Acetylene reduction by non-symbiotic bacteria in artificial soil aggregates amended with glucose
The C2H2-reduction assay was used to study the activity of non-symbiotic N2-fixing bacteria in glucose-amended artificial soil aggregates prepared from a sandy clay loam and a sandy loam soil. Anaerobic incubation under nitrogen resulted in significantly higher rates and greater total quantities of C2H2 reduction than did incubation in air. In the sandy clay loam soil activity under both incubation conditions was due to anaerobic N2-fixing bacteria, whereas in the sandy loam soil aerobic N2-fixing bacteria also proliferated in the aggregates. Direct observation of sectioned aggregates by fluorescence microscopy showed that peripheral zone 1 mm thick sup ported the growth of aerobic microorganisms. The anaerobic microsites in the interior of the aggregates were well protected since disruption procedures involving vigorous shaking with aerated water were required to allow O2 to penetrate and inhibit the anaerobic activity.