FRUCTOSE DIPHOSPHATE ALDOLASE FROM FUSARIUM OXYSPORUM f. LYGOPERSICI: II. CHARACTERISTICS OF THE EXCHANGE REACTION
Fruetose-1,6-diphosphate aldolase purified from extracts of Fusarium oxysporum f. lycopersici was examined in detail. The different pH optima obtained for the proton exchange (pH 6.0) and cleavage (pH 7.5) reactions indicate that this enzyme resembles the yeast FDP-aldolase rather than the aldolase from muscle. When the enzyme was dialyzed extensively, in the absence of Cleland's reagent, it was found that neither the cleavage nor the exchange reaction rates were increased appreciably after addition of mercaptan. Treatment of the enzyme with either methylene blue in the presence of light or with diazosulfanilic acid, two known inhibitors of the cleavage reaction, resulted in almost complete inhibition of FDP cleavage but only a twofold decrease in the exchange capacity. These results substantiate our previous findings that the inhibitors react with residues involved in the D-glyceraldehyde-3-phosphate rather than the dihydroxyacetone phosphate active site.