INSECT CHROMOSOME BANDING: TECHNIQUE FOR G- AND Q-BANDING PATTERN IN THE MOSQUITO AEDES ALBOPICTUS

1975 ◽  
Vol 17 (2) ◽  
pp. 241-244 ◽  
Author(s):  
Gerald E. Steiniger ◽  
Asit B. Mukherjee
Keyword(s):  
Aedes Albopictus ◽  
Banding Pattern ◽  
Chromosome Banding ◽  
Banding Technique ◽  
Modified Technique ◽  
Metaphase Chromosomes ◽  
Somatic Metaphase

A modified technique is described for the production of clear G- and Q-bands of somatic metaphase chromosomes of the mosquito, Aedes albopictus Skuse.

DIFFERENTIAL GIEMSA STAINING IN PLANTS. VI. CENTROMERIC BANDING

1979 ◽  
Vol 21 (3) ◽  
pp. 373-378 ◽  
Author(s):  
W. Gary Filion ◽  
David H. Blakey
Keyword(s):  
Room Temperature ◽  
Chromosome Banding ◽  
Giemsa Staining ◽  
Barium Hydroxide ◽  
Banding Technique ◽  
Giemsa Banding ◽  
Metaphase Chromosomes ◽  
Somatic Metaphase ◽  
C Banding ◽  
Hydrolysis Temperature

Somatic metaphase chromosomes of Tulipa which were subjected to various hydrolyses with several times and temperatures displayed two distinctive types of C-banding when stained using the BSG (Barium hydroxide/Saline/Giemsa) chromosome banding technique. In addition to the two types of Giemsa bands, namely intercalary/terminal and centromeric, a unique transition from the former to the latter type of banding was observed. That is, at the point of transition from intercalary/terminal to centromeric banding, both types were present at one time. The two types of Giemsa banding resulted from different HCl hydrolysis times and temperatures; centromeric bands being observed after either a prolonged hydrolysis at room temperature or an increase in the hydrolysis temperature to 60 °C. These results are discussed in relation to the mechanisms of chromosome banding.

INSECT CHROMOSOME ISOLATION: A METHOD FOR METAPHASE CHROMOSOME HARVEST FROM CULTURED CELLS OF THE MOSQUITO AEDES ALBOPICTUS

1981 ◽  
Vol 23 (3) ◽  
pp. 453-457 ◽  
Author(s):  
Asit B. Mukherjee ◽  
Lorraine DeGiorgio
Keyword(s):  
Cell Line ◽  
Aedes Albopictus ◽  
Metaphase Chromosome ◽  
Cultured Cells ◽  
Modified Technique ◽  
Metaphase Chromosomes ◽  
Chromosome Isolation

A modified technique for bulk isolation of metaphase chromosomes from an in vitro cell line of the mosquito Aedes albopictus (Skuse) is described.

scholarly journals Light and scanning electron microscopy of the same human metaphase chromosomes

1985 ◽  
Vol 77 (1) ◽  
pp. 143-153
Author(s):  
C.J. Harrison ◽  
E.M. Jack ◽  
T.D. Allen ◽  
R. Harris
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Electron Microscope ◽  
Scanning Electron Microscope ◽  
Banding Pattern ◽  
Light Microscope ◽  
Chromosome Banding ◽  
Metaphase Chromosomes ◽  
Scanning Electron

A technique has been developed to examine the same G-banded human metaphase chromosomes, first in the light microscope and then in the scanning electron microscope (SEM). A structural involvement in chromosome banding was confirmed by a positional correlation between the G-positive bands observed in the light microscope and the circumferential grooves between the quaternary coils of the metaphase chromosomes, observed in the SEM. In further support of this the regions between the grooves showed a positional relationship with the G-negative or reverse (R) bands. The examination of slightly extended metaphase chromosomes in the light microscope demonstrated that the G-banding pattern corresponded to that described by the Paris nomenclature for metaphase chromosomes. The arrangement of the circumferential grooves of the same chromosomes, observed in the SEM, was shown to relate to that described by the Paris nomenclature for prometaphase chromosomes. Therefore, using the SEM it is possible to demonstrate the details of prometaphase banding in metaphase chromosomes.

Fluram induces species-dependent C and G bands in mammalian chromosomes, revealing heterogeneous distribution of chromosomal proteins

Genome ◽  
1991 ◽  
Vol 34 (5) ◽  
pp. 772-776 ◽  
Author(s):  
T. Cuéllar ◽  
J. Gosálvez ◽  
P. Del Castillo ◽  
J. C. Stockert
Keyword(s):  
Banding Pattern ◽  
Chromosome Banding ◽  
Chromosome Region ◽  
Centromeric Heterochromatin ◽  
Primary Amines ◽  
Metaphase Chromosomes ◽  
Heterogeneous Distribution ◽  
Chromosomal Proteins ◽  
Differential Reactivity ◽  
Chromosome Regions

Fluram (Fluorescamine; 4-phenylspiro(furan-2(3H),1′-phthalan)-3,3′-dione) is a fluorogenic reagent, which permits the detection of primary amines by forming highly fluorescent pyrrolinone derivatives. This reagent has been used on methanol – acetic acid fixed metaphase chromosomes of mouse and man and proved to be very effective in differentiating chromosome regions in both genomes. Mouse centromeric heterochromatin is highly reactive, showing intense fluorescence in all centromeric regions, whereas human chromosomes show no fluorescence in such regions. In addition, a G-like banding pattern is also obtained in both types of chromosomes. The differential reactivity of each chromosome region showed by this method demonstrates a heterogeneous distribution of chromosome proteins, resulting in a chromosome banding pattern, which is in this case species dependent.Key words: cytogenetics, chromosome banding, mammalian chromosomes, fluorescence microscopy, Fluram.

AnIn VivoGiemsa Chromosome Banding Technique

1975 ◽  
Vol 50 (6) ◽  
pp. 371-374 ◽  
Author(s):  
Robert M. Kitchin ◽  
Eric J. Loudenslager
Keyword(s):  
Chromosome Banding ◽  
Banding Technique

scholarly journals Chromosome banding pattern in a polymorphic population of Sorex araneus from northeastern Finland

Hereditas ◽  
2009 ◽  
Vol 76 (2) ◽  
pp. 305-314 ◽  
Author(s):  
LIISA HALKKA ◽  
O. HALKKA ◽  
U. SKARÉN ◽  
VERONICA SÖDERLUND
Keyword(s):  
Banding Pattern ◽  
Chromosome Banding ◽  
Sorex Araneus

scholarly journals Electron microscopy of gold-labeled human and equine chromosomes.

1989 ◽  
Vol 37 (9) ◽  
pp. 1443-1447 ◽  
Author(s):  
P E Messier ◽  
R Drouin ◽  
C L Richer
Keyword(s):  
Electron Microscopy ◽  
Chromosome Banding ◽  
Protein A ◽  
Gold Complex ◽  
Chromosome Identification ◽  
Chromosomal Dna ◽  
Metaphase Chromosomes ◽  
Sister Chromatids ◽  
Antigen Localization ◽  
Chromatin Reorganization

We present an immunochemical technique for the detection of 5-bromo-2'-deoxyuridine (BrdU) incorporated discontinuously into the chromosomal DNA. A monoclonal anti-BrdU antibody and a protein A-gold complex were used to produce chromosome banding of human and equine chromosomes, specific for electron microscopy (EM). Well-defined bands, symmetry of sister chromatids, concordance between homologues, and band patterns similar to those observed by light microscopy facilitate chromosome identification and karyotyping. From prophase to late metaphase, chromosomes condense and bands appear to fuse. The fusion appears to be owing to chromatin reorganization. Our results underline the value of using immunogold reagents, which are ideal probes for antigen localization on chromosomes.

In situ localization of two repetitive DNA sequences to surface-spread pachytene chromosomes of rye

Genome ◽  
1992 ◽  
Vol 35 (4) ◽  
pp. 551-559 ◽  
Author(s):  
S. M. Albini ◽  
T. Schwarzacher
Keyword(s):  
In Situ Hybridization ◽  
Synaptonemal Complex ◽  
Dna Sequences ◽  
Meiotic Prophase ◽  
Hybridization Signal ◽  
Metaphase Chromosomes ◽  
Somatic Metaphase ◽  
Rdna Signal ◽  
Surface Spread

Surface-spread pollen mother cells at meiotic prophase from Secale cereale (rye) were used for fluorescent DNA:DNA in situ localization of two tandemly repeated DNA sequences: pTa71, a wheat rDNA clone, and pSc119.2, a cloned 120-bp repeat from rye heterochromatin. The fluorescent hybridization signal, consisting of many yellow-green dots, was closely associated with the bivalent axes, corresponding to the synaptonemal complex, and located in the surrounding chromatin. The rDNA signal was associated with one bivalent, the smallest of the seven, at a distance about 13% of the bivalent length from the telomere. This corresponded to the position of the nucleolar organizing region of silver-stained synaptonemal complexes analyzed under the electron microscope and published data for somatic metaphase chromosomes. The relative length of the axis covered with the rDNA signal is less than expected from somatic metaphases, but it corresponds more closely to the proportion of the sequences in the genome. The hybridization signal with the 120-bp repeat was located mainly at the telomeric regions of several bivalents that showed thickenings of the axis after DAPI staining, probably corresponding to somatic C-bands. These major and some minor intercalary sites agree with the distribution of the 120-bp repeat in somatic metaphase. Fluorescent in situ hybridization to plant surface-spread pachytene chromosomes, which can be obtained in large numbers, has great potential for studying meiotic prophase, high-resolution mapping of DNA sequences, and investigating the relationship of DNA sequences to the synaptonemal complex.Key words: in situ hybridization, cereals, pachytene, meiosis, synaptonemal complex, physical mapping.

A new chromosome banding technique, spectral color banding (SCAN), for full characterization of chromosomal abnormalities

2003 ◽  
Vol 37 (4) ◽  
pp. 412-416 ◽  
Author(s):  
Naoki Kakazu ◽  
Irit Bar-Am ◽  
Satoshi Hada ◽  
Hiroatsu Ago ◽  
Tatsuo Abe
Keyword(s):  
Chromosome Banding ◽  
Chromosomal Abnormalities ◽  
Banding Technique ◽  
Full Characterization
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