Genetic structure and mating system of white spruce (Piceaglauca) in a seed production area

1984 ◽  
Vol 14 (5) ◽  
pp. 639-643 ◽  
Author(s):  
John N. King ◽  
Bruce P. Dancik ◽  
Narinder K. Dhir
Keyword(s):  
Genetic Structure ◽  
Mating System ◽  
Seed Production ◽  
Gel Electrophoresis ◽  
Random Mating ◽  
White Spruce ◽  
Starch Gel Electrophoresis ◽  
Starch Gel ◽  
Production Area ◽  
Mating Model

Embryos and megagametophytes of open-pollinated seed of 37 white spruce (Piceaglauca (Moench) Voss) trees from a seed production area were analyzed by starch gel electrophoresis to determine the genetic structure and mating system over 2 seed crop years. Analysis of four polymorphic enzyme loci (Gdh, Idh, Pgm, and Pgi-2) for spatial and temporal genetic structure and mating system indicated substantial deviations from the random mating model that is assumed when open-pollinated families are designated as half-sibs.

Mating system of Racosperma auriculiforme in a seed production area in Zaire

1993 ◽  
Vol 71 (6) ◽  
pp. 779-785 ◽  
Author(s):  
P. Damase Khasa ◽  
William M. Cheliak ◽  
Jean Bousquet
Keyword(s):  
Mating System ◽  
Seed Production ◽  
Random Mating ◽  
Single Locus ◽  
Acacia Auriculiformis ◽  
Mixed Mating ◽  
Outcrossing Rates ◽  
Production Area ◽  
Mixed Mating Model ◽  
Mating Model

Analysis of the mating system of Racosperma auriculiforme (formerly Acacia auriculiformis) in a seed production area in Zaire was conducted using a mixed mating model at five polymorphic loci (Est-2, Est-3, G6p-dh, Nadhdh-2, Pgm-1). Single-locus estimates of outcrossing rate varied widely between 0.615 and 1.204 and were significantly heterogenous among and within the stands. Weighted means of single-locus estimates of outcrossing rates (ts) ranged from 0.859 to 1.032 while multilocus estimates of outcrossing rates (tm) ranged from 0.863 to 1.059. Comparisons between the single-locus estimates and the multilocus estimates of outcrossing rates indicated that most of the inbreeding detected was due to consanguineous (sibling) matings rather than selfing. Comparisons of observed and expected inbreeding coefficients indicated a general deficit of heterozygotes over the frequencies expected from random mating in the single stands and the pooled stands. Locus to locus variation in outcrossing rates suggested that violation of the mixed mating model might have occurred. Implications for seed production, collection, and distribution for reforestation are discussed. Key words: Acacia, mating system, Racosperma, seed production area, Zaire.

scholarly journals Estructura genética de poblaciones de Eriocaulon bilobatum (Eriocaulaceae): una especie amenazada de humedades temporales

2019 ◽  
Vol 85 ◽  
pp. 81
Author(s):  
Fabiola Magallán Hernández ◽  
Mahinda Martínez ◽  
Luis Hernández Sandoval ◽  
Ken Oyama
Keyword(s):  
Genetic Diversity ◽  
Life History ◽  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Life History Traits ◽  
Aquatic Environments ◽  
Starch Gel Electrophoresis ◽  
Temporary Wetlands ◽  
Sexual And Asexual Reproduction ◽  
Starch Gel

<em>Eriocaulon bilobatum</em> is an aquatic species that inhabits temporary wetlands in central Mexico. It is annual, herbaceous, emergent, with sexual and asexual reproduction, monoecious and insect pollinated. It is a rare and vulnerable species due to its endangered habitats. The objectives of this study were to determine the diversity and genetic structure of <em>E. bilobatum </em> and to know if there is a correlation with genetic diversity and its ecological and life history traits. Using horizontal starch-gel electrophoresis, we screened 160 individuals from four populations. <em>E. bilobatum</em> has a higher genetic diversity (A=2.32, Ae=1.31, P=69.65, Ho=0.134, He=0.197, HT=0.221) than species with similar ecological and life history traits, moderate levels of inbreeding (FIS = 0.312) and low genetic differentiation among populations (FST = 0.053 y GST = 0.048). Its diversity and genetic structure are determined by the mating system and life history traits, more than by inhabiting aquatic environments.

A Genetic Comparison of Sympatric Populations of Sand Lance (Genus Ammodytes) from the Region East of Cape Soya, Japan

1989 ◽  
Vol 46 (11) ◽  
pp. 1945-1951 ◽  
Author(s):  
Hiroaki Okamoto
Keyword(s):  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Distinct Species ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Sympatric Populations ◽  
Sand Lance ◽  
Protein Coding ◽  
Starch Gel ◽  
Genetic Comparison

Sand fance (Genus Ammodytes) collected from four stations off Japan and one station at Kodiak, Alaska were genetically characterized at 17 protein coding loci using starch-gel electrophoresis. Sand lance in Wakkanai (Cape Soya, Japan) consist of two genetically distinct groups. They are fixed for different alleles at four loci (Ldh-2, -3, G3pdh-2, and Mdhp-2). The genetic structure of one of the groups (Wakkanai-a group, W-a) is similar to that of A. personatus around Japan. The other group (Wakkanai-b group, W-b) has different genetic structure from either A. personatus or the Alaskan collection, which is presumed to belong to A. hexapterus. It is not presently possible to identify the affiliation of the W-b group; however, despite its sympatry with the W-a group, it is reproductively isolated and therefore is probably a distinct species occurring northeast of Hokkaido.

Sibling species in the mangrove genus Ceriops (Rhizophoraceae), detected using biochemical genetics*

1988 ◽  
Vol 1 (4) ◽  
pp. 391 ◽  
Author(s):  
ER Ballment ◽  
TJ III Smith ◽  
JA Stoddart
Keyword(s):  
Genetic Structure ◽  
Sibling Species ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Biochemical Genetics ◽  
Species Status ◽  
Phenotypic Characters ◽  
Starch Gel ◽  
Ceriops Tagal ◽  
Mangrove Trees

Starch gel electrophoresis of enzymes from the mangrove trees Ceriops tagal (Perr.) C. B. Robinson var. tagal, Ceriops tagal (Perr.) C. B. Robinson var. australis C. T. White, and Ceriops decandra (Griff.) Ding Hou in northern Queensland, Australia, revealed a uniform genetic structure within taxa, but high levels of genetic divergence among taxa. No more than 20% of enzyme phenotypic characters were shared by any pair of the taxa, and patterns of enzyme phenotypic variation consistent with hybridisation among them were not seen in any samples, including those from areas of spmpatry. This evidence of reproductive isolation is considered sufficient to accord species status to C. tagal var. australis.* Aust. lnst. Marine Sci. Contrib. No. 453.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

STARCH GEL ELECTROPHORESIS OF MYOGEN FROM CHICKEN BREAST MUSCLE IN CONSTANT AND GRADIENT BUFFER SYSTEMS

1963 ◽  
Vol 41 (1) ◽  
pp. 369-387 ◽  
Author(s):  
J. M. Neelin
Keyword(s):  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Breast Muscle ◽  
Chicken Breast ◽  
Starch Gel Electrophoresis ◽  
Two Dimensional ◽  
Sodium Cacodylate ◽  
Gradient Systems ◽  
Optimal Separation ◽  
Starch Gel

By varying conditions of starch gel electrophoresis, factors contributing to the resolution of myogen proteins from chicken breast muscle have been studied. Variables examined included composition of the myogen extractant, protein concentration, ionic strength of electrophoretic media, pH of gel media, plane and direction of electrophoresis, and the nature of cations and anions in gel media and bridge solutions. The significance of anions was more closely studied with constant buffer systems, and gradient systems in which bridge electrolyte differed from, and gradually altered, the gel medium. Optimal separation was obtained in gradient systems with 0.10 M sodium chloride bridge solutions, and gel media of sodium cacodylate, pH 6.9, μ 0.010, which resolved 12 cationic zones, and sodium veronal, pH 7.4, μ 0.010, which resolved 10 anionic zones. These buffers in two-dimensional sequence revealed a total of about 24 components in this myogen.

Morphological and biochemical systematics of chubs, Nocomis biguttatus and N. micropogon (Pisces: Cyprinidae), in southern Ontario

1981 ◽  
Vol 59 (5) ◽  
pp. 771-775 ◽  
Author(s):  
Moira M. Ferguson ◽  
David L. G. Noakes ◽  
Roy G. Danzmann
Keyword(s):  
Gel Electrophoresis ◽  
Function Analysis ◽  
Morphological Differentiation ◽  
Sexually Dimorphic ◽  
Starch Gel Electrophoresis ◽  
Southern Ontario ◽  
Electrophoretic Mobilities ◽  
Starch Gel ◽  
Respective Species ◽  
Probability Of Misclassification

Examination of 17 presumptive gene loci by starch-gel electrophoresis revealed differential mobilities only at acid phosphatase-1, alcohol dehydrogenase, esterase-1, and phosphoglucomutase between Nocomis biguttatus and N. micropogon. No intraspecific variation was observed for any loci. The genetic identity (I) and genetic distance (D) were 0.874 and 0.134, respectively. The correlation of electrophoretic mobilities and nuptial tubercle pattern in sexually dimorphic males supports the present taxonomic distinction of these species and provides a simple, unambiguous means of identifying any individuals.Stepwise discriminant function analysis of a series of mensural characters was used to compare fish identified as to species by electrophoresis. At best this correctly assigned fish to their respective species in 85.7% of cases, with a probability of misclassification of 0.1335.This study suggests these two are sibling species, based on a comparison of biochemical and morphological differentiation.

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