A COMPARATIVE STUDY OF THE PRODUCTION OF RNA BYROUND SPOREMUTATIONS INASCOBOLUS IMMERSUS

1974 ◽  
Vol 16 (1) ◽  
pp. 39-48 ◽  
Author(s):  
C. Hamelin ◽  
G. H. Cousineau

Ninety-nine spontaneous round spore mutants from Ascobolus immersus were grouped into two independent series designated 828 and 1034. Series 828 is a complex locus with various alleles. Total RNA characterization of three mutants from series 828 and one from series 1034 was done. Significant quantitative differences in RNA content were found between the mutant strains and the wildtype strain S2. In the case of the particular mutant strains, it seems that the expression on RNA synthesis by heteroallelic strains is identical; while it varies for an independent locus it is still responsible for the same morphological aberration in ascospore size.


ACS Omega ◽  
2021 ◽  
Vol 6 (5) ◽  
pp. 3587-3601
Author(s):  
Hend Abd-Allah ◽  
Maha Nasr ◽  
Omar A. H. Ahmed-Farid ◽  
Salma A. El-Marasy ◽  
Rofanda M. Bakeer ◽  
...  










Genetics ◽  
2002 ◽  
Vol 162 (1) ◽  
pp. 381-394 ◽  
Author(s):  
Craig A Webb ◽  
Todd E Richter ◽  
Nicholas C Collins ◽  
Marie Nicolas ◽  
Harold N Trick ◽  
...  

AbstractIn maize, the Rp3 gene confers resistance to common rust caused by Puccinia sorghi. Flanking marker analysis of rust-susceptible rp3 variants suggested that most of them arose via unequal crossing over, indicating that rp3 is a complex locus like rp1. The PIC13 probe identifies a nucleotide binding site-leucine-rich repeat (NBS-LRR) gene family that maps to the complex. Rp3 variants show losses of PIC13 family members relative to the resistant parents when probed with PIC13, indicating that the Rp3 gene is a member of this family. Gel blots and sequence analysis suggest that at least 9 family members are at the locus in most Rp3-carrying lines and that at least 5 of these are transcribed in the Rp3-A haplotype. The coding regions of 14 family members, isolated from three different Rp3-carrying haplotypes, had DNA sequence identities from 93 to 99%. Partial sequencing of clones of a BAC contig spanning the rp3 locus in the maize inbred line B73 identified five different PIC13 paralogues in a region of ∼140 kb.



1998 ◽  
Vol 18 (7) ◽  
pp. 4337-4346 ◽  
Author(s):  
Vincent Colot ◽  
Vicki Haedens ◽  
Jean-Luc Rossignol

ABSTRACT Upon insertion, transposable elements can disrupt or alter gene function in various ways. Transposons moving through a cut-and-paste mechanism are in addition often mutagenic when excising because repair of the empty site seldom restores the original sequence. The characterization of numerous excision events in many eukaryotes indicates that transposon excision from a given site can generate a high degree of DNA sequence and phenotypic variation. Whether such variation is generated randomly remains largely to be determined. To this end, we have exploited a well-characterized system of genetic instability in the fungus Ascobolus immersus to perform an extensive study of excision events. We show that this system, which produces many phenotypically and genetically distinct derivatives, results from the excision of a novel Ds-like transposon,Ascot-1, from the spore color gene b2. A unique set of 48 molecularly distinct excision products were readily identified from a representative sample of excision derivatives. Products varied in their frequency of occurrence over 4 orders of magnitude, yet most showed small palindromic nucleotide additions. Based on these and other observations, compelling evidence was obtained for intermediate hairpin formation during the excision reaction and for strong biases in the subsequent processing steps at the empty site. Factors likely to be involved in these biases suggest new parallels between the excision reaction performed by transposons of thehAT family and V(D)J recombination. An evaluation of the contribution of small palindromic nucleotide additions produced by transposon excision to the spectrum of spontaneous mutations is also presented.





2012 ◽  
Vol 19 (7) ◽  
pp. 857-864 ◽  
Author(s):  
Gang Song ◽  
Eduardo Mortani Barbosa ◽  
Nicholas Tustison ◽  
Warren B. Gefter ◽  
Maryl Kreider ◽  
...  


Sign in / Sign up

Export Citation Format

Share Document