EFFECT OF PLATING MEDIUM AND INCUBATION TEMPERATURE ON GROWTH OF FUNGI IN SOIL-DILUTION PLATES

1963 ◽  
Vol 9 (6) ◽  
pp. 741-751 ◽  
Author(s):  
Donald D. Kaufman ◽  
Lansing E. Williams ◽  
Charles B. Sumner
Keyword(s):  
Sugar Beet ◽  
Incubation Temperature ◽  
Soil Extract ◽  
Plate Method ◽  
Dilution Plate ◽  
Extract Agar ◽  
Dilution Plate Method ◽  
Incubation Temperatures ◽  
Soil Dilution ◽  
Soil Extract Agar

The effects of 20, 25, or 30 °C incubation temperatures for dilution-plates and of five plating media (Ohio Agricultural Experiment Station Medium (OAES), Liftman"s crystal-violet agar, dextrose-peptone agar, soil-extract agar, and glucose-nitrate soil-extract agar) on total numbers and types of fungi isolated from two soils (sugar beet or corn cropped) by a soil-dilution plate method were determined. The data revealed that significant differences existed among the three variables as well as their interactions. Although numbers of colonies of fungi isolated were not affected significantly, the types of fungi (number of individual genera) isolated were significantly greater in sugar beet than in corn cropped soils. In nearly all instances, the greatest total number and types of fungi were isolated at temperatures of 20 or 25 °C. Although each medium appeared to favor one or more groups of fungi, the overall frequency and distribution of fungi was essentially the same on all media. On the basis of its transparency, total number and types of fungi isolated, the elimination of bacteria and actinomycetes, and its restriction of rapidly growing fungi, the OAES medium was deemed the most suitable for use in the soil-dilution plate method.

Isolation of Potential Alkaliphilic Cellulase-Producer Strains from Limestone Area of Perlis

2015 ◽  
Vol 754-755 ◽  
pp. 1054-1058
Author(s):  
Nor’izzah Zainuddin ◽  
Khadijah Hanim Abd Rahman ◽  
Abdul Razak Shaari ◽  
Siti Jamilah Hanim Mohd Yusof
Keyword(s):  
Selective Medium ◽  
Plate Method ◽  
Dilution Plate ◽  
Cellulolytic Fungi ◽  
The Future ◽  
Alkaline Cellulase ◽  
Dilution Plate Method ◽  
Soil Dilution ◽  
Positive Results ◽  
Producer Strains

Alkaliphilic cellulases-producer fungi were isolated from soil of limestone areas in Perlis. The potential strain was isolated by soil dilution plate method on enriched selective medium using CMC as substrate at different pH levels. Eleven isolates of different morphological colonies were screened using the hydrolysis capacity test by Gram’s iodine. Out of eleven colonies, five showed positive results as the hydrolysis zone formed. BK1 showed the highest hydrolysis capacity among all sample strains for every pH levels. Eventually, four strains were selected to be further explored as cellulolytic fungi for the production of alkaline cellulase in the future.

scholarly journals Microfungi in the soil beneath common oak and their effect on Armillaria occurrence

2014 ◽  
Vol 38 (1-2) ◽  
pp. 149-160
Author(s):  
Hanna Kwaśna
Keyword(s):  
Sporothrix Schenckii ◽  
Plate Method ◽  
Dematiaceous Hyphomycetes ◽  
Dilution Plate ◽  
Dilution Plate Method ◽  
Common Oak ◽  
Soil Dilution

Microfungal assemblages in a soil beneath 30- and 50·year-old oaks and their 2-year-old stumps were studied using the soil dilution plate method. A total of 98 culturable microfungi were isolated. Compared to the living oaks before felling and the control living oaks, the density of <i>Mortierella macrocystis, Penicillium jonczewskii, Pseudogymnoascus roseus Sporothrix schenckii, Tolypoccladiumum inflatum</i> and <i>Umbelopsis vinacea</i> sigificantly inacased in the soil beneath slumps in the 32- and 52-year-old stands. Density of <i>Aspergillus kanagawaensis, Monodictys lepraria, P. daleae</i> and sterile dematiaceous hyphomycetes increased significantly in the 32-year-old stand and <i>Chrysosporium merdarium</i> in the 52·year-old stand. These fungi are known 'stimulants' of <i>Armillaria</i> rhizomorph formation. It is suggested that the increase in density of <i>Armillaria</i> rhizomorph 'stimulants' in a soil beneath oak stumps may increase the possibility of colonization of stumps by <i>Armillaria</i>.

PLATE COUNTS OF BACTERIA AND FUNGI IN A SALINE SOIL

1959 ◽  
Vol 5 (5) ◽  
pp. 431-439 ◽  
Author(s):  
Norman James
Keyword(s):  
Saline Soil ◽  
Potassium Phosphate ◽  
Soil Extract ◽  
Red River ◽  
Replica Plating ◽  
Extract Agar ◽  
Plate Counts ◽  
Agar Media ◽  
Bacteria And Fungi ◽  
Soil Extract Agar

Numbers of bacteria and of fungi in a saline soil were about one-fifth of numbers in a Red River clay soil. Bacterial counts on two soil-extract agar media, one prepared from the saline soil and the other from Red River soil, were the same, and the populations on the two were the same as shown by the replica plating technique. They were larger than counts on sodium albuminate agar or on asparagin–mannitol agar. Likewise, fungal counts on either soil-extract agar, with 0.02% dipotassmm phosphate, 0.1% glucose, and 0.1% peptone, were higher than counts on Waksman's acidified medium, or on Martin's medium, or on Smith and Dawson's medium. Interestingly, fungal counts on a medium with the same three chemicals as the soil-extract media but with the soil extract replaced by water were as high as those on the soil-extract media. Different levels of potassium phosphate were tested in each of the above media. In each medium for bacteria, and in each for fungi, counts varied inversely as the amount of potassium phosphate. The same held true when sodium phosphate was used instead of potassium phosphate in each medium.

Accuracy and precision of population estimates of Verticillium dahliae on growth media in quantitative soil assays

2003 ◽  
Vol 81 (4) ◽  
pp. 294-306 ◽  
Author(s):  
S I Mpofu ◽  
R Hall
Keyword(s):  
Population Density ◽  
Verticillium Dahliae ◽  
Soil Extract ◽  
Population Estimates ◽  
Dilution Method ◽  
Growth Media ◽  
Infested Soil ◽  
Extract Agar ◽  
Accuracy And Precision ◽  
Soil Extract Agar

Verticillium dahliae Kleb. is a serious pathogen of many plant species. Growth media used to measure population density of V. dahliae in soil were evaluated for high recovery of the pathogen, as well as accuracy and precision of population estimates from naturally and artificially infested sandy loam soil using the soil dilution method. Recovery of V. dahliae from naturally infested field soil was highest on soil pectate Tergitol agar (SPT), soil extract agar + sodium polypectate (SEAP), modified pectate agar (MPA), potato dextrose agar + streptomycin sulphate (PDAS), and Talboys' prune lactose agar (TPA); however, PDAS and TPA were overgrown with contaminating fungi, making enumeration difficult. Use of SPT medium resulted in the most precise population estimate with a standard error (SE) of 12.6% of the mean followed by use of pectate agar (PA) (SE = 14.8%) and SEAP (SE = 19.1%). Ethanol, biotin, and Dox salts enhanced recovery of V. dahliae from naturally infested soil, but combining Dox salts with ethanol and biotin significantly reduced population density. Soil extract had no significant effects on population density. Accuracy of V. dahliae population estimates from sterile artificially inoculated soil was highest on modified soil extract agar (MSEA) (64%) followed by SPT (58%). However, accuracy of V. dahliae population estimates from nonsterile artificially inoculated soil was highest on SPT (36%). Soil extract is not an essential ingredient and biotin may increase recovery of V. dahliae from soil.Key words: Verticillium dahliae, verticillium wilt, population density, recovery, accuracy, precision.

scholarly journals Increased culturability of soil bacteria from Marcellus shale temperate forest in Pennsylvania

2013 ◽  
Vol 2 (1) ◽  
pp. 36-42 ◽  
Author(s):  
Evan Mitchell Lutton ◽  
Rosa Schellevis ◽  
Anupama Shanmuganathan
Keyword(s):  
16S Rdna ◽  
Soil Bacteria ◽  
Temperate Forest ◽  
Culture Medium ◽  
Culture Media ◽  
Marcellus Shale ◽  
Soil Extract ◽  
Extract Agar ◽  
Unique Otus ◽  
Soil Extract Agar

Soil bacteria comprise a largely untapped resource with only 1-10% of bacterial species predicted to live in soil being culturable in the laboratory. Establishing culture-dependent protocols that identify unique operational taxonomic units (OTUs) is an important research topic in soil bacterial ecology. The culturability of soil bacteria may be improved by employing different culture media due to inherent preferences of growth substrate utilization. Soil-extract agar, R-2A agar, and 1% nutrient agar were used in this study to isolate bacteria obtained from soil samples collected in winter months to increase the understanding of bacterial diversity in Abernathy Field Station, a Marcellus shale temperate forest in Washington, Pennsylvania. Changes in bacterial diversity can be used to assess the early impact of anthropological factors, such as hydraulic fracturing in the Marcellus shale region, which may lead to severe environmental problems. For the purpose of long term ecological monitoring, data obtained from this year’s sample collection were analyzed in conjunction with previous years’ assessments. Bacterial isolates were analyzed taxonomically and phylogenetically. Unique OTUs were identified through comparative analysis of 16S rDNA. The Shannon-Weaver and Simpson’s diversity indices ranked isolates on soil-extract agar highest for species richness, and rarefaction analysis suggests that sampling saturation of OTUs identified on soil-extract agar has not yet been reached. Each culture medium studied supported isolates of four common phyla: Actinobacteria, Bacteroidetes, Firmicutes, and Proteobacteria. Soil-extract agar supported the greatest proportion of pigmented colonies including a Cyanobacterium which exhibited intra-16S rDNA polymorphism. Each culture medium supported the growth of unique OTUs and genera with Bacillus, Flavobacterium, Pseudomonas, Rhizobium, and Streptomyces found on each. This study suggests that utilizing different culture media can increase the culturability of soil bacteria.

scholarly journals Caracterização de isolados de Rhizoctonia associados à queima foliar em Roraima.

2012 ◽  
Vol 6 (2) ◽  
pp. 158 ◽  
Author(s):  
Dayane Rodrigues Youssef ◽  
Giovanni Ribeiro de Souza ◽  
Kátia De Lima Nechet ◽  
Bernardo De Almeida Halfeld-Vieira
Keyword(s):  
Glycine Max ◽  
Rhizoctonia Solani ◽  
Vigna Unguiculata ◽  
Lactuca Sativa ◽  
Hevea Brasiliensis ◽  
Cajanus Cajan ◽  
Citrullus Lanatus ◽  
Soil Extract ◽  
Extract Agar ◽  
Soil Extract Agar

O objetivo desse trabalho foi caracterizar isolados do fungo Rhizoctonia associados à queima foliar, obtidos de hospedeiros de importância econômica no estado de Roraima. Os isolados foram obtidos de plantas de feijão-caupi (Vigna unguiculata), soja (Glycine max), seringueira (Hevea brasiliensis), melancia (Citrullus lanatus), alface (Lactuca sativa) e feijão-guandu (Cajanus cajan). Os parâmetros utilizados foram números de núcleos, grupo de anastomose e as características culturais da colônia, taxa de crescimento micelial e a formação de escleródios nos meios de cultura: batata dextrose agar (BDA), BDA+asparagina, BDA+extrato de levedura, Czapek Agar, maltose-peptona-agar, soil extract agar, sacarose-yeast-asparagina e V-8. Todos os 10 isolados estudados foram caracterizados como multinucleados e pertencentes à espécie Rhizoctonia solani. Três isolados de feijão-caupi, um de soja e o isolado de melancia foram identificados como AGI-1A e um isolado de feijãocaupi, um de soja e o isolado de feijão-guandu como AGI-1B. O isolado de seringueira não foi identificado como nenhum dos padrões de anastomose utilizado. Para a maioria dos isolados as maiores taxas de crescimento micelial foram obtidas no meio de cultura Soil Extract Agar. Dois tipos de escleródios, característicos do grupo AGI, foram observados: formação de 2-20 tufos.placa-1 coloração variável, 1-2 mm e formação de 38-611 microescleródios placa-1, de coloração marrom, medindo 100 μm. A produção e o tipo de escleródio variaram com o isolado e o meio de cultura utilizado.

SOIL-EXTRACT AGAR FOR CANDIDA ALBICANS

1954 ◽  
Vol 69 (6) ◽  
pp. 735 ◽  
Author(s):  
ALEXANDER BAKERSPIGEL
Keyword(s):  
Candida Albicans ◽  
Soil Extract ◽  
Extract Agar ◽  
Soil Extract Agar

scholarly journals Evaluation of a New Most-Probable-Number (MPN) Dilution Plate Method for the Enumeration of Escherichia coli in Water Samples

2009 ◽  
Vol 14 (3) ◽  
pp. 123-126 ◽  
Author(s):  
HIDEMASA KODAKA ◽  
MIKAKO SAITO ◽  
HIDEAKI MATSUOKA
Keyword(s):  
Escherichia Coli ◽  
Water Samples ◽  
Most Probable Number ◽  
Plate Method ◽  
Probable Number ◽  
Dilution Plate ◽  
Dilution Plate Method

Fruiting of Lyophyllum tylicolor in plate culture on Soytoneglucose agar and urea-treated soil extract agar

Mycoscience ◽  
1994 ◽  
Vol 35 (2) ◽  
pp. 187-189 ◽  
Author(s):  
Takashi Yamanaka
Keyword(s):  
Soil Extract ◽  
Treated Soil ◽  
Extract Agar ◽  
Soil Extract Agar
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