been shown to be involved in stress responses and cell expansion by forming a complex with the C. plantagineum glycine-rich protein1 (CpGRP1). This prompted us to extend the studies of WAK genes in C. plantagineum. The phylogenetic analyses of WAKs from C. plantagineum and from other species suggest that these genes have been duplicated after species divergence. Expression profiles indicate that CpWAKs are involved in various biological processes, including dehydration-induced responses and SA- and JA-related reactions to pathogens and wounding. CpWAK1 shows a high affinity for “egg-box” pectin structures. ELISA assays revealed that the binding of CpWAKs to pectins is modulated by CpGRP1 and it depends on the apoplastic pH. The formation of CpWAK multimers is the prerequisite for the CpWAK–pectin binding. Different pectin extracts lead to opposite trends of CpWAK–pectin binding in the presence of Ca2+ at pH 8. These observations demonstrate that CpWAKs can potentially discriminate and integrate cell wall signals generated by diverse stimuli, in concert with other elements, such as CpGRP1, pHapo, Ca2+[apo], and via the formation of CpWAK multimers.

Ascorbic Acid and the Oxidative Processes in Pea Root Cell Wall Isolates: Characterization by Fluorescence and EPR Spectroscopy

2005 ◽  
Vol 1048 (1) ◽  
pp. 500-504 ◽  
Author(s):  
SONJA VELJOVIĆ-JOVANOVIĆ ◽  
BILJANA KUKAVICA ◽  
TIJANA CVETIĆ ◽  
MILOŠ MOJOVIĆ ◽  
ŽELJKO VUČINIĆ
Keyword(s):  
Cell Wall ◽  
Ascorbic Acid ◽  
Epr Spectroscopy ◽  
Root Cell ◽  
Pea Root ◽  
Oxidative Processes ◽  
Root Cell Wall

scholarly journals The Endoplasmic Reticulum and the Golgi Structures in Maize Root Cells

1959 ◽  
Vol 5 (3) ◽  
pp. 501-506 ◽  
Author(s):  
W. Gordon Whaley ◽  
Hilton H. Mollenhauer ◽  
Joyce E. Kephart
Keyword(s):  
Endoplasmic Reticulum ◽  
Cell Wall ◽  
Electron Microscope ◽  
Nuclear Envelope ◽  
Epoxy Resin ◽  
Maize Root ◽  
Root Tips ◽  
Animal Cells ◽  
Root Cells ◽  
Vesicular Structure

Maize root tips were fixed in potassium permanganate, embedded in epoxy resin, sectioned to show silver interference color, and studied with the electron microscope. All the cells were seen to contain an endoplasmic reticulum and apparently independent Golgi structures. The endoplasmic reticulum is demonstrated as a membrane-bounded, vesicular structure comparable in many aspects to that of several types of animal cells. With the treatment used here the membranes appear smooth surfaced. The endoplasmic reticulum is continuous with the nuclear envelope and, by contact at least, with structures passing through the cell wall. The nuclear envelope is characterized by discontinuities, as previously reported for animal cells. The reticula of adjacent cells seem to be in contact at or through the plasmodesmata. Because of these contacts the endoplasmic reticulum of a given cell appears to be part of an intercellular system. The Golgi structures appear as stacks of platelet-vesicles which apparently may, under certain conditions, produce small vesicles around their edges. Their form changes markedly with development of the cell.

scholarly journals The role of silicon in cadmium alleviation by rice root cell wall retention and vacuole compartmentalization under different durations of Cd exposure

2021 ◽  
Vol 226 ◽  
pp. 112810
Author(s):  
Wei Wei ◽  
Hua Peng ◽  
Yunhe Xie ◽  
Xin Wang ◽  
Rui Huang ◽  
...  
Keyword(s):  
Cell Wall ◽  
Root Cell ◽  
Rice Root ◽  
Cd Exposure ◽  
Root Cell Wall

Contributions of root cell wall polysaccharides to Cu sequestration in castor (Ricinus communis L.) exposed to different Cu stresses

2020 ◽  
Vol 88 ◽  
pp. 209-216
Author(s):  
Chao Ren ◽  
Yongbo Qi ◽  
Guoyong Huang ◽  
Shiyuan Yao ◽  
Jinwei You ◽  
...  
Keyword(s):  
Cell Wall ◽  
Ricinus Communis ◽  
Root Cell ◽  
Cell Wall Polysaccharides ◽  
Ricinus Communis L ◽  
Root Cell Wall

Lead-induced oxidative stress triggers root cell wall remodeling and increases lead absorption through esterification of cell wall polysaccharide

2020 ◽  
Vol 385 ◽  
pp. 121524 ◽  
Author(s):  
Jing Zhang ◽  
Yiguang Qian ◽  
Zhongbing Chen ◽  
Maurice Amee ◽  
Hong Niu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Wall ◽  
Root Cell ◽  
Cell Wall Polysaccharide ◽  
Lead Absorption ◽  
Cell Wall Remodeling ◽  
Root Cell Wall

scholarly journals Developmental Modulation of Root Cell Wall Architecture Confers Resistance to an Oomycete Pathogen

2020 ◽  
Vol 30 (21) ◽  
pp. 4165-4176.e5
Author(s):  
Aleksandr Gavrin ◽  
Thomas Rey ◽  
Thomas A. Torode ◽  
Justine Toulotte ◽  
Abhishek Chatterjee ◽  
...  
Keyword(s):  
Cell Wall ◽  
Root Cell ◽  
Oomycete Pathogen ◽  
Developmental Modulation ◽  
Root Cell Wall ◽  
Cell Wall Architecture

Boron alleviates cadmium toxicity in Brassica napus by promoting the chelation of cadmium onto the root cell wall components

2020 ◽  
Vol 728 ◽  
pp. 138833 ◽  
Author(s):  
Xiuwen Wu ◽  
Haixing Song ◽  
Chunyun Guan ◽  
Zhenhua Zhang
Keyword(s):  
Cell Wall ◽  
Brassica Napus ◽  
Cadmium Toxicity ◽  
Root Cell ◽  
Cell Wall Components ◽  
Wall Components ◽  
Root Cell Wall

Aluminum induces oxidative burst, cell wall NADH peroxidase activity, and DNA damage in root cells of Allium cepa L

2012 ◽  
Vol 53 (7) ◽  
pp. 550-560 ◽  
Author(s):  
V. Mohan M. Achary ◽  
Narasimham L. Parinandi ◽  
Brahma B. Panda
Keyword(s):  
Dna Damage ◽  
Cell Wall ◽  
Allium Cepa ◽  
Peroxidase Activity ◽  
Oxidative Burst ◽  
Root Cells ◽  
Allium Cepa L ◽  
Nadh Peroxidase

Oritavancin Retains a High Affinity for a Vancomycin-Resistant Cell-Wall Precursor via Its Bivalent Motifs of Interaction

Biochemistry ◽  
2018 ◽  
Vol 57 (18) ◽  
pp. 2723-2732 ◽  
Author(s):  
Sierra Bowden ◽  
Christine Joseph ◽  
Shengzhuang Tang ◽  
Jayme Cannon ◽  
Emily Francis ◽  
...  
Keyword(s):  
Cell Wall ◽  
Resistant Cell ◽  
High Affinity ◽  
Vancomycin Resistant
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