Determining Continent of Origin of Chum Salmon (Oncorhynchus keta) Using Genetic Stock Identification Techniques: Status of Allozyme Baseline in Asia

1994 ◽  
Vol 51 (S1) ◽  
pp. 95-113 ◽  
Author(s):  
Gary A. Winans ◽  
Paul B. Aebersold ◽  
Shigehiko Urawa ◽  
Nataly V. Varnavskaya
Keyword(s):  
Chum Salmon ◽  
Principal Component ◽  
Allele Frequencies ◽  
Oncorhynchus Keta ◽  
Stock Identification ◽  
Genetic Stock ◽  
Data Set ◽  
Chum Salmon Oncorhynchus Keta ◽  
Genetic Stock Identification ◽  
Allozyme Loci

A three-agency program was initiated in 1989 to develop a new multilocus genetic baseline for chum salmon (Oncorhynchus keta) in Japan and Russia for use in stock identification; allele frequencies at 77 allozyme loci are reported in 38 samples covering most of its north–south limits of distribution in Asia. In a 62-locus data set for 17 Japanese and 12 Russian samples, average heterozygosity ranged from 0.066 to 0.087 (mean 0.079) and the average number of P0.95 and P0.99 loci was 14 and 26, respectively. Tests of year-to-year variation in allele frequencies were not significant at five of six locations. For the P0.95 loci, FST values ranged from 0.007 (sMDH-B1*) to 0.154 (mAAT-2*) and averaged 0.038. A clear distinction between Russian and Japanese samples was observed at Nei's D = 0.006, and genetic differentiation generally followed a regional pattern within each country. Principal component analysis of the P0.95 loci revealed a large difference between Japanese and Russian samples. Four loci (sAAT-1,2*, mAAT-2*, LDH-A1*, and PEPLT*) had high loadings on the first two principal components. Analyses of a simulated fishery with 200 fish revealed a high degree of precision in estimating contributions to seven population groups and to country of origin.

Biochemical Genetic Stock Identification of Chum Salmon (Oncorhynchus keta) in Southern British Columbia

1985 ◽  
Vol 42 (3) ◽  
pp. 437-448 ◽  
Author(s):  
Terry D. Beacham ◽  
Ruth E. Withler ◽  
Allan P. Gould
Keyword(s):  
British Columbia ◽  
Chum Salmon ◽  
Vancouver Island ◽  
Oncorhynchus Keta ◽  
Significant Heterogeneity ◽  
Stock Identification ◽  
Fraser River ◽  
Genetic Stock ◽  
Allelic Frequencies ◽  
Chum Salmon Oncorhynchus Keta

We used electrophoresis to examine genetic variability of 33 chum salmon (Oncorhynchus keta) stocks in southern British Columbia and used differences in genotypic frequencies among these stocks for estimating stock compositions in a weekly fishery in upper Johnstone Strait. Seven polymorphic loci were used for stock identification. Chum salmon from the Fraser River, Bute and Toba inlets, Vancouver Island, and the southern Mainland had significantly different allelic frequencies, but there was also significant heterogeneity in allelic frequencies at some loci within each region. Allelic frequencies were generally stable over a 2-yr period in nine stocks for which consecutive annual sampling was conducted. There was no significant two-locus linkage disequilibrium for the chum salmon stocks surveyed. Cluster analysis indicated that Fraser River and Bute and Toba Inlet stocks were distinctive, but Vancouver Island and Mainland stocks were not. The timing and relative abundance of Fraser River chum salmon in upper Johnstone Strait as estimated by electrophoretic analysis was confirmed by an in-river test fishery in the Fraser River.

Biochemical Genetic Survey and Stock Identification of Chum Salmon (Oncorhynchus keta) in British Columbia

1987 ◽  
Vol 44 (10) ◽  
pp. 1702-1713 ◽  
Author(s):  
T. D. Beacham ◽  
A. P. Gould ◽  
R. E. Withler ◽  
C. B. Murray ◽  
L. W. Barner
Keyword(s):  
British Columbia ◽  
Chum Salmon ◽  
Electrophoretic Analysis ◽  
South Coast ◽  
Oncorhynchus Keta ◽  
Stock Identification ◽  
Fraser River ◽  
Genetic Method ◽  
Chum Salmon Oncorhynchus Keta ◽  
Queen Charlotte Islands

We examined genetic variability using electrophoretic analysis of 83 chum salmon (Oncorhynchus keta) stocks in British Columbia and used regional differences in genotypic frequencies of seven polymorphic loci to estimate stock compositions in a number of commercial and experimental test fisheries. Chum salmon from five regions could be discriminated: Queen Charlotte islands, north and central coast, west coast of Vancouver Island, the Fraser River and its tributaries, and the south coast (rivers draining into Johnstone Strait and the Strait of Georgia). Allelic frequencies from 33 stocks were generally stable over a period of 2 yr or more. South coast chum salmon was the dominant run migrating through upper Johnstone Strait in October 1982–85. The genetic method of stock identification provided managers with the relative proportions of Fraser River and other groups of chum salmon in a number of fisheries and allowed managers to regulate more effectively the exploitation rate of Fraser River chum salmon.

scholarly journals Development of molecular markers for genetic stock identification of chum salmon

2002 ◽  
Vol 68 (sup1) ◽  
pp. 353-356 ◽  
Author(s):  
SYUITI ABE ◽  
SHUNPEI SATO ◽  
HIROYUKI KOJIMA ◽  
JUNKO ANDO ◽  
HIRONORI ANDO ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Chum Salmon ◽  
Stock Identification ◽  
Genetic Stock ◽  
Genetic Stock Identification

Population structure and stock identification of chum salmon (Oncorhynchus keta) from British Columbia determined with microsatellite DNA variation

2008 ◽  
Vol 86 (9) ◽  
pp. 1002-1014 ◽  
Author(s):  
Terry D. Beacham ◽  
Brian Spilsted ◽  
Khai D. Le ◽  
Michael Wetklo
Keyword(s):  
British Columbia ◽  
Population Structure ◽  
Chum Salmon ◽  
Oncorhynchus Keta ◽  
Stock Identification ◽  
Dna Variation ◽  
Coastal Fisheries ◽  
Chum Salmon Oncorhynchus Keta ◽  
Regional Estimates ◽  
Mixed Stock

Variation at 14 microsatellite loci was surveyed in 205 populations of chum salmon ( Oncorhynchus keta (Walbaum in Artedi, 1792)) from British Columbia to determine population structure and the possible application of microsatellites to estimate stock composition of chum salmon in mixed-stock fisheries. The genetic differentiation index (FST) over all populations and loci was 0.016, with individual locus values ranging from 0.006 to 0.059. Sixteen regional stocks were defined in British Columbia for stock identification applications. Analysis of simulated fishery samples suggested that accurate and precise regional estimates of stock composition should be produced when the microsatellites were used to estimate stock compositions. The main stocks that constitute the October 2007 samples of migrating chum salmon through Johnstone Strait in southern British Columbia were Fraser River (45%–64%), southern British Columbia mainland (22%), and east coast Vancouver Island (13%–28%), within the range of those to be expected in samples from Johnstone Strait. Microsatellites have the ability to provide fine-scale resolution of stock composition in British Columbia coastal fisheries.

scholarly journals Stock identification of chum salmon Oncorhynchus keta from their maturity and scale chraracters.

1989 ◽  
Vol 55 (4) ◽  
pp. 651-656 ◽  
Author(s):  
Yukimasa Ishida ◽  
Soto Ito ◽  
Kenji Takagi
Keyword(s):  
Chum Salmon ◽  
Oncorhynchus Keta ◽  
Stock Identification ◽  
Chum Salmon Oncorhynchus Keta

DNA and allozyme markers provide concordant estimates of population differentiation: analyses of U.S. and Canadian populations of Yukon River fall-run chum salmon (Oncorhynchus keta)

1998 ◽  
Vol 55 (7) ◽  
pp. 1748-1758 ◽  
Author(s):  
Kim T Scribner ◽  
Penelope A Crane ◽  
William J Spearman ◽  
Lisa W Seeb
Keyword(s):  
Genetic Markers ◽  
Population Differentiation ◽  
Chum Salmon ◽  
Allele Frequencies ◽  
Border Region ◽  
Oncorhynchus Keta ◽  
Mitochondrial Haplotype ◽  
Chum Salmon Oncorhynchus Keta ◽  
Yukon River ◽  
Mixed Stock

Although the number of genetic markers available for fisheries research has steadily increased in recent years, there is limited information on their relative utility. In this study, we compared the preformance of different "classes" of genetic markers (mitochondrial DNA (mtDNA), nuclear DNA (nDNA), and allozymes) in terms of estimating levels and partitioning of genetic variation and of the relative accuracy and precision in estimating population allocations to mixed-stock fisheries. Individuals from eight populations of fall-run chum salmon (Oncorhynchus keta) from the Yukon River in Alaska and Canada were assayed at 25 loci. Significant differences in mitochondrial haplotype and nuclear allele frequencies were observed among five drainages. Populations from the U.S.-Canada border region were not clearly distinguishable based on multilocus allele frequencies. Although estimates of total genetic diversities were higher for the DNA loci (Ht = 0.592 and h = 0.647 for nDNA and mtDNA, respectively) compared with protein allozymes (Ht = 0.250), estimates of the extent of population differentiation were highly concordant across marker classes (mean theta = 0.010, 0.011, and 0.016 for allozymes, nDNA, and mtDNA, respectively). Simulations of mixed-stock fisheries composed of varying contributions of U.S. and Canadian populations revealed a consistent bias for overallocation of Canadian stocks when expected Canadian contributions varied from 0 to 40%, due primarily to misallocations among genetically similar border populations. No single marker class is superior for differentiating populations of this species at the spatial scale examined.

Genetic stock identification of chum salmon in the Bering Sea and North Pacific Ocean using mitochondrial DNA microarray

Aquaculture ◽  
2007 ◽  
Vol 272 ◽  
pp. S238-S239
Author(s):  
S. Abe ◽  
S. Moriya ◽  
S. Sato ◽  
T. Azumaya ◽  
O. Suzuki ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Microarray ◽  
Bering Sea ◽  
North Pacific ◽  
Chum Salmon ◽  
North Pacific Ocean ◽  
Stock Identification ◽  
Genetic Stock ◽  
Genetic Stock Identification ◽  
The Bering Sea

Genetic stock identification of overwintering chum salmon in the North Pacific Ocean

2011 ◽  
Vol 94 (4) ◽  
pp. 663-668 ◽  
Author(s):  
W. Tyler McCraney ◽  
Edward V. Farley ◽  
Christine M. Kondzela ◽  
Svetlana V. Naydenko ◽  
Alexander N. Starovoytov ◽  
...  
Keyword(s):  
Pacific Ocean ◽  
North Pacific ◽  
Chum Salmon ◽  
North Pacific Ocean ◽  
Stock Identification ◽  
Genetic Stock ◽  
The North ◽  
Genetic Stock Identification ◽  
The North Pacific

Genetic Stock Identification of Chum Salmon in the Bering Sea and North Pacific Ocean Using Mitochondrial DNA Microarray

2006 ◽  
Vol 9 (2) ◽  
pp. 179-191 ◽  
Author(s):  
Shogo Moriya ◽  
Shunpei Sato ◽  
Tomonori Azumaya ◽  
Osamu Suzuki ◽  
Shigehiko Urawa ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Dna Microarray ◽  
Bering Sea ◽  
North Pacific ◽  
Chum Salmon ◽  
North Pacific Ocean ◽  
Stock Identification ◽  
Genetic Stock ◽  
Genetic Stock Identification ◽  
The Bering Sea
Sign in / Sign up

Export Citation Format

Share Document