EFFECTS OF DIETHYL SULPHATE ON SEED GERMINATION AND SEEDLING GROWTH OF NICOTIANA TABACUM

1969 ◽  
Vol 11 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Bronius Povilaitis

Seeds of three flue-cured tobacco (Nicotiana tabacum L.) varieties, Hicks Broadleaf, Yellow Gold, and Canadel, were soaked for 1, 2, 3 or 5 hours in a saturated solution of diethyl sulphate, a chemical mutagenic agent, to increase variability of plant characteristics. Diethyl sulphate had a detrimental effect on both extent and rate of germination; each decreased with length of soaking period. Seedlings from treated seed grew more slowly than controls.

Author(s):  
Monika Agacka-Mołdoch ◽  
Mian Abdur Rehman Arif ◽  
Ulrike Lohwasser ◽  
Teresa Doroszewska ◽  
Ramsey S. Lewis ◽  
...  

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.


2018 ◽  
Vol 94 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Fabiana de Lima Abrantes ◽  
Alessandra Ferreira Ribas ◽  
Luiz Gonzaga Esteves Vieira ◽  
Nelson Barbosa Machado-Neto ◽  
Ceci Castilho Custódio

2012 ◽  
Vol 10 (1) ◽  
pp. 15-20 ◽  
Author(s):  
R Islam ◽  
A Mukherjee ◽  
M Hossin

The experiment was conducted in the Agronomy Laboratory of Agrotechnology Discipline, Khulna University, Khulna from 29th August to 17th September, 2009. The experiment was laid out in a Completely Randomized Design (CRD) with two factors and three replications. The aim of the experiment was to study the efficacy of three osmopriming agents viz ., NaCl, KC1 and CaCb with control on seed germination and seedling growth of three T. aman rice varities viz., BRRI dhan40, BRRI dhan41 and BINA dhan7. Among the chemicals CaCb showed best as osmopriming agents and it enhanced all germination parameters. BRRI dhan41 showed the best performance. Better root length and shoot length were observed in control. Seeds of BRRI dhan41 when treated with NaCl showed the highest germination percentage (98.67%), germination energy (88%), germination speed (96.83%), but vigor index (10.63) was found highest in BRRI dhan40 when it was treated with KC1. BRRI dhan41 produced the largest root (8.56 cm) when seeds were treated with NaCl. BRRI dhan40 (10.51 cm) and BINA dhan7 (10.53 cm) produced the highest shoot length at controlled condition and CaCl2 treated seed, respectively.   DOI: http://dx.doi.org/10.3329/jbau.v10i1.12013   J. Bangladesh Agril. Univ. 10(1): 15–20, 2012  


2013 ◽  
Vol 3 (3) ◽  
pp. 127-130
Author(s):  
Manoj Kumar Rawat ◽  
Rajendra Prasad Meena

Antimutagenicity of vitamin‐C (ascorbic acid) and L‐cysteine (amino acid) was determined against the mutagenicity of sodium azide (NaN3; pH 6.0) in Trigonella foenum‐graecum L. Toxicity to seed germination and seedling growth were taken as as the parameters. Sodium azide (mutagenic agent) induces inhibitory effects on the seed germination and causes formation of abnormal seedlings significantly with increasing concentrations. Sodium azide mutagenized seeds of T. foenum‐graecum post‐treated with four different aqueous concentrations of vitamin‐C (0.0005M, 0.0010M, 0.0015M and 0.0100M) and L‐cysteine (0.0005M, 0.0010M, 0.0015M and 0.0100M), exhibited antimutagenic activity by way of promotory effects on seed germination as well as on seedling growth as compared to contol. L‐cystein was found to be more potent as antimutagen (antitoxic) than vitamin‐C.


2019 ◽  
Author(s):  
Zhenhua Li ◽  
Xianya Wang ◽  
Yiling Liu ◽  
Min Zhang

Abstract Background: Phytochrome is the most abundant photoreceptor in Arabidopsis thaliana ( Arabidopsis ), which integrates light and temperature signals, and in turn regulates plant development. However, the exact pattern of integrated signals during seed germination remains unknown. In this study, we analyzed the effect of NtPHYB1 genotype in response to ecological environments in Nicotiana tabacum L. Results: The germination frequencies of WT seeds showed at least no significant difference, and were significantly higher than that of NtPHYB1 - GFP and NtPHYB1 -RNAi seeds in some environments or. According to the maximum germination frequency , germination of NtPHYB1 - GFP seeds was mainly inhibited by continuous light exposure, while the germination of NtPHYB1- RNAi seeds was repressed by low temperature and no light (darkness) exposure. At 15ºC, the germinations of all three genotypic seeds were inhibited by the low-temperature, and the germination frequency of NtPHYB1 - GFP seeds was significantly lower than that of WT and NtPHYB1 -RNAi seeds; while light signal had no effect at 15ºC. At 20 and 25ºC, the temperature signal promoted germination, and the signal of light was dispensable. At this condition, the maximum germination frequencies were obtained for NtPHYB1 - GFP and WT seeds. At 30 and 35ºC, the light signal was indispensable to maintain seed germination for all three genotypic seeds. At this condition, NtPHYB1 -RNAi seeds reached the maximum germination frequency. Conclusion: Phytochrome B regulates seed germination by integrating light and temperature signals. The above results elucidate why warm spring and autumn (about 25ºC) are more suitable for sowing compared to cool winters (less than 15ºC) and hot summers (greater than 30ºC).


2018 ◽  
Vol 24 (2) ◽  
Author(s):  
SUPRIYA DIXIT ◽  
R. K. GUPTA

Currently, a real challenge for the workers in the agricultural research field is to stop or reduce the use of expensive agrochemicals/ chemical fertilizers which are hazardous to the environment as well as human health. Present study was aimed to improve the growth and obtain optimum yield of Vigna crop with eco-friendly, non-toxic way and to reduce the use of agrochemical/chemical fertilizer application in agricultural activities. A pot experiment was conducted to study the effect of chemical fertilizer (DAP) and biofertilizer ( Rhizobium strain) separately and in combination on seed germination and seedling growth (at 30 days) based on morphological parameters such as seedling length (cm), fresh weight (g), dry weight (g) and leaf area (cm)2 of Vigna radiata (L.) Wilczek. After one month (30 Days) observations, it was found that seedling length, fresh and dry weights and leaf area were maximum in T4 and minimum in T15, T7 and T8 favored improved seedling length and leaf area whereas T7, T8, and T9 favored improved fresh and dry weights as compared to control.


Author(s):  
Arne J. Aasen ◽  
Sven-Olof Almquist ◽  
Curt R. Enzell

Abstract35: two isomeric 5,6-Epoxy-3-hydroxy-7-megastigmen-9-ones from Nicotiana tabacum L.


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