Ca2+fluxes in developingTrichoderma viridemycelium

2000 ◽  
Vol 46 (4) ◽  
pp. 312-324 ◽  
Author(s):  
Martin ŠŠimkovič ◽  
Svetlana Kryšštofová ◽  
L'udovít Varečka
Keyword(s):  
Life Cycle ◽  
Energy Metabolism ◽  
Microsomal Fraction ◽  
Trichoderma Viride ◽  
Mitochondrial Fraction ◽  
The Other ◽  
Submerged Cultivation ◽  
Transport Systems ◽  
Mycelium Growth ◽  
X Ray

The properties of both Ca2+influx and efflux in the mycelium during the life cycle of Trichoderma viride were studied by means of45Ca2+and by X-ray fluorescence spectroscopy measurements. The properties of the45Ca2+influx and effluxes indicate that they are mediated by different transport systems. The Ca2+influx could be mediated by an electrogenic Ca2+/nH+antiport, or by an Ca2+uniport system. Both Ca2+influx and efflux were stimulated by the uncouplers (and the treatment leading to the suppression of energy metabolism) and by azalomycin F, an antifungal agent. Salicylate stimulated the Ca2+efflux, but inhibited the Ca2+influx. In the isolated preparation of crude vacuolar/mitochondrial fraction, salicylate induced the Ca2+release, as did A23187. Azalomycin F moderately released Ca2+from the microsomal fraction. On the other hand, uncouplers did not release Ca2+from the isolated organelles, but inhibited to a different extent the ATP-dependent and -independent Ca2+influx. The results could be explained in terms of the capacitative Ca2+influx mechanism. The rate of45Ca2+influx, or of the40Ca2+content, was maximal after about 30 h of submerged cultivation, and then decreased. The results show that loading of internal Ca2+stores occurs in the early stages of the development of mycelium only, and the Ca2+influx mechanism is developmentally down-regulated, being almost nonexistent during its later stages. In older mycelium, growth seems to be autonomous of the extracellular Ca2+until the onset of conidiation.Key words: Trichoderma viride, development, Ca2+influx, Ca2+efflux, salicylate, uncoupler, azalomycin F.

scholarly journals The metabolism of phospholipids in mouse brain slices

1966 ◽  
Vol 101 (3) ◽  
pp. 674-679 ◽  
Author(s):  
PA Clayton ◽  
CE Rowe
Keyword(s):  
Phosphatidic Acid ◽  
Mouse Brain ◽  
Grey Matter ◽  
Microsomal Fraction ◽  
Brain Slices ◽  
Mitochondrial Fraction ◽  
Subcellular Fractions ◽  
The Other ◽  
Metabolic Inhibitors ◽  
Radioactive Labelling

1. Slices of mouse brain grey matter were incubated with [(32)P]phosphate and [1-(14)C]acetate. Doubly labelled phospholipids were extracted from subcellular fractions prepared from the slices in a mixture of metabolic inhibitors, under conditions where there was negligible change in radioactive labelling during the preparation. Two tissue fractions were studied in detail; one contained a high proportion of mitochondria and the other was mainly microsomal. 2. In all tissue fractions the highest incorporations of both [(32)P]phosphate and [1-(14)C]acetate occurred into phosphatidylcholine. 3. After incubation for 1hr., the (32)P/(14)C ratios for phosphatidylcholine, phosphatidylethanolamine and phosphatidic acid in the mitochondrial fraction were similar to those in the microsomal fraction. 4. The (32)P/(14)C ratios were similar in phosphatidylcholine and phosphatidylethanolamine and much lower than those in phosphatidic acid and phosphatidylinositol.

Microchemical imaging in biomedical research

1992 ◽  
Vol 50 (2) ◽  
pp. 1118-1119
Author(s):  
P. Ingram
Keyword(s):  
Data Analysis ◽  
Electron Probe ◽  
The Other ◽  
X Ray ◽  
Cell Element ◽  
Physiological Information ◽  
Functional States ◽  
Elemental Maps ◽  
Electron Energy Loss ◽  
Secondary Ion

It is well established that unique physiological information can be obtained by rapidly freezing cells in various functional states and analyzing the cell element content and distribution by electron probe x-ray microanalysis. (The other techniques of microanalysis that are amenable to imaging, such as electron energy loss spectroscopy, secondary ion mass spectroscopy, particle induced x-ray emission etc., are not addressed in this tutorial.) However, the usual processes of data acquisition are labor intensive and lengthy, requiring that x-ray counts be collected from individually selected regions of each cell in question and that data analysis be performed subsequent to data collection. A judicious combination of quantitative elemental maps and static raster probes adds not only an additional overall perception of what is occurring during a particular biological manipulation or event, but substantially increases data productivity. Recent advances in microcomputer instrumentation and software have made readily feasible the acquisition and processing of digital quantitative x-ray maps of one to several cells.

The Electron Effect of Aromatic Group: Control Conformation of 2-Aromatic Cyclododecanone Derivatives

2020 ◽  
Vol 24 (10) ◽  
pp. 1139-1147
Author(s):  
Yang Mingyan ◽  
Wang Daoquan ◽  
Wang Mingan
Keyword(s):  
1H Nmr ◽  
13C Nmr ◽  
Nmr Spectra ◽  
Coupling Constants ◽  
The Other ◽  
Repulsive Interaction ◽  
X Ray Diffraction ◽  
X Ray ◽  
Electron Effect ◽  
Group Control

2-Phenylcyclododecanone and 2-cyclohexylcyclododecanone derivatives were synthesized and characterized by 1H NMR, 13C NMR, HR-ESI-MS and X-ray diffraction. Their preferred conformations were analyzed by the coupling constants in the 1H NMR spectra and X-ray diffraction, which showed the skeleton ring of these derivatives containing [3333]-2-one conformation, and the phenyl groups were located at the side-exo position of [3333]-2-one conformation due to the strong π-π repulsive interaction between the π- electron of benzene ring and π-electron of carbonyl group. The cyclohexyl groups were located at the corner-syn or the side-exo position of [3333]-2-one conformation depending on the hindrance of the other substituted groups. The π-π electron effect played a crucial role in efficiently controlling the preferred conformation of 2-aromatic cyclododecanone and the other 2-aromatic macrocyclic derivatives with the similar preferred square and rectangular conformations.

scholarly journals Adhesion of Triple-Negative Breast Cancer Cells under Fluorescent and Soft X-ray Contact Microscopy

2021 ◽  
Vol 22 (14) ◽  
pp. 7279
Author(s):  
Paulina Natalia Osuchowska ◽  
Przemysław Wachulak ◽  
Wiktoria Kasprzycka ◽  
Agata Nowak-Stępniowska ◽  
Maciej Wakuła ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
Cell Imaging ◽  
Triple Negative ◽  
The Other ◽  
Promising Technique ◽  
X Ray ◽  
Microscopy Technique ◽  
Cancer Cell Adhesion

Understanding cancer cell adhesion could help to diminish tumor progression and metastasis. Adhesion mechanisms are currently the main therapeutic target of TNBC-resistant cells. This work shows the distribution and size of adhesive complexes determined with a common fluorescence microscopy technique and soft X-ray contact microscopy (SXCM). The results presented here demonstrate the potential of applying SXCM for imaging cell protrusions with high resolution when the cells are still alive in a physiological buffer. The possibility to observe the internal components of cells at a pristine and hydrated state with nanometer resolution distinguishes SXCM from the other more commonly used techniques for cell imaging. Thus, SXCM can be a promising technique for investigating the adhesion and organization of the actin cytoskeleton in cancer cells.

scholarly journals Proteogenomic Insights into the Physiology of Marine, Sulfate-Reducing, Filamentous Desulfonema limicola and Desulfonema magnum

2021 ◽  
Vol 31 (1) ◽  
pp. 36-56
Author(s):  
Vanessa Schnaars ◽  
Lars Wöhlbrand ◽  
Sabine Scheve ◽  
Christina Hinrichs ◽  
Richard Reinhardt ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Gene Clusters ◽  
Transport Systems ◽  
Natural Habitats ◽  
Sulfate Reducing ◽  
Type Iv ◽  
Aromatic Compound Degradation ◽  
High Degree ◽  
Reducing Bacteria ◽  
Gliding Movement

The genus Desulfonema belongs to the deltaproteobacterial family Desulfobacteraceae and comprises marine, sulfate-reducing bacteria that form filaments and move by gliding. This study reports on the complete, manually annotated genomes of Dn. limicola 5ac10T (6.91 Mbp; 6,207 CDS) and Dn. magnum 4be13T (8.03 Mbp; 9,970 CDS), integrated with substrate-specific proteome profiles (8 vs. 11). The richness in mobile genetic elements is shared with other Desulfobacteraceae members, corroborating horizontal gene transfer as major driver in shaping the genomes of this family. The catabolic networks of Dn. limicola and Dn. magnum have the following general characteristics: 98 versus 145 genes assigned (having genomic shares of 1.7 vs. 2.2%), 92.5 versus 89.7% proteomic coverage, and scattered gene clusters for substrate degradation and energy metabolism. The Dn. magnum typifying capacity for aromatic compound degradation (e.g., p-cresol, 3-phenylpropionate) requires 48 genes organized in operon-like structures (87.7% proteomic coverage; no homologs in Dn. limicola). The protein complements for aliphatic compound degradation, central pathways, and energy metabolism are highly similar between both genomes and were identified to a large extent (69–96%). The differential protein profiles revealed a high degree of substrate-specificity for peripheral reaction sequences (forming central intermediates), agreeing with the high number of sensory/regulatory proteins predicted for both strains. By contrast, central pathways and modules of the energy metabolism were constitutively formed under the tested substrate conditions. In accord with their natural habitats that are subject to fluctuating changes of physicochemical parameters, both Desulfonema strains are well equipped to cope with various stress conditions. Next to superoxide dismutase and catalase also desulfoferredoxin and rubredoxin oxidoreductase are formed to counter exposure to molecular oxygen. A variety of proteases and chaperones were detected that function in maintaining cellular homeostasis upon heat or cold shock. Furthermore, glycine betaine/proline betaine transport systems can respond to hyperosmotic stress. Gliding movement probably relies on twitching motility via type-IV pili or adventurous motility. Taken together, this proteogenomic study demonstrates the adaptability of Dn. limicola and Dn. magnum to its dynamic habitats by means of flexible catabolism and extensive stress response capacities.

scholarly journals X-ray variability of the HMXB Cen X-3: evidence for inhomogeneous accretion flows.

2020 ◽  
Author(s):  
G Sanjurjo-Ferrín ◽  
J M Torrejón ◽  
K Postnov ◽  
L Oskinova ◽  
J J Rodes-Roca ◽  
...  
Keyword(s):  
Equivalent Width ◽  
Compact Star ◽  
Timing Analysis ◽  
Roche Lobe ◽  
The Other ◽  
Emission Lines ◽  
High Mass ◽  
X Ray ◽  
Accretion Flows ◽  
Inner Radius

Abstract Cen X-3 is a compact high mass X-ray binary likely powered by Roche lobe overflow. We present a phase-resolved X-ray spectral and timing analysis of two pointed XMM-Newton observations. The first one took place during a normal state of the source, when it has a luminosity LX ∼ 1036 erg s−1. This observation covered orbital phases φ = 0.00 − 0.37, i.e. the egress from the eclipse. The egress lightcurve is highly structured, showing distinctive intervals. We argue that different intervals correspond to the emergence of different emitting structures. The lightcurve analysis enables us to estimate the size of such structures around the compact star, the most conspicuous of which has a size ∼0.3R*, of the order of the Roche lobe radius. During the egress, the equivalent width of Fe emission lines, from highly ionized species, decreases as the X-ray continuum grows. On the other hand, the equivalent width of the Fe Kα line, from near neutral Fe, strengthens. This line is likely formed due to the X-ray illumination of the accretion stream. The second observation was taken when the source was 10 times X-ray brighter and covered the orbital phases φ = 0.36 − 0.80. The X-ray lightcurve in the high state shows dips. These dips are not caused by absorption but can be due to instabilities in the accretion stream. The typical dip duration, of about 1000 s, is much longer than the timescale attributed to the accretion of the clumpy stellar wind of the massive donor star, but is similar to the viscous timescale at the inner radius of the accretion disk.

scholarly journals Determination of the Height of Hard X-Ray Sources in the Solar Atmosphere by Measurement of Photospheric Albedo Photons

1975 ◽  
Vol 68 ◽  
pp. 239-241
Author(s):  
John C. Brown ◽  
H. F. Van Beek
Keyword(s):  
Solar Atmosphere ◽  
The Other ◽  
X Ray ◽  
Theoretical Predictions ◽  
Source Models ◽  
The One

SummaryThe importance and difficulties of determining the height of hard X-ray sources in the solar atmosphere, in order to distinguish source models, have been discussed by Brown and McClymont (1974) and also in this Symposium (Brown, 1975; Datlowe, 1975). Theoretical predictions of this height, h, range between and 105 km above the photosphere for different models (Brown and McClymont, 1974; McClymont and Brown, 1974). Equally diverse values have been inferred from observations of synchronous chromospheric EUV bursts (Kane and Donnelly, 1971) on the one hand and from apparently behind-the-limb events (e.g. Datlowe, 1975) on the other.

scholarly journals White Dwarf Masses and Accretion Rates of Recurrent Novae: an X-ray Perspective

2011 ◽  
Vol 7 (S281) ◽  
pp. 186-189
Author(s):  
Koji Mukai ◽  
Jennifer L. Sokoloski ◽  
Thomas Nelson ◽  
Gerardo J. M. Luna
Keyword(s):  
White Dwarf ◽  
Cataclysmic Variables ◽  
The Other ◽  
X Ray ◽  
Other Hand ◽  
Accretion Rates

AbstractWe present recent results of quiescent X-ray observations of recurrent novae (RNe) and related objects. Several RNe are luminous hard X-ray sources in quiescence, consistent with accretion onto a near Chandrasekhar mass white dwarf. Detection of similar hard X-ray emissions in old novae and other cataclysmic variables may lead to identification of additional RNe candidates. On the other hand, other RNe are found to be comparatively hard X-ray faint. We present several scenarios that may explain this dichotomy, which should be explored further.

scholarly journals A preliminary study on antifungal effect of TiO2-based paints in natural indoor light

2010 ◽  
Vol 12 (4) ◽  
pp. 53-57 ◽  
Author(s):  
Agata Markowska-Szczupak ◽  
Krzysztof Ulfig ◽  
Barbara Grzmil ◽  
Antoni Morawski
Keyword(s):  
Antifungal Activity ◽  
Aspergillus Niger ◽  
Trichoderma Viride ◽  
Photocatalytic Reaction ◽  
X Ray Diffraction ◽  
Antifungal Effect ◽  
X Ray ◽  
Preliminary Study ◽  
Crystalline Forms ◽  
Growth Of Fungi

A preliminary study on antifungal effect of TiO2-based paints in natural indoor light The antifungal activity of four commercial photocatalytic paints (KEIM Ecosil ME, Titanium FA, Photo Silicate and Silicate D) in natural indoor light was investigated. The paints contained TiO2 in rutile and anatase crystalline forms as evidenced by means of the X-ray diffraction analysis. In most cases the paints inhibited growth of fungi viz. Trichoderma viride, Aspergillus niger, Coonemeria crustacea, Eurotium herbariorum, and Dactylomyces sp. The KEIM Ecosil ME paint displayed the highest antifungal effect in the light, which could be explained with the highest anatase content. The paint antifungal activity and the fungal sensitivity to the TiO2-mediated photocatalytic reaction both decreased in the following orders: KEIM Ecosil ME > Titanium FA > Photo Silicate > Silicate D and T. viride > Dactylomyces sp. > A. niger > E. herbariorum.

PHASE TRANSITION IN LAYERED PEROVSKITE LIKE MANGANATE Ca3Mn2O7 UNDER HIGH PRESSURE

2001 ◽  
Vol 15 (18) ◽  
pp. 2491-2497 ◽  
Author(s):  
J. L. ZHU ◽  
L. C. CHEN ◽  
R. C. YU ◽  
F. Y. LI ◽  
J. LIU ◽  
...  
Keyword(s):  
Crystal Structure ◽  
High Pressure ◽  
Diamond Anvil Cell ◽  
The Other ◽  
Layered Perovskite ◽  
X Ray Diffraction ◽  
Two Phase ◽  
X Ray ◽  
Diamond Anvil

In situ high pressure energy dispersive X-ray diffraction measurements on layered perovskite-like manganate Ca 3 Mn 2 O 7 under pressures up to 35 GPa have been performed by using diamond anvil cell with synchrotron radiation. The results show that the structure of layered perovskite-like manganate Ca 3 Mn 2 O 7 is unstable under pressure due to the easy compression of NaCl-type blocks. The structure of Ca 3 Mn 2 O 7 underwent two phase transitions under pressures in the range of 0~35 GPa. One was at about 1.3 GPa with the crystal structure changing from tetragonal to orthorhombic. The other was at about 9.5 GPa with the crystal structure changing from orthorhombic back to another tetragonal.

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