The effects of varying moisture and nutrient levels on the transfer of a conjugative plasmid between Streptomyces species in soil

1989 ◽  
Vol 35 (5) ◽  
pp. 544-549 ◽  
Author(s):  
Bruce H. Bleakley ◽  
Don L. Crawford
Keyword(s):  
Selective Medium ◽  
Plasmid Transfer ◽  
Fluorescein Diacetate ◽  
Conjugative Plasmid ◽  
Cottonseed Flour ◽  
Streptomyces Species ◽  
Moisture Treatment ◽  
Fluorescein Diacetate Activity ◽  
Nutrient Amendment ◽  
Water Holding

Biparental crosses were established in sterile silt loam between a plasmid-free Streptomyces parvulus strain (recipient) and a recombinant Streptomyces lividans strain (donor) bearing the recombinant, conjugational plasmid pIJ303 which codes for thiostrepton resistance. The crosses were established in autoclaved portions of soil adjusted to approximately 60, 40, and 20% water-holding capacity. The soil was amended with either CaCO3 alone (limed), or CaCO3 along with cottonseed flour, chitin, and cellulose (nutrient amended). After a month or longer incubation at 30 °C, the number of transconjugants in each soil treatment was determined by spread-plating on thiostrepton–agar selective medium. The heterotrophic microbial activity of each soil was also assayed with fluorescein diacetate. Nutrient amendment resulted in two to three times more fluorescein diacetate activity than for the unamended, limed treatments of equivalent moisture content. The nutrient-amended, low moisture treatment resulted in the greatest frequency of plasmid transfer. In comparison, plasmid transfer frequencies for the nutrient-amended, higher moisture treatments and for the limed treatments were approximately 5 to 73 times lower. The results suggest that nutrient-amended, relatively dry soils possess frequent microsites where mycelial growth and conjugationally mediated plasmid exchange between streptomycetes occur readily.Key words: Streptomyces, plasmid, conjugation, soil, gene transfer.

scholarly journals Conjugative plasmid transfer from Escherichia coli to Clostridium acetobutylicum

1990 ◽  
Vol 136 (5) ◽  
pp. 819-826 ◽  
Author(s):  
D. R. Williams ◽  
D. I. Young ◽  
M. Young
Keyword(s):  
Escherichia Coli ◽  
Clostridium Acetobutylicum ◽  
Plasmid Transfer ◽  
Conjugative Plasmid ◽  
Conjugative Plasmid Transfer

Conjugative plasmid transfer betweenPseudomonas strains within alginate bead microcosms: Effect of the internal gel structure

1999 ◽  
Vol 65 (1) ◽  
pp. 34-43 ◽  
Author(s):  
Denis D.G. Mater ◽  
Jos� E. Nava Saucedo ◽  
Nicole Truffaut ◽  
Jean-No�l Barbotin ◽  
Daniel Thomas
Keyword(s):  
Alginate Bead ◽  
Plasmid Transfer ◽  
Conjugative Plasmid ◽  
Gel Structure ◽  
Conjugative Plasmid Transfer

R-plasmid transfer in soil and water

1986 ◽  
Vol 32 (7) ◽  
pp. 610-613 ◽  
Author(s):  
J. T. Trevors ◽  
K. M. Oddie
Keyword(s):  
Escherichia Coli ◽  
Stream Water ◽  
Plasmid Transfer ◽  
Nutrient Broth ◽  
Water Holding Capacity ◽  
Sterile Soil ◽  
Water Holding ◽  
Soil And Water

R-plasmid transfer in Escherichia coli was investigated in nutrient broth, sterile soil, and sterile stream water. Plasmid transfer occurred in broth cultures at 30 and 22 °C, but not at 15 °C. R-plasmid transfer was not observed at 30, 22, and 15 °C in nonamended sterile soil and stream water. The addition of nutrients to sterile stream water and soil allowed plasmid transfer to occur at temperatures ranging from 15 to 30 °C. R-plasmid transfer was also observed in soil adjusted from 20 to 100% of its water-holding capacity.

scholarly journals Dissection of IncP conjugative plasmid transfer: definition of the transfer region Tra2 by mobilization of the Tra1 region in trans.

1992 ◽  
Vol 174 (8) ◽  
pp. 2493-2500 ◽  
Author(s):  
M Lessl ◽  
D Balzer ◽  
R Lurz ◽  
V L Waters ◽  
D G Guiney ◽  
...  
Keyword(s):  
Plasmid Transfer ◽  
Conjugative Plasmid ◽  
Conjugative Plasmid Transfer ◽  
Transfer Region ◽  
In Trans ◽  
Definition Of

scholarly journals Molecular Survey of the Dissemination of TwoblaKPC-Harboring IncFIA Plasmids in New Jersey and New York Hospitals

2014 ◽  
Vol 58 (4) ◽  
pp. 2289-2294 ◽  
Author(s):  
Liang Chen ◽  
Kalyan D. Chavda ◽  
Roberto G. Melano ◽  
Tao Hong ◽  
Albert D. Rojtman ◽  
...  
Keyword(s):  
Health Care ◽  
New York ◽  
New Jersey ◽  
Plasmid Transfer ◽  
Health Care Facilities ◽  
Conjugative Plasmid ◽  
Content Type ◽  
Care Facilities ◽  
Clonal Spread ◽  
Sequence Types

ABSTRACTKlebsiella pneumoniaecarbapenemase (KPC)-producingK. pneumoniaestrains have spread worldwide and become a major threat in health care facilities. Transmission ofblaKPC, the plasmid-borne KPC gene, can be mediated by clonal spread and horizontal transfer. Here, we report the complete nucleotide sequences of two novelblaKPC-3-harboring IncFIA plasmids, pBK30661 and pBK30683. pBK30661 is 74 kb in length, with a mosaic plasmid structure; it exhibits homologies to several other plasmids but lacks the plasmid transfer operon (tra) and the origin of transfer (oriT) that are required for plasmid transfer. pBK30683 is a conjugative plasmid with a cointegrated plasmid structure, comprising a 72-kb element that highly resembles pBK30661 (>99.9% nucleotide identities) and an extra 68-kb element that harborstraandoriT. A PCR scheme was designed to detect the distribution ofblaKPC-harboring IncFIA (pBK30661-like and pBK30683-like) plasmids in a collection of clinicalEnterobacteriaceaeisolates from 10 hospitals in New Jersey and New York. KPC-harboring IncFIA plasmids were found in 20% of 491K. pneumoniaeisolates, and all carriedblaKPC-3. pBK30661-like plasmids were identified mainly in the epidemic sequence type 258 (ST258)K. pneumoniaeclone, while pBK30683-like plasmids were widely distributed in ST258 and otherK. pneumoniaesequence types and among non-K. pneumoniae Enterobacteriaceaespecies. This suggests that both clonal spread and horizontal plasmid transfer contributed to the dissemination ofblaKPC-harboring IncFIA plasmids in our area. Further studies are needed to understand the distribution of this plasmid group in other health care regions and to decipher the origins of pBK30661-like and pBK30683-like plasmids.

scholarly journals Conjugative Plasmid Transfer in the Biofilm Formed by Enterococcus faecalis

2006 ◽  
Vol 52 (4) ◽  
pp. 358-367 ◽  
Author(s):  
Takumi Kajiura ◽  
Hideki Wada ◽  
Kenji Ito ◽  
Yojiro Anzai ◽  
Fumio Kato
Keyword(s):  
Enterococcus Faecalis ◽  
Plasmid Transfer ◽  
Conjugative Plasmid ◽  
Conjugative Plasmid Transfer

Rule-based modelling of conjugative plasmid transfer and incompatibility

Biosystems ◽  
2008 ◽  
Vol 91 (1) ◽  
pp. 201-215 ◽  
Author(s):  
R. Gregory ◽  
J.R. Saunders ◽  
V.A. Saunders
Keyword(s):  
Plasmid Transfer ◽  
Conjugative Plasmid ◽  
Rule Based ◽  
Conjugative Plasmid Transfer

scholarly journals Genetic Analysis of the Enterococcus Vancomycin Resistance Conjugative Plasmid pHTβ: Identification of the Region Involved in Cell Aggregation and traB, a Key Regulator Gene for Plasmid Transfer and Cell Aggregation

2008 ◽  
Vol 190 (23) ◽  
pp. 7739-7753 ◽  
Author(s):  
Haruyoshi Tomita ◽  
Yasuyoshi Ike
Keyword(s):  
Deletion Mutant ◽  
Cell Aggregation ◽  
Vancomycin Resistance ◽  
Plasmid Transfer ◽  
Open Reading Frames ◽  
Conjugative Plasmid ◽  
Host Strain ◽  
Transcription Analysis ◽  
Transfer Frequency ◽  
Reading Frames

ABSTRACT The Enterococcus plasmid pHTβ (63.7 kbp) is a pheromone-independent, highly conjugative pMG1-like plasmid that carries a Tn1546-like transposon encoding vancomycin resistance. The transfer-related regions (Tra I, Tra II, and Tra III) containing oriT and a putative nickase gene (traI) have previously been identified in pHTβ, and in this study, we found that the plasmid conferred the ability to self-aggregate on the host strain Enterococcus faecalis FA2-2. A region where mutation resulted in the impairment of aggregation was identified and mapped to a point upstream of the transfer-related Tra I region. This region consisted of an approximately 6-kbp segment that contained the five open reading frames (ORFs) ORF9 to ORF13. These ORFs are considered to encode the aggregation function, although the precise mode of action of each ORF has not yet been elucidated. An in-frame deletion mutant of ORF10 resulted in reduced aggregation and decreased transfer frequency in broth mating. Transcription analysis of the aggregation region showed that the five ORFs from ORF9 to ORF13 form an operon structure, and a long transcript that started from a promoter region located upstream of ORF9 was identified. Tra II spans a 1.7-kbp region containing ORF56 and ORF57. Tn917-lac insertions into or an in-frame deletion mutant of ORF56 (187 amino acids) resulted in impaired transfer and aggregation. The cloned ORF56 complemented these functions in trans. The transcription levels of ORF10 and ORF13 were reduced in the in-frame mutants of ORF56, but this reduction was complemented by a cloned ORF56 in trans. The results indicated that ORF56 positively regulated the aggregation and plasmid transfer in the host strain, and ORF56 was designated traB.

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