Cytological responses of Cladosporium resinae when shifted from glucose to hydrocarbon medium

Cladosporium resinae was grown on glucose and then transferred to medium with glucose or with kerosene as the sole carbon source. Growth on hydrocarbon was associated with thinner cell walls in both hyphae and spores, with the presence of large vacuoles in cells, with the synthesis of microbodies, and with increased synthesis of catalase. Some vacuoles in hydrocarbon-grown cells contained small, spherical, electron-dense inclusions which were not observed in cells from glucose medium. Large, electron-dense bodies within vacuoles were observed in glucose-grown and in hydrocarbon-grown cells. A working model is proposed for oxidation of n-alkanes by C. resinae.

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Lag, adaptation and ageing in a micro-organism ( Bact. lactis aerogenes )

Proceedings of the Royal Society of London Series B Biological Sciences ◽

10.1098/rspb.1961.0065 ◽

1961 ◽

Vol 155 (959) ◽

pp. 195-201 ◽

Cited By ~ 1

Keyword(s):

Carbon Source ◽

Generation Time ◽

Sole Carbon Source ◽

The Other ◽

Lag Phase ◽

Glucose Medium ◽

Galactosidase Activity ◽

Aerobacter Aerogenes ◽

Isomerase Activity

The lag preceding growth of Bact. lactis aerogenes (Aerobacter aerogenes) after a first transfer to a medium containing D-arabinose as sole carbon source increases with the age and decreases with the size of the inoculum. During the long lag phase the β -galactosidase activity declines steeply. In contrast with this (and with a control ageing in a glucose medium) the D-ribulose isomerase activity is maintained, although no detectable consumption of D-arabinose occurs. If the long lag of unadapted cells in D-arabinose is divided into parts by intermediate passages in glucose or lactose media, the sum of the partial lags is nearly constant and equal to that observed when there is no interruption. But the periodic passages in the other media increase the rate at which growth eventually occurs in the D-arabinose. It is concluded that during the lag a decay of the enzymes in general occurs concomitantly with the development of the specific mechanisms concerned in the utilization of the new substrate. The balance of these processes (together with varying loss or retention of diffusible metabolites) is largely responsible for the observed variations in lag and mean generation time.

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Azotobacter vinelandii Aldehyde Dehydrogenase Regulated by ς54: Role in Alcohol Catabolism and Encystment

Journal of Bacteriology ◽

10.1128/jb.183.21.6169-6174.2001 ◽

2001 ◽

Vol 183 (21) ◽

pp. 6169-6174 ◽

Cited By ~ 5

Author(s):

Socorro Gama-Castro ◽

Cinthia Núñez ◽

Daniel Segura ◽

Soledad Moreno ◽

Josefina Guzmán ◽

...

Keyword(s):

Carbon Source ◽

Aldehyde Dehydrogenase ◽

Bacterial Growth ◽

Sole Carbon Source ◽

Azotobacter Vinelandii ◽

Gene Encoding ◽

In Cells

ABSTRACT Encystment in Azotobacter vinelandii is induced byn-butanol or β-hydroxybutyrate (BHB). We identified a gene, encoding an aldehyde dehydrogenase, that was namedaldA. An aldA mutation impaired bacterial growth on n-butanol, ethanol, or hexanol as the sole carbon source. Expression of aldA increased in cells shifted from sucrose to n-butanol and was shown to be dependent on the alternative ς54 factor. A mutation in rpoNencoding the ς54 factor also impaired growth on alcohols. Encystment on n-butanol, but not on BHB, was impaired inaldA or rpoN mutants, indicating thatn-butanol is not an inducer of encystment by itself but must be catabolized in order to induce encystment.

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Saccharomyces cerevisiae glucose signalling regulator Mth1p regulates the organellar Na+/H+ exchanger Nhx1p

Biochemical Journal ◽

10.1042/bj20100796 ◽

2010 ◽

Vol 432 (2) ◽

pp. 343-352 ◽

Cited By ~ 2

Author(s):

Keiji Mitsui ◽

Masafumi Matsushita ◽

Hiroshi Kanazawa

Keyword(s):

Saccharomyces Cerevisiae ◽

Carbon Source ◽

Sole Carbon Source ◽

Glucose Sensor ◽

Ph Regulation ◽

Gene Knockout ◽

Carbon Sources ◽

Acidic Ph ◽

In Cells

Organelle-localized NHEs (Na+/H+ exchangers) are found in cells from yeast to humans and contribute to organellar pH regulation by exporting H+ from the lumen to the cytosol coupled to an H+ gradient established by vacuolar H+-ATPase. The mechanisms underlying the regulation of organellar NHEs are largely unknown. In the present study, a yeast two-hybrid assay identified Mth1p as a new binding protein for Nhx1p, an organellar NHE in Saccharomyces cerevisiae. It was shown by an in vitro pull-down assay that Mth1p bound to the hydrophilic C-terminal half of Nhx1p, especially to the central portion of this region. Mth1p is known to bind to the cytoplasmic domain of the glucose sensor Snf3p/Rgt2p and also functions as a negative transcriptional regulator. Mth1p was expressed in cells grown in a medium containing galactose, but was lost (possibly degraded) when cells were grown in medium containing glucose as the sole carbon source. Deletion of the MTH1 gene increased cell growth compared with the wild-type when cells were grown in a medium containing galactose and with hygromycin or at an acidic pH. This resistance to hygromycin or acidic conditions was not observed for cells grown with glucose as the sole carbon source. Gene knockout of NHX1 increased the sensitivity to hygromycin and acidic pH. The increased resistance to hygromycin was reproduced by truncation of the Mth1p-binding region in Nhx1p. These results implicate Mth1p as a novel regulator of Nhx1p that responds to specific extracellular carbon sources.

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β-Xylosidases in the yeast Cryptococcus albidus var. aerius

Canadian Journal of Microbiology ◽

10.1139/m76-044 ◽

1976 ◽

Vol 22 (2) ◽

pp. 312-315 ◽

Cited By ~ 12

Author(s):

V. Notario ◽

T. G. Villa ◽

J. R. Villanueva

Keyword(s):

Carbon Source ◽

Cell Walls ◽

Acid Treatment ◽

Sole Carbon Source ◽

Total Activity ◽

Periplasmic Space ◽

Cryptococcus Albidus ◽

Whole Cells ◽

Mild Acid ◽

Xylosidase Activity

β-Xylosidase activity has been detected in cell-free extracts and in culture fluids when Cryptococcus albidus var. aerius was grown on glucose as the sole carbon source. The enzyme appears to be constitutive. Mild acid treatment of whole cells suggested that the total activity is located in the periplasmic space and some experiments indicated that it is partially associated with the cell walls. DEAE-Sephadex A50 chromatography has shown that there are two different forms of β-xylosidase in the cell-free extracts, but only one form is present in the supernatants of culture.

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Identification, Source, and Metabolism of N-Ethylglutamate in Escherichia coli

Journal of Bacteriology ◽

10.1128/jb.00721-10 ◽

2010 ◽

Vol 192 (20) ◽

pp. 5437-5440 ◽

Cited By ~ 1

Author(s):

Robert H. White

Keyword(s):

Escherichia Coli ◽

Carbon Source ◽

Yeast Extract ◽

Sole Carbon Source ◽

Isotope Dilution Analysis ◽

Sole Nitrogen Source ◽

Glucose Medium ◽

Detectable Amount ◽

Deuterated Water ◽

E Coli

ABSTRACT N-Ethylglutamate (NEG) was detected in Escherichia coli BL21 cells grown on LB broth, and it was found to occur at a concentration of ∼4 mM in these cells under these conditions. The same cells grown on M9 glucose medium contained no detectable amount of NEG. Analysis of the LB broth showed the presence of NEG, a compound never before reported as a natural product. Isotope dilution analysis showed that it occurred at a concentration of 160 μM in LB broth. Analyses of yeast extract and tryptone, the organic components of LB broth, both showed the presence NEG. It was demonstrated that NEG can be generated during the autolysis of the yeast used in the preparation of the yeast extract. Growth of these E. coli cells in LB broth prepared in deuterated water showed no incorporation of deuterium into NEG, demonstrating that E. coli cells did not generate the NEG. Cell growth rates were not affected by the addition of 5 mM NEG to either LB or M9 glucose medium. l-[ethyl-2H4]NEG was found to be readily incorporated into the cells and metabolized by the cells. From these results, it was concluded that all of the NEG present in the cells was taken up from the medium. NEG could serve as the sole nitrogen source for E. coli when grown on M9 glucose medium in the presence of glucose but could not serve as the sole carbon source on M9 medium in the absence of glucose.

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Pyruvate fermentation by Clostridium acetobutylicum

Biochemistry and Cell Biology ◽

10.1139/o89-110 ◽

1989 ◽

Vol 67 (10) ◽

pp. 735-739 ◽

Cited By ~ 9

Author(s):

Rachid Janati-Idrissi ◽

Anne-Marie Junelles ◽

Abdellah El Kanouni ◽

Henri Petitdemange ◽

Robert Gay

Keyword(s):

Clostridium Acetobutylicum ◽

Sole Carbon Source ◽

Glucose Medium ◽

Pyruvate Fermentation ◽

End Products ◽

Acetoacetate Decarboxylase ◽

Solvent Formation ◽

Butyrate Kinase ◽

Clostridium Acetobutylicum Atcc 824 ◽

In Cells

Clostridium acetobutylicum ATCC 824 using pyruvate as the sole carbon source produced mainly acetate and butyrate as end products of fermentation. Acetate and butyrate kinase activities were higher in cells growing in the presence of pyruvate than glucose, whereas the level of the acetoacetate decarboxylase, an enzyme involved in solvent formation, was lower. Similar activities of glyceraldehyde-3-phosphate dehydrogenase were found in cells grown in pyruvate and glucose mediums. The transfer of C. acetobutylicum from pyruvate to glucose medium suggested that pyruvate represses the "solventogenesis."Key words: Clostridium acetobutylicum, pyruvic acid, kinase, acetoacetate decarboxylase.

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Pandrug-resistant Pseudomonas sp. expresses New Delhi metallo-β-lactamase-1 and consumes ampicillin as sole carbon source

Clinical Microbiology and Infection ◽

10.1016/j.cmi.2020.10.032 ◽

2020 ◽

Author(s):

Vivek Kumar Ranjan ◽

Shriparna Mukherjee ◽

Subarna Thakur ◽

Krutika Gupta ◽

Ranadhir Chakraborty

Keyword(s):

Carbon Source ◽

Sole Carbon Source ◽

New Delhi ◽

Pseudomonas Sp

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Performance enhancement of H2S-based autotrophic denitrification with bio-gaseous CO2 as sole carbon source through new pH adjustment materials

Journal of Environmental Management ◽

10.1016/j.jenvman.2020.110157 ◽

2020 ◽

Vol 261 ◽

pp. 110157 ◽

Cited By ~ 1

Author(s):

Yongjie Liu ◽

Nan Chen ◽

Shuang Tong ◽

Jing Liang ◽

Chen Yang ◽

...

Keyword(s):

Carbon Source ◽

Sole Carbon Source ◽

Performance Enhancement ◽

Autotrophic Denitrification ◽

Ph Adjustment

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Actinomycetes in discolored wood of living silver maple

Canadian Journal of Botany ◽

10.1139/b81-001 ◽

1981 ◽

Vol 59 (1) ◽

pp. 1-7 ◽

Cited By ~ 13

Author(s):

Robert A. Blanchette ◽

John B. Sutherland ◽

Don L. Crawford

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Carbon Source ◽

Cell Walls ◽

Hydroxybenzoic Acid ◽

Liquid Media ◽

Streptomyces Strain ◽

Culture Techniques ◽

Silver Maple ◽

Scanning Electron

The greenish-brown margin of discolored wood in three living silver maple trees, Acer saccharinum L., was examined by scanning electron microscopy and microbiological culture techniques. Micrographs of xylem vessels revealed filamentous structures; some of them appeared to be actinomycetous hyphae. Actinomycetes identified as Streptomyces parvullus Waksman & Gregory, S. sparsogenes Owen, Dietz & Camiener, and a third Streptomyces strain were isolated repeatedly from discolored wood of each tree. These isolates grew in liquid media in the presence of 0.1% (w/v) concentrations of several phenols. Although other phenols included in the test were not substantially degraded, p-hydroxybenzoic acid was utilized as a carbon source by S. parvullus. All three actinomycetes inhibited growth of selected wood-inhabiting fungi when paired on malt agar. When inoculated on sterilized sapwood and discolored wood from silver maple, the actinomycetes colonized vessel walls and occlusions, but were not observed to decay cell walls.

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