β-Xylosidases in the yeast Cryptococcus albidus var. aerius

β-Xylosidase activity has been detected in cell-free extracts and in culture fluids when Cryptococcus albidus var. aerius was grown on glucose as the sole carbon source. The enzyme appears to be constitutive. Mild acid treatment of whole cells suggested that the total activity is located in the periplasmic space and some experiments indicated that it is partially associated with the cell walls. DEAE-Sephadex A50 chromatography has shown that there are two different forms of β-xylosidase in the cell-free extracts, but only one form is present in the supernatants of culture.

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Immunological investigations of Penicillium.: I. Serological reactivities of exocellular polysaccharides produced by six Penicillium species

Canadian Journal of Microbiology ◽

10.1139/m70-118 ◽

1970 ◽

Vol 16 (8) ◽

pp. 687-694 ◽

Cited By ~ 16

Author(s):

J. F. Preston III ◽

Erlinda Lapis ◽

J. E. Gander

Keyword(s):

Acid Hydrolysis ◽

Acid Treatment ◽

Cross Reactivity ◽

Antigenic Specificity ◽

Mild Acid Hydrolysis ◽

Penicillium Species ◽

Whole Cells ◽

Exocellular Polysaccharides ◽

Antigenic Reactivity ◽

Mild Acid

The exocellular polysaccharides of Penicillium charlesii, P. chrysogenum, P. raistrickii, P. claviforme, and P. patulum contain galactofuranosyl residues since galactose is released by mild acid hydrolysis. Antisera from rabbits injected with whole cells of P. charlesii cross reacted with the exocellular polysaccharides from each species. The exocellular polymer from Penicillium various contained no galactose labile to mild acid treatment, and showed no cross reactivity with antisera to P. charlesii. No antigenic reactivity was observed in exocellular polysaccharides modified by mild acid hydrolysis. Oligosaccharides produced by mild acid hydrolysis which contained galactofuranosyl moieties as well as methyl α-D- and methyl β-D-galactofuranosides inhibited precipitate formation when the antigen was incubated with antisera. Treatment of the polysaccharides with 1 N NaOH did not alter its reactivity toward the antisera. It was concluded that several species of Penicillium elaborate exocellular polysaccharides containing galactofuranosyl residues and that these residues contribute to the antigenic specificity of the organism.

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β-Glucanases in the yeast Cryptococcus albidus var. aerius. Production and separation of β-glucanases in asynchronous cultures

Canadian Journal of Microbiology ◽

10.1139/m76-035 ◽

1976 ◽

Vol 22 (2) ◽

pp. 261-268 ◽

Cited By ~ 15

Author(s):

V. Notario ◽

T. G. Villa ◽

T. Benítez ◽

J. R. Villanueva

Keyword(s):

Molecular Weight ◽

Carbon Source ◽

Stationary Phase ◽

Sole Carbon Source ◽

Molecular Form ◽

Cryptococcus Albidus ◽

Glucanase Activity ◽

Maximal Production

β-Glucanases were detected in cell-free extracts of the yeast Cryptococcus albidus var. aerius when grown on glucose as the sole carbon source. The production of β-glucanases was followed in log-phase cells and stationary-phase cells; the maximal production of β-(1 → 3) and β-(1 → 6) glucanases takes place respectively in log-phase and stationary-phase cells. The results show that there are marked differences in the elution profiles on Sephadex G-50 of fractions containing β-glucanase from cells grown for 12, 24, 48, 72, and 96 h. The possibility either of replacement changes in fractions containing β-glucanase activity or of a different synthesis of each β-glucanase during the growth of the yeast is discussed. The results suggest that all fractions containing β-glucanases hydrolyze both β-(1 → ) and β-(1 → 6) linkages.Evidence in support of the conclusion that a low molecular form of β-glucanase has a molecular weight of 2100 ± 100 is also shown.

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The effect of volatile fatty acids as a sole carbon source on lipid accumulation by Cryptococcus albidus for biodiesel production

Bioresource Technology ◽

10.1016/j.biortech.2010.10.141 ◽

2011 ◽

Vol 102 (3) ◽

pp. 2695-2701 ◽

Cited By ~ 172

Author(s):

Qiang Fei ◽

Ho Nam Chang ◽

Longan Shang ◽

Jin-dal-rae Choi ◽

NagJong Kim ◽

...

Keyword(s):

Fatty Acids ◽

Carbon Source ◽

Lipid Accumulation ◽

Sole Carbon Source ◽

Volatile Fatty Acids ◽

Biodiesel Production ◽

Cryptococcus Albidus

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Cytological responses of Cladosporium resinae when shifted from glucose to hydrocarbon medium

Canadian Journal of Microbiology ◽

10.1139/m81-186 ◽

1981 ◽

Vol 27 (11) ◽

pp. 1209-1218 ◽

Cited By ~ 11

Author(s):

R. A. Smucker ◽

J. J. Cooney

Keyword(s):

Carbon Source ◽

Cell Walls ◽

Sole Carbon Source ◽

Glucose Medium ◽

Working Model ◽

Dense Bodies ◽

Hydrocarbon Medium ◽

In Cells

Cladosporium resinae was grown on glucose and then transferred to medium with glucose or with kerosene as the sole carbon source. Growth on hydrocarbon was associated with thinner cell walls in both hyphae and spores, with the presence of large vacuoles in cells, with the synthesis of microbodies, and with increased synthesis of catalase. Some vacuoles in hydrocarbon-grown cells contained small, spherical, electron-dense inclusions which were not observed in cells from glucose medium. Large, electron-dense bodies within vacuoles were observed in glucose-grown and in hydrocarbon-grown cells. A working model is proposed for oxidation of n-alkanes by C. resinae.

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BACTERIAL METABOLISM OFD-ASPARTATE INVOLVING RACEMIZATION AND DECARBOXYLATION

Canadian Journal of Microbiology ◽

10.1139/m66-101 ◽

1966 ◽

Vol 12 (4) ◽

pp. 745-751 ◽

Cited By ~ 6

Author(s):

A. J. Markovetz ◽

W. J. Cook ◽

A. D. Larson

Keyword(s):

Carbon Dioxide ◽

Carbon Source ◽

Sole Carbon Source ◽

Bacterial Metabolism ◽

Alanine Racemase ◽

Whole Cells

A pseudomonad was isolated from soil with D-aspartate serving as sole carbon source. Whole cells and cellular extracts produced carbon dioxide and DL-alanine from D- and L-aspartate. The addition of oxamycin to cellular extracts inhibited the alanine racemase found to be present and it was ascertained that L-alanine was formed from both D- and L-aspartate, indicating that D-aspartate had been racemized to the L-isomer with subsequent decarboxylation to L-alanine.

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Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2020.10.032 ◽

2020 ◽

Author(s):

Vivek Kumar Ranjan ◽

Shriparna Mukherjee ◽

Subarna Thakur ◽

Krutika Gupta ◽

Ranadhir Chakraborty

Keyword(s):

Carbon Source ◽

Sole Carbon Source ◽

New Delhi ◽

Pseudomonas Sp

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Performance enhancement of H2S-based autotrophic denitrification with bio-gaseous CO2 as sole carbon source through new pH adjustment materials

Journal of Environmental Management ◽

10.1016/j.jenvman.2020.110157 ◽

2020 ◽

Vol 261 ◽

pp. 110157 ◽

Cited By ~ 1

Author(s):

Yongjie Liu ◽

Nan Chen ◽

Shuang Tong ◽

Jing Liang ◽

Chen Yang ◽

...

Keyword(s):

Carbon Source ◽

Sole Carbon Source ◽

Performance Enhancement ◽

Autotrophic Denitrification ◽

Ph Adjustment

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Actinomycetes in discolored wood of living silver maple

Canadian Journal of Botany ◽

10.1139/b81-001 ◽

1981 ◽

Vol 59 (1) ◽

pp. 1-7 ◽

Cited By ~ 13

Author(s):

Robert A. Blanchette ◽

John B. Sutherland ◽

Don L. Crawford

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Carbon Source ◽

Cell Walls ◽

Hydroxybenzoic Acid ◽

Liquid Media ◽

Streptomyces Strain ◽

Culture Techniques ◽

Silver Maple ◽

Scanning Electron

The greenish-brown margin of discolored wood in three living silver maple trees, Acer saccharinum L., was examined by scanning electron microscopy and microbiological culture techniques. Micrographs of xylem vessels revealed filamentous structures; some of them appeared to be actinomycetous hyphae. Actinomycetes identified as Streptomyces parvullus Waksman & Gregory, S. sparsogenes Owen, Dietz & Camiener, and a third Streptomyces strain were isolated repeatedly from discolored wood of each tree. These isolates grew in liquid media in the presence of 0.1% (w/v) concentrations of several phenols. Although other phenols included in the test were not substantially degraded, p-hydroxybenzoic acid was utilized as a carbon source by S. parvullus. All three actinomycetes inhibited growth of selected wood-inhabiting fungi when paired on malt agar. When inoculated on sterilized sapwood and discolored wood from silver maple, the actinomycetes colonized vessel walls and occlusions, but were not observed to decay cell walls.

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