Hematological characterization of goldfish, Carassius auratus L., by image analysis: effects of thermal acclimation and heat shock

The morphological characteristics of erythrocyte populations from goldfish, Carassius auratus L., acclimated to constant (15, 25, or 35 °C) and diurnally cycling (25 ± 10 °C) temperature regimes or exposed to abrupt heat shock (15–25 °C, 25–35 °C) were examined by image analysis in an attempt to develop criteria for assessing the stage of development and red cell population age structure. Of the indices considered, nuclear form factor (4π area/perimeter2) appeared to best define the immature state. Estimates of juvenile cell abundances based on nuclear form factor ranged from 23.2 to 56.5% of the cell population and were generally consistent with the thermal histories of the test groups examined. Erythrocyte population characterization is recommended for inclusion in future studies on hematological features of response to altered environmental conditions.

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Morphological differences in embryos of goldfish (Carassius auratus auratus) under different incubation temperatures

The International Journal of Developmental Biology ◽

10.1387/ijdb.190144hu ◽

2019 ◽

Vol 63 (11-12) ◽

pp. 597-604 ◽

Cited By ~ 1

Author(s):

Hirotaro Urushibata ◽

Eisuke Takahashi ◽

Yu Shimizu ◽

Toshiya Miyazaki ◽

Takafumi Fujimoto ◽

...

Keyword(s):

Carassius Auratus ◽

Morphological Characteristics ◽

Temperature Tolerance ◽

Cell Stage ◽

Animal Pole ◽

Goldfish Carassius Auratus ◽

Morphological Differences ◽

Incubation Temperatures ◽

Single Batch

The goldfish (Carassius auratus auratus) is a useful species for embryonic micromanipulations because of its large egg size and wide temperature tolerance. Here, we describe in detail the rate of development and morphological characteristics of goldfish embryos incubated at temperatures between 10 °C and 30 °C. The cleavage speed increased rapidly as temperature increased. Synchronized cell divisions occurred at 131 min intervals at 10ºC, at 33 min intervals at 20 °C, and at 19 min intervals at 30 °C during the cleavage period. The rate of hatched abnormal embryos significantly increased at temperatures of 26 °C and above, while there was no change in the number of abnormal embryos at temperatures less than 24 °C. Moreover, the blastomeres around the center of the blastodisc rose in the direction of the animal pole at temperatures less than 14 °C. At the lower temperatures, clusters of maternally-supplied germplasm were visualized both at the ends of the first three cleavage furrows and at the border between the lower and upper tiers at the 16- to 32-cell stage, with injection of artificial mRNA and vasa in situ hybridization. This study showed that temperature affects not only developmental speed but also the shape of the blastodisc and the distribution of maternally-supplied materials in the blastodisc. By controlling the temperature, it is possible for researchers to prepare many stages of embryos and shapes of the blastodisc from a single batch of eggs.

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The identification of heat shock protein genes in goldfish (Carassius auratus) and their expression in a complex environment in Gaobeidian Lake, Beijing, China

Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology ◽

10.1016/j.cbpc.2007.01.018 ◽

2007 ◽

Vol 145 (3) ◽

pp. 350-362 ◽

Cited By ~ 18

Author(s):

Jianshe Wang ◽

Yanhong Wei ◽

Xuemei Li ◽

Hong Cao ◽

Muqi Xu ◽

...

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Carassius Auratus ◽

Complex Environment ◽

Goldfish Carassius Auratus ◽

Heat Shock Protein Genes ◽

Beijing China

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Histophysiology and innervation of the pituitary gland of the goldfish, Carassius auratus L.: a light and electron microscope investigation

Canadian Journal of Zoology ◽

10.1139/z72-113 ◽

1972 ◽

Vol 50 (6) ◽

pp. 835-844 ◽

Cited By ~ 58

Author(s):

John F. Leatherland

Keyword(s):

Electron Microscope ◽

Basement Membrane ◽

Carassius Auratus ◽

Secretory Cell ◽

Morphological Characteristics ◽

Cell Types ◽

Pars Intermedia ◽

Electron Microscope Investigation ◽

Goldfish Carassius Auratus ◽

Granule Release

Six secretory cell types (prolactin-, ACTH-, somatotropin- (STH-), TSH-, and two presumptive gonadotropin- (GTH-) secreting cells) were identified in light or electron microscope preparations of goldfish pars distalis. A further two cell types of unknown function were demonstrated in the pars intermedia. Cellular identification was based on the position of cells within the adenohypophysis and on the staining properties or morphological characteristics of the cytoplasmic granules.The prolactin-, ACTH-, and STH-secreting cells and the pars intermedia cells appeared to release granules into the basement membrane whereas granule release from TSH- and GTH-secreting cells was not found.Many of the adenohypophysial cell types were innervated directly or across a basement membrane by B-type neurohypophysial fibers. A-type neurosecretory fibers were associated with blood vessels and pituicytes within the neurohypophysis. The possible role(s) of the two types of pituicytes is discussed.

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A Drastic Reduction in the Basal Level of Heat-shock Protein 90 in the Brain of Goldfish (Carassius auratus) after Administration of Geldanamycin

ZOOLOGICAL SCIENCE ◽

10.2108/zsj.21.1085 ◽

2004 ◽

Vol 21 (11) ◽

pp. 1085-1089 ◽

Cited By ~ 5

Author(s):

Nao Kagawa

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Carassius Auratus ◽

Heat Shock Protein 90 ◽

Drastic Reduction ◽

Goldfish Carassius Auratus ◽

The Brain

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Identification, tissue distribution and characterization of two heat shock factors (HSFs) in goldfish (Carassius auratus)

Fish & Shellfish Immunology ◽

10.1016/j.fsi.2015.01.004 ◽

2015 ◽

Vol 43 (2) ◽

pp. 375-386 ◽

Cited By ~ 8

Author(s):

So-Sun Kim ◽

Ziwei Chang ◽

Jang-Su Park

Keyword(s):

Heat Shock ◽

Tissue Distribution ◽

Carassius Auratus ◽

Heat Shock Factors ◽

Goldfish Carassius Auratus

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SEM of Hepatocytes and Biliary Passages in Goldfish Liver

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100080213 ◽

1977 ◽

Vol 35 ◽

pp. 556-557

Author(s):

Waykin Nopanitaya ◽

Joe W. Grisham ◽

Johnny L. Carson

Keyword(s):

Carassius Auratus ◽

Cell Layer ◽

Three Dimensional ◽

Cell Types ◽

Biliary System ◽

Fish Food ◽

Commercial Fish ◽

Goldfish Carassius Auratus ◽

Commercial Fish Food

An interesting feature of the goldfish liver is the morphology of the hepatic plate, which is always formed by a two-cell layer of hepatocytes. Hepatic plates of the goldfish liver contain an infrequently seen second type of cell, in the centers of plates between two hepatocytes. A TEH study by Yamamoto (1) demonstrated ultrastructural differences between hepatocytes and centrally located cells in hepatic plates; the latter were classified as ductule cells of the biliary system. None of the previous studies clearly showed a three-dimensional organization of the two cell types described. In the present investigation we utilize SEM to elucidate the arrangement of hepatocytes and bile ductular cells in intralobular plates of goldfish liver.Livers from young goldfish (Carassius auratus), about 6-10 cm, fed commercial fish food were used for this study. Hepatic samples were fixed in 4% buffered paraformaldehyde, cut into pieces, fractured, osmicated, CPD, mounted Au-Pd coated, and viewed by SEM at 17-20 kV. Our observations were confined to the ultrastructure of biliary passages within intralobular plates, ductule cells, and hepatocytes.

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