Extracellular peroxidase production by Coprinus species from urea-treated soil

2004 ◽  
Vol 50 (1) ◽  
pp. 57-60 ◽  
Author(s):  
Keisuke Ikehata ◽  
Ian D Buchanan ◽  
Daniel W Smith
Keyword(s):  
Wastewater Treatment ◽  
Peroxidase Activity ◽  
Liquid Culture ◽  
Malt Extract ◽  
Urea Treatment ◽  
Treated Soil ◽  
Extracellular Peroxidase ◽  
Shake Flasks ◽  
Inky Cap ◽  
Phenol Wastewater

Thirteen strains of inky-cap mushroom Coprinus species were evaluated for the production of extracellular peroxidase. The liquid fermentation was carried out in shake flasks containing 1% glucose, 0.5% peptone, 0.3% yeast extract, and 0.3% malt extract broth at 25 °C. Peroxidase activity was detected in the liquid culture of several Coprinus species, including C. echinosporus NBRC 30630; C. macrocephalus NBRC 30117; Coprinus spp. UAMH 10065, UAMH 10066, UAMH 10067, and 074, after 10 days of growth. Peroxidase production by Coprinus sp. UAMH 10067, a Coprinus species isolated from urea-treated soil, was comparable to that of C. cinereus and reached 15 U·mL–1 after 10 days. In addition, the peroxidase from Coprinus sp. UAMH 10067 was apparently more thermally stable than the enzyme produced by C. cinereus.Key words: Coprinus species, urea treatment, phenol, wastewater treatment.

Phytochrome-mediated effects on extracellular peroxidase activity, lignin content and bending resistance in etiolated Vicia faba epicotyls

1994 ◽  
Vol 92 (4) ◽  
pp. 555-562 ◽  
Author(s):  
Jorge J. Casal ◽  
R. Alejandra Mella ◽  
Carlos L. Ballare ◽  
Sara Maldonado
Keyword(s):  
Vicia Faba ◽  
Peroxidase Activity ◽  
Lignin Content ◽  
Extracellular Peroxidase ◽  
Mediated Effects ◽  
Bending Resistance

scholarly journals Research on phenol wastewater treatment by low-temperature multi-effect distillation combining with super-critical water oxidation

2018 ◽  
Vol 208 ◽  
pp. 012063
Author(s):  
Cuijie Jia ◽  
Bo Yuan ◽  
Fengming Zhang ◽  
Chuan He ◽  
Chuangjian Su ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Low Temperature ◽  
Water Oxidation ◽  
Phenol Wastewater

Highly enhanced electrocatalytic activity of nano-TiO2/Ti membrane electrode for phenol wastewater treatment

2020 ◽  
Vol 31 (16) ◽  
pp. 13511-13520
Author(s):  
Jiaxin Xu ◽  
Xiaoping Liang ◽  
Xiaowei Fan ◽  
Yuxi Song ◽  
Zenghua Zhao ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Electrocatalytic Activity ◽  
Membrane Electrode ◽  
Nano Tio2 ◽  
Phenol Wastewater

In vitro liquid culture and optimization of Steinernema jeffreyense using shake flasks

BioControl ◽  
2019 ◽  
Vol 65 (2) ◽  
pp. 223-233 ◽  
Author(s):  
Murray D. Dunn ◽  
Prasanna D. Belur ◽  
Antoinette P. Malan
Keyword(s):  
Liquid Culture ◽  
Shake Flasks

Extracellular peroxidase activity at the hyphal tips of the white-rot fungus Phanerochaete crassa WD1694

2006 ◽  
Vol 52 (5) ◽  
pp. 429-435 ◽  
Author(s):  
Mariko Takano ◽  
Hisashi Abe ◽  
Noriko Hayashi
Keyword(s):  
Peroxidase Activity ◽  
White Rot ◽  
White Rot Fungus ◽  
Extracellular Peroxidase

Cell growth and pigment production in suspension cultures of a mycobiont isolated from the lichen Cladonia cristaiella

1995 ◽  
Vol 73 (S1) ◽  
pp. 590-594 ◽  
Author(s):  
Y. Yamamoto ◽  
Y. Kinoshita ◽  
T. Kurokawa ◽  
I. Yoshimura ◽  
V. Ahmadjian ◽  
...  
Keyword(s):  
Cell Growth ◽  
Carbon Source ◽  
Nitrogen Source ◽  
Liquid Culture ◽  
Pigment Production ◽  
Malt Extract ◽  
Nutritional Factors ◽  
Cultural Conditions ◽  
Acetone Extracts ◽  
Affect Cell Growth

This is the first study on the factors that affect cell growth and the production of secondary metabolites of a lichen mycobiont in liquid culture. An ascospore-derived strain of Cladonia cristatella mycobiont accumulated and excreted red pigments into a liquid medium. Growth of the mycobiont was increased by using liquid Lilly–Barnett medium containing 16% (w/v) sucrose as a carbon source, 0.2% (w/v) L-glutamine as a nitrogen source, and 0.2% (w/v) polypeptone, adjusting pH to 5.0 before autoclaving, and incubating cultures at 20 °C. Pigment production by the mycobiont was increased by using liquid Lilly–Barnett medium containing 4% (w/v) sucrose as a carbon source, 0.2% (w/v) L-asparagine as a nitrogen source, and 0.2% (w/v) malt extract, adjusting pH to 5.0 before autoclaving and incubating cultures at 20 °C. All acetone extracts under any cultural conditions yielded similar HPLC chromatograms. We proved no relationship between cell growth and secondary metabolism based on the nutritional factors in the cultured C. cristatella mycobiont. Key words: lichen, suspension culture, Cladonia cristatella mycobiont, red pigment, production, and growth factor.

scholarly journals SIMPLE SCREENING FOR POTENTIAL CHRYSENE DEGRADING FUNGI

2015 ◽  
Vol 2 (1) ◽  
pp. 364 ◽  
Author(s):  
Asep Hidayat ◽  
Sanro Tachibana
Keyword(s):  
Liquid Culture ◽  
Sea Water ◽  
Soil Samples ◽  
Malt Extract ◽  
Benzene Rings ◽  
Polycyclic Aromatic ◽  
Degradation Activity ◽  
Potential Activity ◽  
Fusarium Sp ◽  
Simple Screening

<p>Chrysene is a class of organic compounds, arranged in four benzene rings, and a polycyclic aromatic hydrocarbon (PAH), It has been found to have a variety of toxicity, mutagenicity, teratogenicity, and carcinogenicity on microorganisms, plants and animals in environment. Nowadays, the most attention on degradation of PAHs is investigating degradation of high-molecular-weight molecules. However, microbes which have ability to degrade PAHs containing more than three benzene rings are more difficult to be obtained. In order to provide chrysene degrading fungi, this study was conducted for screening, and isolating the fungi from soil, and hence investigating the selected fungi having high chrysene degradation activity. From the 62 soil samples collected from Matsuyama-Japan, 92 isolates were found and 20 isolates of them grew well in Malt extract media contaminated with chrysene (covered up 90%). Among them, a fungus, Fusarium sp. has the highest activity to degrade chrysene compared to others screened fungi. This fungus was evaluated further on liquid medium from distilled water and sea water to confirm their validity in degrading chrysene. The result showed that Fusarium sp. F092 degraded 48% of chrysene, where the chrysene degradation showed no differences at salinity of 35o/oo. The effect of variation of enzymes activities on incubation times was evaluated simultaneously. When the fungus was grown in a liquid culture, the highest activity of 1,2-dioxygenase reached 203.5 UL-1 were observed on 30 days incubation and 29.7 Ul-1 for 2,3-dioxygenase on 40 days incubation. The products of chrysene degradation by Fusarium sp. F092 are, 1-hydroxy 2-napthoic acid and catechol. In conclusion, Fusarium sp. F092 shows a high potential activity degrade PAHs contamination .</p><p><br /><strong>Keywords</strong>: Screening, chrysene, bioegradation, Fusarium sp. F092</p>

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