BACTERIAL CELL MEMBRANES: II. POSSIBLE STRUCTURE OF THE BASAL MEMBRANE CONTINUUM OF MICROCOCCUS LYSODEIKTICUS

1967 ◽  
Vol 13 (11) ◽  
pp. 1499-1507 ◽  
Author(s):  
E. A. Grula ◽  
Thomas F. Butler ◽  
Robert D. King ◽  
Gerald L. Smith
Keyword(s):  
Amino Acids ◽  
Membrane Protein ◽  
Layered Structure ◽  
Sodium Lauryl Sulfate ◽  
Basal Membrane ◽  
Lauryl Sulfate ◽  
Protein Membrane ◽  
Micrococcus Lysodeikticus ◽  
Hydrophobic Amino Acids ◽  
Ethyl Acetate Extracts

Treatment of isolated membranes from M. lysodeikitcus with acetone, ethanol, or methanol, but not ethyl acetate, extracts phospholipids and carotenoids. These extracted membranes are not disaggregated by such treatments and retain a three-layered appearance. The extracted membranes can be completely disaggregated by sodium lauryl sulfate, yielding subunits which are primarily protein (stripped subunits). These stripped subunits reaggregate into membranous sheets that also possess a three-layered structure. Conditions for reaggregation of stripped subunits are the same as for unstripped subunits. Extracted phospholipids and carotenoids from M. lysodeikitcus or phospholipids from Erwinia sp. are incorporated into the membranous sheets formed using the stripped subunits from M. lysodeikticus. The composition of the basal membrane continuum from M. lysodeikticus consists primarily of protein; most phospholipids and carotenoids appear to be present as superstructures on the top and bottom of the protein. Membrane protein is basic and does not appear to contain any cysteine. It is speculated that hydrophobic amino acids may be positioned in clusters on the facing sides of the molecules.

BACTERIAL CELL MEMBRANES: I. REAGGREGATION OF MEMBRANE SUBUNITS FROM MICROCOCCUS LYSODEIKTICUS

1967 ◽  
Vol 13 (11) ◽  
pp. 1471-1479 ◽  
Author(s):  
Thomas F. Butler ◽  
Gerald L. Smith ◽  
E. A. Grula
Keyword(s):  
Ionic Strength ◽  
Divalent Cation ◽  
Sodium Lauryl Sulfate ◽  
Small Subunit ◽  
Lauryl Sulfate ◽  
Reducing Conditions ◽  
Micrococcus Lysodeikticus ◽  
Monovalent Salt ◽  
Low Ionic Strength ◽  
Bonding Mode

The membranes from M. lysodeikticus can readily be disaggregated into a very small subunit form with sodium lauryl sulfate. Reaggregation of these subunits into membranous sheets occurs during dialysis in a menstruum of relatively low ionic strength, in the presence of a divalent cation at near neutral pH. Reducing conditions and a monovalent salt need not be present during dialysis. Effect of several compounds on membranes and subunit reaggregation was studied. Reaggregated membrane sheets are very similar chemically and morphologically to whole membranes; however, a difference was noted. The major bonding mode in membranes appears to involve hydrophobic groups.

ASSESSMENT OF THE TOXICITY AND HAZARD OF SODIUM LAURYL SULFATE AT DIFFERENT EXPOSURE ROUTES

2020 ◽  
pp. 56-59
Author(s):  
M. V. Bidevkina ◽  
M. I. Golubeva ◽  
A. V. Limantsev ◽  
I. N. Razumnaya ◽  
T. N. Potapova ◽  
...  
Keyword(s):  
Sodium Lauryl Sulfate ◽  
Exposure Level ◽  
Hazardous Substance ◽  
Lauryl Sulfate ◽  
Upper Respiratory Tract ◽  
Mucous Membranes ◽  
Chloroprene Rubber ◽  
Upper Respiratory ◽  
Exposure Routes ◽  
Number Of Cells

Sodium lauryl sulfate is the most common surfactant used in the production of detergents, chloroprene rubber, plastics, artificial furs and in pharmaceutical industry. Sodium lauryl sulfate is a moderately hazardous substance when introduced into the stomach (DL50 for white mice and rats is in the range of 2086-2700 mg/kg), has a pronounced local irritant effect on the skin and mucous membranes of the eyes, has a skin-resorptive, sensitizing and pronounced cumulative effects. The threshold for acute inhalation action is set at 15,3 mg/m3 for changes in the function of the nervous system and irritating effects on the mucous membranes of the upper respiratory tract (an increase in the total number of cells in the nasal flushes).Recommended for approval tentative safe exposure level of sodium lauryl sulfate in the air of the working area is 0.2+ mg/m3 (aerosol).

13C nuclear magnetic resonance study of cyclodipeptides containing 13C enriched hydrophobic amino acids

1980 ◽  
Vol 45 (2) ◽  
pp. 482-490 ◽  
Author(s):  
Jaroslav Vičar ◽  
François Piriou ◽  
Pierre Fromageot ◽  
Karel Bláha ◽  
Serge Fermandjian
Keyword(s):  
Amino Acids ◽  
Nuclear Magnetic Resonance ◽  
Nuclear Magnetic Resonance Study ◽  
Coupling Constants ◽  
Side Chain ◽  
Amino Acid Residues ◽  
Magnetic Resonance Study ◽  
Side Chain Conformation ◽  
13C Nuclear Magnetic Resonance ◽  
Hydrophobic Amino Acids

The diastereoisomeric pairs of cyclodipeptides cis- and trans-cyclo(Ala-Ala), cyclo(Ala-Phe), cyclo(Val-Val) and cyclo(Leu-Leu) containing 85% 13C enriched amino-acid residues were synthesized and their 13C-13C coupling constants were measured. The combination of 13C-13C and 1H-1H coupling constants enabled to estimate unequivocally the side chain conformation of the valine and leucine residues.

scholarly journals Functional consequences of alterations to hydrophobic amino acids located in the M4 transmembrane sector of the Ca(2+)-ATPase of sarcoplasmic reticulum.

1993 ◽  
Vol 268 (24) ◽  
pp. 18359-18364 ◽  
Author(s):  
D.M. Clarke ◽  
T.W. Loo ◽  
W.J. Rice ◽  
J.P. Andersen ◽  
B. Vilsen ◽  
...  
Keyword(s):  
Amino Acids ◽  
Sarcoplasmic Reticulum ◽  
Functional Consequences ◽  
Hydrophobic Amino Acids

scholarly journals Preparation of alkali-resistant PVDF membranes via immobilization of sodium lauryl sulfate (SDS) on surface

2021 ◽  
Vol 11 (3) ◽  
Author(s):  
Gongpu Wen ◽  
Kun Chen ◽  
Yanhong Zhang ◽  
Yue Zhou ◽  
Jun Pan ◽  
...  
Keyword(s):  
Sodium Lauryl Sulfate ◽  
Alkali Treatment ◽  
Membrane Surface ◽  
Alkali Resistance ◽  
Lauryl Sulfate ◽  
Uv Curable ◽  
Pvdf Membrane ◽  
Glycol Diacrylate ◽  
Polyester Acrylate ◽  
Uv Curable Resin

AbstractA novel strategy was proposed to fabricate alkali-resistant PVDF membrane via sodium lauryl sulfate (SDS) attached to the surface of membrane and immobilized by UV-curable polyester acrylate and tri(propylene glycol) diacrylate (TPGDA). The attached anionic surfactant, SDS, on the membrane surface can resist the alkali corrosion by NaOH, and the curing of the resin can immobilize the SDS on the membrane firmly. Due to the unique alkali resistance of SDS and resin formed, the UV-curable resin-modified PVDF membrane showed greatly enhanced alkali-resistant ability. Characterization of SEM and FTIR showed that polyester acrylate and TPGDA were cured successfully under the action of 1-hydroxycyclohexyl phenyl ketone (184) and ultraviolet light. Whiteness, differential scanning calorimeter and X-ray photoelectron spectrometer characterization showed that the modified PVDF membrane had a lower degree of dehydrofluorination than the pristine PVDF membrane after alkali treatment. Results of the detailed alkali-resistant analysis indicated that the F/C ratio of the UV-curable resin-modified PVDF membrane decreased by 2.6% after alkali treatment compared to pristine PVDF membrane decreased by 19.28%. The alkali-resistant performance was mainly attributed to the immobilized SDS. This study provided a facile and scalable method for designing alkali-resistant PVDF membrane, which shows a promising potential in the treatment of alkaline wastewater and alkaline-cleaning PVDF membrane.

Sodium Lauryl Sulfate in Egg Whites

1967 ◽  
Vol 50 (4) ◽  
pp. 847-849
Author(s):  
John Wiskerchen
Keyword(s):  
Chloride Solution ◽  
Sodium Lauryl Sulfate ◽  
Collaborative Study ◽  
Egg White ◽  
Chloride Complex ◽  
Bromphenol Blue ◽  
Lauryl Sulfate ◽  
Benzethonium Chloride ◽  
Alkyl Sulfates

Abstract A method is given for the quantitative determination of sodium lauryl sulfate in liquid, frozen, powdered, or flake-dried egg white. The egg white is dissolved in water and the protein is precipitated with ethanol and filtered off. The filtrate is evaporated, the residue is dissolved in water, and the pH is adjusted to 5.0. Total alkyl sulfates are titrated with standard benzethonium chloride solution in the presence of chloroform with bromphenol blue indicator. Results are calculated as sodium lauryl sulfate. The formation of the bromphenol bluebenzethonium chloride complex, when excess benzethonium chloride is present, is taken as the end point. The blue-green complex is soluble in the chloroform. Overall recoveries of sodium lauryl sulfate from egg whites ranged from 94 to 100%. Collaborative study of the method is recommended.

The distribution of alkyl paraben in aqueous sodium lauryl sulfate micellar solutions

1978 ◽  
Vol 66 (1) ◽  
pp. 26-32 ◽  
Author(s):  
Ayako Goto ◽  
Fumio Endo
Keyword(s):  
Sodium Lauryl Sulfate ◽  
Lauryl Sulfate ◽  
Micellar Solutions ◽  
Aqueous Sodium

Oral Fluoride Levels 1 h after Use of a Sodium Fluoride Rinse: Effect of Sodium Lauryl Sulfate

2015 ◽  
Vol 49 (3) ◽  
pp. 291-296 ◽  
Author(s):  
Gerald L. Vogel ◽  
Gary E. Schumacher ◽  
Laurence C. Chow ◽  
Livia M.A. Tenuta
Keyword(s):  
Sodium Fluoride ◽  
Sodium Lauryl Sulfate ◽  
Lauryl Sulfate ◽  
Fluoride Release ◽  
Important Goal ◽  
Oral Fluids ◽  
Surrogate Measure ◽  
Significant Change ◽  
Topical Fluoride

Increasing the concentration of free fluoride in oral fluids is an important goal in the use of topical fluoride agents. Although sodium lauryl sulfate (SLS) is a common dentifrice ingredient, the influence of this ion on plaque fluid and salivary fluid fluoride has not been examined. The purpose of this study was to investigate the effect of SLS on these parameters and to examine the effect of this ion on total (or whole) plaque fluoride, an important source of plaque fluid fluoride after a sufficient interval following fluoride administration, and on total salivary fluoride, a parameter often used as a surrogate measure of salivary fluid fluoride. Ten subjects accumulated plaque for 48 h before rinsing with a 12 mmol/l NaF (228 µg/g F) rinse containing or not containing 0.5% (w/w) SLS. SLS had no statistically significant effect on total plaque and total saliva fluoride but significantly increased salivary fluid and plaque fluid fluoride (by 147 and 205%, respectively). These results suggest that the nonfluoride components of topical agents can be manipulated to improve the fluoride release characteristics from oral fluoride reservoirs and that statistically significant change may be observed in plaque fluid and salivary fluid fluoride concentrations that may not be observed in total plaque and total saliva fluoride concentrations.

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