NUCLEAR SUPPRESSORS OF THE [POKY] CYTOPLASMIC MUTANT IN NEUROSPORA CRASSA. I. GENETICS AND RESPIRATORY PROPERTIES
Six nuclear suppressors of the [poky] cytoplasmic mutant (sup-1, sup-3, sup-4, sup-5, sup-10, sup-14) have been obtained in Neurospora crassa. The sup genes suppress the slow growth phenotype of [poky], and alleviate, at least partially, the deficiency of cyanide-sensitive respiratory activity in the mycelium of this cytoplasmic mutant. The six suppressors are nonallelic, suppress the phenotypic effects of [stp-Bl] in addition to [poky], but have no effect on the phenotypic expression of the [mi-3] cytoplasmic mutant. On the basis of experimentally established molecular defects in [poky] and on the basis of hypothetical considerations, it is proposed that the sup mutations affect the structure and properties of mitochondrial ribosomal proteins.