Determination of Isopropyl Alcohol in Fish Protein Concentrate by Solvent Extraction and Gas–Liquid Chromatography

1967 ◽  
Vol 24 (7) ◽  
pp. 1521-1529 ◽  
Author(s):  
R. G. Ackman ◽  
H. J. Hingley ◽  
H. E. Power
Keyword(s):  
Chromatographic Analysis ◽  
Methyl Acetate ◽  
Isopropyl Alcohol ◽  
Gas Liquid Chromatography ◽  
Protein Concentrate ◽  
Fish Protein ◽  
Protein Concentrates ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

The isopropyl alcohol residues in fish protein concentrates prepared with this solvent can be determined by a hot extraction technique employing methyl acetate as the solvent and gas–liquid chromatographic analysis of the extract. Studies on vacuum stripping of fish protein concentrates and analysis of various samples suggest that the isopropyl alcohol is trapped mechanically inside particles because of formation of an impervious shell during drying.

scholarly journals Simultaneous Liquid Chromatographic Analysis of the β-Lactam Antibiotics Cefazolin, Cefadroxil, Cephalexin, Ampicillin, and Cephradine in Solution

1998 ◽  
Vol 81 (2) ◽  
pp. 381-385 ◽  
Author(s):  
Shawky A Farag
Keyword(s):  
Chromatographic Analysis ◽  
Room Temperature ◽  
Isopropyl Alcohol ◽  
Reversed Phase ◽  
Chromatographic Retention ◽  
Liquid Chromatographic Method ◽  
Liquid Chromatographic Analysis ◽  
Liquid Chromatographic

abstract A liquid chromatographic method was developed for the determination of nanogram quantities of 5 broad-spectrum structurally related β-lactam antibiotics (cefazolin, cefadroxil, cephalexin, cephradine, and ampicillin) in solution. The method uses a C18 reversed-phase column, UV absorption (240 nm) detection, and an aqueous mobile phase containing isopropyl alcohol and acetic acid. Relative resolution between the antibiotic peaks ranged from 1.7 to 5.9 for all peaks. Chromatographic retention times were 2.97, 3.92, 4.57, 5.37, and 6.56 min for cefazolin, cefadroxil, cephalexin, ampicillin, and cephradine, respectively. Accuracy, precision, linearity, and long term analytical reproducibility were determined by statistical analysis. Use of the proposed method to evaluate the degradation of cephradine solutions stored at room temperature illustrated its potential as a stability-indicating assay.

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