PREPARATION AND EVALUATION OF POLYSTYRENE–ANTIGEN CONJUGATES FOR THE ISOLATION OF ANTIBODIES

1960 ◽  
Vol 38 (11) ◽  
pp. 1235-1248 ◽  
Author(s):  
L. Gyenes ◽  
A. H. Sehon
Keyword(s):  
Hydrochloric Acid ◽  
Soluble Protein ◽  
Radioactive Tracer ◽  
Optimal Conditions ◽  
Protein Antigens ◽  
Tracer Methods ◽  
Antigen Antibody ◽  
Preparation And Evaluation

Antigenically specific adsorbents were prepared by coupling soluble protein antigens to a polystyrene-supporting medium by azo bonds. These immunosorbents were shown to combine specifically with homologous precipitating rabbit antibodies. Elution of precipitating antibodies from the immunosorbents was achieved by dissociation with hydrochloric acid at pH 3. Under optimal conditions 35% of the antibodies removed from the antisera were recovered with a purity of over 80%. Electrophoretic analyses showed that precipitating rabbit antibodies were localized primarily in the region of gamma-globulins. The heterogeneity of the antigen–antibody systems studied was demonstrated by a combination of immunochemical, physicochemical, and radioactive tracer methods.

PREPARATION AND EVALUATION OF POLYSTYRENE–ANTIGEN CONJUGATES FOR THE ISOLATION OF ANTIBODIES

1960 ◽  
Vol 38 (1) ◽  
pp. 1235-1248 ◽  
Author(s):  
L. Gyenes ◽  
A. H. Sehon
Keyword(s):  
Hydrochloric Acid ◽  
Soluble Protein ◽  
Radioactive Tracer ◽  
Optimal Conditions ◽  
Protein Antigens ◽  
Tracer Methods ◽  
Antigen Antibody ◽  
Preparation And Evaluation

Antigenically specific adsorbents were prepared by coupling soluble protein antigens to a polystyrene-supporting medium by azo bonds. These immunosorbents were shown to combine specifically with homologous precipitating rabbit antibodies. Elution of precipitating antibodies from the immunosorbents was achieved by dissociation with hydrochloric acid at pH 3. Under optimal conditions 35% of the antibodies removed from the antisera were recovered with a purity of over 80%. Electrophoretic analyses showed that precipitating rabbit antibodies were localized primarily in the region of gamma-globulins. The heterogeneity of the antigen–antibody systems studied was demonstrated by a combination of immunochemical, physicochemical, and radioactive tracer methods.

Experimental Study on Concentrating Scandium by Leaching from Associated Scandium Ore

2013 ◽  
Vol 734-737 ◽  
pp. 1033-1036
Author(s):  
Gui Fang Zhang ◽  
Peng Yan ◽  
Qing Rong Yang
Keyword(s):  
Experimental Study ◽  
Particle Size ◽  
Hydrochloric Acid ◽  
Acid Concentration ◽  
Hydrochloric Acid Concentration ◽  
Leaching Rate ◽  
Optimal Conditions ◽  
Research Results ◽  
Solid Ratio ◽  
Leaching Residue

Based on the benefication of the complex silicate ore containing scandium, the research about aid-leaching agent used in the leaching of the scandium concentrate was been conducted. And the suitable leaching agent and aid-leaching agent which the useful ions entered into leaching liquid and the harmful ions were kept in leaching residue were been found according to the experiment results. For the scandium of sample existed various complex silicate ore as isomorphism form, the research has adopted hydrochloric acid with aid-leaching agent to dissociate the silicate ore and make scandium entering into solution. The research results has shown that the scandium leaching rate could reach 92.06% under the optimal conditions which the hydrochloric acid concentration is 22.8%, the dosage of aid leaching agent is 6%, liquid solid ratio is 4:1, particle size of leaching material totally is less than 0.15mm and leaching time is 8h.

Paper 12: Hydrodynamic Theory of Dynamically Loaded Bearings and its Application to Engine Bearing Design

1966 ◽  
Vol 181 (2) ◽  
pp. 35-44
Author(s):  
H. W. Hahn ◽  
K. Radermacher
Keyword(s):  
Early Stage ◽  
Radioactive Tracer ◽  
Hydrodynamic Theory ◽  
Design Stage ◽  
Fast Running ◽  
Engine Design ◽  
Load Carrying ◽  
Dynamically Loaded ◽  
Tracer Methods ◽  
Engine Bearing

In recent years the hydrodynamic theory of dynamically loaded engine bearings has been developed to such an extent that calculations of bearing behaviour under all kinds of loads can be made with good correlation to experimental results and field experience. In addition to these calculations, experiments have been performed to determine the influence of variables in practical bearings such as oil-inlet pressure or temperature, the shape of the oil inlet, and the quantity of the lubricant. At the engine-design stage, engine-bearing behaviour can be predicted by using calculations based on hydrodynamic theory. Some examples are given to show that the load-carrying capacity and consequently the reliability of engine bearings can be increased by using these calculation methods on a digital computer and with a variation of bearing characteristics, such as dimensions, clearance, oil pressure, temperature, and viscosity, or with a variation of load characteristics such as magnitude or direction of counterweight-mass forces, gas pressure, and firing order. Very good correlation is obtained between these predictions and real engine-bearing behaviour in fast-running diesel engines, as measured by radioactive tracer methods. Many German diesel-engine producers are using these calculations in an early stage of engine design.

STUDIES ON THE EFFECT OF CORTISOL AND CORTICOTROPHIN ON INCORPORATION OF ADENINE-8-14C INTO THE LYMPHATIC TISSUE NUCLEIC ACIDS

1965 ◽  
Vol 49 (3) ◽  
pp. 471-478 ◽  
Author(s):  
Truls Brinck-Johnsen ◽  
Thomas F. Dougherly
Keyword(s):  
Nucleic Acids ◽  
Lymph Nodes ◽  
Quantitative Measurement ◽  
Radioactive Tracer ◽  
Prolonged Treatment ◽  
Lymphatic Tissue ◽  
Tracer Methods ◽  
Rna And Dna

ABSTRACT The effects of cortisol and ACTH in vivo on various mouse lymphatic tissues were studied, using the current techniques for isolation of adenine from nucleic acids and quantitative measurement, including chromatography, spectrophotometry and radioactive tracer methods. The incorporation of adenine-8-14C into both RNA and DNA of lymphatic tissues of intact mice was reduced following prolonged treatment with cortisol or ACTH. In the absence of the adrenals ACTH enhanced the incorporation of adenine-8-14C into the RNA of lymph nodes, spleen and thymus, although a clearcut effect on increased incorporation of the purine into DNA was limited to the spleen. It was concluded that ACTH has an extraadrenal effect on lymphatic tissues.

In vitro primary responses of human T cells to soluble protein antigens

1994 ◽  
Vol 170 (1) ◽  
pp. 15-25 ◽  
Author(s):  
Magdalena Plebanski ◽  
Sally S. Burtles
Keyword(s):  
T Cells ◽  
Soluble Protein ◽  
Protein Antigens ◽  
Human T Cells
Sign in / Sign up

Export Citation Format

Share Document