Ultraviolet circular dichroism studies on synthetic polynucleotides
The ultraviolet circular dichroism spectra of polyadenylic acid, polyguanylic acid, polycytidylic acid, polyinosinic acid, and polyuridylic acid have been examined at neutral and acidic pH values, and at moderate and low ionic strengths, over the wavelength range 300–185 mμ. Increased resolution of spectra below 225 mμ has revealed heretofore unexamined ellipticity bands in the low wavelength region, which are sensitive to conformational alterations for those polynucleotides which exhibit both single and multistranded secondary structures. It is concluded that these ellipticity bands, in view of their extreme sensitivity to conformation, will be of significance in increasing the usefulness of the homopolynucleotides as model compounds in conformational studies of naturally occurring RNAs.