Concentrations of Leucine Aminonaphthylamidase (LAN) and Soluble Protein in Tissues of Rainbow Trout, Salmo gairdneri

1980 ◽  
Vol 37 (1) ◽  
pp. 116-120 ◽  
Author(s):  
Gerald R. Bouck
Keyword(s):  
Rainbow Trout ◽  
Blood Plasma ◽  
Plasma Protein ◽  
Soluble Protein ◽  
Discrete Distribution ◽  
Salmo Gairdneri ◽  
Similar Amount ◽  
Plasma Enzymes ◽  
Tissue Equivalent

The levels of soluble protein (Biuret-reactive) and levels of leucine aminonaphthylamidase (LAN, 3.4.1.1) were determined quantitatively in extracts of 10 organs from rainbow trout (Salmo gairdneri). Soluble protein had a very discrete distribution and averaged 5.2 times more concentrated in liver than in blood plasma (3.5 g/100 mL). LAN was present in the extract of every tissue except saline-washed brain. LAN activity was highest in hindgut, 36 more times more concentrated than in the plasma of healthy fish. Wasting disorders such as caused by stress, would likely increase the level of necrosis which in turn would increase the amount of LAN in plasma. I propose that a "tissue equivalent" unit of LAN to provide a means of relating increased LAN in plasma to an amount of liver destruction in vitro that would render a similar amount of LAN.Key words: plasma enzymes, stress, plasma protein, rainbow trout, leucine aminonaphthylamidase, fish physiology

scholarly journals Some effects of vitamin E and selenium deprivation on tissue enzyme levels and indices of tissue peroxidation in rainbow trout (Salmo gairdneri)

1985 ◽  
Vol 53 (1) ◽  
pp. 149-157 ◽  
Author(s):  
J. G. Bell ◽  
C. B. Cowey ◽  
J. W. Adron ◽  
Aileen M. Shanks
Keyword(s):  
Rainbow Trout ◽  
Vitamin E ◽  
Peroxidase Activity ◽  
Cumene Hydroperoxide ◽  
Dietary Vitamin ◽  
Salmo Gairdneri ◽  
Deficient Diet ◽  
Exudative Diathesis ◽  
Malondialdehyde Formation

1. Duplicate groups of rainbow trout (Salrno gairdnert) (mean weight 11 g) were given for 40 weeks one of four partially purified diets that were either adequate or low in selenium or vitamin E or both.2. Weight gains of trout given the dually deficient diet were significantly lower than those of trout given a complete diet or a diet deficient in Se. No mortalities occurred and the only pathology seen was exudative diathesis in the dually deficient trout.3. There was significant interaction between the two nutrients both with respect to packed cell volume and to malondialdehyde formation in the in vitro NADPH-dependent microsomal lipid peroxidation system.4. Tissue levels of vitamin E and Se decreased to very low levels in trout given diets lacking these nutrients. For plasma there was a significant effect of dietary vitamin E on Se concentration.5. Glutathione (GSH) peroxidase (EC 1. 1 1. 1.9) activity in liver and plasma was significantly lower in trout receiving low dietary Se but was independent of vitamin E intake. The ratios of hepatic GSH peroxidase activity measured with cumene hydroperoxide and hydrogen peroxide were the same for all treatments. This confirms the absence of a Se-independent GSH peroxidase activity in trout liver.6. Se deficiency did not lead to any compensatory increase in hepatic GSH transferase (EC 2. 5. 1. 18) activity; values were essentially the same in all treatments.7. Plasma pyruvate kinase (EC 2. 7. 1.40) activity increased significantly in the trout deficient in both nutrients. This was thought to be due to leakage of the enzyme from the muscle and may be indicative of incipient (subclinical) muscle damage.

In vitro mitogenesis of peripheral blood lymphocytes from rainbow trout (Salmo gairdneri)

1988 ◽  
Vol 89 (1) ◽  
pp. 25-35 ◽  
Author(s):  
Donald E. Tillitt ◽  
John P. Giesy ◽  
Paul O. Fromm
Keyword(s):  
Rainbow Trout ◽  
Peripheral Blood ◽  
Peripheral Blood Lymphocytes ◽  
Salmo Gairdneri ◽  
Blood Lymphocytes

Steroid biosynthesis in vitro by testes of rainbow trout, Salmo gairdneri

1967 ◽  
Vol 8 (2) ◽  
pp. 305-313 ◽  
Author(s):  
Ryoichi Arai ◽  
Bun-Ichi Tamaoki
Keyword(s):  
Rainbow Trout ◽  
Salmo Gairdneri ◽  
Steroid Biosynthesis

Characterization and Subcellular Localization of Leucine Aminonaphthylamidase (LAN) in Rainbow Trout (Salmo gairdneri)

1975 ◽  
Vol 32 (8) ◽  
pp. 1289-1295 ◽  
Author(s):  
G. R. Bouck ◽  
P. W. Schneider Jr. ◽  
Janet Jacobson ◽  
R. C. Ball
Keyword(s):  
Enzyme Activity ◽  
Rainbow Trout ◽  
Reaction Time ◽  
Blood Plasma ◽  
Substrate Concentration ◽  
Room Temperature ◽  
Diagnostic Value ◽  
Inhibitory Effect ◽  
Salmo Gairdneri ◽  
Body Temperatures

LAN analyses appear to have diagnostic value in fish pathobiology and studies were undertaken to determine optima for substrate concentration, pH, reaction time, temperature, and buffer ions. Citrate ion did not inhibit LAN at anticoagulant levels, but cyanide, pyrophosphate, and EDTA had an inhibitory effect. Storage of samples at —10 and 1 C resulted in small but significant reductions of LAN activity, while at room temperature enzyme activity was rapidly lost. LAN activity was distributed among liver fractions as follows: microsomes, 12%; mitochondria, 9%; cellular sap, 37%; other, 50%. Three isozymes of LAN were found. Blood plasma contained significant amounts of LAN activity which was significantly higher in cold- than in warm-acclimated fish. However, these LAN levels were comparable when their activity was extrapolated to body temperatures.

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