Noninvasive studies of conifer roots: nuclear magnetic resonance (NMR) imaging of Douglas-fir seedlings

1991 ◽  
Vol 21 (11) ◽  
pp. 1559-1566 ◽  
Author(s):  
Douglas P. Brown ◽  
Thomas K. Pratum ◽  
Caroline Bledsoe ◽  
E. David Ford ◽  
J. Steven Cothern ◽  
...  

An artificial soil mix suitable for magnetic resonance imaging was developed. This soil mix, consisting of quartz sand, peat moss, and kaolinite clay (5:3:2, by volume), has a natural texture and consistence and produces high-contrast images in soil media with up to 15% water content. Douglas-fir (Pseudotsugamenziesii (Mirb.) Franco) seedlings were grown in this soil mix in 12.5 cm diameter containers and periodically imaged over a 12-month period. These images showed roots growing undisturbed in the soil mix and initiation of numerous lateral roots. From these images, root lengths and growth rates were calculated. Calculated root lengths were in close agreement with measured lengths of excavated roots. Undisturbed lateral roots had measured growth rates of about 1 mm/day. Roots that reached the container wall and then grew down along the wall had significantly higher growth rates, over 2 mm/day. This phenomenon may have important implications for growth rate data from root window and minirhizotron experiments.

2006 ◽  
Vol 60 (3) ◽  
pp. 380-383 ◽  
Author(s):  
Johan Pallud ◽  
Emmanuel Mandonnet ◽  
Hugues Duffau ◽  
Michèle Kujas ◽  
Rémy Guillevin ◽  
...  

1990 ◽  
Vol 70 (4) ◽  
pp. 1151-1154 ◽  
Author(s):  
K. STELWAGEN ◽  
B. W. McBRIDE ◽  
D. G. GRIEVE ◽  
R. A. TOWNER

Mammary glands of eight nonpregnant, nulliparous Holstein heifers (416 ± 11 d) were used to study the potential for nuclear magnetic resonance (NMR) imaging and proton spectroscopy as a technique to assess mammary gland composition. It was concluded that the NMR technique has the potential to quantitate mammary gland composition in heifers. Key words: Nuclear magnetic resonance imaging and spectroscopy, mammary composition, Holstein heifers


1985 ◽  
Vol 5 (2) ◽  
pp. 267-274 ◽  
Author(s):  
Hiroyuki Kato ◽  
Kyuya Kogure ◽  
Hitoshi Ohtomo ◽  
Muneshige Tobita ◽  
Shigeru Matsui ◽  
...  

Evaluation of ischemic brain injury in experimental cerebral infarction in gerbils and rats was performed by means of both proton nuclear magnetic resonance imaging ([1H]NMR-CT) and various histochemical analyses. In vivo nuclear magnetic resonance (NMR) imaging was carried out employing saturation recovery, inversion recovery, and spin echo pulse sequences. Spatial resolution of the images was excellent. The ischemic lesions were detected with a remarkable contrast in inversion recovery and spin echo images within a few hours after insult. Those changes in NMR images consistently corresponded with the various retrospective histochemical observations, especially with methods related to brain edema (K+ staining) rather than structural (enzymatic) studies. Calculated T1 and T2 relaxation times indicated the evolution of the edema state in the brain in situ. They correlated excellently with the retrospective water content measurement. As a result, detailed characterization of the edema state induced by cerebral ischemia was possible in vivo using [1H]NMR imaging.


2007 ◽  
Vol 22 (6) ◽  
pp. 1-10 ◽  
Author(s):  
Tarvinder Singh ◽  
Michel Kliot

Recent advancements in the field of imaging, particularly magnetic resonance imaging, have improved the way peripheral nerve tumors are diagnosed, monitored, and treated both medically and surgically. When combined with the all-important clinical evaluation and electrodiagnostic studies, imaging permits a clinician to accomplish the following: identify the size, shape, location, and distribution of mass lesions; infer the risks in resecting a mass lesion by displaying its relationship to nearby nerve fascicles; determine growth rates when serial imaging studies are available; suggest the nature of the pathological entity; and visualize muscle denervation associated with nerve damage. The authors describe some of the commonly used imaging modalities and discuss their use in evaluating and treating peripheral nerve tumors and other mass lesions.


1982 ◽  
Vol 35 (6) ◽  
pp. 761 ◽  
Author(s):  
JM Pope

The principles of two new techniques of medical physics, nuclear magnetic resonance (NMR) imaging and topical magnetic resonance, are outlined. Progress in the development of these techniques and their application in clinical trials is reviewed. Advantages of NMR methods over existing imaging modalities are discussed. Finally some safety aspects are considered.


1987 ◽  
Vol 42 (9) ◽  
pp. 1037-1040 ◽  
Author(s):  
M. Braun ◽  
O. Lutz ◽  
W. I. Jung ◽  
C. S. Kischkel ◽  
R. Oeschey ◽  
...  

Nuclear magnetic resonance imaging has been perform ed with the nuclei 27A1, 51V, and 23Na in some test objects with a 1.5 T imager. Encouraging signal-to-noise ratios have been obtain ed within reasonable measuring times in the imaging and the spectroscopy mode using a commercially available 23Na headcoil.


2002 ◽  
Vol 82 (3) ◽  
pp. 513-519 ◽  
Author(s):  
L. N. Pietrzak ◽  
J. Frégeau-Reid ◽  
B. Chatson ◽  
B. Blackwell

Water in seeds plays an important role not only in physiological but also in chemical processes. In addition to the requirements of water for germination, seeds of legumes used for human consumption require hydration to prepare them for cooking. The site of water entry, however, and its movement during imbibition in legumes and particularly in soybean is still not clear. One of the best and most precise methods of tracing water movement in plant tissue is nuclear magnetic resonance (NMR) imaging. In our study, we applied NMR imaging to reveal the water distribution in soybean seeds during the first 24 h of hydration. It has been found that hydration during this period is a multistage process. Water enters the seed through the micropyle and hilum and the concentration of water there is very high during the entire imbibition process. Inside the seed, water first fills the voids between cotyledons, and between the cotyledons and the seed coat. Water then enters the embryonic axis, and from it, is distributed into cotyledons. The highest water concentration after 24 h of imbibition was observed in the embryonic axis. The external part of the cotyledons was hydrated at a slower rate than the internal tissue. Key words: Soybean, Glycine max L., nuclear magnetic resonance imaging, water imbibition, water distribution


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