Acidiphilium symbioticum sp.nov., an acidophilic heterotrophic bacterium from Thiobacillus ferrooxidans cultures isolated from Indian mines

1991 ◽  
Vol 37 (1) ◽  
pp. 78-85 ◽  
Author(s):  
Saswati Bhattacharyya ◽  
B. K. Chakrabarty ◽  
A Das ◽  
P. N. Kundu ◽  
P. C. Banerjee
Keyword(s):  
Yeast Extract ◽  
Thiobacillus Ferrooxidans ◽  
Heterotrophic Bacterium ◽  
Sole Source ◽  
Striking Similarity ◽  
Mineral Salts ◽  
Acidophilic Bacteria ◽  
Bacterial Contaminants ◽  
Acidiphilium Cryptum ◽  
Yeast Extract Medium

Two cultures of Thiobacillus ferrooxidans enriched from Indian mine samples and grown autotrophically on FeSO4 – basal salts medium for periods ranging from several months to years contained acidophilic, heterotrophic bacterial contaminants. The heterotrophs (strains KM2 and H8) were isolated by selective growth in a mineral salts – glucose – yeast extract medium of pH 3 and were purified as single colonies on an agarose medium. Mannose, galactose, sucrose, lactose, citrate, mannitol, and glycerol supported the growth of these strains in the presence of yeast extract. The heterotrophs grew poorly or failed to grow in media without yeast extract. They could not grow autotrophically with Fe2+, or with sulfur and its oxidizable derivatives, as the sole source of energy. Although they exhibited many characteristics of the genus Acidiphilium, they differed from Acidiphilium cryptum and other species of this genus in some physiological properties, notably in their ability to grow at higher glucose (5%, w/v) and Mn2+ (20 mM) concentrations. The G+C mol% contents (58.8 and 60.2) of strains KM2 and H8, respectively, determined from melting temperature (Tm) values were close to that of A. cryptum (62.7%). Strains KM2 and H8 showed 70–80% DNA homology with each other and about 60% with A. cryptum. All of the strains, including A. cryptum, responded similarly to several metal ions and antibiotics. SDS–PAGE of whole-cell proteins exhibited striking similarity between these two isolated strains, which were unlike that of A. cryptum. The strains were also agglutinated with a few common lectins and differed strongly from A. cryptum in respect to wheat-germ agglutinin and concanavalin A. Considering all these characteristics, we propose that strains KM2 and H8 be designated as a new species: Acidiphilium symbioticum. The type strain of A. symbioticum is strain KM2 (= MTCC 566). Key words: Acidiphilium symbioticum, Acidiphilium cryptum, Thiobacillus ferrooxidans, acidophilic bacteria.

Leaching of Pyrite by Acidophilic Heterotrophic Iron-Oxidizing Bacteria in Pure and Mixed Cultures

1999 ◽  
Vol 65 (2) ◽  
pp. 585-590 ◽  
Author(s):  
Paula Bacelar-Nicolau ◽  
D. Barrie Johnson
Keyword(s):  
Yeast Extract ◽  
Thiobacillus Ferrooxidans ◽  
Pyrite Oxidation ◽  
Mixed Cultures ◽  
Mineral Dissolution ◽  
Acidophilic Bacteria ◽  
Pure Cultures ◽  
Sulfur Oxidizing ◽  
Thiobacillus Acidophilus ◽  
Basal Salts

ABSTRACT Seven strains of heterotrophic iron-oxidizing acidophilic bacteria were examined to determine their abilities to promote oxidative dissolution of pyrite (FeS2) when they were grown in pure cultures and in mixed cultures with sulfur-oxidizingThiobacillus spp. Only one of the isolates (strain T-24) oxidized pyrite when it was grown in pyrite-basal salts medium. However, when pyrite-containing cultures were supplemented with 0.02% (wt/vol) yeast extract, most of the isolates oxidized pyrite, and one (strain T-24) promoted rates of mineral dissolution similar to the rates observed with the iron-oxidizing autotroph Thiobacillus ferrooxidans. Pyrite oxidation by another isolate (strain T-21) occurred in cultures containing between 0.005 and 0.05% (wt/vol) yeast extract but was completely inhibited in cultures containing 0.5% yeast extract. Ferrous iron was also needed for mineral dissolution by the iron-oxidizing heterotrophs, indicating that these organisms oxidize pyrite via the “indirect” mechanism. Mixed cultures of three isolates (strains T-21, T-23, and T-24) and the sulfur-oxidizing autotroph Thiobacillus thiooxidans promoted pyrite dissolution; since neither strains T-21 and T-23 nor T. thiooxidans could oxidize this mineral in yeast extract-free media, this was a novel example of bacterial synergism. Mixed cultures of strains T-21 and T-23 and the sulfur-oxidizing mixotrophThiobacillus acidophilus also oxidized pyrite but to a lesser extent than did mixed cultures containing T. thiooxidans. Pyrite leaching by strain T-23 grown in an organic compound-rich medium and incubated either shaken or unshaken was also assessed. The potential environmental significance of iron-oxidizing heterotrophs in accelerating pyrite oxidation is discussed.

Thermal injury and death in an obligately psychrophilic yeast, Candida nivalis

1968 ◽  
Vol 14 (6) ◽  
pp. 691-697 ◽  
Author(s):  
C. H. Nash ◽  
N. A. Sinclair
Keyword(s):  
Yeast Extract ◽  
Growth Temperature ◽  
Membrane Damage ◽  
Maximal Growth ◽  
Rapid Loss ◽  
Mineral Salts ◽  
Psychrophilic Yeast ◽  
Yeast Extract Medium ◽  
Cellular Components ◽  
Component Addition

Exposure of the obligately psychrophilic yeast, Candida nivalis, to temperatures greater than 20 C, its maximal growth temperature, results in a rapid loss in viability. Furthermore, many of the cells surviving heat treatment are metabolically injured. Injured cells are unable to develop on a glucose – mineral salts – vitamins medium but are able to develop on a complex tryptone – glucose – yeast extract medium. Recovery of injured cells on the complex medium is due to the yeast extract component. Addition of cysteine, reduced glutathione, or thioglycollate to the minimal medium also enhances recovery of heat-injured cells.Additional evidence of heat-induced damage is the release of various cellular components into the suspending menstruum. These include inorganic phosphate, amino acids or short polypeptides, and nucleotide monophosphate. Leakage of these materials into the heating menstruum is not due to cell lysis. The correlation between leakage and loss in viability suggests that membrane damage is at least one factor which determines the low maximal growth temperature of C. nivalis.

NUTRITION AND METABOLISM OF MARINE BACTERIA: X. THE GLYOXYLATE CYCLE IN A MARINE BACTERIUM

1960 ◽  
Vol 6 (6) ◽  
pp. 639-644 ◽  
Author(s):  
R. A. MacLeod ◽  
Aiko Hori ◽  
Sylvia M. Fox
Keyword(s):  
Yeast Extract ◽  
Marine Bacteria ◽  
Marine Bacterium ◽  
Glyoxylate Cycle ◽  
Sole Source ◽  
Nutrient Broth ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
The Glyoxylate Cycle

Extracts of a species of marine bacterium have been shown to contain isocitratase and malate synthetase when cells were grown in a medium in which acetate was the sole source of carbon and energy. Neither enzyme could be demonstrated in extracts prepared from cells grown in a nutrient broth, yeast extract medium.

Isolation and identification of N2-fixing Pseudomonas associated with wetland rice

1983 ◽  
Vol 29 (8) ◽  
pp. 867-873 ◽  
Author(s):  
W. L. Barraquio ◽  
J. K. Ladha ◽  
I. Watanabe
Keyword(s):  
New Species ◽  
Yeast Extract ◽  
Heterotrophic Bacteria ◽  
Fluorescent Antibody ◽  
Wetland Rice ◽  
Isolation And Identification ◽  
Biochemical Characteristics ◽  
Yeast Extract Medium ◽  
Extract Medium ◽  
A New Species

Semisolid yeast extract medium amended with glucose and tryptic soy agar were used to isolate aerobically N2-fixing (C2H2-reducing) heterotrophic bacteria from the root of wetland rice. The isolates were identified as Pseudomonas by gel immunodiffusion and fluorescent antibody techniques in combination with their morphological, cultural, and biochemical characteristics. The N2-fixing H2-utilizing Pseudomonas described in this paper is a new species.

STRAIN IMPROVEMENT OF A FUNGUS PRODUCING CHITINASE BY A CHEMICAL MUTAGEN

INDIAN DRUGS ◽  
2013 ◽  
Vol 50 (11) ◽  
pp. 25-28
Author(s):  
K Narayanan ◽  
◽  
N.D. Chopade ◽  
V.M Subrahmanyam ◽  
J. Venkata Rao
Keyword(s):  
Protein Content ◽  
Ethidium Bromide ◽  
Yeast Extract ◽  
Wild Strain ◽  
Strain Improvement ◽  
Cost Effective ◽  
Specific Protein ◽  
Chemical Mutagen ◽  
Yeast Extract Medium ◽  
Extract Medium

Microbial chitinases are commercially exploited for their biocontrol properties and generation of useful products from chitinous waste. Availability of highly active chitinolytic enzymes is a major problem. The present study was carried out to improve chitinase production by Aspergillus terreus using a chemical mutagen, ethidium bromide. The organism was cultivated on lactose- yeast extract medium. The production medium consisting of chitin- yeast extract medium was seeded at 10% level. The wild strains were exposed to ethidium bromide in the concentration range 1.5- 6.0 µg/mL. Generally, all the mutated strains showed an improved chitinase yield compared to the control. Highest yield was observed with the strain exposed to 6 µg/mL of ethidium bromide. The yield was 25.03 % higher compared to the wild strain. The mutated strain was slimy in nature. Protein content of the mutated strain decreased by 11%. Ethidium bromide at a concentration of 1.5 µg/mL was considered optional, at which the strain was stable with increase of 21.80 % in enzyme activity and 4.41% increase in protein content. Increased enzyme yield with decreased non-specific protein could be useful in producing cost effective enzyme.

Inducible aluminum resistance of Acidiphilium cryptum and aluminum tolerance of other acidophilic bacteria

2002 ◽  
Vol 178 (6) ◽  
pp. 554-558 ◽  
Author(s):  
Jörg Fischer ◽  
Armin Quentmeier ◽  
Sven Gansel ◽  
Vera Sabados ◽  
Cornelius Friedrich
Keyword(s):  
Aluminum Tolerance ◽  
Aluminum Resistance ◽  
Acidophilic Bacteria ◽  
Acidiphilium Cryptum

In vitro growth of wheat and flax rust fungi on complex and chemically defined media

1974 ◽  
Vol 52 (6) ◽  
pp. 1183-1195 ◽  
Author(s):  
A. Bose ◽  
Michael Shaw
Keyword(s):  
Liquid Medium ◽  
Aspartic Acid ◽  
Yeast Extract ◽  
Australian Isolate ◽  
Puccinia Graminis ◽  
Good Growth ◽  
Complex Media ◽  
Mineral Salts ◽  
Melampsora Lini

Growth from uredospores seeded in axenic culture is described for several races of Puccinia graminis Pers. f. sp. tritici (Erikss. and Henn.) and race 3 of Melampsora lini (Ehrenb.) Lév. on complex media containing peptone, yeast extract, and bovine serum albumin (BSA); and for an Australian isolate of Puccinia graminis, race 126-ANZ 6,7, and Melampsora lini, race 3, on chemically defined, liquid media.Of six North American isolates of Puccinia graminis only race 38 formed colonies approaching those of race 126-ANZ 6,7 in final size and general morphology on complex media. 5′AMP had no effect on the growth of 126-ANZ 6,7, but cyclic AMP inhibited growth after uredospore germination. Good growth and sporulation were obtained with 126-ANZ 6,7, but not with the other isolates tested, using a new, chemically defined liquid medium, sterilized by millipore filtration, and containing glucose, Czapek's minerals plus micronutrients, Ca2+, glucose and aspartic acid, glutathione, and cysteine. Uredospores produced in culture reinfected exposed mesophyll tissue, but not intact seedling leaves of wheat.Highly reproducible growth and sporulation of Melampsora lini, race 3, were obtained routinely on a solid medium containing Difco-Bacto agar, sucrose, Knop's minerals, micronutrients, yeast extract, peptone, and BSA. Vegetative cultures, capable of reinfecting the cut ends of surface-sterilized flax cotyledons, could be maintained indefinitely by subdivision before sporulation and transfer to the same medium minus BSA. Evidence is presented that BSA stimulated the development of colonies and the formation of uredospores. The mode of action of BSA is unknown, but it could not be replaced by putrescine.A new chemically defined, liquid medium containing sucrose, Knop's mineral salts, micronutrients, aspartic (or glutamic) acid, and cysteine supported the growth of colonies of Melampsora lini in a highly reproducible manner. The formation of uredospores and teliospores by these colonies was controlled by (a) the level of Ca2+ (as Ca(NO3)2∙4H2O), (b) the concentration of aspartic acid, and (c) the number of colonies per flask. At inoculum levels giving 40 to 60 colonies per flask, in media containing 8.5 mM Ca+ and 45 mM aspartic acid, uredospore formation occurred in 60 to 70% of the colonies. A decrease in the Ca2+ level to 4.25 mM, or a decrease in aspartic acid to 22.5 mM, or adjustment of the inoculum level to give about 10 colonies per flask each resulted in only infrequent sporulation. The uredospores produced in vitro infected intact, 1-week-old flax cotyledons in a normal manner.

MICROBIAL PENTOSANASES: II. SOME FACTORS AFFECTING THE PRODUCTION OF PENTOSANASES BY BACILLUS PUMILUS AND BACILLUS SUBTILIS

1956 ◽  
Vol 2 (1) ◽  
pp. 28-38 ◽  
Author(s):  
F. J. Simpson
Keyword(s):  
Yeast Extract ◽  
Wheat Bran ◽  
Soybean Meal ◽  
Corn Steep Liquor ◽  
Ammonium Phosphate ◽  
Water Soluble ◽  
High Rate ◽  
Mineral Salts ◽  
Ammonium Lactate ◽  
Steep Liquor

A number of carbohydrates and nitrogenous adjuncts were tested for their effect on the constitutive and adaptive pentosanases produced by Bacillus stibtilis and B. pumilus respectively in a medium containing biotin, ammonium phosphate, and other mineral salts. B. subtilis produced more enzyme with sulphite liquor than with any of the other carbohydrate sources tested. Next, in decreasing order of merit, were wheat bran, maltose, ribose, beet molasses, oat hulls, and pectin. Of the nitrogenous adjuncts tested, corn steep liquor, soybean meal, gelatin, gelysate, and ammonium lactate doubled the yield of enzyme whereas yeast extract, peptone, urea, and others were less effective. For B. pumilus the better carbohydrate sources, in decreasing order of merit, were wheat bran, water soluble pentosan of wheat flour, xylan, straw holo-cellulose, wheat straw, and sulphite liquor. Of the nitrogen sources, corn steep liquor was outstanding while casein, casitone, phytone, yeast extract, distillers' dried solubles, and soybean meal followed in decreasing order. A medium containing 6% wheat bran (20 mesh), 1% corn steep liquor neutralized with ammonia, 0.05% sodium chloride, and 0.05% calcium carbonate was devised for the production of pentosanase by B. pumilus. With this medium in shaken Erlenmeyer flasks, the enzyme was produced at a high rate between 12 and 40 hr.; thereafter the rate of production decreased. Maximum yields were obtained in 96 hr. A temperature of 26 °C. was more favorable for pentosanase production than higher temperatures.

Sign in / Sign up

Export Citation Format

Share Document