A species-specific satellite DNA from the entomopathogenic nematode <i>Heterorhabditis indicus</i>

An AluI satellite DNA family has been cloned from the entomopathogenic nematode Heterorhabditis indicus. This repeated sequence appears to be an unusually abundant satellite DNA, since it constitutes about 45% of the H. indicus genome. The consensus sequence is 174 nucleotides long and has an A + T content of 56%, with the presence of direct and inverted repeat clusters. DNA sequence data reveal that monomers are quite homogeneous. Such homogeneity suggests that some mechanism is acting to maintain the homogeneity of this satellite DNA, despite its abundance, or that this repeated sequence could have appeared recently in the genome of H. indicus. Hybridization analysis of genomic DNAs from different Heterorhabditis species shows that this satellite DNA sequence is specific to the H. indicus genome. Considering the species specificity and the high copy number of this AluI satellite DNA sequence, it could provide a rapid and powerful tool for identifying H. indicus strains.Key words: AluI repeated DNA, tandem repeats, species-specific sequence, nucleotide sequence analysis.

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Characterization of a species-specific satellite DNA from the entomopathogenic nematode Steinernema carpocapsae

Molecular and Biochemical Parasitology ◽

10.1016/0166-6851(94)00197-u ◽

1995 ◽

Vol 69 (1) ◽

pp. 93-100 ◽

Cited By ~ 6

Author(s):

Eric Grenier ◽

Christian Laumond ◽

Pierre Abad

Keyword(s):

Satellite Dna ◽

Entomopathogenic Nematode ◽

Steinernema Carpocapsae ◽

Species Specific

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Lack of satellite DNA species-specific homogenization and relationship to chromosomal rearrangements in monitor lizards (Varanidae, Squamata)

BMC Evolutionary Biology ◽

10.1186/s12862-017-1044-6 ◽

2017 ◽

Vol 17 (1) ◽

Cited By ~ 7

Author(s):

Ornjira Prakhongcheep ◽

Watcharaporn Thapana ◽

Aorarat Suntronpong ◽

Worapong Singchat ◽

Khampee Pattanatanang ◽

...

Keyword(s):

Satellite Dna ◽

Chromosomal Rearrangements ◽

Monitor Lizards ◽

Species Specific

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Short communication: Satellite DNA-based species-specific identification of single individuals of the pinewood nematode Bursaphelenchus xylophilus (Nematoda: Aphelenchoididae)

European Journal of Plant Pathology ◽

10.1007/s10658-004-0580-2 ◽

2005 ◽

Vol 112 (2) ◽

pp. 191-193 ◽

Cited By ~ 39

Author(s):

Chantal Castagnone ◽

Pierre Abad ◽

Philippe Castagnone-Sereno

Keyword(s):

Satellite Dna ◽

Short Communication ◽

Bursaphelenchus Xylophilus ◽

Pinewood Nematode ◽

Specific Identification ◽

Communication Satellite ◽

Species Specific

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Entomopathogenic nematodes increase predation success by inducing cadaver volatiles that attract healthy herbivores

eLife ◽

10.7554/elife.46668 ◽

2019 ◽

Vol 8 ◽

Cited By ~ 7

Author(s):

Xi Zhang ◽

Ricardo AR Machado ◽

Cong Van Doan ◽

Carla CM Arce ◽

Lingfei Hu ◽

...

Keyword(s):

Reproductive Success ◽

Natural Enemies ◽

Entomopathogenic Nematodes ◽

Entomopathogenic Nematode ◽

Butylated Hydroxytoluene ◽

Diabrotica Virgifera ◽

Major Pest ◽

Herbivore Species ◽

Species Specific ◽

Volatile Cues

Herbivore natural enemies protect plants by regulating herbivore populations. Whether they can alter the behavior of their prey to increase predation success is unknown. We investigate if and how infection by the entomopathogenic nematode Heterorhabditis bacteriophora changes the behavior of healthy larvae of the western corn rootworm (Diabrotica virgifera), a major pest of maize. We found that nematode-infected rootworm cadavers are attractive to rootworm larvae, and that this behavior increases nematode reproductive success. Nematode-infected rootworms release distinct volatile bouquets, including the unusual volatile butylated hydroxytoluene (BHT). BHT alone attracts rootworms, and increases nematode reproductive success. A screen of different nematode and herbivore species shows that attraction of healthy hosts to nematode-infected cadavers is widespread and likely involves species-specific volatile cues. This study reveals a new facet of the biology of herbivore natural enemies that boosts their predation success by increasing the probability of host encounters.

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Comparative analysis of 1.688 satellite DNA on D. melanogaster species subgroup provides new insights of its pervasive presence throughout both chromatin domains and reveals a recent horizontal transfer event.

10.1101/277129 ◽

2018 ◽

Author(s):

Leonardo Gomes De Lima ◽

Gustavo C. S. Kuhn

Keyword(s):

Phylogenetic Analysis ◽

Comparative Analysis ◽

X Chromosome ◽

Satellite Dna ◽

Horizontal Transfer ◽

Tandem Repeats ◽

Specific Pattern ◽

Chromosome X ◽

Transfer Event ◽

Species Specific

The 1.688 satellite DNA is present in the genome of Drosophila species from the melanogaster subgroup and has never been detected in species outside this subgroup. We investigated the presence and evolution of the 1.688 satDNA in all Drosophila genomes sequenced so far. Blast searches showed that 1.688 repeats are virtually confined to species from the melanogaster subgroup. Phylogenetic analysis of ~6,500 repeats extracted from D. melanogaster , D. simulans , D. sechellia , D. yakuba and D. erecta revealed the presence of 1.688 family on heterochromatin and euchromatin of all five species. Heterochromatic copies revealed a concerted mode of evolution and a species-specific pattern. Oppositely, euchromatic copies lack species-specific or array-specific pattern. Euchromatic arrays also showed a high number of insertions on 5Kb upstream/downstream of genes and in intronic regions. Unexpectedly, we found an array with at least three full 1.688 tandem repeats in the genome of D. willistoni . These repeats were highly similar to the ones present in the chromosome X of D. melanogaster , although both species have diverged from each other more than 35Mya, suggesting that 1.688 repeats from the X chromosome of D. melanogaster moved to D. willistoni by a recent horizontal transfer event.

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The influence of organic matter content and media compaction on the dispersal of entomopathogenic nematodes with different foraging strategies

Parasitology ◽

10.1017/s0031182017001317 ◽

2017 ◽

Vol 144 (14) ◽

pp. 1956-1963 ◽

Cited By ~ 4

Author(s):

APOSTOLOS KAPRANAS ◽

ABIGAIL M. D. MAHER ◽

CHRISTINE T. GRIFFIN

Keyword(s):

Organic Matter ◽

Entomopathogenic Nematode ◽

Organic Matter Content ◽

Matter Content ◽

Steinernema Feltiae ◽

Foraging Strategies ◽

Infective Juvenile ◽

Soil Parameters ◽

Biological Pest Control ◽

Species Specific

SUMMARYIn laboratory experiments, we investigated how media with varying ratio of peat:sand and two levels of compaction influence dispersal success of entomopathogenic nematode (EPN) species with different foraging strategies: Steinernema carpocapsae (ambusher), Heterorhabditis downesi (cruiser) and Steinernema feltiae (intermediate). Success was measured by the numbers of nematodes moving through a 4 cm column and invading a wax moth larva. We found that both compaction and increasing peat content generally decreased EPN infective juvenile (IJ) success for all three species. Of the three species, H. downesi was the least affected by peat content, and S. carpocapsae was the most adversely influenced by compaction. In addition, sex ratios of the invading IJs of the two Steinernema species were differentially influenced by peat content, and in the case of S. feltiae, sex ratio was also affected by compaction. This indicates that dispersal of male and female IJs is differentially affected by soil parameters and that this differentiation is species-specific. In conclusion, our study shows that organic matter: sand ratio and soil compaction have a marked influence on EPN foraging behaviour with implications for harnessing them as biological pest control agents.

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