Soybean (Glycine max) PHYTOCHROME-INTERACTION FACTOR 1 induced skotomorphogenesis and de-etiolation in Arabidopsis

2020 ◽  
pp. 1-12
Author(s):  
Tianliang Wang ◽  
Shuo Wang ◽  
Jinhao Zhang ◽  
Fan Yan ◽  
Yajing Liu ◽  
...  
Keyword(s):  
Glycine Max ◽  
Light Response ◽  
Chlorophyll Synthesis ◽  
Energy Utilization ◽  
Yeast Two Hybrid ◽  
Etiolated Seedlings ◽  
Photooxidative Damage ◽  
Interaction Factor ◽  
Glycine Max L ◽  
Two Hybrid

Skotomorphogenesis occurs after germination and before excavation in plants. It inhibits excessive absorbed energy in cells and can prevent the lethal photooxidative damage caused by transitioning from skotomorphogenesis to photomorphogenesis for light energy utilization. To investigate the mechanisms underlying photoreactions in soybean [Glycine max (L.) Merr.], we identified and isolated soybean phytochrome-interaction factor 1 (GmPIF1). A yeast two-hybrid (Y2H) assay showed that GmPIF1 interacted with photoactive PHYTOCHROME A (PHYA) and B (PHYB) in both soybean and Arabidopsis (GmPHYA, GmPHYB, AtPHYA, and AtPHYB). To analyze its function, we ectopically over-expressed GmPIF1 in wild type and pif1 mutant Arabidopsis. In etiolated seedlings, GmPIF1 caused hypocotyl elongation, cotyledon closed, apical hooks folded, and less accumulation of protochlorophyllide. In Y2H, GmPIF1 interacted with AtHDA15 that inhibited chlorophyll synthesis under dark conditions. After transition from darkness to white light, GmPIF1 promotes the reduction of photobleaching and induced de-etiolation. Moreover, GmPIF1 inhibited PHYA- and PHYB-mediated seed germination. Our findings increase our understanding of the regulatory network of light response in soybean and provide useful gene resources for soybean breeding in programs and genetics engineering.

scholarly journals Rsc3K, a SMV resistance gene localized on chromosome 2 in soybean cv. Kefeng No.1, interacts with virulence determinant P3

2022 ◽  
Author(s):  
Tongtong Jin ◽  
Jinlong Yin ◽  
Song Xue ◽  
Bowen Li ◽  
Tingxuan Zong ◽  
...  
Keyword(s):  
Soybean Mosaic Virus ◽  
Direct Interaction ◽  
Chromosome 2 ◽  
Internal Deletion ◽  
Yeast Two Hybrid ◽  
Viral Pathogens ◽  
Virulence Determinant ◽  
Glycine Max L ◽  
Two Hybrid ◽  
Avirulent Isolate

Soybean mosaic virus (SMV) is one of the most devastating viral pathogens in Glycine max (L.) Merr (soybean). Twenty-two SMV strains (SC1-SC22) isolated in China were identified based on their responses to ten soybean cultivars. By using the F2-derived F3 (F2:3) and recombinant inbred line (RIL) populations of resistant Soybean cultivar (cv.) Kefeng No.1 × susceptible cv. Nannong 1138-2, we localized the gene mediating resistant to SMV-SC3 strain to a 90 kb interval on chromosome 2 in Kefeng No.1. Bean pod mottle vi-rus (BPMV)-induced gene silencing (VIGS) were used to study the gene function of candidate genes in the mapping interval and revealed that an recombinant gene, later named as Rsc3K, caused by internal deletion of a genomic DNA fragement in Kefeng No.1, is the resistant gene to SMV-SC3. By shuffling genes between avirulent isolate SC3 and avirulent SMV isolate 1129, we found that P3 is the virulence determinant causing resistance on Kefeng No.1. We showed the interaction between Rsc3K and P3 by the yeast two-hybrid (Y2H) and bimolecular fluorescent complementation (BiFC) assays. In conclusion, this study demonstrated that Rsc3K plays a crucial role in resistance of Kefeng No.1 to SMV-SC3 by direct interaction with viral protein P3.

scholarly journals SOMATIC CROSSING OVER IN GLYCINE MAX (L.) MERRILL: MUTAGENICITY OF SODIUM AZIDE AND LACK OF SYNERGISTIC EFFECT WITH CAFFEINE AND MITOMYCIN C

Genetics ◽  
1973 ◽  
Vol 75 (2) ◽  
pp. 265-277
Author(s):  
B K Vig
Keyword(s):  
Glycine Max ◽  
Mitomycin C ◽  
Point Mutations ◽  
Chromosome Breakage ◽  
Chlorophyll Synthesis ◽  
Crossing Over ◽  
Golden Yellow ◽  
Glycine Max L ◽  
Dark Green ◽  
Light Green

ABSTRACT Glycine max (soybean) is one angiosperm which lends itself to the study of somatic crossing over. This is made possible because some varieties have gene combinations Y11Y11, Y11y11 and y11y11 in the segregating populations from Y11y11 plants. The gene in question is responsible for chlorophyll synthesis. The Y11Y11 plants have dark green leaves, Y11y11 are light green and y11y11 plants are golden yellow. The heterozygous plants have dark green, yellow and dark green-yellow (double) spots on the leaves of the untreated control material, whereas the two homozygotes are almost always devoid of somatic sectoring. Application of caffeine, or mitomycin C, to the seeds increased the frequency of double, dark green and yellow spots on the Y11y11 background. Possibly, some dark green or yellow spots originate by failure of one of the two components of what might start as a double spot due to somatic crossing over. The application of NaN3 increases the frequency of dark green or yellow spots, almost exclusively. The two spots increase in equal frequency. The y11y11 plants so treated do not have any light green sectors, but dark green, Y11Y11, plants do develop a few light green or very dark green spots. The data indicate that NaN3 is capable of inducing nondisjunction, but does not cause mutations (at this locus), chromosome fragmentations (segmental losses) or somatic crossing over to an appreciable degree. It has previously been shown that caffeine-induced chromosome rejoining in Vicia faba can be inhibited by treating the roots with NaN3. In the present experiments NaN3 did not affect the processes of somatic crossing over as induced by caffeine or mitomycin C. The effect was additive. This system offers advantages for studying chemical mutagens in that somatic crossing over, point mutations, segmental losses through chromosome breakage and nondisjunction can all be studied in a single treatment to the seeds.

Prevalence of the pit and antipit in the genus Glycine

1987 ◽  
Vol 45 ◽  
pp. 986-987
Author(s):  
R. W. Yaklich ◽  
E. L. Vigil ◽  
W. P. Wergin
Keyword(s):  
Amino Acids ◽  
Endoplasmic Reticulum ◽  
Glycine Max ◽  
Seed Coat ◽  
Cell Types ◽  
Abaxial Surface ◽  
Legume Seed ◽  
Initial Report ◽  
Cone Cells ◽  
Glycine Max L

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.

PENGARUH PEMBERIAN PUPUK ORGANIK CAIR ASAL LIMBAH TERHADAP SERAPAN HARA SERTA PRODUKSI TANAMAN KEDELAI DI LAHAN GAMBUT

2017 ◽  
Vol 2 (02) ◽  
pp. 204-218
Author(s):  
Hendra Saputra ◽  
Intan Sari ◽  
Muhammad Arfah
Keyword(s):  
Glycine Max ◽  
Glycine Max L

Penelitian tentang pengaruh pemberian Pupuk organik cair (POC) asal limbah tumbuhan terhadap serapan hara N dan P serta produksi tanaman kedelai (Glycine max (L) Merrill) di lahan gambut telah dilaksanakan di kampus II Unisi Fakultas Pertanian Jl. Lintas Propinsi Parit 01, Desa Pulau Palas, Kecamatan Tembilahan Hulu, Kabupaten Indragiri Hilir Propinsi Riau. Dimulai dari bulan Agustus sampai bulan Oktober 2013. Tujuan dari penelitian ini untuk mendapatkan POC asal limbah tumbuhan yang terbaik untuk serapan hara N dan P serta produksi tanaman kedelai di lahan gambut. Penelitian ini menggunakan rancangan acak kelompok (RAK) faktor tunggal dengan 7 perlakuan dan 4 ulangan, 2 tanaman dijadikan sampel. Perlakuan dosis POC limbah tanaman pisang dan POC limbah sayur kol yang diberikan yaitu 0 L/Ha, 200 L/Ha, 400 L/Ha dan 600 L/Ha. Parameter pengamatan yaitu : serapan hara N dan P pada fase awal generatif, tinggi tanaman, jumlah bintil akar, polong hampa, produksi perplot, berat 100 biji dan brangkasan kering. Data pengamatan dianalisis dengan sidik ragam (ANOVA) dan dilanjutkan dengan Uji Lanjut Tukey HSD pada taraf 5%. Berdasarkan penelitian yang telah dilaksanakan dapat disimpulkan bahwa pemberian POC asal limbah tumbuhan tidak berpengaruh nyata terhadap serapan hara N dan P, tinggi tanaman, jumlah bintil akar, polong hampa, brangkasan kering tetapi berpengaruh nyata terhadap produksi perplot dan berat 100 biji.

PERTUMBUHAN DAN HASIL SEMBILAN KULTIVAR KEDELAI (Glycine max (L.) Merrrill) DI LAHAN SAWAH

2018 ◽  
Vol 1 (2) ◽  
pp. 96
Author(s):  
Siti Wahyuni ◽  
Umi Trisnaningsih ◽  
Meilina Prasetyo
Keyword(s):  
Glycine Max ◽  
Glycine Max L

COPPER AND ZINC INDUCED CHANGES IN SOYBEAN (Glycine max (L.) Merr.)

1970 ◽  
pp. 09
Author(s):  
K. SANKAR GANESH ◽  
P. SUNDARAMOORTHY
Keyword(s):  
Heavy Metals ◽  
Glycine Max ◽  
Aquatic Environment ◽  
Morphological Traits ◽  
Industrial Activities ◽  
Copper And Zinc ◽  
Glycine Max L ◽  
Zinc Concentrations ◽  
Induced Changes

Heavy metals are one of the most important pollutants released to the aquatic environment by the various industrial activities. The use of these wastewater for irrigation results accumulation of heavy metals in soil and plants. So, the present investigation deals with the various concentrations (0, 5, 10, 25, 50, 100, 200 and 300 mg/l) of copper and zinc on germination studies of soybean. The different concentrations of copper and zinc were used for germination studies. The seedlings were allowed to grow upto seven days. The studied morphological traits increased at 5 mg/l concentration and these parameters are gradually decreased with the increase of copper and zinc concentrations.

Pengaruh Jenis Kemasan terhadap Daya Simpan Benih Kedelai (Glycine max (L.) Merrill) Varietas Anjasmoro

2018 ◽  
Vol 6 (1) ◽  
pp. 21
Author(s):  
Fadilah Ramadhani ◽  
Memen Surahman ◽  
Andri Ernawati
Keyword(s):  
Glycine Max ◽  
Glycine Max L

<p><em>Penelitian ini bertujuan untuk mengetahui pengaruh tiga jenis kemasan yaitu kemasan hermetik, jeriken plastik dan karung </em><em>beralas </em><em>plastik terhadap mutu fisik maupun fisiologis benih kedelai (Glycine max (L.) Merrill) varietas Anjasmoro selama penyimpanan. Penelitian dilaksanakan di Laboratorium Ilmu dan Teknologi Benih, Departemen Agronomi dan Hortikultura, Fakultas Pertanian, Institut Pertanian Bogor pada bulan </em><em>Januari</em><em> sampai </em><em>Juli</em><em> 2016. Rancangan yang digunakan adalah rancangan acak lengkap (RAL) dengan tiga ulangan. Faktor perlakuan yang digunakan adalah jenis kemasan (P) yang terdiri atas tiga taraf yaitu kemasan hermetik (P1), jeriken plastik (P2), dan karung </em><em>beralas </em><em>plastik (P3). Penyimpanan dilakukan selama 6 bulan dan diamati setiap bulan terhadap variabel kadar air, daya berkecambah, indeks vigor, kecepatan tumbuh, keserempakan tumbuh dan bobot kering kecambah normal. Hasil penelitian menunjukkan bahwa perlakuan jenis kemasan tidak memberikan pengaruh yang nyata terhadap mutu fisik maupun fisiologis benih kedelai varietas Anjasmoro selama penyimpanan. Perlakuan jenis kemasan dapat mempertahankan viabilitas dan vigor benih kedelai hingga bulan ke-2 penyimpanan. Hasil tersebut menunjukan bahwa benih kedelai dapat disimpan selama 4 bulan setelah panen.</em></p>

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