An SSR-based linkage map of yardlong bean (Vigna unguiculata (L.) Walp. subsp. unguiculata Sesquipedalis Group) and QTL analysis of pod length

Genome ◽  
2012 ◽  
Vol 55 (2) ◽  
pp. 81-92 ◽  
Author(s):  
Alisa Kongjaimun ◽  
Akito Kaga ◽  
Norihiko Tomooka ◽  
Prakit Somta ◽  
Takehiko Shimizu ◽  
...  
Keyword(s):  
Linkage Map ◽  
Segregation Distortion ◽  
Vigna Unguiculata ◽  
Length Variation ◽  
Molecular Linkage Map ◽  
Vigna Species ◽  
Vigna Umbellata ◽  
Yardlong Bean ◽  
Pod Length ◽  
Simple Sequence

Yardlong bean (Vigna unguiculata (L.) Walp. subsp. unguiculata Sesquipedalis Group) (2n = 2x = 22) is one of the most important vegetable legumes of Asia. The objectives of this study were to develop a genetic linkage map of yardlong bean using SSR makers from related Vigna species and to identify QTLs for pod length. The map was constructed from 226 simple sequence repeat (SSR) markers from cowpea (Vigna unguiculata (L.) Walp. subsp. unguiculata Unguiculata Group), azuki bean (Vigna angularis (Willd.) Ohwi & Ohashi), and mungbean (Vigna radiata (L.) Wilczek) in a BC1F1 ((JP81610 × TVnu457) × JP81610) population derived from the cross between yardlong bean accession JP81610 and wild cowpea (Vigna unguiculata subsp. unguiculata var. spontanea) accession TVnu457. The markers were clustered into 11 linkage groups (LGs) spanning 852.4 cM in total length with a mean distance between adjacent markers of 3.96 cM. All markers on LG11 showed segregation distortion towards the homozygous yardlong bean JP81610 genotype. The markers on LG11 were also distorted in the rice bean (Vigna umbellata (Thunb.) Ohwi & Ohashi) map, suggesting the presence of common segregation distortion factors in Vigna species on this LG. One major and six minor QTLs were identified for pod length variation between yardlong bean and wild cowpea. Using flanking markers, six of the seven QTLs were confirmed in an F2 population of JP81610 × TVnu457. The molecular linkage map developed and markers linked to pod length QTLs would be potentially useful for yardlong bean and cowpea breeding.

Effect of plant densities and different environments on productivity and profitability of Yardlong bean (Vigna unguiculata sub sp. sesquipedalis)

2018 ◽  
Author(s):  
M. Manjesh ◽  
Nagarajappa Adivappar ◽  
V. Srinivasa ◽  
G. K. Girijesh ◽  
Sharanabasappa .
Keyword(s):  
Plant Height ◽  
Vigna Unguiculata ◽  
Production Economics ◽  
Higher Plant ◽  
Pod Yield ◽  
Plant Densities ◽  
Yardlong Bean ◽  
Benefit Cost ◽  
Pod Length ◽  
Plot Design

The present investigation was carried out during Kharif 2016 at ZAHRS, Navile, Shivamogga. The experiment was laid out in split plot design with three replications. There were 24 treatment combinations comprised of three different environments (polyhouse, shadenet and open field) and eight spacing treatments (45 cm x 30 cm, 45 cm x 45 cm, 45 cm x 60 cm, 45 cm x 75 cm, 60 cm x 30 cm, 60 cm x 45 cm, 60 cm x 60 cm, 60 cm x 75 cm). The obtained results showed that all the characters were markedly affected by different environments and spacing. Among different environments significantly higher plant height (325.17cm), number of primary branches (12.46), number of pods per plant (21.66), pod length (70.67 cm), pod girth (3.73 cm), pod weight per plant (31.13 g), yield per plant (458.21 g) and higher pod yield per 1000 m2 (1605.99 kg) were recorded in polyhouse. Among spacing treatments significantly higher number of primary branches (12.91), pods per plant (22.30), pod length (71.73 cm), pod girth (3.94 cm), pod weight per plant (33.11g) and pod yield per plant (547.86 g) were recorded with wider spacing of 60 cm x 75 cm. Highest plant height (294.29 cm) and yield per 1000 m2 (1791.68 kg) were recorded with the closer spacing of 45 cm x 30 cm. The interaction of different environments and spacing were found to be statistically non-significant for all the traits. The evaluation of production economics revealed that growing of Yardlong bean in open condition with 45 cm x 30 cm spacing was most remunerative option with a highest benefit: cost of 3.14.

Genetic diversity and population structure of pencil yam (Vigna lanceolata) (Phaseoleae, Fabaceae), a wild herbaceous legume endemic to Australia, revealed by microsatellite markers

Botany ◽  
2015 ◽  
Vol 93 (3) ◽  
pp. 183-191 ◽  
Author(s):  
Phakchana Nubankoh ◽  
Sarocha Pimtong ◽  
Prakit Somta ◽  
Sujinna Dachapak ◽  
Peerasak Srinives
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Diversity ◽  
Taxonomic Revision ◽  
Neighbor Joining ◽  
Complex Species ◽  
Vigna Species ◽  
Vigna Umbellata ◽  
Its Gene ◽  
Simple Sequence

Pencil yam (Vigna lanceolata Benth.) (Phaseoleae, Fabaceae) is a herbaceous legume endemic to Australia. A previous morphological study suggested that pencil yam is a complex species of two or more related taxa with seven distinct morphological types (morphotypes) and, thus, taxonomic revision is necessary. In this study, we assessed genetic diversity and determined the genetic structure of a pencil yam collection of 62 accessions from seven morphotypes using 18 microsatellite (simple sequence repeat) markers with the aim to provide information for taxonomic study. In total, 138 alleles were detected with a mean of 7.67 alleles per locus. Polymorphism information content per marker varied between 0.06 and 0.90 with a mean of 0.61, while the overall gene diversity was 0.62. Bayesian clustering, principal coordinate, and neighbor-joining analyses consistently revealed that these accessions are grouped into two subpopulations with difference in number of alleles, allelic richness, and gene diversity. The population structure was not related to either morphotype or geographical origin. Gene diversity of V. lanceolata was higher than that of wild Vigna radiata (L.) Wilczek and wild Vigna umbellata (Thunb.) Ohwi & Ohashi, comparable with that of wild Vigna mungo (L.) Hepper, Vigna exilis Tateishi & Maxted, and Vigna grandiflora (Prain) Tateishi & Maxted, and lower than that of wild Vigna angularis (Willd.) Ohwi & Ohashi. These results indicated that the taxonomy of V. lanceolata should be revised and that its gene diversity was moderate compared with the other wild Vigna species.

scholarly journals High levels of segregation distortion in the molecular linkage map of bread wheat representing the West Asia and North Africa region

2016 ◽  
Vol 40 ◽  
pp. 352-364 ◽  
Author(s):  
Faheem Shehzad BALOCH ◽  
Ahmad ALSALEH ◽  
Enver Ersoy ANDEDEN ◽  
Rüştü HATİPOĞLU ◽  
Miloudi NACHIT ◽  
...  
Keyword(s):  
Linkage Map ◽  
Bread Wheat ◽  
Segregation Distortion ◽  
North Africa ◽  
The West ◽  
Molecular Linkage Map

scholarly journals Genetic variability, Heritability and Genetic advance studies in Yardlong bean (Vigna unguiculata spp. sesquipedalis) genotypes

2021 ◽  
Vol 23 (2) ◽  
pp. 215-218
Author(s):  
K RAMKUMAR ◽  
Keyword(s):  
Genetic Variability ◽  
Field Experiment ◽  
Vigna Unguiculata ◽  
Coefficient Of Variation ◽  
Genetic Advance ◽  
Pod Yield ◽  
High Heritability ◽  
Yardlong Bean ◽  
The Mean ◽  
Pod Length

A field experiment was carried out at farmer field in Pudukurapettai village, Virudhachalam, Tamilnadu during 2019 to study genetic variability, heritability and genetic advance expressed as per cent of mean for yield and yield attributing characters in thirty three genotypes of yardlong bean. The mean performance recorded on days to first flowering was in the range of (46.78 to 57.33 days), vine length (196.76 to 386.32 cm), days to first picking (57.33 to 67.53 days), pod length (14.18 to 60.08 cm), pod width (1.76 to 3.43 cm), fresh pod weight (171.43 to 995.45 g), number of pods per plant (26.53 to 56.40), number of picking (10.00 to 14.20) and pod yield per plant (171.43 to 995.45 g). The genotypic and phenotypic coefficient of variation showed higher value for pod yield per plant (46.69 % and 46.70 %), fresh pod weight (42.27 and 42.28 %) and pod length (41.66 and 41.66 %). High heritability was observed for the all nine characters. Genetic advance as per cent of mean was higher for pod yield per plant (96.18 %), fresh pod weight (87.08 %), pod length (85.81 %), vine length (36.93 %), pod width (34.16 %) and number of pods per plant (30.04 %).

scholarly journals Inheritance and linkage analysis of co-dominant SSR markers on the Z chromosome of the silkworm (Bombyx mori L.)

2008 ◽  
Vol 90 (2) ◽  
pp. 151-156 ◽  
Author(s):  
XUE-XIA MIAO ◽  
WEI-HUA LI ◽  
MU-WANG LI ◽  
YUN-PO ZHAO ◽  
XIAN-RU GUO ◽  
...  
Keyword(s):  
Linkage Map ◽  
Bombyx Mori ◽  
Ssr Markers ◽  
Positional Cloning ◽  
Z Chromosome ◽  
Pcr Marker ◽  
Backcross Population ◽  
In The Beginning ◽  
Simple Sequence ◽  
Visible Mutation

SummaryMicrosatellites or simple sequence repeats (SSRs) are co-dominant molecular markers. When we used fluorescent SSR markers to construct a linkage map for the female heterogametic silkworm (Bombyx mori, ZW), we found that some loci did not segregate in a Mendelian ratio of 1:1 in a backcross population. These loci segregated in a 3:1 ratio of single bands compared with double bands. Further examination of band patterns indicated that three types of SSR bands were present: two homozygotes and one heterozygote. In the beginning, we considered to discard these markers. By scoring male and female F1 individuals, we confirmed that these loci were located on the Z chromosome. Using the sex-linked visible mutation sch (K05) and its wild-type (C108), we constructed an F1 male backcross (BC1M) mapping population. The combination of sch backcross and SSR data enabled us to map the SSR markers to the Z chromosome. By adjusting input parameters based on these data, we were able to use Mapmaker software to construct a linkage map. This strategy takes advantage of co-dominant markers for positional cloning of genes on the Z chromosome. We localized sch to the Z chromosome relative to six SSR markers and one PCR marker, covering a total of 76·1 cM. The sch mutation is an important sex-linked visible mutation widely used in breeding of commercial silkworms (e.g. male silkworm selection rearing). Localization of the sch gene may prove helpful in cloning the gene and developing strains for marker-assisted selection in silkworm breeding.

Development of simple sequence repeat markers and construction of a high-density linkage map of Capsicum annuum

2013 ◽  
Vol 31 (4) ◽  
pp. 909-920 ◽  
Author(s):  
Toru Sugita ◽  
Yukari Semi ◽  
Hiromasa Sawada ◽  
Yumi Utoyama ◽  
Yuko Hosomi ◽  
...  
Keyword(s):  
Linkage Map ◽  
Capsicum Annuum ◽  
Simple Sequence Repeat ◽  
High Density ◽  
Sequence Repeat ◽  
Simple Sequence Repeat Markers ◽  
High Density Linkage Map ◽  
Simple Sequence
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