Accuracy of the tritium water dilution method for determining water flux in reindeer (Rangifer tarandus)

1976 ◽  
Vol 54 (6) ◽  
pp. 857-862 ◽  
Author(s):  
R. D. Cameron ◽  
R. G. White ◽  
J. R. Luick
Keyword(s):  
Total Body Water ◽  
Crude Protein ◽  
Tritiated Water ◽  
Water Flux ◽  
Body Water ◽  
Water Volume ◽  
Dilution Method ◽  
Wide Range ◽  
Total Body ◽  
Water Dilution

The accuracy of the tritium water dilution method in estimating water flux was evaluated in reindeer under various conditions of temperature and diet. Two non-pregnant female reindeer were restrained in metabolism stalls, within controlled-environment chambers, at temperatures of + 10, −5, and −20 °C; varying amounts of a commercial pelleted ration (crude protein, 13%) or mixed lichens (crude protein, 3%) were offered, and water was provided ad libitum either as snow or in liquid form. Total body water volume and water turnover were estimated using tritiated water, and the daily outputs of feces and urine were measured for each of 12 different combinations of diet and temperature. Statistical analysis of the data showed that the tritium water dilution technique gives accurate determinations of total body water flux over a wide range of environmental and nutritional conditions.

Seasonal changes in total body water, extracellular fluid, and blood volume in grazing reindeer

1972 ◽  
Vol 50 (1) ◽  
pp. 107-116 ◽  
Author(s):  
R. D. Cameron ◽  
J. R. Luick
Keyword(s):  
Body Weight ◽  
Blood Volume ◽  
Total Body Water ◽  
Tritiated Water ◽  
Extracellular Fluid ◽  
Body Water ◽  
Late Spring ◽  
Water Volume ◽  
Sodium Chromate ◽  
Total Body

The effects of climatic and nutritional changes on body fluid compartmentalization and turnover were investigated in grazing female reindeer. Total body water volume and turnover, extracellular fluid volume, and blood volume were estimated using tritiated water, sodium sulfate-35S, and sodium chromate-51Cr, respectively. During winter and spring, body weights were either maintained or reduced while total body water (percentage of body weight) increased, resulting in appreciable losses of total body solids. In summer, large gains in body weight were accompanied by reduced total body water volumes resulting in substantial increases in body solids. An apparent fluid shift from the intravascular to the extracellular compartment during late spring suggested the occurrence of a starvation edema. Mean water flux rates (ml/day per kilogram body weight) were higher in late spring than during other seasons; lowest values were recorded in early winter. Seasonal variations in nutritional status as reflected by body composition and fluid compartmentalization, and changes in water turnover are discussed in relation to climate and the quality and availability of forage. The complicating influences of pregnancy and lactation are also considered.

Can tritiated water-dilution space accurately predict total body water in chukar partridges?

1985 ◽  
Vol 59 (5) ◽  
pp. 1383-1388 ◽  
Author(s):  
B. G. Crum ◽  
J. B. Williams ◽  
K. A. Nagy
Keyword(s):  
Body Mass ◽  
Total Body Water ◽  
Tritiated Water ◽  
Body Water ◽  
Dry Matter Content ◽  
Constant Mass ◽  
Oven Drying ◽  
Total Body ◽  
Water Dilution ◽  
Chukar Partridges

Total body water (TBW) volumes determined from the dilution space of injected tritiated water have consistently overestimated actual water volumes (determined by desiccation to constant mass) in reptiles and mammals, but results for birds are controversial. We investigated potential errors in both the dilution method and the desiccation method in an attempt to resolve this controversy. Tritiated water dilution yielded an accurate measurement of water mass in vitro. However, in vivo, this method yielded a 4.6% overestimate of the amount of water (3.1% of live body mass) in chukar partridges, apparently largely because of loss of tritium from body water to sites of dissociable hydrogens on body solids. An additional source of overestimation (approximately 2% of body mass) was loss of tritium to the solids in blood samples during distillation of blood to obtain pure water for tritium analysis. Measuring tritium activity in plasma samples avoided this problem but required measurement of, and correction for, the dry matter content in plasma. Desiccation to constant mass by lyophilization or oven-drying also overestimated the amount of water actually in the bodies of chukar partridges by 1.4% of body mass, because these values included water adsorbed onto the outside of feathers. When desiccating defeathered carcasses, oven-drying at 70 degrees C yielded TBW values identical to those obtained from lyophilization, but TBW was overestimated (0.5% of body mass) by drying at 100 degrees C due to loss of organic substances as well as water.

Body composition of live cattle and its prediction from fasted liveweight, tritiated water space and age

1982 ◽  
Vol 33 (2) ◽  
pp. 375 ◽  
Author(s):  
PR Bird ◽  
PC Flinn ◽  
JWD Cayley ◽  
MJ Watson
Keyword(s):  
Body Composition ◽  
Total Body Water ◽  
Tritiated Water ◽  
Protein A ◽  
Body Water ◽  
The Body ◽  
Linear Regression Models ◽  
Wide Range ◽  
Total Body ◽  
Water Space

The body composition of 23 Hereford steers was related to their liveweight after a 24 h fast (W), total body water (TBW), tritiated water space (T) and age in months (A) by using allometric or linear regression models. Fifteen steers, of similar initial age, were taken at intervals from a grazing experiment having a wide range of stocking rates. These steers were 15-22 months of age and 189-461 kg W at slaughter. Six younger and two older steers were also used to expand the range to 3-33 months and 90-517 kg. The steers were taken from pasture 3 h after sunrise and deprived of feed and water thereafter. After 4 h, tritiated water was given intramuscularly and 20 h later blood was collected and the animals were weighed and slaughtered. Models having the least residual standard deviation (r.s.d.) and the predictors which contributed significantly in the stepwise regression analysis (P i 0.05) are given for each range of W. T greatly improved the estimation of fat and slightly improved the prediction of protein. A only slightly improved the prediction of TBW and fat. Equations applicable to the narrow range of W were: total body water (kg) =

scholarly journals Seasonal changes in total body water; body composition and water turnover in reindeer

Rangifer ◽  
1985 ◽  
Vol 5 (1) ◽  
pp. 2 ◽  
Author(s):  
Terje S. Larsen ◽  
Arnoldus Schytte Blix
Keyword(s):  
Body Weight ◽  
Food Intake ◽  
Ambient Temperature ◽  
Body Fat ◽  
Total Body Water ◽  
Tritiated Water ◽  
Body Water ◽  
Dilution Method ◽  
Water Turnover ◽  
Total Body

<p>Total body water and water turnover were measured at different times throughout the year in 3 captive Norwegian reindeer, using a tritiated water dilution method (Holleman et al. 1982). Total body water (percent of body weight) increased during late autumn and winter, from 59.1 &plusmn; 1.5 % in October to 72.5 &plusmn; 2.0 % in April. Using the equatation by Pace and Rathbun (1945) for predicting total body fat (% fat = 100 - % water/0.732), this increase in total body water indicates a concomitant reduction in body fat, from a maximum value of 18.9 &plusmn; 2.6 % (of body weight) in October to a minimum of 0.9 &plusmn; 2.7 % in April. During summer, on the other hand, fat content increased at the expense of a reduced percentage of body water. Water turnover was low in winter (December - April), ranging between 30.8 &plusmn; 5.2and43.6 &plusmn; 13.5ml.d-'. kg-1, but increased nearly fourfold during summer (June-August) with a maximum of 117.7 &plusmn; 5.9 ml.d-1. kg-1 in August. Positive correlations between water turnover and food intake and between water turnover and ambient temperature were found, the latter probably resulting from an incidental correlation between food intake and ambient temperature.</p><p>Sesongmessige forandringer i totalt kroppsvann, kropps-sammensetning og vannomsetning hos reinsdyr.</p><p>Abstract in Norwegian / Sammendrag: Totalt kroppsvann og vannomsetning av vann ble m&aring;lt til forskjellige &aring;rstider i 3 norske reinsdyr ved hjelp av utvasking av tritiert vann (Holleman et al. 1982). Totalt kroppsvann (prosent av kroppsvekt) &oslash;kte utover h&oslash;sten og vinteren, fra 59.1 &plusmn; 1.5 % i oktober til 72.5 &plusmn; 2.0 % i april. Ved hjelp av en ligning som er gitt av Pace og Rathbun (1945) for beregning av totalt kroppsfett (% fett = 100 - % vann/0.732), fant en at denne &oslash;kningen i vanninnhold tilsvarte en samtidig reduksjon i fettinnhold, fra en maksimums-verdi p&aring; 18.9 &plusmn; 2.6 % av kroppsvekt i oktober til et minimum p&aring; 0.9 &plusmn; 2.7 % i april. Utover sommeren &oslash;kte derimot innholdet av fett p&aring; bekostning av vanninnholdet. Omsetningen av vann var lav vinterstid (desember - april), varierende mellom 30.8 &plusmn; 5.2 og 43.6 &plusmn; 13.5 ml.d-1.kg-1, men &oslash;kte nesten fire ganger i l&oslash;pet av sommeren (juni - august) til et maksimum p&aring; 117.7 &plusmn; 5.9 ml.d-1.kg-1 i august. Det ble funnet positive korrelasjoner mellom vannomsetning og forinntak og mellom vannomsetning og omgivelsestemperatur. Sistnevnte korrelasjon kan muligens skyldes en tilfeldig sammenheng mellom forinntak og omgivelsestemperatur.</p><p>Vuodenaikaiset muutokset poron ruumiin kokonaisvesim&aring;&aring;r&aring;ss&aring;, ruumiin koostumuksessa ja vesiaineenvaihdunnassa.</p><p>Abstract in Finnish / Yhteenveto: Ruumiin kokonaisvesima&aring;r&aring;&aring; ja vesiaineenvaihduntaa mitattiin eri vuodenaikoina 3 norjalaisella porolla k&aring;ytt&aring;m&aring;ll&aring; apuna tritioitua vetta (Holleman et al. 1982). Ruumiin kokonaisvesim&aring;ar&aring; (prosenttia ruumiinpainosta) lis&aring;antyi syksyll&aring; ja talvella lokakuun 59.1&plusmn;1.5%:sta 72.5&plusmn;2.0%:i huhtikuussa. K&aring;ytt&aring;m&aring;ll&aring; Pacen ja Rathbunin (1945) ruumiin kokonaisrasvapitoisuude laskukaavaa (rasva % = 100 - vesi %/0.732) huomattiin tam&aring;n vesim&aring;&aring;r&aring;n lisa&aring;ntymis johtuvan samanaikaisesta rasvapitoisuuden v&aring;henemisesta. Rasvapitoisuus laski lokakuun maksimiarvosta 18.9&plusmn;2.6% ruumiinpainosta huhtikuun minimiarvoon, joka oli 0.9&plusmn;2.7% ruumiinpainosta. Kes&aring;ll&aring; rasvapitoisuus lis&aring;antyi puolestaan vesipitoisuuden kustannuksella. Talvella j&aring;k&aring;l&aring;ravinnolla (joulu-huhtikuussa) veden kaytto vaihteli v&aring;lilla 30.8&plusmn;5.2 ja 43.6&plusmn;13.5 ml vrk-1kg-1 mutta se kohosi melkein nelinkertaisesti kesalla (kes&aring;-elokuussa) maksimiarvoonsa 117.7+5.9 ml vrk-1 kg-1 elokuussa. Veden k&aring;yton ja ravinnon oton seka veden k&aring;yton jaymp&aring;riston lampotilan v&aring;lilla oli positiivinen korrelaatio. Viimeksi mainittu korrelaatio voi johtua v&aring;liaikaisesta riippuvuudesta ravinnonoton ja ymp&aring;riston l&aring;mpotilan v&aring;lill&aring;.</p>

Total body water distribution of creatinine and urea in nephrectomized dogs

1960 ◽  
Vol 199 (4) ◽  
pp. 661-665 ◽  
Author(s):  
Paul R. Schloerb
Keyword(s):  
Total Body Water ◽  
Water Distribution ◽  
Tritiated Water ◽  
Body Water ◽  
Water Volume ◽  
Arterial Blood ◽  
Constant Rate ◽  
Total Body ◽  
Volumes Of Distribution ◽  
Isotopic Water

It was the purpose of this study to measure and to compare the volumes of distribution of creatinine, urea and tritiated water in nephrectomized dogs. After bilateral nephrectomy a solution containing known amounts of these was infused intravenously and at a constant rate in some studies. Frequent arterial blood samples were taken during and following infusion for periods up to 20 hours and were analyzed for water and the infused substances. The respective volumes of distribution and rates of dilution were calculated. Isotopic water and urea were distributed in 95% and creatinine in 61% of their final volumes of distribution at the end of a 30-minute infusion. Final equilibrium occurred in about 90 minutes with water and urea and in about 4 hours with creatinine. Creatinine became distributed in 99 ± 3% of the tritiated water volume and in 101 ± 5% of the urea volume. Urea distributed in 98 ± 4% of the tritiated water volume. It is concluded that administered creatinine is distributed in total body water of nephrectomized dogs with an equilibrium time of about 4 hours and that urea is similarly distributed within 1 1/2 hours.

Estimation of water content by tritium dilution of animals subjected to rapid live-weight changes

1969 ◽  
Vol 72 (1) ◽  
pp. 31-40 ◽  
Author(s):  
W. R. McManus ◽  
R. K. Prichard ◽  
Carolyn Baker ◽  
M. V. Petruchenia
Keyword(s):  
Body Weight ◽  
Water Content ◽  
Total Body Water ◽  
Compensatory Growth ◽  
Specific Activity ◽  
Tritiated Water ◽  
Body Water ◽  
Under Nutrition ◽  
The Mean ◽  
Total Body

SUMMARYThe use of tritiated water to estimate total body-water content of animals experiencing recovery from under-nutrition was studied.The time for equilibration of tritiated water (TOH), given intraperitoneally, with total body water (TBW) was determined in rabbits and in rats. As judged by the specific activity of blood water, equilibration had occurred by 76–125 min in the rabbit and did not appear to be affected by the plane of nutrition. However, between slaughter groups the specific activity of water obtained from the liver 180 min after injection of TOH was significantly different from the specific activity of water simultaneously obtained from the blood plasma. It is concluded that the liver is not a suitable tissue to use for testing achievement of equilibration.As judged by the specific activity of blood water compared to that of water from the whole body macerate, equilibration in mature rats either in stable body condition or undergoing rapid compensatory growth occurred in less than 60 min.A trial comparing TOH-space (corrected by 3% body weight) and actual TBW (by desiccation) was conducted on thirty rabbits which experienced under-nutrition followed by compensatory growth.Prior to under-nutrition the agreement between actual and estimated TBW was satisfactory and within 2·3%. During compensatory growth the agreement was poor— the TOH values over-estimating actual TBW by about 12%.A trial with mature rats confirmed the findings with rabbits. For rats in stable body weight the mean estimated TOH-space for fourteen animals was within 1·2% of the actual TBW. For fourteen rats undergoing compensatory growth the mean estimated TOH-space (corrected by 3% body weight) overestimated actual TBW by 6·2%.

Body composition in vivo. II. The composition of mature goats and its relationship to the antipyrine, tritiated water, and N-acetyl-4-aminoantipyrine spaces

1963 ◽  
Vol 14 (6) ◽  
pp. 926 ◽  
Author(s):  
BA Panaretto ◽  
AR Till
Keyword(s):  
Body Composition ◽  
Total Body Water ◽  
Tritiated Water ◽  
Body Water ◽  
Total Body

The antipyrine, tritiated water, and N-acetyl-4-aminoantipyrine spaces were determined simultaneously in goats which had been deprived of feed and water for 48 hr. The animals were then killed, minced, and analysed for water, fat, protein, and ash contents. The compositions of the whole and empty bodies of the goats were calculated, and the relationships between the bodily components were compared with those reported for cattle, sheep, and some monogastric species. The relationships found between the components of the whole bodies compared favourably with those derived from the empty bodies. The relationships of the spaces determined in vivo to total body water, fat, and protein were found, and confidence statements were placed on predicted estimates.

scholarly journals The prediction of body composition in poultry by estimation in vivo of total body water with tritiated water and deuterium oxide

1988 ◽  
Vol 59 (1) ◽  
pp. 109-124 ◽  
Author(s):  
R. J. Johnson ◽  
D. J. Farrell
Keyword(s):  
Body Composition ◽  
Isotope Dilution ◽  
Total Body Water ◽  
Deuterium Oxide ◽  
Tritiated Water ◽  
Live Weight ◽  
Body Water ◽  
Regression Equations ◽  
Total Body

1. Birds (n169) which varied in age, live weight, nutritional history, physiological state and genotype were slaughtered and analysed for total body water. Before slaughter, birds were injected with the water isotopes tritiated water (TOH) or deuterium oxide (D2O), or both, to determine TOH space or D2O space, or both, as estimates of total body water in vivo.2. At the mean total body water of all birds determined by desiccation, of 1096·4 (SD 424·1) g, TOH space and D2O space overestimated total body water by 10·4 and 8·5 % respectively. The difference between the isotopes was significant (P< 0·05).3. Based on recovery of isotope it was postulated that the main reason for the observed overestimation of total body water in vivo was incomplete recovery of isotope due to the vacuum sublimation technique. The mean recovery (%) of added isotope to whole blood after vacuum sublimation was 93·0 (SD 2·6) and 92·4 (SD 5·5) of the theoretical concentrations of TOH and D2O respectively.4. Nevertheless, accurate prediction of total body water was obtained from regression equations which included live weight and isotope-dilution space. Values required logarithmic (base 10) transformation before derivation of linear and multiple linear regression equations, and the precision of prediction was determined by the residual standard deviation (RSD).5. Total body water could be predicted with nearly equal accuracy from live weight or isotope-dilution space (RSD 0·025 and 0·020 respectively). Prediction of carcass protein was more accurate from live weight (RSD 0·033) than from TOH space (RSD 0·036), and inclusion of both variables resulted in only a marginal decrease in RSD to 0·031.6. The prediction of carcass fat and energy was markedly improved by the inclusion of isotope-dilution space in conjunction with live weight compared with live weight alone.7. The relations show the developmental nature of body composition of domestic fowl given diets adequate in nutrients. The prediction equations demonstrate the precision possible for studies in which estimates of body composition in poultry are required without slaughter.

Estimation of body water and fat in cattle using tritiated water space and live weight with particular reference to the influence of breed

1983 ◽  
Vol 101 (2) ◽  
pp. 257-264 ◽  
Author(s):  
P. R. N. Chigaru ◽  
D. H. Holness
Keyword(s):  
Body Fat ◽  
Total Body Water ◽  
Tritiated Water ◽  
Live Weight ◽  
Close Relation ◽  
Body Water ◽  
The Body ◽  
Metabolizable Energy ◽  
Complete Diet ◽  
Total Body

SUMMARYThe body composition of 18 each of Mashona, Afrikaner and Hereford heifers was measured at the beginning and after 16 and 32 weeks of the experiment. The heifers not slaughtered at the beginning of the experiment were fed a complete diet containing 132 g crude protein and 12·0 MJ metabolizable energy/kg dry matter. Before slaughter, the animals were deprived of food and water for 24 h. Each animal was infused with 1 mCi of tritiated water (TOH) in order to measure total body water (TBW) and to estimate body fat.The growth rate of the three breeds of heifers was similar despite differences in age and initial live weight. Both TBW and fat proportions, however, differed significantly (P < 0·01) between slaughter stages for each breed and between breeds at each slaughter stage. At the first, second and final slaughter stages the proportions of TBW were: 68·0, 59·4 and 54·5% for Mashona; 70·;5, 64·3 and 58·3% for Afrikaner and 65·3, 57·6 and 46·2% for Hereford heifers respectively. The corresponding proportions of body fat were: 10·2, 18·4 and 24·2% for Mashona; 6·6, 12·0 and 20·0% for Afrikaner and 13·7, 20·8 and 25·8% for Hereford heifers respectively.There was a close relation between empty body weight and live weight at slaughter which was not influenced by breed. Both TBW and fat were estimated more accurately when TOH space and live weight were used jointly. However, the slopes of the prediction equations for each breed were significantly different (P < 0·05) in the case of both total body water and fat. It was necessary to use separate equations for each breed in order to predict either body water or fat. The significance of these findings for the estimation of body fat in live cattle is discussed.

Sign in / Sign up

Export Citation Format

Share Document