Physiological and genetic characterization of fluorescentPseudomonasassociated withCantharellus cibarius

2002 ◽  
Vol 48 (8) ◽  
pp. 739-748 ◽  
Author(s):  
J Ignacio Rangel-Castro ◽  
Jolanta J Levenfors ◽  
Eric Danell
Keyword(s):  
16S Rdna ◽  
Fungal Mycelium ◽  
Sequencing Analysis ◽  
Carbon Utilization ◽  
Pseudomonas Spp ◽  
Fluorescent Pseudomonas ◽  
Rdna Sequencing ◽  
Pcr Rflp ◽  
Polymerase Chain

Fluorescent Pseudomonas spp. isolated from fruiting bodies (FB) of Cantharellus cibarius were characterized physiologically and genetically and were compared with fluorescent Pseudomonas from forest soil and with sequences from the GenBank database. Pseudomonas spp. from FB differed physiologically from isolates from soil lacking FB and had some similarities with the strains obtained from soil underneath the FB. Analyses of the polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) patterns and partial sequencing analysis of the 16S-rDNA region indicated that the bacteria isolated from these environments were different. However, there was no specific Pseudomonas genotype restricted to the FB environment. Utilization of the reported fungal exudates trehalose and mannitol may explain how millions of bacteria survive in the C. cibarius FB without deteriorating the fungal mycelium. The importance of the metabolic characterization of bacteria and the possible mechanisms involved in the association with C. cibarius are discussed. Our study showed that standard processes for bacterial identification, e.g., Biolog®and 16S-rDNA are insufficient until databases for different ecosystems are created.Key words: Cantharellus cibarius, fluorescent Pseudomonas, carbon utilization, PCR–RFLP, 16S-rDNA sequencing.

scholarly journals ISOLATION, PURIFICATION, AND CHARACTERIZATION OF LIPASE FROM BACILLUS SP. FROM KITCHEN GREASE

2018 ◽  
Vol 11 (6) ◽  
pp. 224
Author(s):  
Jaiganesh R ◽  
Jaganathan Mk
Keyword(s):  
16S Rdna ◽  
Solvent Tolerance ◽  
16S Rdna Sequencing ◽  
Bacillus Sp ◽  
Purification And Characterization ◽  
Lipase Enzyme ◽  
Sequencing Method ◽  
Rdna Sequencing ◽  
Solvent Tolerant

Objective: The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. The objective of this work was to isolation, purification and characterization of solvent tolerant lipase from Bacillus sp. from kitchen grease for a variety of applications including organic synthetic reactions and preparation of enantiomerically pure pharmaceuticals.Methods: Lipase producing isolates were screened from kitchen grease on a selective medium rhodamine B olive oil agar, and tributyrin agar was used to screen the lipase and esterase producing an organism, respectively. The isolate identified using 16S rDNA sequencing method and enzyme activity was quantitatively assayed. Lipase production was characterized in different conditions.Results: The isolate showed highest lipase activity was which later was identified as Bacillus sp. using 16S rDNA sequencing method. The lipase was purified using ammonium sulfate precipitation. The isolate showed excellent tolerance to methanol, ethanol, acetonitrile, and moderate tolerance to butanol. The increased biomass concentration, maximum production, and activity were achieved at 37°C in 24 h incubation, then gradual reduction in production was observed. The maximum activity of lipase enzyme was obtained at pH between 6 and 9.Conclusion: The isolate produce solvent tolerance lipase enzyme and it can be a promising candidate of solvent tolerance lipase enzyme for variety of industrial applications.

scholarly journals Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis

2006 ◽  
Vol 41 (10) ◽  
pp. 1507-1516 ◽  
Author(s):  
Érico Leandro da Silveira ◽  
Rodrigo Matheus Pereira ◽  
Denilson César Scaquitto ◽  
Eliamar Aparecida Nascimbém Pedrinho ◽  
Silvana Pómpeia Val-Moraes ◽  
...  
Keyword(s):  
Bacterial Diversity ◽  
16S Rdna ◽  
Phylogenetic Analyses ◽  
Chemical Properties ◽  
Pcr Primers ◽  
Native Forest ◽  
Sequencing Analysis ◽  
Soil Dna ◽  
Rdna Sequencing ◽  
The Impact

Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity. From the NFA soil 134 clones were analyzed, while 116 clones were analyzed from the EAA soil samples. The sequences were compared with those online at the GenBank. Phylogenetic analyses revealed differences between the soil types and high diversity in both communities. Soil from the Eucalyptus spp. arboretum was found to have a greater bacterial diversity than the soil investigated from the native forest area.

Identification ofEnterococcus sp. from midgut of silkworm based on biochemical and 16S rDNA sequencing analysis

2006 ◽  
Vol 56 (3) ◽  
pp. 201-205 ◽  
Author(s):  
Chen Fei ◽  
Xing-meng Lu ◽  
Yong-hua Qian ◽  
Haiyan Zhang ◽  
Qaisar Mahmood
Keyword(s):  
16S Rdna ◽  
Sequencing Analysis ◽  
16S Rdna Sequencing ◽  
Rdna Sequencing

Identification of the Dominant Microbial Species of Spoiled Crisp Grass Carp (Ctenopharyngodon idellus C. et V.) and Grass Carp (Ctenopharyngodon idellus) Fillets during Cold Storage by Culture-Independent 16S rDNA Sequence Analysis

2017 ◽  
Vol 81 (1) ◽  
pp. 84-92 ◽  
Author(s):  
Lin Li ◽  
Ziqiang Pan ◽  
Zhihua Shen
Keyword(s):  
16S Rdna ◽  
Clone Library ◽  
Grass Carp ◽  
Rflp Analysis ◽  
Ctenopharyngodon Idellus ◽  
Total Volatile ◽  
16S Rdna Clone Library ◽  
Pseudomonas Spp ◽  
Rdna Sequencing ◽  
Rdna Clone

ABSTRACT A new freshwater cultivation species, crisp grass carp (CGC; Ctenopharyngodon idellus C. et V.) has a special texture and is popular with consumers; thus, we should pay close attention to its storage conditions and bacterial degradation. CGC and grass carp (GC; Ctenopharyngodon idellus) were commercialized as fillets and subsequently stored at 4 and 8°C. Microbial growth parameters (total viable counts, psychrotrophic bacteria, and Pseudomonas spp.), physicochemical data (pH and total volatile base nitrogen), and sensory analysis were monitored during the storage period. Dominant microorganisms were identified using a 16S rDNA clone library and restriction fragment length polymorphism (RFLP) analysis after the fillets had spoiled completely. The results showed that Pseudomonas spp. lagged behind the psychrotrophic population and the total viable counts initially and increased more rapidly after storage for 2 days. Total volatile base nitrogen contents were found to increase with storage time in both species, coinciding with ongoing microbial change. The pH results obtained for both species during storage showed an overall increase at the end of storage. Sensory evaluation showed a shelf life of 3 and 6 days for both species at 8 and 4°C, respectively. RFLP analysis of the 16S rDNA clone library revealed that there were seven and five distinct RFLP pattern groups in the microflora of the spoiled CGC and GC fillets, respectively. Through RFLP patterns and 16S rDNA sequencing from the clones, it was determined that CGC fillets stored at 4°C were dominated by Pseudomonas spp. at the point of sensory rejection, whereas GC fillets were dominated by populations affiliated with Pseudomonas sp., Acinetobacter sp., and Aeromonas sp.

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