Phytochrome B regulates seed germination by integrating light and temperature signals in Nicotiana tabacum L.

2019 ◽  
Author(s):  
Zhenhua Li ◽  
Xianya Wang ◽  
Yiling Liu ◽  
Min Zhang

Abstract Background: Phytochrome is the most abundant photoreceptor in Arabidopsis thaliana ( Arabidopsis ), which integrates light and temperature signals, and in turn regulates plant development. However, the exact pattern of integrated signals during seed germination remains unknown. In this study, we analyzed the effect of NtPHYB1 genotype in response to ecological environments in Nicotiana tabacum L. Results: The germination frequencies of WT seeds showed at least no significant difference, and were significantly higher than that of NtPHYB1 - GFP and NtPHYB1 -RNAi seeds in some environments or. According to the maximum germination frequency , germination of NtPHYB1 - GFP seeds was mainly inhibited by continuous light exposure, while the germination of NtPHYB1- RNAi seeds was repressed by low temperature and no light (darkness) exposure. At 15ºC, the germinations of all three genotypic seeds were inhibited by the low-temperature, and the germination frequency of NtPHYB1 - GFP seeds was significantly lower than that of WT and NtPHYB1 -RNAi seeds; while light signal had no effect at 15ºC. At 20 and 25ºC, the temperature signal promoted germination, and the signal of light was dispensable. At this condition, the maximum germination frequencies were obtained for NtPHYB1 - GFP and WT seeds. At 30 and 35ºC, the light signal was indispensable to maintain seed germination for all three genotypic seeds. At this condition, NtPHYB1 -RNAi seeds reached the maximum germination frequency. Conclusion: Phytochrome B regulates seed germination by integrating light and temperature signals. The above results elucidate why warm spring and autumn (about 25ºC) are more suitable for sowing compared to cool winters (less than 15ºC) and hot summers (greater than 30ºC).

Author(s):  
Monika Agacka-Mołdoch ◽  
Mian Abdur Rehman Arif ◽  
Ulrike Lohwasser ◽  
Teresa Doroszewska ◽  
Ramsey S. Lewis ◽  
...  

AbstractGenetic mapping of seed germination traits has been performed with many plant species. In tobacco, however, investigations are rare. In the present study, a bi-parental mapping population consisting of 118 doubled haploid lines and derived from a cross between ‘Beinhart-1000’ and ‘Hicks’ was investigated. Four germination-related traits, total germination (TG), normal germination (NG), time to reach 50% of total germination (T50), and the area under the curve after 200 h of germination (AUC) were considered by examining seeds either untreated or after a moderate controlled deterioration (CD). Quantitative trait loci were found for all traits distributed on 11 out of the 24 linkage groups. It was demonstrated that, as in many other species, germination-related traits are very complex and under polygenic control.


2018 ◽  
Vol 94 (2) ◽  
pp. 220-228 ◽  
Author(s):  
Fabiana de Lima Abrantes ◽  
Alessandra Ferreira Ribas ◽  
Luiz Gonzaga Esteves Vieira ◽  
Nelson Barbosa Machado-Neto ◽  
Ceci Castilho Custódio

1969 ◽  
Vol 11 (1) ◽  
pp. 72-76 ◽  
Author(s):  
Bronius Povilaitis

Seeds of three flue-cured tobacco (Nicotiana tabacum L.) varieties, Hicks Broadleaf, Yellow Gold, and Canadel, were soaked for 1, 2, 3 or 5 hours in a saturated solution of diethyl sulphate, a chemical mutagenic agent, to increase variability of plant characteristics. Diethyl sulphate had a detrimental effect on both extent and rate of germination; each decreased with length of soaking period. Seedlings from treated seed grew more slowly than controls.


HortScience ◽  
1994 ◽  
Vol 29 (8) ◽  
pp. 854-857
Author(s):  
William J. Carpenter ◽  
Eric R. Ostmark ◽  
John A. Cornell

The role of light on impatiens seed germination and radicle emergence was studied. Seeds having a photodormancy require light for only part of the germination period. Germination ≥85% was achieved after 3, 2, or 1 day of irradiance at 1.5, 15, or 75 μmol·s-1·m-2, respectively. Keeping imbibed seeds in darkness for ≥2 days before light exposure caused reduced total germination percentages (G), delayed achieving 50% of the final germination percentage (T50), and increased the days between 10% and 90% germination (T90-T10). Light for 6 hours daily at 1.5, 15, or 150 μmol·s-1·m-2 promoted high G and rapid and uniform germination, but daily 12 to 24 hours of irradiance decreased G and increased T50 and T90-T10. Estimated rates of decline (increase) in G, T50, or T90-T10 with each added day of light (darkness) or increasing daily hours of light were measured by fitting regression equations. Impatiens seed germination was promoted by the initial 1 to 3 days of light, but light inhibited radicle extension in the latter germination stages.


Plants ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1260
Author(s):  
Petra Lovecká ◽  
Anna Macůrková ◽  
Kamil Záruba ◽  
Tomáš Hubáček ◽  
Jakub Siegel ◽  
...  

Tobacco seedlings (Nicotiana tabacum L cv. Wisconsin 38) were treated for 24 h with colloidal solution of silver and gold nanoparticles (AgNPs and AuNPs) of different size or cultivated for 8 weeks on soil polluted with these NPs. DNA damage in leaf and roots nuclei was evaluated by the comet assay. AgNPs of the size 22–25 nm at concentrations higher than 50 mg·L−1 significantly increased the tail moments (TM) values in leaf nuclei compared to the negative control. Ag nanoparticles of smaller size 12–15 nm caused a slight increase in tail moment without significant difference from the negative control. The opposite effect of AgNPs was observed on roots. The increasing tail moment was registered for smaller NPs. Similar results were observed for AuNPs at a concentration of 100 mg·L−1. DNA damaging effects after growing tobacco plants for 8 weeks in soil polluted with AgNPs and AuNPs of different size and concentrations were observed. While lower concentrations of both types of particles had no effect on the integrity of DNA, concentration of 30 mg·kg−1 of AgNPs caused significant DNA damage in leaves of tobacco plants. AuNPs had no effect even at the highest concentration. The content of Ag was determined by ICP–MS in above-ground part of plants (leaves) after 8 weeks of growth in soil with 30 mg·kg−1.AgNPs and was 2.720 ± 0.408 µg·g−1. Long term effect is much less harmful probably due to the plant restoration capability.


2020 ◽  
Vol 10 (3) ◽  
pp. 96
Author(s):  
SAMSURI TIRTOSASTRO ◽  
ABI DWI HASTONO ◽  
. DARMONO

<p>Oven portabel-horisontal (4m x 8m x 4m) untuk pengovenan daun tembakau Virginia menjadi krosok fc (flue-cured) telah direkayasa di Balai Penelitian Tanaman Tembakau dan Serat, Malang, Indonesia. Konstruksi oven terdiri alas komponen dinding oven berisolasi (80cm x 200cm), kerangka dari besi siku dan bcsi U, kolektor surya datar (solar flat- collector) yang dipasang pada atap oven. Sistem pcmanas kompor Bros dengan pemanasan tidak langsung. Pengujian dilakukan pada bulan Agustus sampai dengan Oktober 2002 di Lombok Timur, Nusa Tenggara Barat. Model oven portabel-horisontal ini diharapkan dapat menghasilkan suhu niang oven yang seragam sehingga dapat digunakan untuk mengoven daun tembakau yang persentase masak optimalnya tinggi (90- 95%), hemat bahan bakar, dapat dipasang mendekati areal tanaman sehingga hemat ongkos angkut dan dapat menekan kerusakan pasca panen. Hasil penelitian menunjukkan rata-rata selisih suhu ruang atas dan ruang bawah, serta ruang sebclah kanan dan sebelah kiri, masing-masing 2.53°C dan 2.30°C sedangkan selisih kelembaban udara pada posisi yang sama masing-masing hanya 4.55% dan 3.64%. Kadar gula krosok yang dihasilkan berkisar antara 17.19-19.47% dan nikotin 3.16-3.73%. Konsumsi minyak tanah 1.09 l/kg krosok atau 1.16 l/kg krosok jika tanpa kolektor surya dan hanya 40.92% dibanding oven biasa yang memeriukan 2.39-2.80 l/kg krosok. Kolektor surya datar menyumbang 952 625 kJ setara 28.24 I minyak tanah atau 5.80% dari konsumsi minyak tanah, meskipun alat ini memeriukan 17.16% dari total investasi oven. Panas yang hilang melalui dinding karena konduksi hanya mencapai 3.57%, lebih rendah dibanding kehilangan panas pada dinding bata yang mencapai 12.7% - 16.0%. Hasil grading krosok menghasilkan harga jual rata-rata Rp. 12.275/kg kosok, sedangkan hasil analisis ekonomi menunjukkan rasio BC = 1.29; NPV = Rp. 41 962 590 dan IRR = 51.83% atau masih membei peluang keuntungan dan pengembalian kredit. Hasil perhitungan simulasi jika digunakan oven horisontal sederhana dengan harga Rp. 8 000 000 tiap unit, harga krosok Rp. 10 000, Rp. 12 500, dan Rp. 15 000 tiap kg dan harga minyak tanah Rp. 1 000, Rp. I 250 dan Rp. I 500 tiap liter masih memberi indikasi peluang keuntungan dan pengembalian kredit. Konstruksi oven akan lebih sederhana jika digunakan kerangka kayu dan tanpa kolektor surya.</p><p>Kata kunci : Nicotiana tabacum, L, tembakau, prosesing, oven, portabel- horisontal, kolektor surya, analisis energi, mutu krosok, aspek ekonomi</p><p> </p><p><strong>ABSTRACT </strong></p><p><strong>Engineering of horizontal-potabel curing-barn of Virgi¬ nia tobacco curing</strong><br /><br />Engineeing of the horizontal-portable curing-barn (4m x 8m x 4m) of Virginia tobacco curing lo produce Virginia fc (flue-cured) tobacco conducted in Indonesian Tobacco and Fibers Crops Research Institute, Malang, Indonesia. The curing-bam construction consisted of portable isolation wall, metal frame from L and U iron-bar and flat solar collector which was installed in curing-bam roof. Bros buner and air indirect heating system were used. This curing-bam was tested in East Lombok, West Nusa Tenggara on virgina tobacco harvested in August to October 2002. This horizontal-portable curing-bam was expected to be able to<br /><br />96 <br /><br />produce the homogen temperature and humidity in all space of the curing- bam so that it is suitable for curing the harvesting tobacco leaves which high percentage of mature leaves (90-95%), fuel efficienct and liable to be constructed near tobacco plant area, so that it can decrease the transpotation cost and postharvest damage. The result of the research showed that there were significant difference between above-space and lower-space, let-space and right-space, even it was only 2.53°C and 2.30°C, respectively. The same position for air humidity, 4.55% and 3.64%, respectivelly. Kerosene fuel consumption 1.09 I each kg cured- leaves or 1.16 I each kg cured leaves if without lat solar collector, lower than farmers conventional curing-bam which consumpt 2.39-2.80 I each kg cured-leaves. Flat solar-collector contributed 952 625 Id only or 5.80% of kerosene consumption or equal lo 28.24 I kerosene, even though this equipment needed 17.16% of total curing-bam in vestal ion Heat conduction lost through the portable wall only 3.57%, lower than brickwall conventional curing-bam which reach 12.7-16.0%. Result of the cured-leaves grading gave the average price Rp 12 275,- each kg cured- leaves and economic analysis showed that BC-ratio-1.29, NPV-41 962 590 and IRR=51.83% or still gave the profit chance and ability to pay the capital interest. Result of simulate calculation by simple construction curing-bam, Rp. 8 000 000 price each unit, still gave BC-ratio above one percent. This indicated the profit chance and ability to pay the capital interest. In this simulate calculation three price of cured-leaves and kerosene was used, as followed Rp. 10 000, Rp. 12 500, and Rp. 15 000 each kg cured leaves, and Rp. I 000, Rp. 1 250 and Rp. I 500 each liter of kerosene, respectivelly. The construction of the curing-bam will be simpler if using wood frame and without lat solar collector.</p><p>Key words : Nicotiana tabacum, L., tobacco, processing, oven, horizontal- portable curing-bam, lat solar-collector, energy analysis, cured-leaves grade, economical-aspect</p>


1987 ◽  
Vol 67 (4) ◽  
pp. 1231-1234 ◽  
Author(s):  
MARK J. KING

Exposure of tobacco (Nicotiana tabacum L.) seedlings to low ambient air temperatures during the 3 weeks prior to transplanting resulted in premature flowering. The percent premature flowering observed varied with the location of the experiment, the year, and the total degree units of temperature below 20 °C. A positive relationship between accumulated hourly temperatures below 20 °C and percent premature flowering was observed over four years at two locations.Key words: low temperature, premature flowering


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