Production of fumigaclavine A by Aspergillus tamarii Kita.

1984 ◽  
Vol 30 (2) ◽  
pp. 247-250 ◽  
Author(s):  
K. K. Janardhanan ◽  
Abdul Sattar ◽  
Akhtar Husain
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Ergot Alkaloid ◽  
Submerged Culture ◽  
High Performance ◽  
Ergot Alkaloids ◽  
Dry Weight ◽  
Aspergillus Tamarii ◽  
Total Alkaloids

Aspergillus tamarii Kita. isolated from seeds of Paspalum scrobiculatum L. is found to produce ergot alkaloids in submerged culture. The cultrue filtrate and mycelium are observed to contain 0.125 mg/mL and 1.2 mg/g (dry weight) total alkaloids consisting of 86.5 and 91.3% fumigaclavine A, respectively. The identification of the compound was confirmed by high-performance liquid chromatography, ultraviolet, infrared, and mass spectrophotometry analyses. This is the first report of the production of ergot alkaloid by this fungus. The possible role of fumigaclavine A as a mycotoxin is discussed.

Role of Hydrogen Peroxide in the Prevention of Growth and Aflatoxin Production by Aspergillus parasiticus

1988 ◽  
Vol 51 (4) ◽  
pp. 263-268 ◽  
Author(s):  
FATHY E. EL-GAZZAR ◽  
ELMER H. MARTH
Keyword(s):  
Hydrogen Peroxide ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Aspergillus Parasiticus ◽  
Dry Weight ◽  
Aflatoxin Production ◽  
Reversed Phase ◽  
And Control

Hydrogen peroxide, 0.0, 0.03, 0.05, 0.3 and 0.5% was added to 25 ml of a glucose-yeast-salts medium which was inoculated with 1 ml of a spore suspension containing 106 conidia of Aspergillus parasiticus NRRL 2999 and then was incubated at 14 or 28°C. Cultures held at 28°C were analyzed after 3, 7 and 10 d for mycelial dry weight, pH and accumulation of aflatoxin B1 and G1. Incubation of some cultures at 28°C was continued for 90 d. Cultures held at 14°C were analyzed after 1, 2 and 3 months for mycelial dry weight, pH and aflatoxin production. Amounts of aflatoxin produced were determined using reversed-phase high-performance liquid chromatography (HPLC). The percentage of inhibition or stimulation by the additive was used to make comparisons between treatments and control. Overall, increasing the concentration of hydrogen peroxide to 0.3 or 0.5% completely prevented growth and aflatoxin production for up to 90 d of incubation at 14 or 28°C.

The role of the temperature in reversed-phase high-performance liquid chromatography using pyrocarbon-containing adsorbents

1978 ◽  
Vol 167 ◽  
pp. 41-65 ◽  
Author(s):  
Henri Colin ◽  
José Carlos Diez-Masa ◽  
Georges Guiochon ◽  
Teresa Czjkowska ◽  
Iréna Miedziak
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Reversed Phase

HPLC Method for Determination of Ergot Alkaloids and Some Derivatives in Human Plasma

1985 ◽  
Vol 4 (6) ◽  
pp. 601-607 ◽  
Author(s):  
M. Žorž ◽  
J. Culig ◽  
Z. Kopitar ◽  
D. Milivojevic ◽  
A. Marušič ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Human Plasma ◽  
High Performance ◽  
Ergot Alkaloids ◽  
Hplc Method ◽  
Fluorometric Detection

1 Ergot alkaloids and their dihydrogenated methanesulphonate (ms) salts were determined and measured in human plasma. 2 High-performance liquid chromatography (HPLC) with fluorometric detection was used for separation of ergot alkaloids in plasma. 3 Several ergot alkaloids and their derivatives, including lysergide (LSD), can be identified in cases of poisoning.

scholarly journals Variations in Anthocyanin Profiles and Antioxidant Activity of 12 Genotypes of Mulberry (Morus spp.) Fruits and Their Changes during Processing

Antioxidants ◽  
2020 ◽  
Vol 9 (3) ◽  
pp. 242 ◽  
Author(s):  
Inhwan Kim ◽  
Jihyun Lee
Keyword(s):  
Antioxidant Activity ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Citric Acid ◽  
High Performance ◽  
Antioxidant Activities ◽  
Dry Weight ◽  
Mulberry Fruit ◽  
Mulberry Fruits ◽  
Anthocyanin Composition

Mulberry fruits are known as rich sources of anthocyanins and are consumed in syrup form after the addition of sugar and acid; however, there is little information on the anthocyanin composition and antioxidant activity of mulberries of different cultivars and their changes during processing. To address this, the antioxidant activity and anthocyanin composition of 12 cultivar mulberry fruit cultivars were investigated by high-performance liquid chromatography and ultra-high-performance liquid chromatography coupled with electrospray ionization/quadrupole time-of-flight. Additionally, different quantities of citric acid were used to evaluate antioxidant activities and anthocyanin composition of mulberry syrup. Sixteen anthocyanins were identified in mulberry fruits using accurate mass spectrometry. Several anthocyanins were tentatively identified for the first time in mulberry fruits and include: malvidin hexoside, cyanidin malonyl hexose hexoside, cyanidin pentoside, cyanidin malonyl hexoside, petunidin deoxyhexose hexoside, and cyanidin deoxyhexoside. The major anthocyanin in mulberries was cyanidin-3-O-glucoside, followed by cyanidin-3-O-rutinoside. Morus Alba L. Iksu showed the highest cyanidin-3-O-glucoside content (8.65 mg/g dry weight) among 12 mulberry fruit cultivars. As citric acid levels increased, mulberry syrup showed significantly higher antioxidant activity (p < 0.05).

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