Effect of growth hormone and hydrocortisone on the synthesis of rapidly labeled, high molecular weight, nuclear RNA in rat liver

1969 ◽  
Vol 47 (2) ◽  
pp. 226-229 ◽  
Author(s):  
Maurice Brossard ◽  
Louis Nicole
Keyword(s):  
Molecular Weight ◽  
Growth Hormone ◽  
High Molecular Weight ◽  
Rat Liver ◽  
Orotic Acid ◽  
The Other ◽  
Other Hand ◽  
Nuclear Rna ◽  
Nucleolar Rna

The effects of growth hormone and hydrocortisone on the synthesis of rat liver RNA have been studied. Administration of growth hormone stimulated the incorporation of 14C-orotic acid into 45 S preribosomal nucleolar RNA, and had no effect on the 6–50 S polydisperse nuclear RNA of extra-nucleolar origin. On the other hand, hydrocortisone stimulated the synthesis of all types of nuclear RNA, but mainly that of the 18 S class of 6–50 S polydisperse nuclear RNA.

Metabolism of high molecular weight, polydisperse, rapidly labeled nuclear RNA in rat liver

1968 ◽  
Vol 46 (12) ◽  
pp. 1497-1505 ◽  
Author(s):  
Maurice Brossard ◽  
Louis Nicole
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Rat Liver ◽  
Ribosomal Rna ◽  
Base Composition ◽  
Orotic Acid ◽  
Chromosomal Origin ◽  
Base Analysis ◽  
Nuclear Rna ◽  
Kinetics Of

Studies of the metabolism of rat liver RNA showed the existence of two species of rapidly labeled nuclear RNA: a 45 S preribosomal type of nucleolar origin, and a 6–50 S polydisperse RNA of chromosomal origin. The kinetics of labeling with orotic acid-14C and the nature of the latter RNA have been investigated. The following findings are reported, (1) This RNA is composed of at least four main classes of RNA having sedimentation coefficients of approximately 45, 35, 24, and 18 S. (2) Except for the 18 S class which seems to be an end product, the three other classes have a rapid turnover and do not appear to leave the nucleus. (3) Base analysis after 32P incorporation indicates that these four classes of RNA have a similar base composition with a G+C/A + U ratio in the range of 0.98–1.07, which resembles DNA more closely than ribosomal RNA. (4) The 6–50 S polydisperse RNA has a different metabolism than that of the 45 S preribosomal type and there is no precursor-to-product relationship between these two species of RNA.

Differential Effects of Growth Hormone and Hydrocortisone on Nuclear RNA Synthesis in Rat Liver

1971 ◽  
Vol 49 (7) ◽  
pp. 853-862 ◽  
Author(s):  
Peter M. K. Ip ◽  
Maurice Brossard
Keyword(s):  
Growth Hormone ◽  
Rat Liver ◽  
Rna Synthesis ◽  
Present Report ◽  
Liver Nucleus ◽  
Double Labeling ◽  
Nuclear Rna Synthesis ◽  
Nuclear Rna ◽  
Liver Nuclei ◽  
Nucleolar Rna

RNA has been isolated and fractionated from rat liver nuclei by a sequence of salt extractions into four subfractions: namely nucleoplasmic, deoxyribonucleoprotein-associated, ribonucleoprotein-associated, and nucleolar fractions.Study of 14C-methyl-methionine labeling indicates that the nucleolar RNA fraction isolated by the present procedure is, in fact, of nucleolar origin while the other three fractions are essentially of extranucleolar origin.The effects of growth hormone and hydrocortisone on the nuclear RNA synthesis have been further studied using the present isolation and double-labeling techniques. Both hormones cause an increase in the incorporation of labeled orotic acid into all types of RNA in all four nuclear subfractions. However, the stimulatory effect of growth hormone is found mainly in the 18–28 S and 45–60 S regions of the nucleolar fraction and in regions of the entire gradient of the nucleoplasmic fraction, whereas the stimulatory effect of hydrocortisone is localized mainly in the 10–28 S regions of the nucleolar fraction and in the 4 S and 10–18 S regions of the ribonucleoprotein fraction.The present report suggests that there are qualitative as well as quantitative differences in the action of growth hormone and hydrocortisone on the synthesis of RNA in liver nucleus. The mode of action of the two hormones is discussed.

Rapid Isolation of High Molecular Weight Urokinase from Native Human Urine

1982 ◽  
Vol 47 (03) ◽  
pp. 197-202 ◽  
Author(s):  
Kurt Huber ◽  
Johannes Kirchheimer ◽  
Bernd R Binder
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Human Urine ◽  
Gel Filtration ◽  
Chain Structure ◽  
The Other ◽  
Single Chain ◽  
Apparent Homogeneity ◽  
Sequential Adsorption

SummaryUrokinase (UK) could be purified to apparent homogeneity starting from crude urine by sequential adsorption and elution of the enzyme to gelatine-Sepharose and agmatine-Sepharose followed by gel filtration on Sephadex G-150. The purified product exhibited characteristics of the high molecular weight urokinase (HMW-UK) but did contain two distinct entities, one of which exhibited a two chain structure as reported for the HMW-UK while the other one exhibited an apparent single chain structure. The purification described is rapid and simple and results in an enzyme with probably no major alterations. Yields are high enough to obtain purified enzymes for characterization of UK from individual donors.

Barricyclin D1—a dimeric ellagitannin with a macrocyclic structure—and accompanying tannins from Barringtonia racemosa

2021 ◽  
Author(s):  
Shinji Yoshikawa ◽  
Lih-Geeng Chen ◽  
Morio Yoshimura ◽  
Yoshiaki Amakura ◽  
Tsutomu Hatano ◽  
...  
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Natural Resource ◽  
The Other ◽  
Hydrolysable Tannin ◽  
The Family ◽  
Macrocyclic Structure

Abstract Our examination of high molecular weight polyphenolic constituents in the leaves of Barringtonia racemosa of the family Lecythidaceae uncovered five previously undescribed ellagitannins. One, barringtin M1 (1), among them was a hydrolysable tannin monomer, while remaining four, barringtins D1 (2), D2 (3), D3 (4) and barricyclin D1 (5), were all dimers. Barricyclin D1 had a first macrocyclic structure formed from casuarictin (6) and tellimagrandin I (7), and the other ellagitannins had structures related to 5. Two additional known phenolics, valoneic acid dilactone (8) and schimawalin A (9), were also isolated from the leaves. These results suggested that the leaves of B. racemosa is a natural resource rich in hydrolysable tannin oligomers.

U1 small nuclear RNA-like sequences in human high molecular weight RNA

1984 ◽  
Vol 122 (2) ◽  
pp. 838-844 ◽  
Author(s):  
J.M. Capasso ◽  
M.A. Docherty ◽  
A. Ray ◽  
E.D. Kaplan ◽  
G.L. Eliceiri
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Small Nuclear Rna ◽  
Nuclear Rna

Effect on Polymer Chain Structure Caused by the Molar Fraction of 4-Hydroxybutyrate in Poly(3-hydroxybutyrate- co -4-hydroxybutyrate)

2012 ◽  
Vol 602-604 ◽  
pp. 776-780
Author(s):  
Zhi Qiang Li ◽  
Mei Li ◽  
Wei Jia Fan
Keyword(s):  
Molecular Weight ◽  
Intrinsic Viscosity ◽  
Polymer Chain ◽  
Molar Fraction ◽  
Chain Structure ◽  
The Other ◽  
Mean Square ◽  
Polarizing Microscope ◽  
Other Hand ◽  
The Mean

Poly(3-hydroxybutyrate-co-4-hydroxybutyrate)copolymer [P(3HB-co-4HB)] is a kind of biodegradable high molecular polymer produced by bioaccumulation. Because of the good biodegradability and biocompatibility, P(3HB-co-4HB)s have attracted wide attention . At first, the intrinsic viscosity[η] in good solvent of P(3HB-co-4HB) s with varying contents of 4HB was investigated in different temperature. Second, observed the changes of crystallization gathered state caused by the varying contents of 4HB by polarizing microscope. The results show that to the P(3HB-co-4HB)s in same molecular weight, the intrinsic viscosity[η] in good solvent barely changes when the mole fractions of 4HB increase. On the other hand, the mean square end to end distances[0] of macromolecular flexible chains increase with the mole fractions of 4HB. At the same time, the states of aggregation change from spherulites to dendrites. In this investigation, we discuss the reasons of the differences in depth.

Isolation of a polydisperse high-molecular-weight glycogen from rat liver

1981 ◽  
Vol 111 (1) ◽  
pp. 137-145 ◽  
Author(s):  
Doris M. Haverstick ◽  
Alvin H. Gold
Keyword(s):  
Molecular Weight ◽  
High Molecular Weight ◽  
Rat Liver

scholarly journals ELECTROPHORETIC STUDIES ON PR8 INFLUENZA VIRUS

1944 ◽  
Vol 80 (6) ◽  
pp. 549-559 ◽  
Author(s):  
Gail Lorenz Miller ◽  
Max A. Lauffer ◽  
W. M. Stanley
Keyword(s):  
Molecular Weight ◽  
Influenza Virus ◽  
High Molecular Weight ◽  
Isoelectric Point ◽  
High Speed ◽  
The Other ◽  
Active Virus ◽  
Electrophoresis Method

Crude preparations of PR8 influenza virus, obtained by high-speed centrifugation, contain two electrophoretically distinct components. One of these, present to the extent of 10 to 20 per cent, was identified by electrophoresis, centrifuge, and activity tests, as an impurity similar to or identical with a high molecular weight acidic substance shown by Knight to be elaborated by normal uninfected embryos. The other component, present to the extent of 80 to 90 per cent, appeared to represent the active virus. The virus fraction was separated from the impurity by repeated fractional centrifugation. It then appeared homogeneous in the analytical centrifuge and in the Tiselius apparatus, and possessed an isoelectric point at pH 5.3 as measured by the micro-electrophoresis method.

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