scholarly journals Dendrimer and dendrimer–conjugate protein complexes and protein coronas

2017 ◽  
Vol 95 (9) ◽  
pp. 903-906 ◽  
Author(s):  
Junjie Chen ◽  
Mark M. Banaszak Holl
Keyword(s):  
Small Molecules ◽  
Protein Complexes ◽  
Polymer Surface ◽  
Tight Binding ◽  
Adsorbed Layer ◽  
Protein Corona ◽  
Corona Formation ◽  
Bio Imaging ◽  
Biological Environment ◽  
Dendrimer Conjugates

Dendrimers and dendrimer conjugates are widely employed for biological applications such as bio-imaging and drug delivery. Understanding the interaction between dendrimers and their biological environment is key to evaluating the efficacy and safety of these materials. Proteins can form an adsorbed layer, termed a “protein corona”, on dendrimers in either a non-specific or specific fashion. A tight-binding, non-exchangeable corona is defined as a “hard” corona, whereas a loosely bound, highly exchangeable corona is called a “soft” corona. Recent research indicates that small molecules conjugated to the polymer surface can induce protein structural change, leading to tighter protein–dendrimer binding and further protein aggregation. This “triggered” corona formation on dendrimer and dendrimer conjugates is reviewed and discussed along with the existing hard or soft corona model. This review describes the triggered corona model to further the understanding of protein corona formation.

scholarly journals Toward the Specificity of Bare Nanomaterial Surfaces for Protein Corona Formation

2021 ◽  
Vol 22 (14) ◽  
pp. 7625
Author(s):  
Fabio Vianello ◽  
Alessandro Cecconello ◽  
Massimiliano Magro
Keyword(s):  
Biological Systems ◽  
Protein Corona ◽  
General Concept ◽  
Interdisciplinary Studies ◽  
Inorganic Materials ◽  
Corona Formation ◽  
Common Opinion ◽  
Biological Environment ◽  
New Generation ◽  
Chemical Groups

Aiming at creating smart nanomaterials for biomedical applications, nanotechnology aspires to develop a new generation of nanomaterials with the ability to recognize different biological components in a complex environment. It is common opinion that nanomaterials must be coated with organic or inorganic layers as a mandatory prerequisite for applications in biological systems. Thus, it is the nanomaterial surface coating that predominantly controls the nanomaterial fate in the biological environment. In the last decades, interdisciplinary studies involving not only life sciences, but all branches of scientific research, provided hints for obtaining uncoated inorganic materials able to interact with biological systems with high complexity and selectivity. Herein, the fragmentary literature on the interactions between bare abiotic materials and biological components is reviewed. Moreover, the most relevant examples of selective binding and the conceptualization of the general principles behind recognition mechanisms were provided. Nanoparticle features, such as crystalline facets, density and distribution of surface chemical groups, and surface roughness and topography were encompassed for deepening the comprehension of the general concept of recognition patterns.

Physico-chemical dynamics of protein corona formation on 3D-bimetallic Au@Pd nanodendrites and its implications on biocompatibility

2021 ◽  
pp. 117329
Author(s):  
Adewale O. Oladipo ◽  
Lesego G. Modibedi ◽  
Solange I.I. Iku ◽  
Karin de Bruyn ◽  
Thabo T.I. Nkambule ◽  
...  
Keyword(s):  
Protein Corona ◽  
Chemical Dynamics ◽  
Physico Chemical ◽  
Corona Formation

scholarly journals Synthetic virus-like particles prepared via protein corona formation enable effective vaccination in an avian model of coronavirus infection

Biomaterials ◽  
2016 ◽  
Vol 106 ◽  
pp. 111-118 ◽  
Author(s):  
Hui-Wen Chen ◽  
Chen-Yu Huang ◽  
Shu-Yi Lin ◽  
Zih-Syun Fang ◽  
Chen-Hsuan Hsu ◽  
...  
Keyword(s):  
Protein Corona ◽  
Virus Like Particles ◽  
Corona Formation ◽  
Coronavirus Infection

scholarly journals Nanoparticle Shape: The Influence of Nanoparticle Shape on Protein Corona Formation (Small 25/2020)

Small ◽  
2020 ◽  
Vol 16 (25) ◽  
pp. 2070141
Author(s):  
Rahul Madathiparambil Visalakshan ◽  
Laura E. González García ◽  
Mercy R. Benzigar ◽  
Arthur Ghazaryan ◽  
Johanna Simon ◽  
...  
Keyword(s):  
Protein Corona ◽  
Nanoparticle Shape ◽  
Corona Formation

scholarly journals Periprotein lipidomes of Saccharomyces cerevisiae provide a flexible environment for conformational changes of membrane proteins

eLife ◽  
2020 ◽  
Vol 9 ◽  
Author(s):  
Joury S van 't Klooster ◽  
Tan-Yun Cheng ◽  
Hendrik R Sikkema ◽  
Aike Jeucken ◽  
Branch Moody ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Membrane Proteins ◽  
Small Molecules ◽  
Conformational Changes ◽  
Direct Detection ◽  
Protein Complexes ◽  
Lateral Diffusion ◽  
Low Ph ◽  
Lipid Particles ◽  
High Degree

Yeast tolerates a low pH and high solvent concentrations. The permeability of the plasma membrane (PM) for small molecules is low and lateral diffusion of proteins is slow. These findings suggest a high degree of lipid order, which raises the question of how membrane proteins function in such an environment. The yeast PM is segregated into the Micro-Compartment-of-Can1 (MCC) and Pma1 (MCP), which have different lipid compositions. We extracted proteins from these microdomains via stoichiometric capture of lipids and proteins in styrene-maleic-acid-lipid-particles (SMALPs). We purified SMALP-lipid-protein complexes by chromatography and quantitatively analyzed periprotein lipids located within the diameter defined by one SMALP. Phospholipid and sterol concentrations are similar for MCC and MCP, but sphingolipids are enriched in MCP. Ergosterol is depleted from this periprotein lipidome, whereas phosphatidylserine is enriched relative to the bulk of the plasma membrane. Direct detection of PM lipids in the 'periprotein space' supports the conclusion that proteins function in the presence of a locally disordered lipid state.

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