STUDIES ON WHEAT PLANTS USING CARBON-14 COMPOUNDS: XXI. THE METABOLISM OF GLYCINE-2-C14

1964 ◽  
Vol 42 (9) ◽  
pp. 1293-1299 ◽  
Author(s):  
W. B. McConnell
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Glutamic Acid ◽  
Specific Activity ◽  
Total Carbon ◽  
Gluten Protein ◽  
Carbon 14 ◽  
Specific Activities

Glycine-2-C14was administered to 83-day-old wheat plants. The plants were allowed to mature fully and the carbon-14 distribution was then examined. About 80% of the radioactivity injected was recovered in the upper portions of the plant, the kernels themselves containing 66%. Proteins had a higher specific activity than other kernel constituents but the starch contained about one-half the total carbon-14 of the kernels. Glycine and serine were by far the most radioactive amino acids of the gluten protein. They had specific activities of 2720 and 2900 μc/mole C respectively while alanine, histidine, methionine, glutamic acid, and proline had specific activities ranging from 150 to 300 μc/mole C. The specific activities of carbons 1 and 2 of glycine recovered from the protein were 550 and 4900 μc/mole respectively while the specific activities of carbons 1, 2, and 3 of serine were 490, 4300, and 3100 μc/mole respectively. The results confirm previous views regarding extensive interconversion of glycine and serine in maturing wheat. Extensive labelling in carbon 3 of serine is interpreted as evidence that glycine is degraded to "active formaldehyde" and carbon dioxide.

STUDIES ON WHEAT PLANTS USING C14 COMPOUNDS: VII. UTILIZATION OF PYRUVATE-2-C14

1958 ◽  
Vol 36 (1) ◽  
pp. 381-388 ◽  
Author(s):  
E. Bilinski ◽  
W. B. McConnell
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Active Carbon ◽  
Specific Activity ◽  
Carboxyl Groups ◽  
Gluten Protein ◽  
Carbon 14 ◽  
Soluble Material

Approximately half of the carbon-14 injected into the stems of wheat plants in the form of pyruvate-2-C14 remained in the plant at maturity, 30 days later. Almost 90% of this had accumulated in the kernel. Appreciable activity was found in the major components, protein, starch, ether-soluble material, and a residue termed bran. The amino acids of the gluten protein differed markedly from one another in specific activity. Glutamic acid and the related amino acids, arginine and proline, were most active, their specific activity decreasing in that order. Fifty-two per cent of the carbon-14 in glutamic acid was in carbon-5, while carbon-1 contained 21%. Seventy per cent of the radioactivity of aspartic acid was divided almost equally between the terminal carboxyl groups. The results are similar to those previously observed using acetate-1-C14 as tracer, and it is concluded that administered pyruvate-2-C14 undergoes extensive decarboxylation to form acetate-1-C14. The most active carbon in alanine from the pyruvate-2-C14 was carbon-1. This observation is not in accord with the theory that alanine is formed directly from pyruvate by transamination.

STUDIES ON WHEAT PLANTS USING C14 COMPOUNDS: VII. UTILIZATION OF PYRUVATE-2-C14

1958 ◽  
Vol 36 (4) ◽  
pp. 381-388 ◽  
Author(s):  
E. Bilinski ◽  
W. B. McConnell
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Aspartic Acid ◽  
Active Carbon ◽  
Specific Activity ◽  
Carboxyl Groups ◽  
Gluten Protein ◽  
Carbon 14 ◽  
Soluble Material

Approximately half of the carbon-14 injected into the stems of wheat plants in the form of pyruvate-2-C14 remained in the plant at maturity, 30 days later. Almost 90% of this had accumulated in the kernel. Appreciable activity was found in the major components, protein, starch, ether-soluble material, and a residue termed bran. The amino acids of the gluten protein differed markedly from one another in specific activity. Glutamic acid and the related amino acids, arginine and proline, were most active, their specific activity decreasing in that order. Fifty-two per cent of the carbon-14 in glutamic acid was in carbon-5, while carbon-1 contained 21%. Seventy per cent of the radioactivity of aspartic acid was divided almost equally between the terminal carboxyl groups. The results are similar to those previously observed using acetate-1-C14 as tracer, and it is concluded that administered pyruvate-2-C14 undergoes extensive decarboxylation to form acetate-1-C14. The most active carbon in alanine from the pyruvate-2-C14 was carbon-1. This observation is not in accord with the theory that alanine is formed directly from pyruvate by transamination.

THE METABOLISM OF VALERATE-2-C14 BY UREDOSPORES OF WHEAT STEM RUST

1963 ◽  
Vol 41 (1) ◽  
pp. 1-7 ◽  
Author(s):  
H. Reisener ◽  
A. J. Finlayson ◽  
W. B. McConnell
Keyword(s):  
Carbon Dioxide ◽  
Glutamic Acid ◽  
Specific Activity ◽  
Tricarboxylic Acid Cycle ◽  
High Specific Activity ◽  
Water Soluble ◽  
Buffer Solution ◽  
Wheat Stem Rust ◽  
Carbon 14 ◽  
Water Soluble Extract

When uredospores of Puccinia graminis var. tritici race 15B were shaken in a medium containing M/30 phosphate buffer, pH 6.2, and valerate-2-C14, about 88% of the radioactivity was removed from the buffer solution in a period of 3 hours. About 40% of the carbon-14 taken from the buffer was found in a water-soluble extract of the spores and about 15% was respired as carbon dioxide. The result is compared with an earlier report that carbon 1 of valerate is more extensively released as carbon dioxide and less extensively incorporated into spore components. Glutamic acid, glutamine, γ-aminobutyric acid, and alanine of high specific activity were isolated. It was estimated from partial degradation that more than one-half of the carbon-14 of glutamic acid occurred in position 4 and that carbon 5 was very weakly labelled. Citric acid was also of high specific activity and was labelled predominantly in the internal carbons.It is concluded that respiring rust spores utilize externally supplied valerate by β-oxidation, which releases carbons 1 and 2 in a form which is metabolized as acetate by the tricarboxylic acid cycle.

STUDIES ON WHEAT PLANTS USING CARBON-14 LABELLED COMPOUNDS: X. THE INCORPORATION OF GLUTAMIC ACID-l-C14

1959 ◽  
Vol 37 (1) ◽  
pp. 933-936 ◽  
Author(s):  
W. B. McConnell
Keyword(s):  
Amino Acids ◽  
Glutamic Acid ◽  
Specific Activity ◽  
Wheat Plant ◽  
Protein Fractions ◽  
Appreciable Amount ◽  
Kernel Development ◽  
Carbon 14

Glutamic acid-1-C14 was injected into the top internode of wheat stems at a stage of growth when kernel development was rapid (71 days after seeding). The plants were harvested 31 days later when they had matured and the incorporation of carbon-14 studied. About one-third of the carbon-14 administered was found in the upper portions of the mature plants, much of the remaining radioactivity having apparently been respired. About 85% of the carbon-14 recovered was found in the kernel. The protein fractions of these were most radioactive, but an appreciable amount of carbon-14 also appeared in the starch. Glutamic acid had the highest specific activity of the amino acids isolated from the gluten, but proline and arginine were also strongly labelled. Since these three amino acids were labelled predominantly in carbon-1 their close metabolic relationship in the wheat plant seems probable.

THE METABOLISM OF PROPIONATE BY WHEAT STEM RUST UREDOSPORES

1963 ◽  
Vol 41 (3) ◽  
pp. 737-743 ◽  
Author(s):  
H. Reisener ◽  
A. J. Finlayson ◽  
W. B. McConnell ◽  
G. A. Ledingham
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Glutamic Acid ◽  
Stem Rust ◽  
Water Extract ◽  
Hot Water ◽  
Insoluble Residue ◽  
Soluble Carbohydrates ◽  
Wheat Stem Rust ◽  
Carbon 14

When uredospores of wheat stem rust were shaken for 3 hours with phosphate buffer (pH 6.2) containing propionate-1-C14, -2-C14, or -3-C14, about 55% of the carbon-14 was removed from the solution. With propionate-1-C14, most of the carbon-14 taken up was released as carbon dioxide-C14, whereas about 20% and 31% of propionate carbon 2 and carbon 3, respectively, was incorporated into the spores. The specific activity of a fraction consisting of the free amino acids of a hot-alcohol and hot-water extract of the spores increased markedly with increase in the position number of propionate in which the carbon-14 was located. A similar relation was observed for other fractions such as soluble carbohydrates, ether-soluble material, organic acids, and insoluble residue from spores. The most active amino acids isolated were glutamic acid, γ-aminobutyric acid, and alanine. Partial degradations showed that with propionate-2-C14 the carboxyl groups of glutamic acid were especially radioactive, whereas with propionate-3-C14 the internal carbons were most radioactive.It is concluded that propionate metabolism in the rust spores involved conversion of carbon 1 to carbon dioxide, and utilization of carbons 2 and 3 as acetate with carbon 2 behaving as the carboxyl carbon.

PYOCYANINE FORMATION FROM LABELLED SUBSTRATES BY PSEUDOMONAS AERUGINOSA

1957 ◽  
Vol 3 (2) ◽  
pp. 165-169 ◽  
Author(s):  
A. C. Blackwood ◽  
A. C. Neish
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Pseudomonas Aeruginosa ◽  
Calcium Carbonate ◽  
Specific Activity ◽  
The Other ◽  
Specific Activities ◽  
Carbonate Salts ◽  
Cell Carbon

Pseudomonas aeruginosa was grown under conditions suitable for pyocyanine production in a medium containing glycerol, L-leucine, DL-alanine, calcium carbonate, salts, and small amounts of various C14-labelled substrates. A comparison of the specific activities of the cell carbon, respiratory carbon dioxide, and pyocyanine carbon showed that glycerol and dihydroxyacetone were the only substrates from which pyocyanine having a specific activity higher than the cell carbon was formed. Glucose, fructose, pyruvate, acetate, and the 13 amino acids tested were inferior in this respect. Alanine, leucine, isoleucine, and glycine were incorporated into pyocyanine more readily than the other amino acids. Phenylalanine and tyrosine, although possessing preformed rings, were poor precursors of pyocyanine and were oxidized more readily than they were assimilated. These results suggest that pyocyanine originates from trioses but gives little indication of the nature of the intermediates.

scholarly journals Incorporation of dl-[2−14C]ornithine and dl-[5−14C]arginine in milk constituents by the isolated lactating sheep udder

1968 ◽  
Vol 106 (3) ◽  
pp. 719-724 ◽  
Author(s):  
R. Verbeke ◽  
G. Peeters ◽  
Anne Marie Massart-Leën ◽  
G. Cocquyt
Keyword(s):  
Carbon Dioxide ◽  
Amino Acids ◽  
Glutamic Acid ◽  
Mammary Glands ◽  
Total Radioactivity ◽  
Chemical Degradation ◽  
Mammary Tissue ◽  
Specific Activities ◽  
Plasma Arginine

1. Lactating mammary glands of sheep were perfused for several hours in the presence of dl-[2−14C]ornithine or dl-[5−14C]arginine and received adequate quantities of acetate, glucose and amino acids. 2. In the [14C]ornithine experiment 1·4% of the casein and 1% of the expired carbon dioxide came from added ornithine; 96% of the total radioactivity in casein was recovered in proline; 13% of the proline of casein originated from plasma ornithine. 3. In this experiment the results of chemical degradation of proline of casein as well as relative specific activities in the isolated products are consistent with the view that ornithine is metabolized, by way of glutamic γ-semialdehyde, to proline or glutamic acid. 4. In the [14C]arginine experiments 3% of the casein and 1% of the expired carbon dioxide came from arginine; 84% of the arginine and 9% of the proline of casein originated from plasma arginine. 5. In these experiments the relative specific activities of arginine, ornithine and proline in plasma are in agreement with the view that arginine is metabolized by way of ornithine to proline. The conversion of arginine into ornithine is probably catalysed by arginase, so that arginase in mammary tissue may be involved in the process of milk synthesis.

STUDIES ON WHEAT PLANTS USING CARBON-14 COMPOUNDS: XI THE RELATIVE INCORPORATION OF CARBONS 1, 2, AND 3 OF PYRUVATE

1960 ◽  
Vol 38 (1) ◽  
pp. 33-41 ◽  
Author(s):  
W. B. McConnell ◽  
R. Nath ◽  
J. F. T. Spencer
Keyword(s):  
Carbon Dioxide ◽  
Glutamic Acid ◽  
Specific Activity ◽  
Carbon Skeleton ◽  
Carbon 14

The incorporation of carbons 1, 2, and 3 of pyruvate into maturing plants has been compared by use of pyruvate-1-C14, -2-C14, and -3-C14 as tracers. The carbon-14 content of kernel proteins and lipids increased markedly when pyruvate was labelled in higher-number positions. The effect was strikingly demonstrated in the glutamic acid from the protein in which the specific activity varied in the proportion 1:5:10.5 when pyruvate labelled in the 1, 2, or 3 position respectively was administered. A small amount of administered pyruvate appeared to be utilized as the intact carbon skeleton for biosynthesis of starch and of alanine. It is considered, however, that most of the injected pyruvate undergoes decarboxylation, carbon-1 being utilized as carbon dioxide and carbons 2 and 3 as acetate.

STUDIES ON WHEAT PLANTS WITH CARBON-14 COMPOUNDS: XX. THE METABOLISM OF PROPIONIC ACID

1964 ◽  
Vol 42 (2) ◽  
pp. 187-193 ◽  
Author(s):  
W. B. McConnell ◽  
A. J. Finlayson
Keyword(s):  
Carbon Dioxide ◽  
Glutamic Acid ◽  
Propionic Acid ◽  
Specific Activity ◽  
High Specific Activity ◽  
Similar Data ◽  
Tissue Slices ◽  
Wheat Seedlings ◽  
Carbon 14 ◽  
Kernel Protein

The metabolism of propionic acid by maturing wheat plants was investigated by use of the radioactive tracers propionate-1-C14, -2-C14, and -3-C14. Carbon 2 of propionate was most extensively incorporated into kernel components and yielded kernel protein of high specific activity, glutamic acid being particularly radioactive. Carbon 3 was also preferentially incorporated into glutamic acid but was not as efficient in this regard as was carbon 2. Carbon 1 of propionate was extensively respired as carbon dioxide. It did not label glutamic acid extensively. Partial degradation of glutamic acid from kernel protein hydrolyzates showed that carbon 1 of propionate labelled carbon 1 of glutamate more than it did other glutamate carbons. Carbon 2 of propionate preferentially labelled carbon 4 of glutamate and carbon 3 preferentially labelled carbon 5 of glutamate. Similar data were obtained by examining the carbon-14 distribution in free glutamic acid obtained from wheat seedlings labelled with radioactive propionate-1-C14, -2-C14, and -3-C14.The results are interpreted as evidence that propionate is degraded by conversion of carbon 1 to carbon dioxide and by utilization of carbons 2 and 3 as acetate, with carbon 3 behaving as the carboxyl carbon of acetate. They accord with views on the mode of propionate metabolism derived from studies with plant tissue slices.

STUDIES ON WHEAT PLANTS USING CARBON-14 COMPOUNDS: XI THE RELATIVE INCORPORATION OF CARBONS 1, 2, AND 3 OF PYRUVATE

1960 ◽  
Vol 38 (1) ◽  
pp. 33-41
Author(s):  
W. B. McConnell ◽  
R. Nath ◽  
J. F. T. Spencer
Keyword(s):  
Carbon Dioxide ◽  
Glutamic Acid ◽  
Specific Activity ◽  
Carbon Skeleton ◽  
Carbon 14

The incorporation of carbons 1, 2, and 3 of pyruvate into maturing plants has been compared by use of pyruvate-1-C14, -2-C14, and -3-C14 as tracers. The carbon-14 content of kernel proteins and lipids increased markedly when pyruvate was labelled in higher-number positions. The effect was strikingly demonstrated in the glutamic acid from the protein in which the specific activity varied in the proportion 1:5:10.5 when pyruvate labelled in the 1, 2, or 3 position respectively was administered. A small amount of administered pyruvate appeared to be utilized as the intact carbon skeleton for biosynthesis of starch and of alanine. It is considered, however, that most of the injected pyruvate undergoes decarboxylation, carbon-1 being utilized as carbon dioxide and carbons 2 and 3 as acetate.

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