scholarly journals Estructura genética de poblaciones de Eriocaulon bilobatum (Eriocaulaceae): una especie amenazada de humedades temporales

2019 ◽  
Vol 85 ◽  
pp. 81
Author(s):  
Fabiola Magallán Hernández ◽  
Mahinda Martínez ◽  
Luis Hernández Sandoval ◽  
Ken Oyama
Keyword(s):  
Genetic Diversity ◽  
Life History ◽  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Life History Traits ◽  
Aquatic Environments ◽  
Starch Gel Electrophoresis ◽  
Temporary Wetlands ◽  
Sexual And Asexual Reproduction ◽  
Starch Gel

<em>Eriocaulon bilobatum</em> is an aquatic species that inhabits temporary wetlands in central Mexico. It is annual, herbaceous, emergent, with sexual and asexual reproduction, monoecious and insect pollinated. It is a rare and vulnerable species due to its endangered habitats. The objectives of this study were to determine the diversity and genetic structure of <em>E. bilobatum </em> and to know if there is a correlation with genetic diversity and its ecological and life history traits. Using horizontal starch-gel electrophoresis, we screened 160 individuals from four populations. <em>E. bilobatum</em> has a higher genetic diversity (A=2.32, Ae=1.31, P=69.65, Ho=0.134, He=0.197, HT=0.221) than species with similar ecological and life history traits, moderate levels of inbreeding (FIS = 0.312) and low genetic differentiation among populations (FST = 0.053 y GST = 0.048). Its diversity and genetic structure are determined by the mating system and life history traits, more than by inhabiting aquatic environments.

Genetic diversity in red pine: evidence for low genic heterozygosity

1977 ◽  
Vol 7 (2) ◽  
pp. 343-347 ◽  
Author(s):  
D. P. Fowler ◽  
R. W. Morris
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Allozyme Variation ◽  
Red Pine ◽  
Starch Gel Electrophoresis ◽  
Banding Patterns ◽  
Coniferous Species ◽  
Low Degree ◽  
Starch Gel ◽  
Genetically Determined

Starch gel electrophoresis was used to survey for genetically determined enzyme mobility differences among 297 megagametophytes of red pine (Pinusresinosa Ait.) from five widely separated geographical sources. Consistent and reproducible enzyme banding patterns were observed with five of the seven isozyme systems assayed. No variation in band mobility was observed in any of these systems. This result stands in contrast with those reported from surveys of allozyme variation in other coniferous species but is consistent with the low degree of genetic variation observed in red pine for higher levels of genetic organization. It is concluded that red pine is genetically depauperate.Possible explanations for restricted genetic diversity are discussed. The most plausible explanation suggests that red pine was at sometime, possibly during the Pleistocene, reduced to a small refugial population and has yet to reestablish equilibrium heterozygosity.

Genetic structure and mating system of white spruce (Piceaglauca) in a seed production area

1984 ◽  
Vol 14 (5) ◽  
pp. 639-643 ◽  
Author(s):  
John N. King ◽  
Bruce P. Dancik ◽  
Narinder K. Dhir
Keyword(s):  
Genetic Structure ◽  
Mating System ◽  
Seed Production ◽  
Gel Electrophoresis ◽  
Random Mating ◽  
White Spruce ◽  
Starch Gel Electrophoresis ◽  
Starch Gel ◽  
Production Area ◽  
Mating Model

Embryos and megagametophytes of open-pollinated seed of 37 white spruce (Piceaglauca (Moench) Voss) trees from a seed production area were analyzed by starch gel electrophoresis to determine the genetic structure and mating system over 2 seed crop years. Analysis of four polymorphic enzyme loci (Gdh, Idh, Pgm, and Pgi-2) for spatial and temporal genetic structure and mating system indicated substantial deviations from the random mating model that is assumed when open-pollinated families are designated as half-sibs.

A Genetic Comparison of Sympatric Populations of Sand Lance (Genus Ammodytes) from the Region East of Cape Soya, Japan

1989 ◽  
Vol 46 (11) ◽  
pp. 1945-1951 ◽  
Author(s):  
Hiroaki Okamoto
Keyword(s):  
Genetic Structure ◽  
Gel Electrophoresis ◽  
Distinct Species ◽  
The Other ◽  
Starch Gel Electrophoresis ◽  
Sympatric Populations ◽  
Sand Lance ◽  
Protein Coding ◽  
Starch Gel ◽  
Genetic Comparison

Sand fance (Genus Ammodytes) collected from four stations off Japan and one station at Kodiak, Alaska were genetically characterized at 17 protein coding loci using starch-gel electrophoresis. Sand lance in Wakkanai (Cape Soya, Japan) consist of two genetically distinct groups. They are fixed for different alleles at four loci (Ldh-2, -3, G3pdh-2, and Mdhp-2). The genetic structure of one of the groups (Wakkanai-a group, W-a) is similar to that of A. personatus around Japan. The other group (Wakkanai-b group, W-b) has different genetic structure from either A. personatus or the Alaskan collection, which is presumed to belong to A. hexapterus. It is not presently possible to identify the affiliation of the W-b group; however, despite its sympatry with the W-a group, it is reproductively isolated and therefore is probably a distinct species occurring northeast of Hokkaido.

Changes in the genetic diversity of barley of Nordic and Baltic origin, studied by isozyme electrophoresis

2003 ◽  
Vol 1 (2-3) ◽  
pp. 143-149 ◽  
Author(s):  
Agnese Kolodinska Brantestam ◽  
Roland von Bothmer ◽  
Isaak Rashal ◽  
Jens Weibull
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Hordeum Vulgare L ◽  
Isozyme Electrophoresis ◽  
Breeding Lines ◽  
Total Genetic Diversity ◽  
Isozyme Loci ◽  
Starch Gel ◽  
The 19Th Century

AbstractIn this study an evaluation was made of changes in the genetic variation of barley (Hordeum vulgare L.) of Nordic and Baltic origin, from the end of the 19th century until today. A comparison of Nordic and Baltic material with exotic material that has not been subjected to intense selection was also made. A total of 293 accessions, including 160 Nordic and 80 Baltic landraces, cultivars and breeding lines, and another 53 landraces of exotic origin (Central Asia), were surveyed using isozyme starch gel electrophoresis. For the four isozymes studied, 28 alleles at 11 loci were observed. The average total genetic diversity value (HT) for individual loci ranged between 0 and 0.519. In the exotic material nine loci were polymorphic and two monomorphic, compared to seven polymorphic and four monomorphic loci in the Nordic and Baltic material. Some of the rare alleles were detected only in the exotic material. The studied isozyme loci of Nordic and Baltic material indicated that modern cultivars have a lower average genetic diversity value compared to the landraces, old and exotic material.

Sibling species in the mangrove genus Ceriops (Rhizophoraceae), detected using biochemical genetics*

1988 ◽  
Vol 1 (4) ◽  
pp. 391 ◽  
Author(s):  
ER Ballment ◽  
TJ III Smith ◽  
JA Stoddart
Keyword(s):  
Genetic Structure ◽  
Sibling Species ◽  
Gel Electrophoresis ◽  
Starch Gel Electrophoresis ◽  
Biochemical Genetics ◽  
Species Status ◽  
Phenotypic Characters ◽  
Starch Gel ◽  
Ceriops Tagal ◽  
Mangrove Trees

Starch gel electrophoresis of enzymes from the mangrove trees Ceriops tagal (Perr.) C. B. Robinson var. tagal, Ceriops tagal (Perr.) C. B. Robinson var. australis C. T. White, and Ceriops decandra (Griff.) Ding Hou in northern Queensland, Australia, revealed a uniform genetic structure within taxa, but high levels of genetic divergence among taxa. No more than 20% of enzyme phenotypic characters were shared by any pair of the taxa, and patterns of enzyme phenotypic variation consistent with hybridisation among them were not seen in any samples, including those from areas of spmpatry. This evidence of reproductive isolation is considered sufficient to accord species status to C. tagal var. australis.* Aust. lnst. Marine Sci. Contrib. No. 453.

High genetic diversity in a clonal relict Alexgeorgea nitens (Restionaceae): implications for ecological restoration

2010 ◽  
Vol 58 (3) ◽  
pp. 206 ◽  
Author(s):  
Elizabeth Sinclair ◽  
Siegfried Krauss ◽  
Belinda Cheetham ◽  
Richard Hobbs
Keyword(s):  
Genetic Diversity ◽  
Life History ◽  
Genetic Structure ◽  
Sexual Reproduction ◽  
Ecological Restoration ◽  
Population Genetic Structure ◽  
Population Genetic ◽  
Life History Traits ◽  
Pollen Dispersal ◽  
High Genetic Diversity

The importance of genetic issues associated with the sourcing of propagules is increasingly recognised for successful ecological restoration. A consideration of life history traits has contributed to ‘best-guess’ scenarios on the appropriate location and desirable properties of local provenance source populations, but these can lack precision. For clonal species, population genetic structure and variation will depend on the balance between the extent and growth rate of asexual clones, sexual reproduction, pollen dispersal, and subsequent seed dispersal and recruitment. We assessed patterns of population genetic structure and variation for Alexgeorgea nitens (Nees) L. Johnston & B. Briggs (Restionaceae), a dioecious, clonal, perennial species, with novel life history traits. Our results show high levels of genetic diversity within populations, and surprisingly low levels of population differentiation (ΦST = 0.17). We suggest that the high genetic diversity observed within these populations reflects extensive pollen dispersal and successful seeding (sexual reproduction) and recruitment events, even though direct observations of seedling recruitment are rare. In this case, a ‘best-guess’ propagule-sourcing scenario based on life-history traits that appear to limit dispersal capability does not predict the extent of high local genetic diversity and weak population genetic structure in A. nitens.

High genetic diversity in the only known population of Aechmea tuitensis (Bromeliaceae)

2000 ◽  
Vol 48 (5) ◽  
pp. 645 ◽  
Author(s):  
Liz Y. Izquierdo ◽  
Daniel Piñero
Keyword(s):  
Genetic Diversity ◽  
Gel Electrophoresis ◽  
Vegetative Reproduction ◽  
Clonal Diversity ◽  
Starch Gel Electrophoresis ◽  
High Genetic Diversity ◽  
Narrow Endemic ◽  
Expected Heterozygosity ◽  
Hardy Weinberg Equilibrium ◽  
Starch Gel

Aechmea tuitensis (P. Magaña and E. Lott) is a narrow endemic species from Mexico. Only one population has been reported. We studied the genetic variation and structure, and clonal diversity of this rare bromeliad by using starch gel electrophoresis. Six enzyme systems encoded by nine gene loci were resolved in nine subpopulations. The percentage of polymorphic loci was high (77.8%). Mean expected heterozygosity for the population was 0.12. All loci except CPX-1 were in Hardy–Weinberg equilibrium. Genetic diversity was substantially larger within subpopulations (mean Fis = 0.631) than between them (Fst = 0.196). We detected 33 genetically distinguishable clones from 109 plants (ramets) sampled. One clone had a frequency of 32.1%, others showed frequencies ranging from 1.8 to 9.2%, and 19% of the total samples were unique multilocus genotypes. Aechmea tuitensis exhibits high genetic diversity even though it has an extremely narrow distribution. Our results also suggest that the effect of genetic drift on genetic structure in A. tuitensis might be counteracted by the influence of vegetative reproduction.

Fractionation of Plasminogen by Starch Gel Electrophoresis

1964 ◽  
Vol 12 (01) ◽  
pp. 126-136 ◽  
Author(s):  
Karl H. Slotta ◽  
J. D Gonzalez
Keyword(s):  
Gel Electrophoresis ◽  
Room Temperature ◽  
Broad Band ◽  
Caproic Acid ◽  
Starch Gel Electrophoresis ◽  
Full Activity ◽  
Veronal Buffer ◽  
Starch Gel ◽  
Alkaline Range

SummaryWhen urea or ε-amino caproic acid were used as solublizing agents for plasminogen in electrophoretic experiments, only one broad band of the proenzyme was obtained on acetate cellulose, in starch block, and in acrylamide gel. In starch gel electrophoresis, however, both forms of plasminogen – the native or euglobulin and Kline’s or Pseudoglobulin plasminogen – separated into six bands. These migrated toward the cathode at room temperature in borate or veronal buffer in the alkaline range and showed full activity in fibrinagar-streptokinase plates.

scholarly journals THE INHERITANCE OF ENZYME VARIANTS FOR TYROSINE AMINOTRANSFERASE, NADP-DEPENDENT MALATE DEHYDROGENASE, NADP-DEPENDENT ISOCITRATE DEHYDROGENASE, AND TETRAZOLIUM OXIDASE IN TETRAHYMENA PYRIFORMIS, SYNGEN 1

Genetics ◽  
1973 ◽  
Vol 74 (4) ◽  
pp. 595-603
Author(s):  
D Borden ◽  
E T Miller ◽  
D L Nanney ◽  
G S Whitt
Keyword(s):  
Detailed Analysis ◽  
Malate Dehydrogenase ◽  
Isocitrate Dehydrogenase ◽  
Gel Electrophoresis ◽  
Tetrahymena Pyriformis ◽  
Tyrosine Aminotransferase ◽  
Breeding Program ◽  
Starch Gel Electrophoresis ◽  
Enzyme Locus ◽  
Starch Gel

ABSTRACT The isozymic patterns of tyrosine aminotransferase, NADP malate dehydrogenase, NADP isocitrate dehydrogenase, and tetrazolium oxidase were examined by starch-gel electrophoresis in Tetrahymena pyriformis, syngen 1. The genetics of the alleles controlling these enzymes was studied through a breeding program. Each enzyme locus was shown to assort vegetatively, as do other loci in this organism. A detailed analysis of the assortment process for the tyrosine aminotransferase locus indicated that the rate of stabilization of heterozygotes into pure types was essentially identical to previously-reported rates for other loci.

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